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The map label for this gene is purU

Identifier: 187732918

GI number: 187732918

Start: 1276674

End: 1277516

Strand: Reverse

Name: purU

Synonym: SbBS512_E1397

Alternate gene names: 187732918

Gene position: 1277516-1276674 (Counterclockwise)

Preceding gene: 187732316

Following gene: 187731932

Centisome position: 27.68

GC content: 47.09

Gene sequence:

>843_bases
ATGCATTCACTCCAACGTAAAGTTCTGCGTACTATTTGTCCGGACCAAAAAGGTCTGATCGCACGTATTACCAATATTTG
CTACAAGCACGAGTTAAATATCGTACAGAACAATGAATTTGTTGATCACCGTACCGGGCGCTTTTTTATGCGCACGGAAC
TGGAAGGGATTTTTAATGATTCCACCCTGCTGGCGGATCTCGATAGCGCATTGCCAGAAGGCTCCGTGCGTGAGCTGAAT
CCTGCCGGTCGTCGCCGGATAGTGATTCTGGTCACTAAAGAAGCGCATTGCCTTGGCGATTTGTTGATGAAAGCCAATTA
CGGCGGCCTGGATGTCGAAATCGCGGCAGTTATTGGTAACCACGATACTTTACGTTCTCTGGTTGAGCGTTTTGATATTC
CGTTTGAGCTGGTAAACCATGAAGGGTTAAGCCGCAACGAGCACGATCAAAAGATGGCAGACGCCATTGATGCTTATAAA
CCTGACTATGTGGTGCTGGCGAAGTATATGCGGGTATTAACGCCGGAATTTGTGGCACGCTTCCCGAATAAAATCATCAA
TATTCACCATTCATTCCTGCCTGCGTTTATCGGCGCACGTCCTTATCACCAGGCCTATGAACGTGGTGTGAAGATTATTG
GCGCAACCGCTCACTATGTGAATGACAATCTGGACGAAGGCCCAATCATCATGCAGGACGTTATTCATGTCGATCATACC
TACACAGCTGAAGATATGATGCGCGCAGGTCGTGACGTCGAGAAAAACGTCTTAAGTCGTGCGCTATACAAAGTACTGGC
ACAGCGCGTCTTTGTTTACGGTAATCGGACGATTATTCTTTAA

Upstream 100 bases:

>100_bases
CCCTGCCCTTGTGGTTCAGGTAAAAAATTTAAAAAGTGCTGCGGCCAATAATGGTTGACGGTACGGTTTAGCAAACACTC
TCAACAAGGTTTTTCCAGCA

Downstream 100 bases:

>100_bases
TCGCCAGCAAAAATAACTGGTTACCTTTAATCCGTTACGGATGAAAATTACGCAACCAGTTCATTTTTCTCAACGTAACA
CTTTACAGCGGCGCGTCATT

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHRTGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELN
PAGRRRIVILVTKEAHCLGDLLMKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVNHEGLSRNEHDQKMADAIDAYK
PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAHYVNDNLDEGPIIMQDVIHVDHT
YTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNRTIIL

Sequences:

>Translated_280_residues
MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHRTGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELN
PAGRRRIVILVTKEAHCLGDLLMKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVNHEGLSRNEHDQKMADAIDAYK
PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAHYVNDNLDEGPIIMQDVIHVDHT
YTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNRTIIL
>Mature_280_residues
MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHRTGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELN
PAGRRRIVILVTKEAHCLGDLLMKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVNHEGLSRNEHDQKMADAIDAYK
PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAHYVNDNLDEGPIIMQDVIHVDHT
YTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNRTIIL

Specific function: Produces formate from formyl-tetrahydrofolate. Provides the major source of formate for the purT-dependent synthesis of 5'-phosphoribosyl-N-formylglycinamide (FGAR) during aerobic growth. Has a role in regulating the one-carbon pool [H]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=190, Percent_Identity=30, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI209869995, Length=190, Percent_Identity=30, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI209869993, Length=190, Percent_Identity=30, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI1787483, Length=280, Percent_Identity=98.9285714285714, Blast_Score=573, Evalue=1e-165,
Organism=Escherichia coli, GI1788846, Length=94, Percent_Identity=42.5531914893617, Blast_Score=87, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17567511, Length=187, Percent_Identity=31.5508021390374, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24582400, Length=159, Percent_Identity=29.559748427673, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF01842 ACT; PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 31948; Mature: 31948

Theoretical pI: Translated: 7.20; Mature: 7.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHRTGRFFMRTELEGIFND
CCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCCCEEEEEHHHHHCCC
STLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLLMKANYGGLDVEIAAVIGN
CHHHHHHHHHCCCCCCHHCCCCCCEEEEEEEECCHHHHHHHHHHCCCCCCCEEEEEEECC
HDTLRSLVERFDIPFELVNHEGLSRNEHDQKMADAIDAYKPDYVVLAKYMRVLTPEFVAR
HHHHHHHHHHHCCCCEECCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHH
FPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAHYVNDNLDEGPIIMQDVIHVDHT
CCHHHHHHHHHHHHHHHCCCCHHHHHHCCCEEEEEEHHHHCCCCCCCCHHHHHHHHHCCC
YTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNRTIIL
CCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEC
>Mature Secondary Structure
MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHRTGRFFMRTELEGIFND
CCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCCCEEEEEHHHHHCCC
STLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLLMKANYGGLDVEIAAVIGN
CHHHHHHHHHCCCCCCHHCCCCCCEEEEEEEECCHHHHHHHHHHCCCCCCCEEEEEEECC
HDTLRSLVERFDIPFELVNHEGLSRNEHDQKMADAIDAYKPDYVVLAKYMRVLTPEFVAR
HHHHHHHHHHHCCCCEECCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHH
FPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAHYVNDNLDEGPIIMQDVIHVDHT
CCHHHHHHHHHHHHHHHCCCCHHHHHHCCCEEEEEEHHHHCCCCCCCCHHHHHHHHHCCC
YTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNRTIIL
CCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]