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The map label for this gene is rnz

Identifier: 187732862

GI number: 187732862

Start: 2446700

End: 2447617

Strand: Direct

Name: rnz

Synonym: SbBS512_E2647

Alternate gene names: 187732862

Gene position: 2446700-2447617 (Clockwise)

Preceding gene: 187732858

Following gene: 187730179

Centisome position: 53.0

GC content: 50.44

Gene sequence:

>918_bases
ATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCA
ACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACC
CTGGAAAACTGGACAAGATTTTTATCAGTCACCTTCATGGCGATCATCTTTTTGGTTTACCCGGCTTGCTGTGCAGTCGT
TCTATGTCAGGCATTATCCAACCCTTAACGATTTATGGTCCGCAAGGTATCCGTGAATTTGTGGAAACCGCGCTGCGGAT
TAGCGGCTCATGGACCGATTATCCGCTGGAAATTGTCGAAATTGGCGCTGGCGAAATTCTCGATGATGGCCTGCGCAAAG
TAACCGCTTATCCGCTGGAACACCCACTGGAATGTTATGGCTATCGTATTGAAGAACATGATAAACCGGGTGCATTAAAT
GCCCAGGCATTAAAAGCTGCTGGCGTGCCGCCTGGCCCACTGTTTCAGGAATTAAAAGCGGGCAAAACAATCATGCTGGA
AGATGGAAGGCAGATTAACGGCGCAGATTACTTAGCTGCTCCAGTGCCAGGTAAAGCGCTCGCTATTTTCGGCGATACCG
GCCCCTGCGATGCCGCACTTGACCTGGCTAAAGGTGTCGATGTCATGGTGCACGAAGCGACGCTGGATATAACCATGGAA
GCCAAAGCCAATAGTCGCGGCCATAGCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGTAAGCTAAT
CATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGA
CTGAACTAGCGAATGATTTCACCGTGTTTAACGTTTAA

Upstream 100 bases:

>100_bases
TAATTCAGAGTGATGCAGATCTTGCCATTCAATCATAATGCTCTCCTTTTAGATGTATGCCTTATACTAGCCGGAATATT
TTTTGAAACGTGATGAACTC

Downstream 100 bases:

>100_bases
CTCTGCTTATTATTAAAAACAGGGCGAAACTTGCCCTGTTATCGCAACCCGCGCTACATATATCCACCCACACGGGCATA
TTGAGCGATTTTTGATATTT

Product: ribonuclease Z

Products: NA

Alternate protein names: RNase Z; tRNA 3 endonuclease; tRNase Z [H]

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQHQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSR
SMSGIIQPLTIYGPQGIREFVETALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
AQALKAAGVPPGPLFQELKAGKTIMLEDGRQINGADYLAAPVPGKALAIFGDTGPCDAALDLAKGVDVMVHEATLDITME
AKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYDDKGCQHLLRECRSIFPATELANDFTVFNV

Sequences:

>Translated_305_residues
MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQHQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSR
SMSGIIQPLTIYGPQGIREFVETALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
AQALKAAGVPPGPLFQELKAGKTIMLEDGRQINGADYLAAPVPGKALAIFGDTGPCDAALDLAKGVDVMVHEATLDITME
AKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYDDKGCQHLLRECRSIFPATELANDFTVFNV
>Mature_305_residues
MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQHQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSR
SMSGIIQPLTIYGPQGIREFVETALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
AQALKAAGVPPGPLFQELKAGKTIMLEDGRQINGADYLAAPVPGKALAIFGDTGPCDAALDLAKGVDVMVHEATLDITME
AKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYDDKGCQHLLRECRSIFPATELANDFTVFNV

Specific function: Zinc phosphodiesterase, which displays some tRNA 3'- processing endonuclease activity. Probably involved in tRNA maturation, by removing a 3'-trailer from precursor tRNA [H]

COG id: COG1234

COG function: function code R; Metal-dependent hydrolases of the beta-lactamase superfamily III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase Z family [H]

Homologues:

Organism=Homo sapiens, GI8922122, Length=361, Percent_Identity=34.6260387811634, Blast_Score=207, Evalue=7e-54,
Organism=Homo sapiens, GI260166619, Length=284, Percent_Identity=27.4647887323944, Blast_Score=95, Evalue=8e-20,
Organism=Homo sapiens, GI145553959, Length=284, Percent_Identity=27.4647887323944, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI260166622, Length=284, Percent_Identity=27.4647887323944, Blast_Score=95, Evalue=1e-19,
Organism=Escherichia coli, GI87082078, Length=305, Percent_Identity=99.672131147541, Blast_Score=625, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71985808, Length=291, Percent_Identity=28.5223367697594, Blast_Score=94, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI71985814, Length=291, Percent_Identity=28.5223367697594, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24652403, Length=175, Percent_Identity=28.5714285714286, Blast_Score=68, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR013471
- InterPro:   IPR013469 [H]

Pfam domain/function: PF00753 Lactamase_B [H]

EC number: =3.1.26.11 [H]

Molecular weight: Translated: 32960; Mature: 32960

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQHQLLHTAFNPGKLDKI
CEEEEEECCCCCCCCCCCEEEEEEEECCCCCCCEEEEECCCCCHHHHHHHCCCCCCHHHH
FISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVETALRISGSWTDYPLEIVE
HHHHHCCCCCCCCHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHCCCCCCCCEEEEE
IGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALNAQALKAAGVPPGPLFQELKA
ECCCHHHHHHHHHHCCCCCCCCHHHHCCEEECCCCCCCCCHHHHHHCCCCCCHHHHHHHC
GKTIMLEDGRQINGADYLAAPVPGKALAIFGDTGPCDAALDLAKGVDVMVHEATLDITME
CCEEEEECCCEECCCCEEECCCCCCEEEEECCCCCCHHHHHHHCCCEEEEEEEEEEEEEE
AKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYDDKGCQHLLRECRSIFPATELANDF
ECCCCCCCCHHHHHHHHHHHCCCCEEEEEEHHCCCCHHHHHHHHHHHHHHCCHHHHCCCE
TVFNV
EEEEC
>Mature Secondary Structure
MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQHQLLHTAFNPGKLDKI
CEEEEEECCCCCCCCCCCEEEEEEEECCCCCCCEEEEECCCCCHHHHHHHCCCCCCHHHH
FISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVETALRISGSWTDYPLEIVE
HHHHHCCCCCCCCHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHCCCCCCCCEEEEE
IGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALNAQALKAAGVPPGPLFQELKA
ECCCHHHHHHHHHHCCCCCCCCHHHHCCEEECCCCCCCCCHHHHHHCCCCCCHHHHHHHC
GKTIMLEDGRQINGADYLAAPVPGKALAIFGDTGPCDAALDLAKGVDVMVHEATLDITME
CCEEEEECCCEECCCCEEECCCCCCEEEEECCCCCCHHHHHHHCCCEEEEEEEEEEEEEE
AKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYDDKGCQHLLRECRSIFPATELANDF
ECCCCCCCCHHHHHHHHHHHCCCCEEEEEEHHCCCCHHHHHHHHHHHHHHCCHHHHCCCE
TVFNV
EEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA