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The map label for this gene is yliE

Identifier: 187732702

GI number: 187732702

Start: 2310128

End: 2310673

Strand: Reverse

Name: yliE

Synonym: SbBS512_E2512

Alternate gene names: 187732702

Gene position: 2310673-2310128 (Counterclockwise)

Preceding gene: 187732071

Following gene: 187730261

Centisome position: 50.06

GC content: 43.59

Gene sequence:

>546_bases
ATGGGTGAATTCAGACATTTTAGTCAGCGTGCGTCGCTGAAGGATGATTTCTTACTGCATATTAATGTTTCGCCCTGGCA
GTTAAACGAACCGCACTTTCATGAGCGTTTTACCACCATCATGAAAGAAAATGGCCTGAAGGCGAACAGCCTCTGTGTTG
AGATCACTGAAACCGTGATCGAGCGAATTAATGAACATTTTTATCTCAATATTGAACAACTGCGTAAACAAGGGGTACGG
ATATCGATTGATGACTTTGGCACCGGTTTGTCAAACCTGAAACGTTTTTATGAAATTAATCCAGACAGCATAAAGGTGGA
CTCGCAATTTACCGGCGATATTTTCGGTACTGCGGGAAAAATTGTGCGCATTATTTTCGATCTGGCACGCTATAACCGGA
TCCCGGTGATTGCGGAAGGCGTAGAGAGCGAAGACGTTGCGCGCGAATTAATTAAATTAGGATGTGTTCAGGCTCAGGGG
TATCTGTACCAGAAACCTATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAG

Upstream 100 bases:

>100_bases
AAATCGATACTGGAAAAACTCTAACTCGGTGGCCAGTTTTTGTTGACCACTTCATATCGGGTTTATCAATGAGCTGGGTT
ATCAGATTATTAAAACGGCA

Downstream 100 bases:

>100_bases
TTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCG
GGATCTACACTCAAATGGTA

Product: putative cyclic diguanylate phosphodiesterase (EAL) domain

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 181; Mature: 180

Protein sequence:

>181_residues
MGEFRHFSQRASLKDDFLLHINVSPWQLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVR
ISIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAEGVESEDVARELIKLGCVQAQG
YLYQKPMPFSAWDKSGKLVKE

Sequences:

>Translated_181_residues
MGEFRHFSQRASLKDDFLLHINVSPWQLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVR
ISIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAEGVESEDVARELIKLGCVQAQG
YLYQKPMPFSAWDKSGKLVKE
>Mature_180_residues
GEFRHFSQRASLKDDFLLHINVSPWQLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRI
SIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAEGVESEDVARELIKLGCVQAQGY
LYQKPMPFSAWDKSGKLVKE

Specific function: Overexpression reduces biofilm formation. Cyclic-di-GMP is a second messenger which controls cell surface-associated traits in bacteria

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 EAL domain

Homologues:

Organism=Escherichia coli, GI1787055, Length=181, Percent_Identity=100, Blast_Score=380, Evalue=1e-107,
Organism=Escherichia coli, GI87082096, Length=152, Percent_Identity=37.5, Blast_Score=92, Evalue=2e-20,
Organism=Escherichia coli, GI1788849, Length=151, Percent_Identity=31.7880794701987, Blast_Score=84, Evalue=6e-18,
Organism=Escherichia coli, GI1787541, Length=156, Percent_Identity=32.6923076923077, Blast_Score=82, Evalue=2e-17,
Organism=Escherichia coli, GI226510982, Length=162, Percent_Identity=32.0987654320988, Blast_Score=80, Evalue=7e-17,
Organism=Escherichia coli, GI87081921, Length=153, Percent_Identity=31.3725490196078, Blast_Score=80, Evalue=7e-17,
Organism=Escherichia coli, GI87081980, Length=170, Percent_Identity=31.1764705882353, Blast_Score=74, Evalue=5e-15,
Organism=Escherichia coli, GI1786507, Length=158, Percent_Identity=29.746835443038, Blast_Score=70, Evalue=7e-14,
Organism=Escherichia coli, GI87081845, Length=161, Percent_Identity=28.5714285714286, Blast_Score=70, Evalue=8e-14,
Organism=Escherichia coli, GI1790496, Length=181, Percent_Identity=28.1767955801105, Blast_Score=69, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YLIE_ECOLI (P75800)

Other databases:

- EMBL:   U00096
- EMBL:   AP009048
- PIR:   A64821
- RefSeq:   AP_001464.1
- RefSeq:   NP_415354.1
- ProteinModelPortal:   P75800
- SMR:   P75800
- STRING:   P75800
- EnsemblBacteria:   EBESCT00000004102
- EnsemblBacteria:   EBESCT00000016335
- GeneID:   945462
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW0817
- KEGG:   eco:b0833
- EchoBASE:   EB3249
- EcoGene:   EG13476
- eggNOG:   COG2200
- GeneTree:   EBGT00070000032051
- HOGENOM:   HBG467594
- OMA:   GFLLHIN
- ProtClustDB:   CLSK879780
- BioCyc:   EcoCyc:G6433-MONOMER
- Genevestigator:   P75800
- InterPro:   IPR001633
- SMART:   SM00052

Pfam domain/function: PF00563 EAL

EC number: =3.1.4.52

Molecular weight: Translated: 20867; Mature: 20736

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS50883 EAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGEFRHFSQRASLKDDFLLHINVSPWQLNEPHFHERFTTIMKENGLKANSLCVEITETVI
CCCCHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
ERINEHFYLNIEQLRKQGVRISIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGK
HHCCHHEEEEHHHHHHCCCEEEEHHHCCCHHHHHHHHCCCCCCEEECCCCCCCHHHHHHH
IVRIIFDLARYNRIPVIAEGVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVK
HHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCC
E
C
>Mature Secondary Structure 
GEFRHFSQRASLKDDFLLHINVSPWQLNEPHFHERFTTIMKENGLKANSLCVEITETVI
CCCHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
ERINEHFYLNIEQLRKQGVRISIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGK
HHCCHHEEEEHHHHHHCCCEEEEHHHCCCHHHHHHHHCCCCCCEEECCCCCCCHHHHHHH
IVRIIFDLARYNRIPVIAEGVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVK
HHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCC
E
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503