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The map label for this gene is pyrD

Identifier: 187734883

GI number: 187734883

Start: 449414

End: 450460

Strand: Reverse

Name: pyrD

Synonym: Amuc_0375

Alternate gene names: 187734883

Gene position: 450460-449414 (Counterclockwise)

Preceding gene: 187734885

Following gene: 187734882

Centisome position: 16.91

GC content: 54.63

Gene sequence:

>1047_bases
GTGTCACCCGCCTTATATTCCGCCGCTAAAAGTGTATTATTCCAAATGAATCCGGAAACTGCCCACAAGGTGACCTTGTG
GGGGTTGCGCCTGGCAGAAAAGATGCGTGTACTACCTCTAGTAATGGGGGAAGTTCCTTCTGATCCTGTGGAAATCCTGG
GGATGAAATTCCCCAACCGGGTGGGACTTGCCGCCGGAATGGACAAGGAAGCGGATACGGTAAGCGCTTTTGGCCAGGCC
GGATTCGGTTTTGTGGAAGTGGGTACGCTGACGCCCCGGCCGCAACCGGGCAATGAAAAGCCCCGCCTGTTCCGGCTGAT
TCCCCAGAAAGCTATTATCAACCGGATGGGATTTAACAATGAAGGTATTGCCGCCGGAGTGGAAAACATTCGTTCCGCCA
CTCGTTTTCACGGTGTTCTGGGGGTCAATATCGGGAAAAATAAAATAACTCCCAATGAGGATGCCGCCCAGGATTACCTT
ACTTGTCTGCGTGCGGCATGGCCTGTGGCGGATTATATAGCCATTAATTTCTCTTCTCCCAATACGCCAGGCCTGCGCGA
CCTTCAGGCGGCGGAACCCGCTGCCCGTCTGCTGGCTTCCCTGAAATCGGAGCAATCCAACTTGGCTGCGGAAACGGGGC
GCCATGTGCCCATTTTTATGAAAGTGGCTCCTGATGTGACAGATGAGCATATTGCGGAGCTCAGCCGCGTTTTTCTGGAT
GAGGGACTGGATGGTCTTATTGCTACGAATACGACTCTTTCCCGCGTCGGGGTAGAGGCCAATCCTCGGCATGAAGAAGC
AGGCGGCCTGTCAGGCGCTCCTCTGACGGAACGGTCCACGGAAGTTATTGGAGCTTTTGCTTCCGAATTGAAAGGACGTA
TTCCGATCATTGGCGTTGGAGGCATTATGAACGGGGTGGACGCCGTCGCCAAAATCAAGGCTGGAGCCAGCCTGGTGCAG
CTTTATACCGGCTTCGTTTATCGCGGCCCGGACCTTATCCGGGAATGCGTGGAAGCCATGAAGGCCGAATGCCCTGTTCA
CCGCTGA

Upstream 100 bases:

>100_bases
CCTTCCTGTTCATGGCAAGGAATTGTACCGGATAAAATCATCCCGGCAAGACGCTTCCCATTCTTGACCCTAAAAGAGGG
TGTGATACACTCCCCGCATT

Downstream 100 bases:

>100_bases
TTTCCTTTATTAACCGCTTACAGGATCCATCCATGCCCGGTTCCTTCGTACACCTTCACAATCACACGGAATATTCGTTG
TTGGACGGCGCAGTCCATAT

Product: Dihydroorotate oxidase

Products: NA

Alternate protein names: DHOdehase; DHOD; DHODase; Dihydroorotate oxidase

Number of amino acids: Translated: 348; Mature: 347

Protein sequence:

>348_residues
MSPALYSAAKSVLFQMNPETAHKVTLWGLRLAEKMRVLPLVMGEVPSDPVEILGMKFPNRVGLAAGMDKEADTVSAFGQA
GFGFVEVGTLTPRPQPGNEKPRLFRLIPQKAIINRMGFNNEGIAAGVENIRSATRFHGVLGVNIGKNKITPNEDAAQDYL
TCLRAAWPVADYIAINFSSPNTPGLRDLQAAEPAARLLASLKSEQSNLAAETGRHVPIFMKVAPDVTDEHIAELSRVFLD
EGLDGLIATNTTLSRVGVEANPRHEEAGGLSGAPLTERSTEVIGAFASELKGRIPIIGVGGIMNGVDAVAKIKAGASLVQ
LYTGFVYRGPDLIRECVEAMKAECPVHR

Sequences:

>Translated_348_residues
MSPALYSAAKSVLFQMNPETAHKVTLWGLRLAEKMRVLPLVMGEVPSDPVEILGMKFPNRVGLAAGMDKEADTVSAFGQA
GFGFVEVGTLTPRPQPGNEKPRLFRLIPQKAIINRMGFNNEGIAAGVENIRSATRFHGVLGVNIGKNKITPNEDAAQDYL
TCLRAAWPVADYIAINFSSPNTPGLRDLQAAEPAARLLASLKSEQSNLAAETGRHVPIFMKVAPDVTDEHIAELSRVFLD
EGLDGLIATNTTLSRVGVEANPRHEEAGGLSGAPLTERSTEVIGAFASELKGRIPIIGVGGIMNGVDAVAKIKAGASLVQ
LYTGFVYRGPDLIRECVEAMKAECPVHR
>Mature_347_residues
SPALYSAAKSVLFQMNPETAHKVTLWGLRLAEKMRVLPLVMGEVPSDPVEILGMKFPNRVGLAAGMDKEADTVSAFGQAG
FGFVEVGTLTPRPQPGNEKPRLFRLIPQKAIINRMGFNNEGIAAGVENIRSATRFHGVLGVNIGKNKITPNEDAAQDYLT
CLRAAWPVADYIAINFSSPNTPGLRDLQAAEPAARLLASLKSEQSNLAAETGRHVPIFMKVAPDVTDEHIAELSRVFLDE
GLDGLIATNTTLSRVGVEANPRHEEAGGLSGAPLTERSTEVIGAFASELKGRIPIIGVGGIMNGVDAVAKIKAGASLVQL
YTGFVYRGPDLIRECVEAMKAECPVHR

Specific function: Pyrimidine biosynthesis; fourth step. [C]

COG id: COG0167

COG function: function code F; Dihydroorotate dehydrogenase

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily

Homologues:

Organism=Homo sapiens, GI45006951, Length=338, Percent_Identity=42.603550295858, Blast_Score=245, Evalue=5e-65,
Organism=Escherichia coli, GI1787177, Length=332, Percent_Identity=49.6987951807229, Blast_Score=334, Evalue=5e-93,
Organism=Caenorhabditis elegans, GI17509475, Length=347, Percent_Identity=38.9048991354467, Blast_Score=238, Evalue=3e-63,
Organism=Drosophila melanogaster, GI281361352, Length=331, Percent_Identity=44.7129909365559, Blast_Score=258, Evalue=5e-69,
Organism=Drosophila melanogaster, GI17137316, Length=331, Percent_Identity=44.7129909365559, Blast_Score=258, Evalue=5e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PYRD_AKKM8 (B2UNA2)

Other databases:

- EMBL:   CP001071
- RefSeq:   YP_001876995.1
- GeneID:   6274866
- GenomeReviews:   CP001071_GR
- KEGG:   amu:Amuc_0375
- HOGENOM:   HBG351027
- OMA:   SYVTVNI
- HAMAP:   MF_00225
- InterPro:   IPR013785
- InterPro:   IPR012135
- InterPro:   IPR005719
- InterPro:   IPR001295
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF000164
- TIGRFAMs:   TIGR01036

Pfam domain/function: PF01180 DHO_dh

EC number: =1.3.5.2

Molecular weight: Translated: 37231; Mature: 37100

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00911 DHODEHASE_1; PS00912 DHODEHASE_2

Important sites: ACT_SITE 179-179

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPALYSAAKSVLFQMNPETAHKVTLWGLRLAEKMRVLPLVMGEVPSDPVEILGMKFPNR
CCCHHHHHHHHHHEECCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCC
VGLAAGMDKEADTVSAFGQAGFGFVEVGTLTPRPQPGNEKPRLFRLIPQKAIINRMGFNN
CCHHCCCCCCHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCHHHHHCCHHHHHHHCCCCC
EGIAAGVENIRSATRFHGVLGVNIGKNKITPNEDAAQDYLTCLRAAWPVADYIAINFSSP
CCHHHHHHHHHHHHHHHCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEECCC
NTPGLRDLQAAEPAARLLASLKSEQSNLAAETGRHVPIFMKVAPDVTDEHIAELSRVFLD
CCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHH
EGLDGLIATNTTLSRVGVEANPRHEEAGGLSGAPLTERSTEVIGAFASELKGRIPIIGVG
CCCCCEEECCCHHHHCCCCCCCCCHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEECC
GIMNGVDAVAKIKAGASLVQLYTGFVYRGPDLIRECVEAMKAECPVHR
HHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SPALYSAAKSVLFQMNPETAHKVTLWGLRLAEKMRVLPLVMGEVPSDPVEILGMKFPNR
CCHHHHHHHHHHEECCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCC
VGLAAGMDKEADTVSAFGQAGFGFVEVGTLTPRPQPGNEKPRLFRLIPQKAIINRMGFNN
CCHHCCCCCCHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCHHHHHCCHHHHHHHCCCCC
EGIAAGVENIRSATRFHGVLGVNIGKNKITPNEDAAQDYLTCLRAAWPVADYIAINFSSP
CCHHHHHHHHHHHHHHHCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEECCC
NTPGLRDLQAAEPAARLLASLKSEQSNLAAETGRHVPIFMKVAPDVTDEHIAELSRVFLD
CCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHH
EGLDGLIATNTTLSRVGVEANPRHEEAGGLSGAPLTERSTEVIGAFASELKGRIPIIGVG
CCCCCEEECCCHHHHCCCCCCCCCHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEECC
GIMNGVDAVAKIKAGASLVQLYTGFVYRGPDLIRECVEAMKAECPVHR
HHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA