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The map label for this gene is hpaI

Identifier: 187733380

GI number: 187733380

Start: 4558156

End: 4558944

Strand: Reverse

Name: hpaI

Synonym: SbBS512_E4885

Alternate gene names: 187733380

Gene position: 4558944-4558156 (Counterclockwise)

Preceding gene: 187730336

Following gene: 187730067

Centisome position: 98.76

GC content: 60.2

Gene sequence:

>789_bases
ATGGAAAACAGCTTTAAAGCGGCGCTGAAAGCAGGCCGTCCGCAGATCGGATTATGGCTGGGGCTGAGTAGCAGCTACAG
CGCAGAGTTACTGGCCGGAGCAGGATTCGACTGGTTGTTGATCGACGGTGAGCACGCGCCGAATAACGTGCAAACCGTGC
TCACCCAGCTACAGGCGATTGCGCCCTACCCCAACCAGCCGGTAGTACGTCCGTCGTGGAACGATCCGGTGCAAATCAAA
CAACTGCTGGACGTCGGCACACAAACCTTGCTGGTGCCGATGGTACAAAACGCTGACGAAGCCCGTGAAGCGGTACGCGC
CACCCGTTATCCCCCCGCCGGTATTCGCGGTGTGGGCAGTGCGCTGGCCCGCGCCTCGCGCTGGAATCGTATTCCTGATT
ACCTGCAAAAAGCCAACGATCAAATGTGCGTGCTGGTGCAGATCGAAACGCGTGAGGCAATGAAGAACTTACCGCAGATT
CTGGACGTGGAAGGCGTCGACGGCGTGTTTATCGGCCCGGCGGATCTGAGCGCCGATATGGGTTATGCCGGTAATCCGCA
GCACCCGGAAGTACAGGCCGCCATTGAGCAGGCGATCGTGCAGATCCGTGAATCGGGCAAAGCGCCGGGGATCCTGATCG
CCAATGAGCAACTGGCAAAACGCTATCTGGAACTGGGCGCGCTGTTTGTCGCCGTCGGCGTTGACACCACCCTGCTCGCC
CGCGCCGCTGAAGCGCTGGCAGCACGGTTTGGTGCGCAGGCCACCGCCGTGAAGCCCGGCGTGTATTAA

Upstream 100 bases:

>100_bases
TCGTTCACCCGCCCGGTTCCGGCGCGTAAGGGCGACACCTTCCACGTCGATTACGGCAACATGGGTTCCATTAGCTGCCG
CTTTGTTTAAGGAGAGAACG

Downstream 100 bases:

>100_bases
TGCCGGATGCGCTGCACTTATCCGGCCTACACTCGCACCAAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAA
AAAGCCGTACCAAATCGCGG

Product: 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Products: NA

Alternate protein names: 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase; HHED aldolase; 4-hydroxy-2-ketoheptane-1,7-dioate aldolase; HKHD aldolase [H]

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGEHAPNNVQTVLTQLQAIAPYPNQPVVRPSWNDPVQIK
QLLDVGTQTLLVPMVQNADEAREAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIRESGKAPGILIANEQLAKRYLELGALFVAVGVDTTLLA
RAAEALAARFGAQATAVKPGVY

Sequences:

>Translated_262_residues
MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGEHAPNNVQTVLTQLQAIAPYPNQPVVRPSWNDPVQIK
QLLDVGTQTLLVPMVQNADEAREAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIRESGKAPGILIANEQLAKRYLELGALFVAVGVDTTLLA
RAAEALAARFGAQATAVKPGVY
>Mature_262_residues
MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGEHAPNNVQTVLTQLQAIAPYPNQPVVRPSWNDPVQIK
QLLDVGTQTLLVPMVQNADEAREAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIRESGKAPGILIANEQLAKRYLELGALFVAVGVDTTLLA
RAAEALAARFGAQATAVKPGVY

Specific function: Catalyzes the reversible retro-aldol cleavage of 4- hydroxy-2-ketoheptane-1,7-dioate (HKHD) to pyruvate and succinic semialdehyde [H]

COG id: COG3836

COG function: function code G; 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1788578, Length=250, Percent_Identity=55.6, Blast_Score=288, Evalue=2e-79,
Organism=Escherichia coli, GI1789514, Length=248, Percent_Identity=47.9838709677419, Blast_Score=233, Evalue=1e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR012689
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: 4.1.2.-

Molecular weight: Translated: 28100; Mature: 28100

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGEHAPNNVQTVLTQLQAI
CCCHHHHHHHCCCCCEEEEEECCCCCCHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHH
APYPNQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAREAVRATRYPPAGIRGVGS
CCCCCCCEECCCCCCHHHHHHHHHHCHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHH
ALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQILDVEGVDGVFIGPADLSADM
HHHHHHHCCCCHHHHHHCCCCEEEEEEECHHHHHHCCHHHCCCCCCCEEEECCCCCCCCC
GYAGNPQHPEVQAAIEQAIVQIRESGKAPGILIANEQLAKRYLELGALFVAVGVDTTLLA
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHCCCHHHHH
RAAEALAARFGAQATAVKPGVY
HHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGEHAPNNVQTVLTQLQAI
CCCHHHHHHHCCCCCEEEEEECCCCCCHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHH
APYPNQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAREAVRATRYPPAGIRGVGS
CCCCCCCEECCCCCCHHHHHHHHHHCHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHH
ALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQILDVEGVDGVFIGPADLSADM
HHHHHHHCCCCHHHHHHCCCCEEEEEEECHHHHHHCCHHHCCCCCCCEEEECCCCCCCCC
GYAGNPQHPEVQAAIEQAIVQIRESGKAPGILIANEQLAKRYLELGALFVAVGVDTTLLA
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHCCCHHHHH
RAAEALAARFGAQATAVKPGVY
HHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12384590; 12704152 [H]