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The map label for this gene is ynfE

Identifier: 187733706

GI number: 187733706

Start: 1614436

End: 1616862

Strand: Direct

Name: ynfE

Synonym: SbBS512_E1773

Alternate gene names: 187733706

Gene position: 1614436-1616862 (Clockwise)

Preceding gene: 187734083

Following gene: 187734181

Centisome position: 34.97

GC content: 50.39

Gene sequence:

>2427_bases
ATGTCCAAAAATGACCGAATGGTGGGCATCAGCCGCAGAACACTCGTTAAATCAACCGCGATAGGTTCTCTGGCGCTGGC
TGCAGGCGGTTTTTCTTTACCGTTCACCCTGCGCAGTGCAGCGGCAACGGTACAACAGGCCAGCGAAAAAGTGATCTGGG
GTGCCTGTTCCGTCAACTGTGGTAGCCGCTGTGCACTTCGTCTACATGTTAAAGATAATGAAGTGACCTGGGTGGAAACT
GACAATACCGGCAGCGATGAGTACGGCAACCATCAGGTACGCGCCTGTTTGCGCGGTCGCTCCATCCGCCGGCGTATTAA
TCATCCCGATCGCTTGAATTACCCAATGAAACGCGTGGGTAAACGCGGTGAAGGCAAATTCGAACGGATTAGCTGGGATG
AAGCCCTGGATACTATCGCCAGTAGCCTGAAAAAAACCGTCGAACAATATGGCAATGAGGCTGTATATATTCAGTACTCT
TCGGGGATTGTTGGCGGCAATATGACCCGCTCTTCGCCATCAGCCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCTG
TTCACTCAATCAGTATGGCTCCTACAGCACCGAGCAAATTTCCTGTGCCATGCCCTACACCTACGGCAGTAATGATGGCA
ACAGCACCACGGATATTGAAAACAGCAAGCTGGTCGTGATGTTTGGTAACAACCCGGCAGAAACCCGCATGAGCGGCGGT
GGCATCACTTATCTTCTTGAAAAAGCGCGCGAGAAATCGAACGCCAAAATGATTGTTATCGATCCGCGATATACCGATAC
GGCTGCTGGTCGTGAAGATGAATGGCTTCCTATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTAT
TGATTAACGAAAATCTCGTTGATCAACCTTTTCTCGATAAATACTGCGTCGGCTATGACGAAAAAACCTTACCCGCAGAT
GCACCAAAAAATGGTCACTATAAAGCCTATATTCTTGGTGAAGGTGATGATAATACAGCGAAAACGCCGCAGTGGGCTTC
GCAAATTACCGGTATCCCGGTGGACCGTATCATCAAACTGGCGCGTGAAATTGGCACAGCAAAACCCGCTTATATCTGCC
AGGGCTGGGGGCCGCAACGCCAGGCAAACGGCGAACTGACTGCACGCGCTATTGCTATGTTGCCTATTTTGACGGGCAAT
GTCGGCATCAGCGGCGGAAATAGTGGCGCGAGTGAATCGACCTATACCATTACCATAGAACGCCTGCCGGTGCTGGATAA
TCCGGTCAAAACGTCAATCTCCTGCTTCAGCTGGACAGATGCTATCGATCATGGTCCGCAAATGACGGCAATCCGCGACG
GCGTCCGCGGCAAAGATAAACTGGATGTGCCCATTAAGTTCATCTGGAACTACGCGGGAAATACCCTCGTTAATCAGCAT
TCTGACATCAACAAAACGCATGAAATTCTGCAGGACGAATCGAAATGCGAAATGATTGTGGTCATCGAAAACTTTATGAC
CTCATCGGCAAAATATGCCGACATTCTGCTGCCAGACCTGATGACCGTTGAGCAGGAAGATATTATTCCTAACGACTACG
CCGGTAACATGGGATATCTCATTTTCCTCCAGCCTGTCACCAGCGAAAAATTCGAACGCAAACCGATTTACTGGATCCTG
AGTGAAGTCGCGAAACGTCTTGGACCTGACGTCTATCAAAAGTTCACAGAAGGTCGCACGCAGGAACAATGGTTACAACA
TCTGTACGCCAAAATGCTTGCCAAAGATCCGGCGTTACCGTCTTACGACGAACTGAAAAAAATGGGTATCTATAAGCGTA
AAGATCCCAATGGCCATTTTGTCGCCTACAAAGCATTTCGTGACGACCCCGAGGCAAATCCACTTAAAACGCCTTCCGGT
AAGATTGAAATTTATTCCAGCAGGCTGGCGGAAATTGCCCGTACCTGGGAACTGGAAAAAGATGAAGTGATAAGCCCATT
GCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATT
ACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCT
ATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGC
CAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATA
AAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACT
AATCTGGTCGAGATCGAAAAAATATAA

Upstream 100 bases:

>100_bases
ATAATTCTTAACCCAAAATGGGTAGACCCCCTCTATTGTTAGCGCGCTAAATATTCAATACATAAACATTTATATAACGA
TAAAGAACAGGGAGTGAGTT

Downstream 100 bases:

>100_bases
CCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACCACAGA
GGCGCTCATGAAGGCTGAAA

Product: anaerobic dimethyl sulfoxide reductase, A subunit YnfE

Products: NA

Alternate protein names: DMSO reductase [H]

Number of amino acids: Translated: 808; Mature: 807

Protein sequence:

>808_residues
MSKNDRMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAATVQQASEKVIWGACSVNCGSRCALRLHVKDNEVTWVET
DNTGSDEYGNHQVRACLRGRSIRRRINHPDRLNYPMKRVGKRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
SGIVGGNMTRSSPSASAVKRLMNCYGCSLNQYGSYSTEQISCAMPYTYGSNDGNSTTDIENSKLVVMFGNNPAETRMSGG
GITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWLPIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPAD
APKNGHYKAYILGEGDDNTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIAMLPILTGN
VGISGGNSGASESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTAIRDGVRGKDKLDVPIKFIWNYAGNTLVNQH
SDINKTHEILQDESKCEMIVVIENFMTSSAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWIL
SEVAKRLGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYKAFRDDPEANPLKTPSG
KIEIYSSRLAEIARTWELEKDEVISPLPVYASTFEGWNSPERRTFPLQLFGFHYKSRTHSTYGNIDLLKAACRQEVWINP
IDAQKRGIANGDMVRVFNHRGEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQHT
NLVEIEKI

Sequences:

>Translated_808_residues
MSKNDRMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAATVQQASEKVIWGACSVNCGSRCALRLHVKDNEVTWVET
DNTGSDEYGNHQVRACLRGRSIRRRINHPDRLNYPMKRVGKRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
SGIVGGNMTRSSPSASAVKRLMNCYGCSLNQYGSYSTEQISCAMPYTYGSNDGNSTTDIENSKLVVMFGNNPAETRMSGG
GITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWLPIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPAD
APKNGHYKAYILGEGDDNTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIAMLPILTGN
VGISGGNSGASESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTAIRDGVRGKDKLDVPIKFIWNYAGNTLVNQH
SDINKTHEILQDESKCEMIVVIENFMTSSAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWIL
SEVAKRLGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYKAFRDDPEANPLKTPSG
KIEIYSSRLAEIARTWELEKDEVISPLPVYASTFEGWNSPERRTFPLQLFGFHYKSRTHSTYGNIDLLKAACRQEVWINP
IDAQKRGIANGDMVRVFNHRGEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQHT
NLVEIEKI
>Mature_807_residues
SKNDRMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAATVQQASEKVIWGACSVNCGSRCALRLHVKDNEVTWVETD
NTGSDEYGNHQVRACLRGRSIRRRINHPDRLNYPMKRVGKRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYSS
GIVGGNMTRSSPSASAVKRLMNCYGCSLNQYGSYSTEQISCAMPYTYGSNDGNSTTDIENSKLVVMFGNNPAETRMSGGG
ITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWLPIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPADA
PKNGHYKAYILGEGDDNTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIAMLPILTGNV
GISGGNSGASESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTAIRDGVRGKDKLDVPIKFIWNYAGNTLVNQHS
DINKTHEILQDESKCEMIVVIENFMTSSAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILS
EVAKRLGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYKAFRDDPEANPLKTPSGK
IEIYSSRLAEIARTWELEKDEVISPLPVYASTFEGWNSPERRTFPLQLFGFHYKSRTHSTYGNIDLLKAACRQEVWINPI
DAQKRGIANGDMVRVFNHRGEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQHTN
LVEIEKI

Specific function: Terminal reductase during anaerobic growth on various sulfoxide and N-oxide compounds [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI1787870, Length=808, Percent_Identity=98.7623762376238, Blast_Score=1666, Evalue=0.0,
Organism=Escherichia coli, GI171474008, Length=801, Percent_Identity=70.2871410736579, Blast_Score=1222, Evalue=0.0,
Organism=Escherichia coli, GI87081797, Length=805, Percent_Identity=66.9565217391304, Blast_Score=1130, Evalue=0.0,
Organism=Escherichia coli, GI145693196, Length=753, Percent_Identity=30.4116865869854, Blast_Score=286, Evalue=3e-78,
Organism=Escherichia coli, GI87081994, Length=848, Percent_Identity=29.0094339622642, Blast_Score=266, Evalue=5e-72,
Organism=Escherichia coli, GI1787231, Length=760, Percent_Identity=29.6052631578947, Blast_Score=264, Evalue=1e-71,
Organism=Escherichia coli, GI3868721, Length=778, Percent_Identity=25.0642673521851, Blast_Score=136, Evalue=5e-33,
Organism=Escherichia coli, GI3868720, Length=458, Percent_Identity=26.4192139737991, Blast_Score=99, Evalue=1e-21,
Organism=Escherichia coli, GI3868719, Length=456, Percent_Identity=24.3421052631579, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1787741, Length=428, Percent_Identity=24.7663551401869, Blast_Score=83, Evalue=7e-17,
Organism=Escherichia coli, GI1788534, Length=889, Percent_Identity=22.0472440944882, Blast_Score=80, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011888
- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655
- InterPro:   IPR006311 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: 1.8.99.- [C]

Molecular weight: Translated: 89706; Mature: 89575

Theoretical pI: Translated: 8.10; Mature: 8.10

Prosite motif: PS00932 MOLYBDOPTERIN_PROK_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKNDRMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAATVQQASEKVIWGACSVNC
CCCCCCEEECHHHHHHHHHCCCHHEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCC
GSRCALRLHVKDNEVTWVETDNTGSDEYGNHQVRACLRGRSIRRRINHPDRLNYPMKRVG
CCEEEEEEEEECCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHC
KRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYSSGIVGGNMTRSSPSASAVKR
CCCCCCEEECCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEECCCCCCCCCCHHHHHH
LMNCYGCSLNQYGSYSTEQISCAMPYTYGSNDGNSTTDIENSKLVVMFGNNPAETRMSGG
HHHHHCCCCCCCCCCCCCEEEEEECEEECCCCCCCCCCCCCCEEEEEECCCCHHCEECCC
GITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWLPIRPGTDAALVAGIAWVLINENLV
CHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCEEECCCCCCHHHHHHEEEEEECCCCC
DQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGEGDDNTAKTPQWASQITGIPVDRIIKL
CCCCHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHCCCCHHHHHHH
AREIGTAKPAYICQGWGPQRQANGELTARAIAMLPILTGNVGISGGNSGASESTYTITIE
HHHHCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHEEECCEEECCCCCCCCCCEEEEEEE
RLPVLDNPVKTSISCFSWTDAIDHGPQMTAIRDGVRGKDKLDVPIKFIWNYAGNTLVNQH
ECCCCCCCHHCCEEEEEEHHHHCCCCCCHHHHCCCCCCCCCCCCEEEEEECCCCCEECCC
SDINKTHEILQDESKCEMIVVIENFMTSSAKYADILLPDLMTVEQEDIIPNDYAGNMGYL
CCCHHHHHHHCCCCCCCEEEEEEHHHCCCCCHHHHHHHHHHHCCHHCCCCCCCCCCCEEE
IFLQPVTSEKFERKPIYWILSEVAKRLGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALP
EEEECCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCC
SYDELKKMGIYKRKDPNGHFVAYKAFRDDPEANPLKTPSGKIEIYSSRLAEIARTWELEK
CHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEHHHHHHHHHHHCCCCH
DEVISPLPVYASTFEGWNSPERRTFPLQLFGFHYKSRTHSTYGNIDLLKAACRQEVWINP
HHCCCCCCHHHHHHCCCCCCCCEECCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCC
IDAQKRGIANGDMVRVFNHRGEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGC
CCHHHCCCCCCCEEEEECCCCCEEECCCCCCHHCCCCHHHCCCCCEECCCCCCCCCCCCH
VNTLTTLRPSPLAKGNPQHTNLVEIEKI
HHHHHHCCCCCCCCCCCCCCCEEEEECC
>Mature Secondary Structure 
SKNDRMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAATVQQASEKVIWGACSVNC
CCCCCEEECHHHHHHHHHCCCHHEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCC
GSRCALRLHVKDNEVTWVETDNTGSDEYGNHQVRACLRGRSIRRRINHPDRLNYPMKRVG
CCEEEEEEEEECCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHC
KRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYSSGIVGGNMTRSSPSASAVKR
CCCCCCEEECCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEECCCCCCCCCCHHHHHH
LMNCYGCSLNQYGSYSTEQISCAMPYTYGSNDGNSTTDIENSKLVVMFGNNPAETRMSGG
HHHHHCCCCCCCCCCCCCEEEEEECEEECCCCCCCCCCCCCCEEEEEECCCCHHCEECCC
GITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWLPIRPGTDAALVAGIAWVLINENLV
CHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCEEECCCCCCHHHHHHEEEEEECCCCC
DQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGEGDDNTAKTPQWASQITGIPVDRIIKL
CCCCHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHCCCCHHHHHHH
AREIGTAKPAYICQGWGPQRQANGELTARAIAMLPILTGNVGISGGNSGASESTYTITIE
HHHHCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHEEECCEEECCCCCCCCCCEEEEEEE
RLPVLDNPVKTSISCFSWTDAIDHGPQMTAIRDGVRGKDKLDVPIKFIWNYAGNTLVNQH
ECCCCCCCHHCCEEEEEEHHHHCCCCCCHHHHCCCCCCCCCCCCEEEEEECCCCCEECCC
SDINKTHEILQDESKCEMIVVIENFMTSSAKYADILLPDLMTVEQEDIIPNDYAGNMGYL
CCCHHHHHHHCCCCCCCEEEEEEHHHCCCCCHHHHHHHHHHHCCHHCCCCCCCCCCCEEE
IFLQPVTSEKFERKPIYWILSEVAKRLGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALP
EEEECCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCC
SYDELKKMGIYKRKDPNGHFVAYKAFRDDPEANPLKTPSGKIEIYSSRLAEIARTWELEK
CHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEHHHHHHHHHHHCCCCH
DEVISPLPVYASTFEGWNSPERRTFPLQLFGFHYKSRTHSTYGNIDLLKAACRQEVWINP
HHCCCCCCHHHHHHCCCCCCCCEECCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCC
IDAQKRGIANGDMVRVFNHRGEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGC
CCHHHCCCCCCCEEEEECCCCCEEECCCCCCHHCCCCHHHCCCCCEECCCCCCCCCCCCH
VNTLTTLRPSPLAKGNPQHTNLVEIEKI
HHHHHHCCCCCCCCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: May Bind 4Fe-4S Cluster. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]