Click here to switch to the map view.

The map label for this gene is sitA_2

Identifier: 187733326

GI number: 187733326

Start: 1466164

End: 1467048

Strand: Reverse

Name: sitA_2

Synonym: SbBS512_E1612

Alternate gene names: 187733326

Gene position: 1467048-1466164 (Counterclockwise)

Preceding gene: 187733749

Following gene: 187733024

Centisome position: 31.78

GC content: 48.36

Gene sequence:

>885_bases
TTGGGGTGCCTCGCCCTCACCTGCTCGATCGCATTTCAGGCAAGTGCAACTGAAAAATTCAAGGTCATTACAACATTTAC
CATCATCGCGGATATGGCCAAAAACGTGGCTGGAGATGCTGCAGAAGTCTCATCCATAACCAAGCCTGGTGCAGAAATTC
ATGAGTATCAGCCTACCCCTGGCGATATTAAACGTGCACAGGGGGCACAACTGATTCTCGCCAATGGTATGAATCTGGAA
TTGTGGTTCCAACGCTTTTACCAGCATCTCAATGGGGTTCCAGAAGTAATTGTCTCTTCGGGTGTGACGCCAGTAGGGAT
CACCGAAGGACCCTATGAGGGCAAACCTAATCCCCATGCATGGATGTCGCCAGATAATGCTCTGATTTACGTCGATAATA
TTCGTGATGCGTTGATAAAATACGATCCGGCAAATGCACAAACCTACCAACGTAATGCCGATACTTATAAAGCCAAGATT
ACCCAAACCCTTGCCCCCCTGCGTAAGCAGATTACGGAACTCCCTGAGAATCAGCGATGGATGGTCACCAGTGAAGGGGC
TTTTTCTTATCTCGCACGCGATTTGGGGCTAAAAGAGCTTTATCTGTGGCCGATTAATGCCGATCAACAAGGAACACCGC
AGCAGGTACGTAAGGTTGTTGATATAGTTAAGAAAAATCATATCCCGGCAGTCTTTAGCGAGAGTACGATTTCCGATAAA
CCAGCGCGTCAGGTTGCGCGTGAAACCGGCGCACACTACGGCGGTGTGCTCTATGTCGATTCCCTGAGCACAGAAAACGG
CCCGGTACCTACGTATATCGACCTTCTTAAGGTTACTACCAGTACGCTGGTACAGGGAATTAAAGCCGGGAAAAGGGAGA
AATAA

Upstream 100 bases:

>100_bases
TATAGGTTATGCTATAAAACATAGCTGTTGCTATGTTTGTTCTAATTTTGCTCACTATAGGTACTAAATTATGCACTCAA
TAAAAAAAGTAACCATGCTC

Downstream 100 bases:

>100_bases
TGATGCAATCTGCAGGCATTGTCGTTAATGATGTTACTGTCACCTGGCGTAACGGGCACACAGCACTGCGCGATGCATCC
TTCACAGTACCAAGTGGATC

Product: iron/manganese transport system periplasmic binding protein SitA

Products: Zn (II) [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 294; Mature: 293

Protein sequence:

>294_residues
MGCLALTCSIAFQASATEKFKVITTFTIIADMAKNVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLE
LWFQRFYQHLNGVPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQRNADTYKAKI
TQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINADQQGTPQQVRKVVDIVKKNHIPAVFSESTISDK
PARQVARETGAHYGGVLYVDSLSTENGPVPTYIDLLKVTTSTLVQGIKAGKREK

Sequences:

>Translated_294_residues
MGCLALTCSIAFQASATEKFKVITTFTIIADMAKNVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLE
LWFQRFYQHLNGVPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQRNADTYKAKI
TQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINADQQGTPQQVRKVVDIVKKNHIPAVFSESTISDK
PARQVARETGAHYGGVLYVDSLSTENGPVPTYIDLLKVTTSTLVQGIKAGKREK
>Mature_293_residues
GCLALTCSIAFQASATEKFKVITTFTIIADMAKNVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLEL
WFQRFYQHLNGVPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQRNADTYKAKIT
QTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINADQQGTPQQVRKVVDIVKKNHIPAVFSESTISDKP
ARQVARETGAHYGGVLYVDSLSTENGPVPTYIDLLKVTTSTLVQGIKAGKREK

Specific function: Part of an ATP-driven transport system HI_0359/HI_0360/HI_0361/HI_0362 for iron. Metal-binding component (Potential) [H]

COG id: COG0803

COG function: function code P; ABC-type metal ion transport system, periplasmic component/surface adhesin

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 9 family [H]

Homologues:

Organism=Escherichia coli, GI87081990, Length=251, Percent_Identity=21.5139442231076, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006127
- InterPro:   IPR006129
- InterPro:   IPR006128 [H]

Pfam domain/function: PF01297 SBP_bac_9 [H]

EC number: NA

Molecular weight: Translated: 32321; Mature: 32190

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGCLALTCSIAFQASATEKFKVITTFTIIADMAKNVAGDAAEVSSITKPGAEIHEYQPTP
CCCEEEEEEEEEECCCCCCEEHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHCCCCCC
GDIKRAQGAQLILANGMNLELWFQRFYQHLNGVPEVIVSSGVTPVGITEGPYEGKPNPHA
CHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCCCCCC
WMSPDNALIYVDNIRDALIKYDPANAQTYQRNADTYKAKITQTLAPLRKQITELPENQRW
EECCCCCEEEEECCHHHHEEECCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEE
MVTSEGAFSYLARDLGLKELYLWPINADQQGTPQQVRKVVDIVKKNHIPAVFSESTISDK
EEECCCHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCC
PARQVARETGAHYGGVLYVDSLSTENGPVPTYIDLLKVTTSTLVQGIKAGKREK
HHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
GCLALTCSIAFQASATEKFKVITTFTIIADMAKNVAGDAAEVSSITKPGAEIHEYQPTP
CCEEEEEEEEEECCCCCCEEHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHCCCCCC
GDIKRAQGAQLILANGMNLELWFQRFYQHLNGVPEVIVSSGVTPVGITEGPYEGKPNPHA
CHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCCCCCC
WMSPDNALIYVDNIRDALIKYDPANAQTYQRNADTYKAKITQTLAPLRKQITELPENQRW
EECCCCCEEEEECCHHHHEEECCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEE
MVTSEGAFSYLARDLGLKELYLWPINADQQGTPQQVRKVVDIVKKNHIPAVFSESTISDK
EEECCCHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCC
PARQVARETGAHYGGVLYVDSLSTENGPVPTYIDLLKVTTSTLVQGIKAGKREK
HHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Zn (II) [Periplasm]; H2O; ATP [C]

Specific reaction: Zn (II) [Periplasm] + H2O + ATP = Zn (II) [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 1556062; 10675023 [H]