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The map label for this gene is birA [H]

Identifier: 187734718

GI number: 187734718

Start: 254809

End: 255588

Strand: Direct

Name: birA [H]

Synonym: Amuc_0205

Alternate gene names: 187734718

Gene position: 254809-255588 (Clockwise)

Preceding gene: 187734717

Following gene: 187734719

Centisome position: 9.56

GC content: 61.67

Gene sequence:

>780_bases
ATGAATTCCGCAGCCTCATTTTCCACCCCTCCGGAGGAGGAAGGACCATGCGGCGACTGGACTTATCATGAATGGGCTGA
GGCTTTTTCCACCAATGATCTGGCCCGCACCCTCCCCCCGGGGCATATTGCCGTCTGCCGCGTTCAGACAGGAGGCCGCG
GCAGGTTCAACCGCAAATGGATAGGGGAGGAAGGCGGCCTGTGGGCATCCTTCACCGTCCCCCTGTCCGCTCCTGAGGAT
ACAGCGGCCGGCGTGCACTGGGGCCATTTGCCCCTGGTTGCGGGGCTGGCCCTGTTCAACATGCTCCGCGGCATGGGGCT
GGATTCCGTGCGCCTGCGCTGGCCGAACGACCTGCTGGCGGGCAAATCCAAACTGGCGGGAATACTGGTGGAACGCCCGG
CGGAGAACATGGCCGTCATCGGCATCGGCATCAATGTTTTCAATGATGTATCCTCCATCGCCGGGGAAATCAAGGACCCT
CCGGCAAGGCTGGCGGATCTTGTTCGGGACTGCCCTTCCCTGCACGCCGTCATGGCTTCCCTGGGCAGTCATTTGAACCA
GGTTCTTTCCGTCTTTTCCCACGGCGGCGTCCCCGCCCTTCAGGAGGGTCTGTCCCGGGCATGGGCCGGAGAAAGGCCGG
TTTCCATTCTTACGGATGACGAAACCGTCCGGGGCATTTTTACAGGGATTGACGATCAGGGCAATCCTGTGCTATCCCTT
CCGGCCGGCACCGTCCGCACCATTCCGGCGCACCTGATCAACAGGCTCCTCGAAGAATGA

Upstream 100 bases:

>100_bases
GTTGCAGGCGTGATCGGCACCGCAGTCATCGCGGGCTACTACATCGCCACTCTGGCGAAATAACCAGAATTGAAAATTAC
TCCCGGCCTCGGCCCGTCCC

Downstream 100 bases:

>100_bases
GCCTGCCGCTCCGTTTTTACACGGGGTTTCTTTCTTTTTTCATCCAACAAAAATCACTGACATGAGTTACAAGGTACTGA
CACCAGAAGAAGCAGCAAGC

Product: biotin/acetyl-CoA-carboxylase ligase

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MNSAASFSTPPEEEGPCGDWTYHEWAEAFSTNDLARTLPPGHIAVCRVQTGGRGRFNRKWIGEEGGLWASFTVPLSAPED
TAAGVHWGHLPLVAGLALFNMLRGMGLDSVRLRWPNDLLAGKSKLAGILVERPAENMAVIGIGINVFNDVSSIAGEIKDP
PARLADLVRDCPSLHAVMASLGSHLNQVLSVFSHGGVPALQEGLSRAWAGERPVSILTDDETVRGIFTGIDDQGNPVLSL
PAGTVRTIPAHLINRLLEE

Sequences:

>Translated_259_residues
MNSAASFSTPPEEEGPCGDWTYHEWAEAFSTNDLARTLPPGHIAVCRVQTGGRGRFNRKWIGEEGGLWASFTVPLSAPED
TAAGVHWGHLPLVAGLALFNMLRGMGLDSVRLRWPNDLLAGKSKLAGILVERPAENMAVIGIGINVFNDVSSIAGEIKDP
PARLADLVRDCPSLHAVMASLGSHLNQVLSVFSHGGVPALQEGLSRAWAGERPVSILTDDETVRGIFTGIDDQGNPVLSL
PAGTVRTIPAHLINRLLEE
>Mature_259_residues
MNSAASFSTPPEEEGPCGDWTYHEWAEAFSTNDLARTLPPGHIAVCRVQTGGRGRFNRKWIGEEGGLWASFTVPLSAPED
TAAGVHWGHLPLVAGLALFNMLRGMGLDSVRLRWPNDLLAGKSKLAGILVERPAENMAVIGIGINVFNDVSSIAGEIKDP
PARLADLVRDCPSLHAVMASLGSHLNQVLSVFSHGGVPALQEGLSRAWAGERPVSILTDDETVRGIFTGIDDQGNPVLSL
PAGTVRTIPAHLINRLLEE

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=209, Percent_Identity=32.5358851674641, Blast_Score=73, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004408
- InterPro:   IPR004409
- InterPro:   IPR003142
- InterPro:   IPR004143
- InterPro:   IPR008988 [H]

Pfam domain/function: PF02237 BPL_C; PF03099 BPL_LipA_LipB [H]

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 27677; Mature: 27677

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSAASFSTPPEEEGPCGDWTYHEWAEAFSTNDLARTLPPGHIAVCRVQTGGRGRFNRKW
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHCCCCCEEEEEEECCCCCCCCCCE
IGEEGGLWASFTVPLSAPEDTAAGVHWGHLPLVAGLALFNMLRGMGLDSVRLRWPNDLLA
ECCCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHH
GKSKLAGILVERPAENMAVIGIGINVFNDVSSIAGEIKDPPARLADLVRDCPSLHAVMAS
CHHHHHHHHEECCCCCEEEEEECHHHHHHHHHHHHCCCCCHHHHHHHHHHCHHHHHHHHH
LGSHLNQVLSVFSHGGVPALQEGLSRAWAGERPVSILTDDETVRGIFTGIDDQGNPVLSL
HHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEECCHHHHHHHCCCCCCCCEEEEC
PAGTVRTIPAHLINRLLEE
CCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNSAASFSTPPEEEGPCGDWTYHEWAEAFSTNDLARTLPPGHIAVCRVQTGGRGRFNRKW
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHCCCCCEEEEEEECCCCCCCCCCE
IGEEGGLWASFTVPLSAPEDTAAGVHWGHLPLVAGLALFNMLRGMGLDSVRLRWPNDLLA
ECCCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHH
GKSKLAGILVERPAENMAVIGIGINVFNDVSSIAGEIKDPPARLADLVRDCPSLHAVMAS
CHHHHHHHHEECCCCCEEEEEECHHHHHHHHHHHHCCCCCHHHHHHHHHHCHHHHHHHHH
LGSHLNQVLSVFSHGGVPALQEGLSRAWAGERPVSILTDDETVRGIFTGIDDQGNPVLSL
HHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEECCHHHHHHHCCCCCCCCEEEEC
PAGTVRTIPAHLINRLLEE
CCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]