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The map label for this gene is glnS [H]

Identifier: 187734928

GI number: 187734928

Start: 504133

End: 505812

Strand: Direct

Name: glnS [H]

Synonym: Amuc_0421

Alternate gene names: 187734928

Gene position: 504133-505812 (Clockwise)

Preceding gene: 187734925

Following gene: 187734929

Centisome position: 18.92

GC content: 58.1

Gene sequence:

>1680_bases
ATGTCATTGCAGCCTGCCGAACGCCGAGATTTCATCCGCGATATGATTTCTGATGACCTTGCCTCCGGCAAGCATCAGGC
CCCGGTAACACGCTTTCCCCCGGAACCCAATGGGTATCTTCATATCGGACATGCCAAATCAATCTGCCTCAACTTCGGCA
TCGCCCAGGAATTTCCGGGTGCGCGCTGCCACCTGCGTTTTGACGACACCAACCCCAGCAAGGAAGACCAGGAATATGTG
GACAGCATTCAGGAAGACATCCGCTGGCTGGGCTTTGACTGGGGCGACAACCTTTTCTTTGCCAGCAACATGTTCGACTT
CTTTTACGAATGCGCCGTTTCCCTCATCCGCAAGGGATTGGCCTACGTAGACGAACAGACGGTGGAGGAAATACGCGCCC
AGCGCGGCAACGTGAACGTGCCCGGACAGGAATCACCCTACCGCAACCGCTCCGTGGAAGAAAACCTGGCACGCTTCCAG
GCCATGAAAAACGGGGAAATCCCGGAAGGCAGAGCCATCCTGCGGGCCAGGATAGACATGGCCTCCAGCAACATGAACAT
GCGGGACCCCGTGCTGTACCGCATCATGTTCGCGGAACACCACAACACGGGCAGTTCCTGGTGCATTTACCCCATGTATG
ACTTCGCCCACCCGCTGGAAGATGCCTACGAACACATCACCCATTCCCTCTGCACGCTGGAATTTGAAAACCACCGCCCC
CTCTATGACTGGGTCATTGAAAACTGCCCCGTCCCGGCACGCCCGCGCCAGACGGAATTCGCCCGCCTGAACCTTACCTA
CACCGTCATGAGCAAGCGCAAGCTGCTGCAACTGGTGCAGGAGGGACACGTCTCCGGCTGGGACGATCCACGGATGCCCA
CCGTCTCCGGCATGCGCCGCCGGGGCTACACTCCTGCCGCCATCCGCAACTTCTGCCACACCATAGGCATCACTAAATTC
AACGGCTTCACGGATGTGGCCCTGCTGGAATACAGCGTAAGGGAAGACCTGAATGCCAACGCGCCGCGCCGCATGGCCGT
GCTCAATCCCCTCAAGGTCACTATCACCACCCTGCCGGAAAACGCGGAAGAAATGGCTGAAGTGCTGAACAATCCGGAAA
AACCGGAAGAGGGCGTCCGCCGCCTCCCCATCACAAGGGAAGTCTGGATTGAACGGGACGACTTCATGCTGGACCCTCCT
AAAAAATACTTCCGTCTGGCCCCCGGCCGCACCGTACGCCTCCGGGGGGGCTACTGCATCACCTGCACGGACTACAAGCA
GGATGCGGAGGGAACTATCACGGAAATCTTCTGCGAACACATTCCCGGCACCATCGGCTCCAACCCTCCGGAAGGAATCC
AGTGCCGCGCCGCCATTCACTGGGTAAGCGCCAAATACGCGGTGGACGGAGAAATCCGCATCTATGACCGCCTTTTTACG
GAAGAAAACCCGGACGCTGCTGAAGAAGGGTTCCTGTCCGTCATGAATCCCTCTTCGCTGGCCGTCATCACAAACGCCAA
GCTGGAACCCTCCCTGGCCGGAGCGGAACCAGAATTCCGTTGCCAGTTCGAACGTCTCGGCTACTTTGTGGCGGACCGCA
GGGACCATGTACCGGGCCGTCGTCCTGTTTTCAACCGCACGGTGGCTCTCAAGGACTCCTGGGCAAAAAAACAAAAATAA

Upstream 100 bases:

>100_bases
GAAAAAAGATAAAAACGCTCTCCATGCTCCTCCTCCCGCCTCACGTCATTCCGAGTGAACACGCTTGCAGAAGAGGAAAT
TTGGTAGTAGAATTTCCGCC

Downstream 100 bases:

>100_bases
CCTCTCTTCATCCCCTTTTATGGACAATCAACTTCAATTCTGCACCGTTGAAGAAGCCGTGGAAGAAATACGGCAGGGAC
GGATGATCATCGTTACGGAC

Product: glutaminyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamine--tRNA ligase; GlnRS [H]

Number of amino acids: Translated: 559; Mature: 558

Protein sequence:

>559_residues
MSLQPAERRDFIRDMISDDLASGKHQAPVTRFPPEPNGYLHIGHAKSICLNFGIAQEFPGARCHLRFDDTNPSKEDQEYV
DSIQEDIRWLGFDWGDNLFFASNMFDFFYECAVSLIRKGLAYVDEQTVEEIRAQRGNVNVPGQESPYRNRSVEENLARFQ
AMKNGEIPEGRAILRARIDMASSNMNMRDPVLYRIMFAEHHNTGSSWCIYPMYDFAHPLEDAYEHITHSLCTLEFENHRP
LYDWVIENCPVPARPRQTEFARLNLTYTVMSKRKLLQLVQEGHVSGWDDPRMPTVSGMRRRGYTPAAIRNFCHTIGITKF
NGFTDVALLEYSVREDLNANAPRRMAVLNPLKVTITTLPENAEEMAEVLNNPEKPEEGVRRLPITREVWIERDDFMLDPP
KKYFRLAPGRTVRLRGGYCITCTDYKQDAEGTITEIFCEHIPGTIGSNPPEGIQCRAAIHWVSAKYAVDGEIRIYDRLFT
EENPDAAEEGFLSVMNPSSLAVITNAKLEPSLAGAEPEFRCQFERLGYFVADRRDHVPGRRPVFNRTVALKDSWAKKQK

Sequences:

>Translated_559_residues
MSLQPAERRDFIRDMISDDLASGKHQAPVTRFPPEPNGYLHIGHAKSICLNFGIAQEFPGARCHLRFDDTNPSKEDQEYV
DSIQEDIRWLGFDWGDNLFFASNMFDFFYECAVSLIRKGLAYVDEQTVEEIRAQRGNVNVPGQESPYRNRSVEENLARFQ
AMKNGEIPEGRAILRARIDMASSNMNMRDPVLYRIMFAEHHNTGSSWCIYPMYDFAHPLEDAYEHITHSLCTLEFENHRP
LYDWVIENCPVPARPRQTEFARLNLTYTVMSKRKLLQLVQEGHVSGWDDPRMPTVSGMRRRGYTPAAIRNFCHTIGITKF
NGFTDVALLEYSVREDLNANAPRRMAVLNPLKVTITTLPENAEEMAEVLNNPEKPEEGVRRLPITREVWIERDDFMLDPP
KKYFRLAPGRTVRLRGGYCITCTDYKQDAEGTITEIFCEHIPGTIGSNPPEGIQCRAAIHWVSAKYAVDGEIRIYDRLFT
EENPDAAEEGFLSVMNPSSLAVITNAKLEPSLAGAEPEFRCQFERLGYFVADRRDHVPGRRPVFNRTVALKDSWAKKQK
>Mature_558_residues
SLQPAERRDFIRDMISDDLASGKHQAPVTRFPPEPNGYLHIGHAKSICLNFGIAQEFPGARCHLRFDDTNPSKEDQEYVD
SIQEDIRWLGFDWGDNLFFASNMFDFFYECAVSLIRKGLAYVDEQTVEEIRAQRGNVNVPGQESPYRNRSVEENLARFQA
MKNGEIPEGRAILRARIDMASSNMNMRDPVLYRIMFAEHHNTGSSWCIYPMYDFAHPLEDAYEHITHSLCTLEFENHRPL
YDWVIENCPVPARPRQTEFARLNLTYTVMSKRKLLQLVQEGHVSGWDDPRMPTVSGMRRRGYTPAAIRNFCHTIGITKFN
GFTDVALLEYSVREDLNANAPRRMAVLNPLKVTITTLPENAEEMAEVLNNPEKPEEGVRRLPITREVWIERDDFMLDPPK
KYFRLAPGRTVRLRGGYCITCTDYKQDAEGTITEIFCEHIPGTIGSNPPEGIQCRAAIHWVSAKYAVDGEIRIYDRLFTE
ENPDAAEEGFLSVMNPSSLAVITNAKLEPSLAGAEPEFRCQFERLGYFVADRRDHVPGRRPVFNRTVALKDSWAKKQK

Specific function: Unknown

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI4826960, Length=531, Percent_Identity=44.6327683615819, Blast_Score=401, Evalue=1e-112,
Organism=Homo sapiens, GI62241042, Length=378, Percent_Identity=38.8888888888889, Blast_Score=244, Evalue=2e-64,
Organism=Escherichia coli, GI1786895, Length=556, Percent_Identity=57.9136690647482, Blast_Score=646, Evalue=0.0,
Organism=Escherichia coli, GI1788743, Length=310, Percent_Identity=23.5483870967742, Blast_Score=66, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17543332, Length=534, Percent_Identity=42.8838951310861, Blast_Score=398, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI17511007, Length=374, Percent_Identity=37.1657754010695, Blast_Score=223, Evalue=2e-58,
Organism=Saccharomyces cerevisiae, GI6324742, Length=564, Percent_Identity=39.8936170212766, Blast_Score=375, Evalue=1e-104,
Organism=Saccharomyces cerevisiae, GI6321192, Length=539, Percent_Identity=34.5083487940631, Blast_Score=273, Evalue=6e-74,
Organism=Drosophila melanogaster, GI17864488, Length=537, Percent_Identity=42.6443202979516, Blast_Score=410, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24649466, Length=418, Percent_Identity=34.4497607655502, Blast_Score=221, Evalue=1e-57,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR004514
- InterPro:   IPR022861
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR020059
- InterPro:   IPR020056
- InterPro:   IPR011035
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c; PF03950 tRNA-synt_1c_C [H]

EC number: =6.1.1.18 [H]

Molecular weight: Translated: 64111; Mature: 63980

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLQPAERRDFIRDMISDDLASGKHQAPVTRFPPEPNGYLHIGHAKSICLNFGIAQEFPG
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCHHHHHHCCCCHHCCCC
ARCHLRFDDTNPSKEDQEYVDSIQEDIRWLGFDWGDNLFFASNMFDFFYECAVSLIRKGL
CEEEEEECCCCCCCHHHHHHHHHHHHHHEECCCCCCCEEEHHHHHHHHHHHHHHHHHHHH
AYVDEQTVEEIRAQRGNVNVPGQESPYRNRSVEENLARFQAMKNGEIPEGRAILRARIDM
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
ASSNMNMRDPVLYRIMFAEHHNTGSSWCIYPMYDFAHPLEDAYEHITHSLCTLEFENHRP
HCCCCCCCCCCEEEEEEEECCCCCCCEEEEECHHHHCHHHHHHHHHHHHHEEEEECCCCC
LYDWVIENCPVPARPRQTEFARLNLTYTVMSKRKLLQLVQEGHVSGWDDPRMPTVSGMRR
HHHHHHHCCCCCCCCCCCCEEEEEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHH
RGYTPAAIRNFCHTIGITKFNGFTDVALLEYSVREDLNANAPRRMAVLNPLKVTITTLPE
CCCCHHHHHHHHHHHCCEEECCCCCHHHHHHHHHHCCCCCCCCEEEEECCEEEEEEECCC
NAEEMAEVLNNPEKPEEGVRRLPITREVWIERDDFMLDPPKKYFRLAPGRTVRLRGGYCI
CHHHHHHHHCCCCCCHHHHHHCCCCHHHEECCCCCEECCCHHHHEECCCCEEEECCCEEE
TCTDYKQDAEGTITEIFCEHIPGTIGSNPPEGIQCRAAIHWVSAKYAVDGEIRIYDRLFT
EECCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEEEEEEECCCEEEEEHHHC
EENPDAAEEGFLSVMNPSSLAVITNAKLEPSLAGAEPEFRCQFERLGYFVADRRDHVPGR
CCCCCHHHHCCEEEECCCCEEEEECCCCCCCCCCCCCHHEEEHHHHCCCEECCCCCCCCC
RPVFNRTVALKDSWAKKQK
CCCCCCEEEECCCHHCCCC
>Mature Secondary Structure 
SLQPAERRDFIRDMISDDLASGKHQAPVTRFPPEPNGYLHIGHAKSICLNFGIAQEFPG
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCHHHHHHCCCCHHCCCC
ARCHLRFDDTNPSKEDQEYVDSIQEDIRWLGFDWGDNLFFASNMFDFFYECAVSLIRKGL
CEEEEEECCCCCCCHHHHHHHHHHHHHHEECCCCCCCEEEHHHHHHHHHHHHHHHHHHHH
AYVDEQTVEEIRAQRGNVNVPGQESPYRNRSVEENLARFQAMKNGEIPEGRAILRARIDM
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
ASSNMNMRDPVLYRIMFAEHHNTGSSWCIYPMYDFAHPLEDAYEHITHSLCTLEFENHRP
HCCCCCCCCCCEEEEEEEECCCCCCCEEEEECHHHHCHHHHHHHHHHHHHEEEEECCCCC
LYDWVIENCPVPARPRQTEFARLNLTYTVMSKRKLLQLVQEGHVSGWDDPRMPTVSGMRR
HHHHHHHCCCCCCCCCCCCEEEEEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHH
RGYTPAAIRNFCHTIGITKFNGFTDVALLEYSVREDLNANAPRRMAVLNPLKVTITTLPE
CCCCHHHHHHHHHHHCCEEECCCCCHHHHHHHHHHCCCCCCCCEEEEECCEEEEEEECCC
NAEEMAEVLNNPEKPEEGVRRLPITREVWIERDDFMLDPPKKYFRLAPGRTVRLRGGYCI
CHHHHHHHHCCCCCCHHHHHHCCCCHHHEECCCCCEECCCHHHHEECCCCEEEECCCEEE
TCTDYKQDAEGTITEIFCEHIPGTIGSNPPEGIQCRAAIHWVSAKYAVDGEIRIYDRLFT
EECCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEEEEEEECCCEEEEEHHHC
EENPDAAEEGFLSVMNPSSLAVITNAKLEPSLAGAEPEFRCQFERLGYFVADRRDHVPGR
CCCCCHHHHCCEEEECCCCEEEEECCCCCCCCCCCCCHHEEEHHHHCCCEECCCCCCCCC
RPVFNRTVALKDSWAKKQK
CCCCCCEEEECCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]