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The map label for this gene is aaeR

Identifier: 187733277

GI number: 187733277

Start: 3046636

End: 3047565

Strand: Direct

Name: aaeR

Synonym: SbBS512_E3275

Alternate gene names: 187733277

Gene position: 3046636-3047565 (Clockwise)

Preceding gene: 187733514

Following gene: 187730780

Centisome position: 66.0

GC content: 52.69

Gene sequence:

>930_bases
ATGGAACGACTAAAACGCATGTCGGTGTTTGCCAAAGTAGTTGAATTTGGCTCTTTTACCGCCGCCGCCAGACAGCTACA
GATGAGCGTTTCGTCCATCAGTCAGACGGTGTCAAAACTGGAAGATGAGTTGCAGGTAAAGCTGTTAAACCGTAGCACAC
GCAGCATTGGCCTGACCGAAGCCGGTAGAATTTACTACCAGGGCTGCCGTCGTATGCTTCATGAAGTGCAGGATGTTCAT
GAGCAACTGTATGCCTTCAATAACACCCCCATCGGGACGCTACGCATTGGCTGTTCTTCAACTATGGCACAAAATGTTCT
CGCCGGGCTGACAGCCAAAATGCTGAAAGAATACCCAGGTTTGAGCGTCAATCTGGTTACCGGAATTCCAGCCCCCGACC
TGATTGCCGACGGTCTGGATGTGGTGATCCGCGTCGGCGCGTTGCAGGATTCCAGCCTGTTTTCCCGCCGTCTTGGCGCG
ATGCCAATGGTGGTGTGCGCCGCGAAAAGCTATCTCACACAATACGGCATACCGGAAAAACCCGCCGATTTAAGTAGCCA
TTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATTGCACCGGAAGGGATCTCGACTCGCCTGATCC
CGCAAGGGATATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCTAACGGCGGGTGCCGGGATCGCCTACGTGCCG
CTGATGTGGGTGATCAACGAGATCAATCGTGGGGAGCTGGAGATCCTGCTGCCGCGTTATCAGTCAGATCCACGCCCGGT
TTATGCGTTATATACCGAAAAAGATAAGCTGCCGCTGAAGGTACAGGTCGTGATCAACTCGCTGACGGATTATTTTGTTG
AGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAA

Upstream 100 bases:

>100_bases
CAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCATATATTATGTGATCTAAATCA
CTTTTAAGTCAGAGTGAATA

Downstream 100 bases:

>100_bases
TTCATATTGTACTGTTACGTTCTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCCAGAGGAGCGAGGGGACCGT
TCGTGCATGTAGAAAGATTA

Product: putative DNA-binding transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDELQVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVH
EQLYAFNNTPIGTLRIGCSSTMAQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGIFVTNDPMTLVRWLTAGAGIAYVP
LMWVINEINRGELEILLPRYQSDPRPVYALYTEKDKLPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK

Sequences:

>Translated_309_residues
MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDELQVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVH
EQLYAFNNTPIGTLRIGCSSTMAQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGIFVTNDPMTLVRWLTAGAGIAYVP
LMWVINEINRGELEILLPRYQSDPRPVYALYTEKDKLPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK
>Mature_309_residues
MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDELQVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVH
EQLYAFNNTPIGTLRIGCSSTMAQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGIFVTNDPMTLVRWLTAGAGIAYVP
LMWVINEINRGELEILLPRYQSDPRPVYALYTEKDKLPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK

Specific function: Activates transcription of the aaeXAB operon [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789639, Length=309, Percent_Identity=99.6763754045307, Blast_Score=635, Evalue=0.0,
Organism=Escherichia coli, GI145693193, Length=299, Percent_Identity=30.7692307692308, Blast_Score=141, Evalue=5e-35,
Organism=Escherichia coli, GI1786401, Length=288, Percent_Identity=31.5972222222222, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI1787128, Length=304, Percent_Identity=27.3026315789474, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI87081978, Length=298, Percent_Identity=30.2013422818792, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1787589, Length=274, Percent_Identity=28.1021897810219, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI1789440, Length=290, Percent_Identity=27.2413793103448, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI157672245, Length=121, Percent_Identity=33.0578512396694, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1788481, Length=146, Percent_Identity=30.8219178082192, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1789173, Length=250, Percent_Identity=26, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787601, Length=126, Percent_Identity=23.8095238095238, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1788706, Length=141, Percent_Identity=29.7872340425532, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1786508, Length=147, Percent_Identity=27.891156462585, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1788297, Length=123, Percent_Identity=30.0813008130081, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34473; Mature: 34473

Theoretical pI: Translated: 8.04; Mature: 8.04

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDELQVKLLNRSTRSIGLTE
CCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCH
AGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTMAQNVLAGLTAKMLKEYPG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCC
LSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGAMPMVVCAAKSYLTQYGIPEK
CEEEEEECCCCHHHHHCCHHHHEEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCC
PADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGIFVTNDPMTLVRWLTAGAGIAYVP
CCCCCCCCEEEEEECCCCCEEEECCCCCCCEECCCCEEEECCHHHHHHHHHHCCCHHHHH
LMWVINEINRGELEILLPRYQSDPRPVYALYTEKDKLPLKVQVVINSLTDYFVEVGKLFQ
HHHHHHHCCCCCEEEEECCCCCCCCCEEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHH
EMHGRGKEK
HHHCCCCCC
>Mature Secondary Structure
MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDELQVKLLNRSTRSIGLTE
CCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCH
AGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTMAQNVLAGLTAKMLKEYPG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCC
LSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGAMPMVVCAAKSYLTQYGIPEK
CEEEEEECCCCHHHHHCCHHHHEEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCC
PADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGIFVTNDPMTLVRWLTAGAGIAYVP
CCCCCCCCEEEEEECCCCCEEEECCCCCCCEECCCCEEEECCHHHHHHHHHHCCCHHHHH
LMWVINEINRGELEILLPRYQSDPRPVYALYTEKDKLPLKVQVVINSLTDYFVEVGKLFQ
HHHHHHHCCCCCEEEEECCCCCCCCCEEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHH
EMHGRGKEK
HHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]