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The map label for this gene is def [H]

Identifier: 187734744

GI number: 187734744

Start: 290764

End: 291444

Strand: Direct

Name: def [H]

Synonym: Amuc_0232

Alternate gene names: 187734744

Gene position: 290764-291444 (Clockwise)

Preceding gene: 187734741

Following gene: 187734746

Centisome position: 10.91

GC content: 55.65

Gene sequence:

>681_bases
ATGTCACACTCTTTTATCCATATCATCCTGTCTGCCGTGATTCTTGATATTCTTCAATACGGACATCCTCTTTTGAGAGA
GGAATGCGGGCCCGTGGTTCATATCAACAGGGATATCCTTTCCTTCCTGGACGATATGCAGGAAACCCTGGCCCAGGGAG
GCATAGGCCTGGCCGCGCCGCAGGTGGGCAGGCCCATCCAGCTGGTCACCATCAACATCCCTTCCACAGACGCAACCACT
ACCTGGCTGGAAGTGGACGGATGCCCAACTACGTTGTCCCGAATCATGCCCTTGAATTTTATCAATCCCATCCTGCATCC
CTTCGGCAAAAAAGTGCCGTACCGGGAAGGGTGCCTGAGCATCACCAAAGTATATGCGAACGTGATGCGCCGCTCCTGCG
TAAGGGCTGTTTTAACCATGATGGACGGACGGACTGTGACCGTGAAATGCAACGGACTGCTGGCGCGCTGCCTGCAGCAC
GAGGTAGACCACCTTCACGGCGGCCTGTTTACGGATCTGGTTTCCTCCGGTGACCATGACAAGGTGATACGGAGGCTCAG
ATTGACCCATCCGGACGCTTTTGAAGAAGATGACGACAGCTACGCTCGCCGCATGAAGGAAAAACGCCGCGCCCAGGCAC
GGAAAGCCCGGATGCCGCAGCCTCCCGGAAAAACAGCCTGA

Upstream 100 bases:

>100_bases
TCAAAGTTCAAGGCCTTTTCATTTCATCCGGGAAAAACATTTCCGTTCCTTCGACGGAAAAAATATTCATTTAATATAAT
GAACACTCTTCTGAATCGGG

Downstream 100 bases:

>100_bases
CGTTCAGGCAACAAACCTATCCCCCCTTTTACTGCCCTTCCCTAAAAAACGCCCCGGTCTCCCTCCAGGAAACCGGGGCA
TCCATAATCACATGGAAAGA

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase [H]

Number of amino acids: Translated: 226; Mature: 225

Protein sequence:

>226_residues
MSHSFIHIILSAVILDILQYGHPLLREECGPVVHINRDILSFLDDMQETLAQGGIGLAAPQVGRPIQLVTINIPSTDATT
TWLEVDGCPTTLSRIMPLNFINPILHPFGKKVPYREGCLSITKVYANVMRRSCVRAVLTMMDGRTVTVKCNGLLARCLQH
EVDHLHGGLFTDLVSSGDHDKVIRRLRLTHPDAFEEDDDSYARRMKEKRRAQARKARMPQPPGKTA

Sequences:

>Translated_226_residues
MSHSFIHIILSAVILDILQYGHPLLREECGPVVHINRDILSFLDDMQETLAQGGIGLAAPQVGRPIQLVTINIPSTDATT
TWLEVDGCPTTLSRIMPLNFINPILHPFGKKVPYREGCLSITKVYANVMRRSCVRAVLTMMDGRTVTVKCNGLLARCLQH
EVDHLHGGLFTDLVSSGDHDKVIRRLRLTHPDAFEEDDDSYARRMKEKRRAQARKARMPQPPGKTA
>Mature_225_residues
SHSFIHIILSAVILDILQYGHPLLREECGPVVHINRDILSFLDDMQETLAQGGIGLAAPQVGRPIQLVTINIPSTDATTT
WLEVDGCPTTLSRIMPLNFINPILHPFGKKVPYREGCLSITKVYANVMRRSCVRAVLTMMDGRTVTVKCNGLLARCLQHE
VDHLHGGLFTDLVSSGDHDKVIRRLRLTHPDAFEEDDDSYARRMKEKRRAQARKARMPQPPGKTA

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Escherichia coli, GI1789682, Length=163, Percent_Identity=34.9693251533742, Blast_Score=81, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 25297; Mature: 25166

Theoretical pI: Translated: 8.61; Mature: 8.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHSFIHIILSAVILDILQYGHPLLREECGPVVHINRDILSFLDDMQETLAQGGIGLAAP
CCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCEEEECHHHHHHHHHHHHHHHHCCCCCCCC
QVGRPIQLVTINIPSTDATTTWLEVDGCPTTLSRIMPLNFINPILHPFGKKVPYREGCLS
CCCCCEEEEEEECCCCCCCEEEEEECCCHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHH
ITKVYANVMRRSCVRAVLTMMDGRTVTVKCNGLLARCLQHEVDHLHGGLFTDLVSSGDHD
HHHHHHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHCHHHHHHHCCCCHH
KVIRRLRLTHPDAFEEDDDSYARRMKEKRRAQARKARMPQPPGKTA
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SHSFIHIILSAVILDILQYGHPLLREECGPVVHINRDILSFLDDMQETLAQGGIGLAAP
CCHHHHHHHHHHHHHHHHHCCHHHHHHCCCEEEECHHHHHHHHHHHHHHHHCCCCCCCC
QVGRPIQLVTINIPSTDATTTWLEVDGCPTTLSRIMPLNFINPILHPFGKKVPYREGCLS
CCCCCEEEEEEECCCCCCCEEEEEECCCHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHH
ITKVYANVMRRSCVRAVLTMMDGRTVTVKCNGLLARCLQHEVDHLHGGLFTDLVSSGDHD
HHHHHHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHCHHHHHHHCCCCHH
KVIRRLRLTHPDAFEEDDDSYARRMKEKRRAQARKARMPQPPGKTA
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA