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The map label for this gene is rpoN [H]

Identifier: 187734537

GI number: 187734537

Start: 26737

End: 28134

Strand: Reverse

Name: rpoN [H]

Synonym: Amuc_0021

Alternate gene names: 187734537

Gene position: 28134-26737 (Counterclockwise)

Preceding gene: 187734543

Following gene: 187734536

Centisome position: 1.06

GC content: 61.66

Gene sequence:

>1398_bases
ATGAGTGAGGTATCCCTGCAGCATGGAATGACGCGCCAGATGGCCGTCTCCCAGTCCATGCAGACAGGGATGCAGATTCT
TCAGGCTTCTGCTTTGGAATTAAGGCAGATTATCCGTCAGGCGCTGGAAACCAATCCCATGCTTGAGGAGTTGCCGGACG
CTTCCCCGGAAGCGCTGGAAGACGGTCCGGACGGGGATGCCTGGAATGAGCGGGAGGACGGGTGGAACGAGTTTACGGCG
GAAGGCCGCCTGTCTGGGGACGCGGCCGCCCGCCGTGATTTCATGTATGAATCCGTCGTGGCTCCGGAATCCCTGAAAAC
CCATTTGATGGATCAGGCGCAGCATTCCGCATTGACGGGCCGCGCCCGGGACGCCCTGTTCCTGCTGATTGACGCTTTGG
ACGAACGCGGGTTTCTGACGGAGTCTCCGCAGGAGCTGGAAGAGCAGGGGTGTTTCAGCATGCGGGACATGGAGGAAGCT
CTGGCTGCGCTGAGGGAAATGGACCCGCCTGGAGTGGGGGCGGCGAATTTAAGGGATTCCCTGCTGATCCAGCTTGAACA
GCGCGGTTTGAAGCGTTCGCTTGCTTTCCGCCTGGTCAAGCGCTGCTGGCGGGAACTGGCGGCGCACAAGTATGAGGAAG
CCGCACGCCTGCTGGACGTGGAACCGGGGGCCGTGGCGGCGGCTCTGGAGGTGATTAGGAGCCTGACTCCTGATCCAGGG
GGGGCCTACGCCCCCGGCGGCAACCCTCATCTGCTGCCTGACGTCATTGTGGAGGAGGGGCCTTCCGGCGTGCTGGAGGT
TATCCTCACTTCCGAGTATCTTCCGCGCCTGTCCATGAATGAACGGTATATGGAGTTAATGGCTGAGGGTTCCGGAAGCC
GGGAGCTGAGGCAATATCTCCGCAGGGCGTTCCGTGAGGGACAGGAGCTGTTGCGGGCTTTGGATATGAGGCAGGAGACC
GTTTTGCGCCTGGCCCGTGTCATTGTGCGGAGGCAGGAGGATTTTTTCAGGTCCGGCCCGTCCCGCCTGAAGGCCATGGG
CATGGAAGAAGTGGCGGAGGAGATGGGGGTTCATGTTTCCACGGTTTCCCGGGCGTGCCGGGACAAGTACCTGTTGTGCA
GGTGGGGGATGAAAGAATTGAGATCCTTTTTCAGTGCGGGAGTTCCGTCTGAAGGCGGCGTTTCCCCGGACGGGGCGGCT
TCCGGAGCCGTGGCTGCCGGCGCCGTCCAAGAGCTGATGCGGCGGCTGATTGCGGAGGAAGATTCTTCCAAACCCCTCAG
TGACGCCGGGCTGGCGGCCGCGCTGCGGGAAAAAGGGGTGAACATCGCCCGCAGGACGGTGGCCAAATATCGGGAGCAGA
TGAAGATACTGCCCGCTTCCCTGCGCCGGGGAATATGA

Upstream 100 bases:

>100_bases
AAAGTGGTGGCCTTTTTTGCTGCTGCGGTGTGCGGGCAGCTTTTGCTGCAGGGTGTTCCCCCTTGCCAGATTCCGGGGGG
TGGTGTAGCTTGTGGGCGGT

Downstream 100 bases:

>100_bases
CAGTCCCCTATATTTTGAATTGTTTATTAATAATTTAACAATCAATGTGTTGTGTCTTTTGTTGATAAGGCGCTTTTTTT
GTTTTTCTTTCCGACGGAAG

Product: RNA polymerase, sigma 54 subunit, RpoN

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 465; Mature: 464

Protein sequence:

>465_residues
MSEVSLQHGMTRQMAVSQSMQTGMQILQASALELRQIIRQALETNPMLEELPDASPEALEDGPDGDAWNEREDGWNEFTA
EGRLSGDAAARRDFMYESVVAPESLKTHLMDQAQHSALTGRARDALFLLIDALDERGFLTESPQELEEQGCFSMRDMEEA
LAALREMDPPGVGAANLRDSLLIQLEQRGLKRSLAFRLVKRCWRELAAHKYEEAARLLDVEPGAVAAALEVIRSLTPDPG
GAYAPGGNPHLLPDVIVEEGPSGVLEVILTSEYLPRLSMNERYMELMAEGSGSRELRQYLRRAFREGQELLRALDMRQET
VLRLARVIVRRQEDFFRSGPSRLKAMGMEEVAEEMGVHVSTVSRACRDKYLLCRWGMKELRSFFSAGVPSEGGVSPDGAA
SGAVAAGAVQELMRRLIAEEDSSKPLSDAGLAAALREKGVNIARRTVAKYREQMKILPASLRRGI

Sequences:

>Translated_465_residues
MSEVSLQHGMTRQMAVSQSMQTGMQILQASALELRQIIRQALETNPMLEELPDASPEALEDGPDGDAWNEREDGWNEFTA
EGRLSGDAAARRDFMYESVVAPESLKTHLMDQAQHSALTGRARDALFLLIDALDERGFLTESPQELEEQGCFSMRDMEEA
LAALREMDPPGVGAANLRDSLLIQLEQRGLKRSLAFRLVKRCWRELAAHKYEEAARLLDVEPGAVAAALEVIRSLTPDPG
GAYAPGGNPHLLPDVIVEEGPSGVLEVILTSEYLPRLSMNERYMELMAEGSGSRELRQYLRRAFREGQELLRALDMRQET
VLRLARVIVRRQEDFFRSGPSRLKAMGMEEVAEEMGVHVSTVSRACRDKYLLCRWGMKELRSFFSAGVPSEGGVSPDGAA
SGAVAAGAVQELMRRLIAEEDSSKPLSDAGLAAALREKGVNIARRTVAKYREQMKILPASLRRGI
>Mature_464_residues
SEVSLQHGMTRQMAVSQSMQTGMQILQASALELRQIIRQALETNPMLEELPDASPEALEDGPDGDAWNEREDGWNEFTAE
GRLSGDAAARRDFMYESVVAPESLKTHLMDQAQHSALTGRARDALFLLIDALDERGFLTESPQELEEQGCFSMRDMEEAL
AALREMDPPGVGAANLRDSLLIQLEQRGLKRSLAFRLVKRCWRELAAHKYEEAARLLDVEPGAVAAALEVIRSLTPDPGG
AYAPGGNPHLLPDVIVEEGPSGVLEVILTSEYLPRLSMNERYMELMAEGSGSRELRQYLRRAFREGQELLRALDMRQETV
LRLARVIVRRQEDFFRSGPSRLKAMGMEEVAEEMGVHVSTVSRACRDKYLLCRWGMKELRSFFSAGVPSEGGVSPDGAAS
GAVAAGAVQELMRRLIAEEDSSKPLSDAGLAAALREKGVNIARRTVAKYREQMKILPASLRRGI

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of the nitrogen fixation genes. The open complex (sigma-54 and core

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=483, Percent_Identity=31.4699792960663, Blast_Score=231, Evalue=6e-62,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 51481; Mature: 51350

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEVSLQHGMTRQMAVSQSMQTGMQILQASALELRQIIRQALETNPMLEELPDASPEALE
CCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCHHHC
DGPDGDAWNEREDGWNEFTAEGRLSGDAAARRDFMYESVVAPESLKTHLMDQAQHSALTG
CCCCCCCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
RARDALFLLIDALDERGFLTESPQELEEQGCFSMRDMEEALAALREMDPPGVGAANLRDS
HHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
LLIQLEQRGLKRSLAFRLVKRCWRELAAHKYEEAARLLDVEPGAVAAALEVIRSLTPDPG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCC
GAYAPGGNPHLLPDVIVEEGPSGVLEVILTSEYLPRLSMNERYMELMAEGSGSRELRQYL
CCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHH
RRAFREGQELLRALDMRQETVLRLARVIVRRQEDFFRSGPSRLKAMGMEEVAEEMGVHVS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHCCHHH
TVSRACRDKYLLCRWGMKELRSFFSAGVPSEGGVSPDGAASGAVAAGAVQELMRRLIAEE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
DSSKPLSDAGLAAALREKGVNIARRTVAKYREQMKILPASLRRGI
CCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SEVSLQHGMTRQMAVSQSMQTGMQILQASALELRQIIRQALETNPMLEELPDASPEALE
CCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCHHHC
DGPDGDAWNEREDGWNEFTAEGRLSGDAAARRDFMYESVVAPESLKTHLMDQAQHSALTG
CCCCCCCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
RARDALFLLIDALDERGFLTESPQELEEQGCFSMRDMEEALAALREMDPPGVGAANLRDS
HHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
LLIQLEQRGLKRSLAFRLVKRCWRELAAHKYEEAARLLDVEPGAVAAALEVIRSLTPDPG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCC
GAYAPGGNPHLLPDVIVEEGPSGVLEVILTSEYLPRLSMNERYMELMAEGSGSRELRQYL
CCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHH
RRAFREGQELLRALDMRQETVLRLARVIVRRQEDFFRSGPSRLKAMGMEEVAEEMGVHVS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHCCHHH
TVSRACRDKYLLCRWGMKELRSFFSAGVPSEGGVSPDGAASGAVAAGAVQELMRRLIAEE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
DSSKPLSDAGLAAALREKGVNIARRTVAKYREQMKILPASLRRGI
CCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2999700; 2695747 [H]