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The map label for this gene is pnp

Identifier: 187734890

GI number: 187734890

Start: 457994

End: 460138

Strand: Reverse

Name: pnp

Synonym: Amuc_0382

Alternate gene names: 187734890

Gene position: 460138-457994 (Counterclockwise)

Preceding gene: 187734891

Following gene: 187734889

Centisome position: 17.27

GC content: 56.32

Gene sequence:

>2145_bases
ATGAGCATACATTCAGTTGAATGCAACGTTGGTACGAATCCTATCACGATTGAAACCGGCAAGATGGCCCGTTTGGCGGA
CGGTGCTGTTGTTGTCCGCAGCGGCGATACCGTCGTTCTTGTGACCGTGGTAAGCGCCACCAAAGTCAAGGAAGGGCAAA
CCTTCTTCCCCCTGTCCGTGGAGTACAAGGAAAAGGCCGCTGCGGCGGGTATGTTCCCCGGCGGCTATTTCAAACGCGAA
GGGCGTCCCACGGAAAAGGAAATCCTGACATGCCGCATGACGGACCGCCCCCTGCGCCCGATGTTCCCCAAGGGCTACTT
CTACGATACGCAGGTAATTACGCTTCTGCTCTCCGCCGATGGTGAAAACGAACCGGACATCCTGAGCATTAACGGCGCTT
CCGCCGCCTGTGTCGTTTCCGATCTTCCCTTTGCCGAACCCGTAGGGGCTGTGCGTGTGGGCCGTATTGACGGACAGTTT
GTTATCAATCCGACCAACTCCCAGCGTGAACATAGTCAGCTGGATCTGGTATTTGCCGGCACGAAGGATCAGGTCATCAT
GATTGAAGGTTCCGCTAATGAACTGCCGGAAGAAGATTTTATCGCCGCTTTGCGTGTCGCCCAGGAGAACGTGAAGGTCA
TTTGCGAAAAGCAGGAAGAGCTCCGCGCCGTTTGCGGTAAGGAGAAGCGCGCTTATGAACTTTGCCTTGCCAAGCCGGAA
TTGCTGGAAATCGGCTATGAAATTGCCGGCGATCGTATTGAGGAAGCCATTTACGCCCCTTCCAAGGTGGAGCGTCAGAA
AAAAGTGGGGGCCCTGCGTGACGAAGTGGAGGCCGCCATCAAGGAACGCCATCCGGAAGCTACTGACTTTGATGTGGAAC
AGGTTTTTGAATATATTCAGAAAAAGGCTTTCCGCATCTCCATCATGGAAAAGGACAAGCGGGCCGATGGCCGGGCTCTC
AAGCAGCTGCGCCCTCTGACTGCGGAAGTCAATGTGCTGCCTCCTGTGGTGCACGGTTCCGCGATGTTCGCCCGCGGTGA
AACGATGTCCCTTTGCCTGGCGACGCTCGCTCCGATGGAAGAACGCCAGTACATGGACAATTACACGGGCAGCGTGAATG
AAAAGCGTTTCATCCTGCACTACAATTTCCCGCCTTTCTCCGTGGGAGACACCGGCCGTTTCGGCGGTCAGAACCGCCGG
GAAATCGGCCACGGCGCTTTGGCGGAACGTTCCATCGCTCCGGTCGTGCCTGGCGAACAGGAATTCCCGTACGCCATTCG
CGTCTCTTCTGAAATCATGGAATCCAACGGTTCCACCTCCATGGCTTCCGTCTGTGCGGGCACGATGTCTCTGCTGGCGG
CCGGCGTGCCCCTCAAGCGCCCTGTAGCGGGTATTTCCGTGGGCCTGGTGACGGAACAGAATGACCAGCATGAAATCACC
TCTTACAAAACTCTGCTGGATATCATCGGTTCCGAAGACTTTTACGGTGATATGGACTTCAAGCTCTGCGGCACGTCGGA
AGGCGTGACAGGCTACCAGCTGGACCTCAAGCTGCCCGGCATTCCCCTCTCCATATTGGAGGAAGCCATTCACGTGGCGA
AAGCCGGCCGCACGGATGTTCTGAAGGTCATGAATGAAGCCATTGCAGCTCCGGCCCAGATGAGCCCGAATGCTCCCCGT
ATTGAGACCACCAAAATTCCCGCCGACCGCATTGGCGAACTGATCGGCCCCGGCGGCAAGAACATTAAGGCTATTCAGGC
CGAATCCGGCGCCGATATCAATATCGAGGAAGATGGCACCGTGCATATTTACGCCGCCAAGCAGGAAGGCCTGGACCGTG
CTCTGGAATTGGTCACCCGCATGTTCAAGACCATTGAAATCGGGGAACTGTACACCGGTAAGATTGTTTCCACGACCACC
TTTGGAGCGTTTATGGAAGTGCTGCCCGGCAAGGATGGCCTGATTCATATCTCCGAACTGGCCGAAGGCCGCACCGCCAA
GACGGAGGACGTCGTGAGCGTCGGAGACGTGGTGACCGCCAAGTGCATTGGTATTGACGACAAGGGACGCGTGAAAATGT
CCATACGTGCCGCTTTGCGAGATGCCAAGGCTGCCGAAGCAGAAGCCGCCGGCATTACGGAATAA

Upstream 100 bases:

>100_bases
CCTGAGGCACTGCCCGTACCTGACGCCATTCAGGCTTCCCGCCCGGAGCCGGCCTGGATGGGCTGATGAGGCTGCGGGTG
ATTGAAAGACAAAAAATATA

Downstream 100 bases:

>100_bases
CGTTTTTTGTTGAGCCTTTAAACTCTCAGCCCCGTTTCCGGTTATTCCGGGAACGGGGCTTTCCGGTTGCTGGAAAGATG
TTTTGCAGTTGCTTCAACTG

Product: Polyribonucleotide nucleotidyltransferase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 714; Mature: 713

Protein sequence:

>714_residues
MSIHSVECNVGTNPITIETGKMARLADGAVVVRSGDTVVLVTVVSATKVKEGQTFFPLSVEYKEKAAAAGMFPGGYFKRE
GRPTEKEILTCRMTDRPLRPMFPKGYFYDTQVITLLLSADGENEPDILSINGASAACVVSDLPFAEPVGAVRVGRIDGQF
VINPTNSQREHSQLDLVFAGTKDQVIMIEGSANELPEEDFIAALRVAQENVKVICEKQEELRAVCGKEKRAYELCLAKPE
LLEIGYEIAGDRIEEAIYAPSKVERQKKVGALRDEVEAAIKERHPEATDFDVEQVFEYIQKKAFRISIMEKDKRADGRAL
KQLRPLTAEVNVLPPVVHGSAMFARGETMSLCLATLAPMEERQYMDNYTGSVNEKRFILHYNFPPFSVGDTGRFGGQNRR
EIGHGALAERSIAPVVPGEQEFPYAIRVSSEIMESNGSTSMASVCAGTMSLLAAGVPLKRPVAGISVGLVTEQNDQHEIT
SYKTLLDIIGSEDFYGDMDFKLCGTSEGVTGYQLDLKLPGIPLSILEEAIHVAKAGRTDVLKVMNEAIAAPAQMSPNAPR
IETTKIPADRIGELIGPGGKNIKAIQAESGADINIEEDGTVHIYAAKQEGLDRALELVTRMFKTIEIGELYTGKIVSTTT
FGAFMEVLPGKDGLIHISELAEGRTAKTEDVVSVGDVVTAKCIGIDDKGRVKMSIRAALRDAKAAEAEAAGITE

Sequences:

>Translated_714_residues
MSIHSVECNVGTNPITIETGKMARLADGAVVVRSGDTVVLVTVVSATKVKEGQTFFPLSVEYKEKAAAAGMFPGGYFKRE
GRPTEKEILTCRMTDRPLRPMFPKGYFYDTQVITLLLSADGENEPDILSINGASAACVVSDLPFAEPVGAVRVGRIDGQF
VINPTNSQREHSQLDLVFAGTKDQVIMIEGSANELPEEDFIAALRVAQENVKVICEKQEELRAVCGKEKRAYELCLAKPE
LLEIGYEIAGDRIEEAIYAPSKVERQKKVGALRDEVEAAIKERHPEATDFDVEQVFEYIQKKAFRISIMEKDKRADGRAL
KQLRPLTAEVNVLPPVVHGSAMFARGETMSLCLATLAPMEERQYMDNYTGSVNEKRFILHYNFPPFSVGDTGRFGGQNRR
EIGHGALAERSIAPVVPGEQEFPYAIRVSSEIMESNGSTSMASVCAGTMSLLAAGVPLKRPVAGISVGLVTEQNDQHEIT
SYKTLLDIIGSEDFYGDMDFKLCGTSEGVTGYQLDLKLPGIPLSILEEAIHVAKAGRTDVLKVMNEAIAAPAQMSPNAPR
IETTKIPADRIGELIGPGGKNIKAIQAESGADINIEEDGTVHIYAAKQEGLDRALELVTRMFKTIEIGELYTGKIVSTTT
FGAFMEVLPGKDGLIHISELAEGRTAKTEDVVSVGDVVTAKCIGIDDKGRVKMSIRAALRDAKAAEAEAAGITE
>Mature_713_residues
SIHSVECNVGTNPITIETGKMARLADGAVVVRSGDTVVLVTVVSATKVKEGQTFFPLSVEYKEKAAAAGMFPGGYFKREG
RPTEKEILTCRMTDRPLRPMFPKGYFYDTQVITLLLSADGENEPDILSINGASAACVVSDLPFAEPVGAVRVGRIDGQFV
INPTNSQREHSQLDLVFAGTKDQVIMIEGSANELPEEDFIAALRVAQENVKVICEKQEELRAVCGKEKRAYELCLAKPEL
LEIGYEIAGDRIEEAIYAPSKVERQKKVGALRDEVEAAIKERHPEATDFDVEQVFEYIQKKAFRISIMEKDKRADGRALK
QLRPLTAEVNVLPPVVHGSAMFARGETMSLCLATLAPMEERQYMDNYTGSVNEKRFILHYNFPPFSVGDTGRFGGQNRRE
IGHGALAERSIAPVVPGEQEFPYAIRVSSEIMESNGSTSMASVCAGTMSLLAAGVPLKRPVAGISVGLVTEQNDQHEITS
YKTLLDIIGSEDFYGDMDFKLCGTSEGVTGYQLDLKLPGIPLSILEEAIHVAKAGRTDVLKVMNEAIAAPAQMSPNAPRI
ETTKIPADRIGELIGPGGKNIKAIQAESGADINIEEDGTVHIYAAKQEGLDRALELVTRMFKTIEIGELYTGKIVSTTTF
GAFMEVLPGKDGLIHISELAEGRTAKTEDVVSVGDVVTAKCIGIDDKGRVKMSIRAALRDAKAAEAEAAGITE

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=708, Percent_Identity=40.5367231638418, Blast_Score=546, Evalue=1e-155,
Organism=Escherichia coli, GI145693187, Length=689, Percent_Identity=41.944847605225, Blast_Score=546, Evalue=1e-156,
Organism=Caenorhabditis elegans, GI115534063, Length=712, Percent_Identity=33.7078651685393, Blast_Score=345, Evalue=4e-95,
Organism=Drosophila melanogaster, GI281362905, Length=703, Percent_Identity=37.8378378378378, Blast_Score=475, Evalue=1e-134,
Organism=Drosophila melanogaster, GI24651641, Length=703, Percent_Identity=37.8378378378378, Blast_Score=475, Evalue=1e-134,
Organism=Drosophila melanogaster, GI24651643, Length=703, Percent_Identity=37.8378378378378, Blast_Score=475, Evalue=1e-134,
Organism=Drosophila melanogaster, GI161079377, Length=638, Percent_Identity=36.833855799373, Blast_Score=424, Evalue=1e-119,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_AKKM8 (B2UNA9)

Other databases:

- EMBL:   CP001071
- RefSeq:   YP_001877002.1
- ProteinModelPortal:   B2UNA9
- GeneID:   6274858
- GenomeReviews:   CP001071_GR
- KEGG:   amu:Amuc_0382
- HOGENOM:   HBG382411
- OMA:   YGETVVL
- ProtClustDB:   CLSK2335772
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 77481; Mature: 77349

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIHSVECNVGTNPITIETGKMARLADGAVVVRSGDTVVLVTVVSATKVKEGQTFFPLSV
CCEEEEEECCCCCCEEEECCCCEEECCCEEEEECCCEEEEEEEEEHHEECCCCEEEEEEE
EYKEKAAAAGMFPGGYFKREGRPTEKEILTCRMTDRPLRPMFPKGYFYDTQVITLLLSAD
CHHHHHHHCCCCCCCCCCCCCCCCHHHEEEEEECCCCCCCCCCCCEEECCEEEEEEEECC
GENEPDILSINGASAACVVSDLPFAEPVGAVRVGRIDGQFVINPTNSQREHSQLDLVFAG
CCCCCCEEEECCCCEEEEEECCCCCCCCCCEEEEEECCEEEECCCCCCCCCCCEEEEEEC
TKDQVIMIEGSANELPEEDFIAALRVAQENVKVICEKQEELRAVCGKEKRAYELCLAKPE
CCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHEEECCHHHHHHHHCCCHHHHHHHCCCHH
LLEIGYEIAGDRIEEAIYAPSKVERQKKVGALRDEVEAAIKERHPEATDFDVEQVFEYIQ
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
KKAFRISIMEKDKRADGRALKQLRPLTAEVNVLPPVVHGSAMFARGETMSLCLATLAPME
HHEEEEEEEECCCCCCHHHHHHHCCCCEEEECCCCEECCCEEEECCCHHHHHHHHHCCHH
ERQYMDNYTGSVNEKRFILHYNFPPFSVGDTGRFGGQNRREIGHGALAERSIAPVVPGEQ
HHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHCCCCHHCCCCCCCCCCCC
EFPYAIRVSSEIMESNGSTSMASVCAGTMSLLAAGVPLKRPVAGISVGLVTEQNDQHEIT
CCCEEEEECHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCHHHH
SYKTLLDIIGSEDFYGDMDFKLCGTSEGVTGYQLDLKLPGIPLSILEEAIHVAKAGRTDV
HHHHHHHHHCCCCCCCCCCEEEECCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCHHH
LKVMNEAIAAPAQMSPNAPRIETTKIPADRIGELIGPGGKNIKAIQAESGADINIEEDGT
HHHHHHHHCCCCCCCCCCCCEEEECCCHHHHHHHHCCCCCCEEEEECCCCCEEEECCCCE
VHIYAAKQEGLDRALELVTRMFKTIEIGELYTGKIVSTTTFGAFMEVLPGKDGLIHISEL
EEEEECCHHHHHHHHHHHHHHHHHEECCCCCCCCEEEEHHHHHHHHHCCCCCCEEEHHHH
AEGRTAKTEDVVSVGDVVTAKCIGIDDKGRVKMSIRAALRDAKAAEAEAAGITE
CCCCCCCCHHHHHHCCEEEEEEECCCCCCCEEEEHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SIHSVECNVGTNPITIETGKMARLADGAVVVRSGDTVVLVTVVSATKVKEGQTFFPLSV
CEEEEEECCCCCCEEEECCCCEEECCCEEEEECCCEEEEEEEEEHHEECCCCEEEEEEE
EYKEKAAAAGMFPGGYFKREGRPTEKEILTCRMTDRPLRPMFPKGYFYDTQVITLLLSAD
CHHHHHHHCCCCCCCCCCCCCCCCHHHEEEEEECCCCCCCCCCCCEEECCEEEEEEEECC
GENEPDILSINGASAACVVSDLPFAEPVGAVRVGRIDGQFVINPTNSQREHSQLDLVFAG
CCCCCCEEEECCCCEEEEEECCCCCCCCCCEEEEEECCEEEECCCCCCCCCCCEEEEEEC
TKDQVIMIEGSANELPEEDFIAALRVAQENVKVICEKQEELRAVCGKEKRAYELCLAKPE
CCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHEEECCHHHHHHHHCCCHHHHHHHCCCHH
LLEIGYEIAGDRIEEAIYAPSKVERQKKVGALRDEVEAAIKERHPEATDFDVEQVFEYIQ
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
KKAFRISIMEKDKRADGRALKQLRPLTAEVNVLPPVVHGSAMFARGETMSLCLATLAPME
HHEEEEEEEECCCCCCHHHHHHHCCCCEEEECCCCEECCCEEEECCCHHHHHHHHHCCHH
ERQYMDNYTGSVNEKRFILHYNFPPFSVGDTGRFGGQNRREIGHGALAERSIAPVVPGEQ
HHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHCCCCHHCCCCCCCCCCCC
EFPYAIRVSSEIMESNGSTSMASVCAGTMSLLAAGVPLKRPVAGISVGLVTEQNDQHEIT
CCCEEEEECHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCHHHH
SYKTLLDIIGSEDFYGDMDFKLCGTSEGVTGYQLDLKLPGIPLSILEEAIHVAKAGRTDV
HHHHHHHHHCCCCCCCCCCEEEECCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCHHH
LKVMNEAIAAPAQMSPNAPRIETTKIPADRIGELIGPGGKNIKAIQAESGADINIEEDGT
HHHHHHHHCCCCCCCCCCCCEEEECCCHHHHHHHHCCCCCCEEEEECCCCCEEEECCCCE
VHIYAAKQEGLDRALELVTRMFKTIEIGELYTGKIVSTTTFGAFMEVLPGKDGLIHISEL
EEEEECCHHHHHHHHHHHHHHHHHEECCCCCCCCEEEEHHHHHHHHHCCCCCCEEEHHHH
AEGRTAKTEDVVSVGDVVTAKCIGIDDKGRVKMSIRAALRDAKAAEAEAAGITE
CCCCCCCCHHHHHHCCEEEEEEECCCCCCCEEEEHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA