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The map label for this gene is rsmE

Identifier: 187732729

GI number: 187732729

Start: 3144365

End: 3145096

Strand: Direct

Name: rsmE

Synonym: SbBS512_E3378

Alternate gene names: 187732729

Gene position: 3144365-3145096 (Clockwise)

Preceding gene: 187734153

Following gene: 187730682

Centisome position: 68.12

GC content: 53.28

Gene sequence:

>732_bases
ATGCGTATCCCCCGCATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCA
TATCGGGCGCGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCTTTGACGCCGAAA
TTACCAGCGCCAGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGCAAGATCGACGATCGCGAATCTCCGCTGCATATT
CACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACGATCCAGAAATCGATCGAACTCGGTGTAAGCCTCAT
TACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTCCAGCAGTGGCAGAAGA
TTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAACCGGGTGCCGGAAATCCGTCCAGCGATGGATCTGGAAGCCTGGTGT
GCAGAGCAGGATGAAGGACTGAAACTGAATCTTCACCCGCGCGCCAGTAACAGCATCAATACGTTGCCGTTACCGGTTGA
ACGCGTCCGCCTGCTGATTGGCCCGGAAGGCGGTTTATCGGCAGATGAAATTGCCATGACTGCCCGCTATCAATTTACTG
ATATCCTGTTGGGACCTCGCGTTTTGCGTACAGAGACAACTGCGCTCACCGCCATTACCGCGCTACAAGTACGATTTGGC
GATTTGGGCTAA

Upstream 100 bases:

>100_bases
TGCCAGGCGCGAAAGAGCTAACCTACACTAGCGGGACTCTTTTTGTTAACCCCTACCCCACGCGTACAACCGCGTGGGGA
GACGACGCGGATTTTTAACT

Downstream 100 bases:

>100_bases
CGGAGAAGAATAATGATCAAGCTCGGCATCGTGATGGACCCCATCGCAAACATCAACATCAAGAAAGATTCCAGTTTCGC
TATGTTGCTGGAAGCACAGC

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFDGSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHI
HLGQVMSRGEKMEFTIQKSIELGVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLGPRVLRTETTALTAITALQVRFG
DLG

Sequences:

>Translated_243_residues
MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFDGSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHI
HLGQVMSRGEKMEFTIQKSIELGVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLGPRVLRTETTALTAITALQVRFG
DLG
>Mature_243_residues
MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFDGSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHI
HLGQVMSRGEKMEFTIQKSIELGVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLGPRVLRTETTALTAITALQVRFG
DLG

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=243, Percent_Identity=99.5884773662551, Blast_Score=498, Evalue=1e-142,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 26979; Mature: 26979

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFDGSNQVFDAEITSASKK
CCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEHHHCCCCCC
SVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIELGVSLITPLFSERCGVKLD
CEEEEEEECCCCCCCCCEEEEHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCEEC
SERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWCAEQDEGLKLNLHPRASNSIN
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCCCEEEECCCCCCCCC
TLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLGPRVLRTETTALTAITALQVRFG
CCCCCHHHEEEEECCCCCCCHHHEEEEEEHHHHHHHHCCEEEHHHHHHHHHHHHEEEEEC
DLG
CCC
>Mature Secondary Structure
MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFDGSNQVFDAEITSASKK
CCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEHHHCCCCCC
SVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIELGVSLITPLFSERCGVKLD
CEEEEEEECCCCCCCCCEEEEHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCEEC
SERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWCAEQDEGLKLNLHPRASNSIN
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCCCEEEECCCCCCCCC
TLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLGPRVLRTETTALTAITALQVRFG
CCCCCHHHEEEEECCCCCCCHHHEEEEEEHHHHHHHHCCEEEHHHHHHHHHHHHEEEEEC
DLG
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]