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The map label for this gene is proS

Identifier: 187734550

GI number: 187734550

Start: 41923

End: 43443

Strand: Direct

Name: proS

Synonym: Amuc_0034

Alternate gene names: 187734550

Gene position: 41923-43443 (Clockwise)

Preceding gene: 187734549

Following gene: 187734553

Centisome position: 1.57

GC content: 60.88

Gene sequence:

>1521_bases
ATGTCTCAGCAAACCGCAATCACCCCTACCCGCGCCCAGGATTTCCCCGAGTGGTACCAGCAAGTCATCAAGGCCGCCGA
CATGGCGGAAAATTCCGAGGTCCGCGGCTGCATGGTCATCAAGCCATGGGGCTACGCCATTTGGGAACTCATTCAAAAGG
ACCTGGACCAGCGCTTCAAGGACACCGGCCATACGAACGCCTATTTCCCCCTCCTGATTCCTATCTCCTATCTGGAAAAG
GAAGCGGAGCATGCTGAAGGCTTCGCCACGGAATGCGCCGTAGTCACCCACCACAGGCTGGAAGCGCAAAAGGATGAAGC
CACCGGCAAGACACGCATGATTCCCACCGGGGAGCTTACGGAACCCTTCGTCATCCGGCCCACCTCGGAAACCGTCATCG
GCGCGGCTTTCGCCCGCTGGACCTCCAGTTACCGGGACCTGCCCCTCAAAGTCAACCAGTGGTGCAACGTGATGCGCTGG
GAAATGAGGCCCCGAATCTTCCTGCGCACGGCGGAATTCCTGTGGCAGGAAGGGCATACGGCCCATGAAACCCGCGAGGA
AGCCATTGAGGAAACCCTCACCATGCACAAGGTTTATGAAGAATTCCAGCGGGACGTGCTCGCCATCCCCACCATTCCAG
GGGAAAAGACGGAGGCGGAACGCTTCCCCGGAGCGGAACAAACCTACACGGTGGAAGCCATGGTGCAGGACCGCAAGGCC
ATCCAGGCAGGGACCTCCCACTTCCTGGGGCAGAATTTCTCCAAGTCCCAGAACATCTGCTTCGCCGGAAGGGACAACAC
CCAGCAATTCGCATGGACAAGCTCCTGGGGCGTTTCCACCCGCATGATCGGAGCGCTCATCATGATGCACTCCGACGACG
ACGGACTCGTCTGCCCGCCCCGCGTCGCTCCCCAGCAAATCGTCATCATTCCCGTCACGCCCAAGGAAGAAAGCCGCCAG
GCCGTTCTGGACCACTGCGAGGAACTGGCGCGCACCCTCCGCGCCAAAACCTTCCACGGCCAGCCGCTGCGCGTGCTGGT
GGACAGGCGCGACCTGGGCGGCGGCGCCAAGAAATGGGAATGGGTGAAAAAAGGCGTGCCCGTGCGTCTGGAAATAGGCC
CCCGGGACCTGGAAAAAGGCTCCGTCTGCCTTCAGCGGCGCGACCGGCCCGCCAATGAAAAATCCTTCGTCCCGGAAACG
GAACTGATCGATACCGCTGCGGATATTCTCCAAAGCATCCAGGACACCCTGCTTCAGCGGGCCATTGCCTTCCGGGACTC
CCATATCCGCCCCGCCTCCACTCTGCGGGAATTGGAAGAAAACTTCTCCGGAGAGGGAGATGCAGATTGGCTCCAGGTGC
CGTGGGACGGGTCTCCGGAAGAAGAAGAAGAACTGGCTAAACGGCTGCGCATTTCCATCCGCTGCATCCCGCTCGGCGAG
CTGGGCCGCGGCGAACCGGCACCCTGCATCCTCACCGGACGTATGACGAAACGCCGTGTTCTCTGGGCCAGAAGCTACTG
A

Upstream 100 bases:

>100_bases
CGCCTACAGCAATTACCGGGAATACAAAAACGCCCCCTTTTAATATTATTCCGAGTTTCCTTTGCGGGCCCCGCCCGTTC
TATTCAACAAACCATCCAAT

Downstream 100 bases:

>100_bases
ACCCTGACACCCCCTTTCAACGGGATTGTTCCAAGACAGGCAAAACGGGCCTTTTCGGAACAATCCCGTTTTTTACGCCT
CTCTGCTTTCCCCAAAAACG

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 506; Mature: 505

Protein sequence:

>506_residues
MSQQTAITPTRAQDFPEWYQQVIKAADMAENSEVRGCMVIKPWGYAIWELIQKDLDQRFKDTGHTNAYFPLLIPISYLEK
EAEHAEGFATECAVVTHHRLEAQKDEATGKTRMIPTGELTEPFVIRPTSETVIGAAFARWTSSYRDLPLKVNQWCNVMRW
EMRPRIFLRTAEFLWQEGHTAHETREEAIEETLTMHKVYEEFQRDVLAIPTIPGEKTEAERFPGAEQTYTVEAMVQDRKA
IQAGTSHFLGQNFSKSQNICFAGRDNTQQFAWTSSWGVSTRMIGALIMMHSDDDGLVCPPRVAPQQIVIIPVTPKEESRQ
AVLDHCEELARTLRAKTFHGQPLRVLVDRRDLGGGAKKWEWVKKGVPVRLEIGPRDLEKGSVCLQRRDRPANEKSFVPET
ELIDTAADILQSIQDTLLQRAIAFRDSHIRPASTLRELEENFSGEGDADWLQVPWDGSPEEEEELAKRLRISIRCIPLGE
LGRGEPAPCILTGRMTKRRVLWARSY

Sequences:

>Translated_506_residues
MSQQTAITPTRAQDFPEWYQQVIKAADMAENSEVRGCMVIKPWGYAIWELIQKDLDQRFKDTGHTNAYFPLLIPISYLEK
EAEHAEGFATECAVVTHHRLEAQKDEATGKTRMIPTGELTEPFVIRPTSETVIGAAFARWTSSYRDLPLKVNQWCNVMRW
EMRPRIFLRTAEFLWQEGHTAHETREEAIEETLTMHKVYEEFQRDVLAIPTIPGEKTEAERFPGAEQTYTVEAMVQDRKA
IQAGTSHFLGQNFSKSQNICFAGRDNTQQFAWTSSWGVSTRMIGALIMMHSDDDGLVCPPRVAPQQIVIIPVTPKEESRQ
AVLDHCEELARTLRAKTFHGQPLRVLVDRRDLGGGAKKWEWVKKGVPVRLEIGPRDLEKGSVCLQRRDRPANEKSFVPET
ELIDTAADILQSIQDTLLQRAIAFRDSHIRPASTLRELEENFSGEGDADWLQVPWDGSPEEEEELAKRLRISIRCIPLGE
LGRGEPAPCILTGRMTKRRVLWARSY
>Mature_505_residues
SQQTAITPTRAQDFPEWYQQVIKAADMAENSEVRGCMVIKPWGYAIWELIQKDLDQRFKDTGHTNAYFPLLIPISYLEKE
AEHAEGFATECAVVTHHRLEAQKDEATGKTRMIPTGELTEPFVIRPTSETVIGAAFARWTSSYRDLPLKVNQWCNVMRWE
MRPRIFLRTAEFLWQEGHTAHETREEAIEETLTMHKVYEEFQRDVLAIPTIPGEKTEAERFPGAEQTYTVEAMVQDRKAI
QAGTSHFLGQNFSKSQNICFAGRDNTQQFAWTSSWGVSTRMIGALIMMHSDDDGLVCPPRVAPQQIVIIPVTPKEESRQA
VLDHCEELARTLRAKTFHGQPLRVLVDRRDLGGGAKKWEWVKKGVPVRLEIGPRDLEKGSVCLQRRDRPANEKSFVPETE
LIDTAADILQSIQDTLLQRAIAFRDSHIRPASTLRELEENFSGEGDADWLQVPWDGSPEEEEELAKRLRISIRCIPLGEL
GRGEPAPCILTGRMTKRRVLWARSY

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro)

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily

Homologues:

Organism=Homo sapiens, GI62241042, Length=517, Percent_Identity=41.0058027079304, Blast_Score=354, Evalue=1e-97,
Organism=Caenorhabditis elegans, GI71991342, Length=517, Percent_Identity=36.9439071566731, Blast_Score=341, Evalue=6e-94,
Organism=Caenorhabditis elegans, GI71991346, Length=378, Percent_Identity=42.0634920634921, Blast_Score=313, Evalue=2e-85,
Organism=Saccharomyces cerevisiae, GI6321808, Length=529, Percent_Identity=38.5633270321361, Blast_Score=343, Evalue=4e-95,
Organism=Drosophila melanogaster, GI24649468, Length=523, Percent_Identity=37.093690248566, Blast_Score=310, Evalue=1e-84,
Organism=Drosophila melanogaster, GI24649466, Length=523, Percent_Identity=37.093690248566, Blast_Score=310, Evalue=2e-84,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_AKKM8 (B2UL92)

Other databases:

- EMBL:   CP001071
- RefSeq:   YP_001876662.1
- ProteinModelPortal:   B2UL92
- SMR:   B2UL92
- GeneID:   6275179
- GenomeReviews:   CP001071_GR
- KEGG:   amu:Amuc_0034
- HOGENOM:   HBG334108
- OMA:   PGWKFNE
- GO:   GO:0005737
- HAMAP:   MF_01571
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR004499
- InterPro:   IPR017449
- InterPro:   IPR016061
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.30.110.30
- PANTHER:   PTHR11451:SF6
- TIGRFAMs:   TIGR00408

Pfam domain/function: PF03129 HGTP_anticodon; PF09180 ProRS-C_1; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd; SSF64586 Pro-tRNA_synth_II_C

EC number: =6.1.1.15

Molecular weight: Translated: 57738; Mature: 57607

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQQTAITPTRAQDFPEWYQQVIKAADMAENSEVRGCMVIKPWGYAIWELIQKDLDQRFK
CCCCCCCCCCHHHCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHH
DTGHTNAYFPLLIPISYLEKEAEHAEGFATECAVVTHHRLEAQKDEATGKTRMIPTGELT
HCCCCCCEEHHEEEHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHCCCCEEECCCCCCC
EPFVIRPTSETVIGAAFARWTSSYRDLPLKVNQWCNVMRWEMRPRIFLRTAEFLWQEGHT
CCEEEECCCCHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHCCCHHHHHHHHHHHHCCCC
AHETREEAIEETLTMHKVYEEFQRDVLAIPTIPGEKTEAERFPGAEQTYTVEAMVQDRKA
HHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCHHHHCCCCCCEEEHHHHHHHHHH
IQAGTSHFLGQNFSKSQNICFAGRDNTQQFAWTSSWGVSTRMIGALIMMHSDDDGLVCPP
HHHHHHHHHCCCCCCCCCEEEECCCCCCEEEEECCCCCHHHHHHEEEEEECCCCCCCCCC
RVAPQQIVIIPVTPKEESRQAVLDHCEELARTLRAKTFHGQPLRVLVDRRDLGGGAKKWE
CCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHH
WVKKGVPVRLEIGPRDLEKGSVCLQRRDRPANEKSFVPETELIDTAADILQSIQDTLLQR
HHHCCCCEEEEECCCCCCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AIAFRDSHIRPASTLRELEENFSGEGDADWLQVPWDGSPEEEEELAKRLRISIRCIPLGE
HHHHHCCCCCHHHHHHHHHHHCCCCCCCCEEECCCCCCCCHHHHHHHHHEEEEEEEECCC
LGRGEPAPCILTGRMTKRRVLWARSY
CCCCCCCCEEEECCCCHHEEEEECCC
>Mature Secondary Structure 
SQQTAITPTRAQDFPEWYQQVIKAADMAENSEVRGCMVIKPWGYAIWELIQKDLDQRFK
CCCCCCCCCHHHCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHH
DTGHTNAYFPLLIPISYLEKEAEHAEGFATECAVVTHHRLEAQKDEATGKTRMIPTGELT
HCCCCCCEEHHEEEHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHCCCCEEECCCCCCC
EPFVIRPTSETVIGAAFARWTSSYRDLPLKVNQWCNVMRWEMRPRIFLRTAEFLWQEGHT
CCEEEECCCCHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHCCCHHHHHHHHHHHHCCCC
AHETREEAIEETLTMHKVYEEFQRDVLAIPTIPGEKTEAERFPGAEQTYTVEAMVQDRKA
HHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCHHHHCCCCCCEEEHHHHHHHHHH
IQAGTSHFLGQNFSKSQNICFAGRDNTQQFAWTSSWGVSTRMIGALIMMHSDDDGLVCPP
HHHHHHHHHCCCCCCCCCEEEECCCCCCEEEEECCCCCHHHHHHEEEEEECCCCCCCCCC
RVAPQQIVIIPVTPKEESRQAVLDHCEELARTLRAKTFHGQPLRVLVDRRDLGGGAKKWE
CCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHH
WVKKGVPVRLEIGPRDLEKGSVCLQRRDRPANEKSFVPETELIDTAADILQSIQDTLLQR
HHHCCCCEEEEECCCCCCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AIAFRDSHIRPASTLRELEENFSGEGDADWLQVPWDGSPEEEEELAKRLRISIRCIPLGE
HHHHHCCCCCHHHHHHHHHHHCCCCCCCCEEECCCCCCCCHHHHHHHHHEEEEEEEECCC
LGRGEPAPCILTGRMTKRRVLWARSY
CCCCCCCCEEEECCCCHHEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA