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The map label for this gene is hyfC

Identifier: 187732400

GI number: 187732400

Start: 2652320

End: 2653264

Strand: Direct

Name: hyfC

Synonym: SbBS512_E2855

Alternate gene names: 187732400

Gene position: 2652320-2653264 (Clockwise)

Preceding gene: 187730471

Following gene: 187731081

Centisome position: 57.46

GC content: 53.86

Gene sequence:

>945_bases
ATGAGACAAACTCTTTGCGACGAATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGCTAACCCCGCTTTT
TACGGGTATTTCCCAGCAGATACGCGCGCGTATGCACTCCCGCCGCGGGCCGGGGATCTGGCAGGATTATCGCGAGATCC
ACAAACTGTTTAAACGCCAGGAAGTTGCGCCTATATCTTCAGGTCTGATGTTCCGCCTGATGCCGTGGGTATTAATCAGC
AGCATGCTGGTGCTGGCGATGGCCCTACCACTTTTTATTACCGTTTCCCCTTTTGCGGGCGGCGGCGATCTGATCACCCT
TATCTATCTTCTTGCCCTGTTTCGTTTTTTCTTTGCTCTTTCCGGGCTGGATACCGGAAGCCCGTTTGCGGGAGTCGGTG
CCAGTCGCGAGTTGACGCTCGGCATTCTGGTCGAACCCATGCTCATTCTCTCACTGCTGGTATTGGCGCTGATAGCAGGT
TCCACGCATATCGAGATGATCAGCAATACGCTGGCAACGGGCTGGAACTCGCCGCTAACCACCGTACTGGCATTACTGGC
CTGTGGTTTTGCCTGCTTCATTGAGATGGGAAAAATTCCCTTTGATGTTGCTGAAGCAGAACAGGAATTACAGGAAGGCC
CGCTGACCGAATATTCCGGTGCCGGGCTGGCGCTAGCGAAATGGGGGCTGGGGCTGAAACAGGTCGTGATGGCAGCACTG
TTTGTGGCCCTGTTTCTGCCCTTTGGGCGCGCGCAAGAACTTTCTCTCGCCTGCCTGCTGACTTCACTTGTCGTTACGCT
GCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCAATCGCAGAAAACACGCTGGCACGCGGGCGTTTTTTACTCATTCACC
ATGTGACCTGGCTTGGCTTCAGCCTTGCTGCGCTTGCATGGGTCTTCTGGTTAACCGGTCTGTAA

Upstream 100 bases:

>100_bases
ATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATCTAGTTGCCGCACTAGTTGTGCTGCTAATCGCTAT
TGCCGTCTAAGGAAATCACC

Downstream 100 bases:

>100_bases
GGAGCACTGACGGAATATGGAAAATCTTGCTCTGACGACGTTAATGCTGCCTTTTATCGGCGCACTGGTCGTTTCGTTTT
CGCCACAACGTCGGGCCGCC

Product: hydrogenase-4 component C

Products: H2; oxidized ferredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MRQTLCDEYLVIFALAQAVILLLTPLFTGISQQIRARMHSRRGPGIWQDYREIHKLFKRQEVAPISSGLMFRLMPWVLIS
SMLVLAMALPLFITVSPFAGGGDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIAG
STHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTEYSGAGLALAKWGLGLKQVVMAAL
FVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFVLASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL

Sequences:

>Translated_314_residues
MRQTLCDEYLVIFALAQAVILLLTPLFTGISQQIRARMHSRRGPGIWQDYREIHKLFKRQEVAPISSGLMFRLMPWVLIS
SMLVLAMALPLFITVSPFAGGGDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIAG
STHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTEYSGAGLALAKWGLGLKQVVMAAL
FVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFVLASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL
>Mature_314_residues
MRQTLCDEYLVIFALAQAVILLLTPLFTGISQQIRARMHSRRGPGIWQDYREIHKLFKRQEVAPISSGLMFRLMPWVLIS
SMLVLAMALPLFITVSPFAGGGDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIAG
STHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTEYSGAGLALAKWGLGLKQVVMAAL
FVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFVLASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL

Specific function: Unknown

COG id: COG0650

COG function: function code C; Formate hydrogenlyase subunit 4

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 1 family [H]

Homologues:

Organism=Escherichia coli, GI87082115, Length=315, Percent_Identity=97.7777777777778, Blast_Score=553, Evalue=1e-159,
Organism=Escherichia coli, GI1789077, Length=303, Percent_Identity=51.4851485148515, Blast_Score=302, Evalue=2e-83,
Organism=Escherichia coli, GI1788618, Length=305, Percent_Identity=27.5409836065574, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001694
- InterPro:   IPR018086 [H]

Pfam domain/function: PF00146 NADHdh [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 34253; Mature: 34253

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: PS00668 COMPLEX1_ND1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQTLCDEYLVIFALAQAVILLLTPLFTGISQQIRARMHSRRGPGIWQDYREIHKLFKRQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
EVAPISSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGGGDLITLIYLLALFRFFFAL
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHH
SGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIAGSTHIEMISNTLATGWNSPLT
HCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHH
TVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTEYSGAGLALAKWGLGLKQVVMAAL
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCHHHHHHHHH
FVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFVLASIAENTLARGRFLLIHHVTWLGF
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
SLAALAWVFWLTGL
HHHHHHHHHHHHCC
>Mature Secondary Structure
MRQTLCDEYLVIFALAQAVILLLTPLFTGISQQIRARMHSRRGPGIWQDYREIHKLFKRQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
EVAPISSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGGGDLITLIYLLALFRFFFAL
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHH
SGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIAGSTHIEMISNTLATGWNSPLT
HCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHH
TVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTEYSGAGLALAKWGLGLKQVVMAAL
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCHHHHHHHHH
FVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFVLASIAENTLARGRFLLIHHVTWLGF
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
SLAALAWVFWLTGL
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]