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The map label for this gene is gcvH [H]

Identifier: 187734954

GI number: 187734954

Start: 531100

End: 531480

Strand: Direct

Name: gcvH [H]

Synonym: Amuc_0447

Alternate gene names: 187734954

Gene position: 531100-531480 (Clockwise)

Preceding gene: 187734953

Following gene: 187734955

Centisome position: 19.94

GC content: 54.86

Gene sequence:

>381_bases
ATGCACGACGTACCAGAAAATCTGCTGTATTCCAAAGATCACGAATGGATTGAAATTGACGGGGACATCGGCACCATCGG
CATTTCCGACCATGCCCAGGCCGAACTCTCCGATGTCGTCTTTGTAGACCTTCCCGAAGTAGGCGCCACCGTCGCAGCCG
GCGACCCTGTCGCTGTCGTGGAATCTGTCAAAGCCGCCAGCGACGTGTACACCCCCGTTTCAGGAGAAATCCTGGAAGTG
AACGAAGAGCTTTCCAATGATCCGTCCCTCATTAACTCCGACCCCTACGGCGCAGGCTGGCTGTACAAAATCCGCCTGGA
CGTTCCCACGGAAACGGAAGATATGATGAACGCCACGGATTACGAGGAATACTGCTCCTGA

Upstream 100 bases:

>100_bases
GTAAAAAAACCCTTTTATAAAAAAGGCTGACACTACGGCGCAAAAATAAACTGACAAACACCTCATTTTACTACACTCTC
ACTTCAAACAATTACATCTT

Downstream 100 bases:

>100_bases
ACCATTCTTCCCTCCCCCGCCCCGGTGATTCCATCCCGGGGCATTTTGTTTGGCCCGTTCCGCGGCCGGACGCTATTACC
AGCCAGAGATACCACATGAA

Product: glycine cleavage system H protein

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 126; Mature: 126

Protein sequence:

>126_residues
MHDVPENLLYSKDHEWIEIDGDIGTIGISDHAQAELSDVVFVDLPEVGATVAAGDPVAVVESVKAASDVYTPVSGEILEV
NEELSNDPSLINSDPYGAGWLYKIRLDVPTETEDMMNATDYEEYCS

Sequences:

>Translated_126_residues
MHDVPENLLYSKDHEWIEIDGDIGTIGISDHAQAELSDVVFVDLPEVGATVAAGDPVAVVESVKAASDVYTPVSGEILEV
NEELSNDPSLINSDPYGAGWLYKIRLDVPTETEDMMNATDYEEYCS
>Mature_126_residues
MHDVPENLLYSKDHEWIEIDGDIGTIGISDHAQAELSDVVFVDLPEVGATVAAGDPVAVVESVKAASDVYTPVSGEILEV
NEELSNDPSLINSDPYGAGWLYKIRLDVPTETEDMMNATDYEEYCS

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI49574537, Length=115, Percent_Identity=45.2173913043478, Blast_Score=117, Evalue=3e-27,
Organism=Homo sapiens, GI89057342, Length=115, Percent_Identity=44.3478260869565, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1789271, Length=123, Percent_Identity=57.7235772357724, Blast_Score=145, Evalue=6e-37,
Organism=Caenorhabditis elegans, GI17551294, Length=118, Percent_Identity=44.9152542372881, Blast_Score=123, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17507493, Length=116, Percent_Identity=43.1034482758621, Blast_Score=120, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6319272, Length=121, Percent_Identity=36.3636363636364, Blast_Score=96, Evalue=1e-21,
Organism=Drosophila melanogaster, GI17865652, Length=118, Percent_Identity=44.9152542372881, Blast_Score=111, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 13719; Mature: 13719

Theoretical pI: Translated: 3.56; Mature: 3.56

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHDVPENLLYSKDHEWIEIDGDIGTIGISDHAQAELSDVVFVDLPEVGATVAAGDPVAVV
CCCCCCCCEECCCCCEEEECCCEEEEECCCCCCCCCCCEEEEECCCCCCEEECCCCHHHH
ESVKAASDVYTPVSGEILEVNEELSNDPSLINSDPYGAGWLYKIRLDVPTETEDMMNATD
HHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCEEEEEEEECCCCHHHHHCCCC
YEEYCS
HHHHCC
>Mature Secondary Structure
MHDVPENLLYSKDHEWIEIDGDIGTIGISDHAQAELSDVVFVDLPEVGATVAAGDPVAVV
CCCCCCCCEECCCCCEEEECCCEEEEECCCCCCCCCCCEEEEECCCCCCEEECCCCHHHH
ESVKAASDVYTPVSGEILEVNEELSNDPSLINSDPYGAGWLYKIRLDVPTETEDMMNATD
HHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCEEEEEEEECCCCHHHHHCCCC
YEEYCS
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA