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The map label for this gene is mrcA [H]

Identifier: 187734690

GI number: 187734690

Start: 222775

End: 225435

Strand: Direct

Name: mrcA [H]

Synonym: Amuc_0177

Alternate gene names: 187734690

Gene position: 222775-225435 (Clockwise)

Preceding gene: 187734687

Following gene: 187734691

Centisome position: 8.36

GC content: 58.51

Gene sequence:

>2661_bases
ATGGGTTTAAACGACAAATCAAGGAAACGAAATGCCGCCTCCTGGAGGGAACGCATCCAAGATGGAGAAATGTCTTCCCG
TTCCCAGACGGAACGCAAAAAAAGGCCCCTTCCCCGTCCATCCTATCCCGGGTTGCCGCAGGAAGCTCCTGATCCGGCTG
TCATTCCCGGCCCTACCCGCAGAACGGGGAATTCCCGCAACGGTCCTTCCCGGTATGACACGCCGCCCCAGCGACGGACC
TCCCGCCCGTCTCCGAAGGAAGAGTACGAAGGCAATCCGGAAAGAGAAGAAGAAAACCGCCCCGCACGCGCAGGAACCAT
GCGCAGGCCGCGTTTCACGATTTTCGGAGCCCTGCTGTTTGTCCTGCTGATGCCGTTCCGCTTCATCGGCTCCCTGACGC
GGAATATCCGCTGGTTCATTTGCTGGCCCCTGCGCATCCTTCTGGGATGCTGCTTCGTCGGCATTGTGATCGGGGCCATC
CTGGTGTTTCTTTACGGCACCATTTCCAACCGCTATGACATTTCCGAGGTAAAGAGCAACATCCCGGAACGCACGGTGAT
TCTGGACCGTAAAAACCGGACCATCGGAACCCTGCACGGGGAAAACCGCAAGCGGGTATCCCTTCAGGAAGTTCCTCCCG
TTTTTATTGATGCCCTGCTCCTGCGCGAAGACAACCGCTTTTACGACCATGGCGGCGTGGACTGGATAGGCGTGGGCCGC
GCCTTTGCGCAGGTGCTCAAGCACAAGAGAGCTACGCAGGGGGCTTCCACCCTGACGATGCAGCTTGCTAAAATTACATA
TAACCACCGGGAGCGCAACCTCCATTCCAAACTGACGGAAGTGGCCCTGGCGAAACGCATTGAAGCCACTTATACGAAGG
ACGAAATTCTGGAGACTTACATCAACCGTATTTTCTGGGGGCACACCTTCCTGGGGATAGCGGCGGCGGCGCGGGGATAT
TACGACAAGGAACCCCGGGATTTATCCCTGGCGGAATGCGCCACCCTGGCCGGCATTATTTACGGCCCAAATGATTTTTC
CCCCATCAAACATCCGGAGGAAGCAAAAAAAGTGCGCGATATTGTCCTTGGCCTGCTGAAAAACGAAGGCAAGATTACAG
AAGTGCAGTATCAGGCGGCCCTGGCGGAGCCTATCGTCACCCGGACGCCCCAGTCCCGGTCTGAGGAAAATTACGCCATG
GGTCTCGTGCGCCGGGAGCTGGACGCCATTCTGGAGGAAGAAGACATCCGCCTGGGAGGGCTGGTGGTGCATACCACATT
GGACCTTGATCTGCAGAACGCCACGCTGGACGCCATCAATAAGCACATGGACGCGCTGGAAGCCCGCAAGGATTTCAAGA
AGCACCTGGCTTCCCTGCAGGCCAAGAGGGAGAAGAGGGGAATTCTGAAAAACAAGCTGACCACCAAGGCGGAATATGAG
GCCTCCCTGGCCGCCTGGAAGGCGCTCCCTGCGGAACAGAAGGAAAAGACGCAGCCGCCGATGCCCAATTACATCCAATC
CGCCGCCGTAGTCATGGACAATGCCACGGGGGCTCTTCTGGCCGTCGTCGGCGGCCGGGACGCGGAAGAATCCAAGCTGA
ACCGCGCCATTCAGTCCAGGCGGCAGACCGGCTCCCTGTTCAAACCTTTCATTTACGCCACGTTTTTCCAGCAGGGCAAT
TCTGCGGATACCCGCATTTCGGATGACAGGATTGCCTACGGGGAAATACGCGGAGCCGCCAACTGGTCCCCCCGCAATTC
GGACGGAACGTACCGAGGCATGAAGCCGGCCTCCTTCGGCCTCATCCTTTCCCGCAACACCATGTCCGTGCGCATCGGCA
ACCGCGCCGGTTTGTCCAATGTCATTCAATCCGCACAACTGGCGGGCTTCCACGGAAATATTTCCCGCACCCCGGCCCTT
TATCTGGGCACGTGGGAGGCGTCCCCTCTGGACATTGCCAGCGCCTACAGCGTTTTTGCCAACGGGGGCGTACGCCCCAC
ACCGTACATCATTGACCATATTACGGATTCCCAGGGCCAGCCGCGCTTCGCCATCACCAAGAGCAAGCGCACCGTCTATT
CCCAGCGGGCGGCGAACATTACCTCTTCCATCCTCCAGCAGGTCTGCAAGCCCGGAGGCACGGCAGGCAAAATTACCACG
CTGGGCTTTAAATCCCCCTGCGGCGGCAAAACTGGAACCACGAATAATTATACGAACGCATGGTTCGCCGGATATACGTC
CAACCTCACCTGCAGTGTCTGGGTGGGGTTTGATTCCTCCACCAAAATTCTGGAAAAAGGCTACGGAGGCACCCTGGCCC
TGCCCGTCTGGGTGGACATTATGCTTGCCGCCCAGAAGGAAGGCTATCCCGCCAACGCCATCCGTACGCGTCCCGGTTCG
GAAGGGCAGGCCGTACTCGTCTGCCGGGAATCCAATCAACTGGCCCATTCCGGCTGCCAGTACGCCAAGACTGCGTATTT
TGAAACCTCCGCAGGCTACCAGGCCCCCGCCAACATGTGTGAACAGCACATTCCCATGGCGGAACCGGATTCGGAAGAGA
GCATTCCCTATGCGGAACCGCTGGACGGCAGCGACGATAACATCCCCCTGGCCGAACCCGTGGAAGAAACGGACGGCATC
CCCTACGCCACCCCCATCTGA

Upstream 100 bases:

>100_bases
AAACGTTCAAAACGGCAGAATTTTGTCTATGGATTGCGACATGGCGCCCCATCTTTTGCTTAGACCATTTCGGTTATTTA
TGATAAACTCCGGCCCACGG

Downstream 100 bases:

>100_bases
TTGCTTATTTTTTCCTTATGATTTCCCCATTTTTTCCCTGGTTCCTGGCCTTTTTGCTGCTGATCGCCTGCGTGGCGCTG
CTGGTTCTGCTGCTGCGGTA

Product: Peptidoglycan glycosyltransferase

Products: NA

Alternate protein names: PBP-1a; PBP1a; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]

Number of amino acids: Translated: 886; Mature: 885

Protein sequence:

>886_residues
MGLNDKSRKRNAASWRERIQDGEMSSRSQTERKKRPLPRPSYPGLPQEAPDPAVIPGPTRRTGNSRNGPSRYDTPPQRRT
SRPSPKEEYEGNPEREEENRPARAGTMRRPRFTIFGALLFVLLMPFRFIGSLTRNIRWFICWPLRILLGCCFVGIVIGAI
LVFLYGTISNRYDISEVKSNIPERTVILDRKNRTIGTLHGENRKRVSLQEVPPVFIDALLLREDNRFYDHGGVDWIGVGR
AFAQVLKHKRATQGASTLTMQLAKITYNHRERNLHSKLTEVALAKRIEATYTKDEILETYINRIFWGHTFLGIAAAARGY
YDKEPRDLSLAECATLAGIIYGPNDFSPIKHPEEAKKVRDIVLGLLKNEGKITEVQYQAALAEPIVTRTPQSRSEENYAM
GLVRRELDAILEEEDIRLGGLVVHTTLDLDLQNATLDAINKHMDALEARKDFKKHLASLQAKREKRGILKNKLTTKAEYE
ASLAAWKALPAEQKEKTQPPMPNYIQSAAVVMDNATGALLAVVGGRDAEESKLNRAIQSRRQTGSLFKPFIYATFFQQGN
SADTRISDDRIAYGEIRGAANWSPRNSDGTYRGMKPASFGLILSRNTMSVRIGNRAGLSNVIQSAQLAGFHGNISRTPAL
YLGTWEASPLDIASAYSVFANGGVRPTPYIIDHITDSQGQPRFAITKSKRTVYSQRAANITSSILQQVCKPGGTAGKITT
LGFKSPCGGKTGTTNNYTNAWFAGYTSNLTCSVWVGFDSSTKILEKGYGGTLALPVWVDIMLAAQKEGYPANAIRTRPGS
EGQAVLVCRESNQLAHSGCQYAKTAYFETSAGYQAPANMCEQHIPMAEPDSEESIPYAEPLDGSDDNIPLAEPVEETDGI
PYATPI

Sequences:

>Translated_886_residues
MGLNDKSRKRNAASWRERIQDGEMSSRSQTERKKRPLPRPSYPGLPQEAPDPAVIPGPTRRTGNSRNGPSRYDTPPQRRT
SRPSPKEEYEGNPEREEENRPARAGTMRRPRFTIFGALLFVLLMPFRFIGSLTRNIRWFICWPLRILLGCCFVGIVIGAI
LVFLYGTISNRYDISEVKSNIPERTVILDRKNRTIGTLHGENRKRVSLQEVPPVFIDALLLREDNRFYDHGGVDWIGVGR
AFAQVLKHKRATQGASTLTMQLAKITYNHRERNLHSKLTEVALAKRIEATYTKDEILETYINRIFWGHTFLGIAAAARGY
YDKEPRDLSLAECATLAGIIYGPNDFSPIKHPEEAKKVRDIVLGLLKNEGKITEVQYQAALAEPIVTRTPQSRSEENYAM
GLVRRELDAILEEEDIRLGGLVVHTTLDLDLQNATLDAINKHMDALEARKDFKKHLASLQAKREKRGILKNKLTTKAEYE
ASLAAWKALPAEQKEKTQPPMPNYIQSAAVVMDNATGALLAVVGGRDAEESKLNRAIQSRRQTGSLFKPFIYATFFQQGN
SADTRISDDRIAYGEIRGAANWSPRNSDGTYRGMKPASFGLILSRNTMSVRIGNRAGLSNVIQSAQLAGFHGNISRTPAL
YLGTWEASPLDIASAYSVFANGGVRPTPYIIDHITDSQGQPRFAITKSKRTVYSQRAANITSSILQQVCKPGGTAGKITT
LGFKSPCGGKTGTTNNYTNAWFAGYTSNLTCSVWVGFDSSTKILEKGYGGTLALPVWVDIMLAAQKEGYPANAIRTRPGS
EGQAVLVCRESNQLAHSGCQYAKTAYFETSAGYQAPANMCEQHIPMAEPDSEESIPYAEPLDGSDDNIPLAEPVEETDGI
PYATPI
>Mature_885_residues
GLNDKSRKRNAASWRERIQDGEMSSRSQTERKKRPLPRPSYPGLPQEAPDPAVIPGPTRRTGNSRNGPSRYDTPPQRRTS
RPSPKEEYEGNPEREEENRPARAGTMRRPRFTIFGALLFVLLMPFRFIGSLTRNIRWFICWPLRILLGCCFVGIVIGAIL
VFLYGTISNRYDISEVKSNIPERTVILDRKNRTIGTLHGENRKRVSLQEVPPVFIDALLLREDNRFYDHGGVDWIGVGRA
FAQVLKHKRATQGASTLTMQLAKITYNHRERNLHSKLTEVALAKRIEATYTKDEILETYINRIFWGHTFLGIAAAARGYY
DKEPRDLSLAECATLAGIIYGPNDFSPIKHPEEAKKVRDIVLGLLKNEGKITEVQYQAALAEPIVTRTPQSRSEENYAMG
LVRRELDAILEEEDIRLGGLVVHTTLDLDLQNATLDAINKHMDALEARKDFKKHLASLQAKREKRGILKNKLTTKAEYEA
SLAAWKALPAEQKEKTQPPMPNYIQSAAVVMDNATGALLAVVGGRDAEESKLNRAIQSRRQTGSLFKPFIYATFFQQGNS
ADTRISDDRIAYGEIRGAANWSPRNSDGTYRGMKPASFGLILSRNTMSVRIGNRAGLSNVIQSAQLAGFHGNISRTPALY
LGTWEASPLDIASAYSVFANGGVRPTPYIIDHITDSQGQPRFAITKSKRTVYSQRAANITSSILQQVCKPGGTAGKITTL
GFKSPCGGKTGTTNNYTNAWFAGYTSNLTCSVWVGFDSSTKILEKGYGGTLALPVWVDIMLAAQKEGYPANAIRTRPGSE
GQAVLVCRESNQLAHSGCQYAKTAYFETSAGYQAPANMCEQHIPMAEPDSEESIPYAEPLDGSDDNIPLAEPVEETDGIP
YATPI

Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=597, Percent_Identity=30.4857621440536, Blast_Score=204, Evalue=3e-53,
Organism=Escherichia coli, GI87082258, Length=326, Percent_Identity=32.8220858895706, Blast_Score=155, Evalue=9e-39,
Organism=Escherichia coli, GI1788867, Length=237, Percent_Identity=30.8016877637131, Blast_Score=111, Evalue=2e-25,
Organism=Escherichia coli, GI1789601, Length=171, Percent_Identity=29.8245614035088, Blast_Score=74, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.1.129; 3.4.-.- [C]

Molecular weight: Translated: 98235; Mature: 98103

Theoretical pI: Translated: 9.57; Mature: 9.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLNDKSRKRNAASWRERIQDGEMSSRSQTERKKRPLPRPSYPGLPQEAPDPAVIPGPTR
CCCCCCHHHCHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
RTGNSRNGPSRYDTPPQRRTSRPSPKEEYEGNPEREEENRPARAGTMRRPRFTIFGALLF
CCCCCCCCCCCCCCCCHHCCCCCCCHHHCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHH
VLLMPFRFIGSLTRNIRWFICWPLRILLGCCFVGIVIGAILVFLYGTISNRYDISEVKSN
HHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHC
IPERTVILDRKNRTIGTLHGENRKRVSLQEVPPVFIDALLLREDNRFYDHGGVDWIGVGR
CCCEEEEEECCCCEEEEEECCCCCEEEHHHCCHHHHHHHHHHCCCCEECCCCCCEEHHHH
AFAQVLKHKRATQGASTLTMQLAKITYNHRERNLHSKLTEVALAKRIEATYTKDEILETY
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
INRIFWGHTFLGIAAAARGYYDKEPRDLSLAECATLAGIIYGPNDFSPIKHPEEAKKVRD
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHEEECCCCCCCCCCCHHHHHHHH
IVLGLLKNEGKITEVQYQAALAEPIVTRTPQSRSEENYAMGLVRRELDAILEEEDIRLGG
HHHHHHHCCCCEEEHHHHHHHHCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEECC
LVVHTTLDLDLQNATLDAINKHMDALEARKDFKKHLASLQAKREKRGILKNKLTTKAEYE
EEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
ASLAAWKALPAEQKEKTQPPMPNYIQSAAVVMDNATGALLAVVGGRDAEESKLNRAIQSR
HHHHHHHCCCCCHHHCCCCCCCHHHHHHHHEEECCCCEEEEEECCCCCHHHHHHHHHHHH
RQTGSLFKPFIYATFFQQGNSADTRISDDRIAYGEIRGAANWSPRNSDGTYRGMKPASFG
HHCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEHHCCCCCCCCCCCCCCCCCCCCCCCCE
LILSRNTMSVRIGNRAGLSNVIQSAQLAGFHGNISRTPALYLGTWEASPLDIASAYSVFA
EEEECCEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHH
NGGVRPTPYIIDHITDSQGQPRFAITKSKRTVYSQRAANITSSILQQVCKPGGTAGKITT
CCCCCCCCEEEEECCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
LGFKSPCGGKTGTTNNYTNAWFAGYTSNLTCSVWVGFDSSTKILEKGYGGTLALPVWVDI
ECCCCCCCCCCCCCCCCCCCEEECCCCCCEEEEEECCCCCHHHHHCCCCCEEEHHHHHHH
MLAAQKEGYPANAIRTRPGSEGQAVLVCRESNQLAHSGCQYAKTAYFETSAGYQAPANMC
HHHHHCCCCCCCCEECCCCCCCCEEEEECCCCHHHHHCHHHHHHHEEECCCCCCCHHHHH
EQHIPMAEPDSEESIPYAEPLDGSDDNIPLAEPVEETDGIPYATPI
HHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCC
>Mature Secondary Structure 
GLNDKSRKRNAASWRERIQDGEMSSRSQTERKKRPLPRPSYPGLPQEAPDPAVIPGPTR
CCCCCHHHCHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
RTGNSRNGPSRYDTPPQRRTSRPSPKEEYEGNPEREEENRPARAGTMRRPRFTIFGALLF
CCCCCCCCCCCCCCCCHHCCCCCCCHHHCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHH
VLLMPFRFIGSLTRNIRWFICWPLRILLGCCFVGIVIGAILVFLYGTISNRYDISEVKSN
HHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHC
IPERTVILDRKNRTIGTLHGENRKRVSLQEVPPVFIDALLLREDNRFYDHGGVDWIGVGR
CCCEEEEEECCCCEEEEEECCCCCEEEHHHCCHHHHHHHHHHCCCCEECCCCCCEEHHHH
AFAQVLKHKRATQGASTLTMQLAKITYNHRERNLHSKLTEVALAKRIEATYTKDEILETY
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
INRIFWGHTFLGIAAAARGYYDKEPRDLSLAECATLAGIIYGPNDFSPIKHPEEAKKVRD
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHEEECCCCCCCCCCCHHHHHHHH
IVLGLLKNEGKITEVQYQAALAEPIVTRTPQSRSEENYAMGLVRRELDAILEEEDIRLGG
HHHHHHHCCCCEEEHHHHHHHHCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEECC
LVVHTTLDLDLQNATLDAINKHMDALEARKDFKKHLASLQAKREKRGILKNKLTTKAEYE
EEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
ASLAAWKALPAEQKEKTQPPMPNYIQSAAVVMDNATGALLAVVGGRDAEESKLNRAIQSR
HHHHHHHCCCCCHHHCCCCCCCHHHHHHHHEEECCCCEEEEEECCCCCHHHHHHHHHHHH
RQTGSLFKPFIYATFFQQGNSADTRISDDRIAYGEIRGAANWSPRNSDGTYRGMKPASFG
HHCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEHHCCCCCCCCCCCCCCCCCCCCCCCCE
LILSRNTMSVRIGNRAGLSNVIQSAQLAGFHGNISRTPALYLGTWEASPLDIASAYSVFA
EEEECCEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHH
NGGVRPTPYIIDHITDSQGQPRFAITKSKRTVYSQRAANITSSILQQVCKPGGTAGKITT
CCCCCCCCEEEEECCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
LGFKSPCGGKTGTTNNYTNAWFAGYTSNLTCSVWVGFDSSTKILEKGYGGTLALPVWVDI
ECCCCCCCCCCCCCCCCCCCEEECCCCCCEEEEEECCCCCHHHHHCCCCCEEEHHHHHHH
MLAAQKEGYPANAIRTRPGSEGQAVLVCRESNQLAHSGCQYAKTAYFETSAGYQAPANMC
HHHHHCCCCCCCCEECCCCCCCCEEEEECCCCHHHHHCHHHHHHHEEECCCCCCCHHHHH
EQHIPMAEPDSEESIPYAEPLDGSDDNIPLAEPVEETDGIPYATPI
HHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10984043; 7901733 [H]