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The map label for this gene is cysJ

Identifier: 187732267

GI number: 187732267

Start: 2906360

End: 2908159

Strand: Direct

Name: cysJ

Synonym: SbBS512_E3111

Alternate gene names: 187732267

Gene position: 2906360-2908159 (Clockwise)

Preceding gene: 187732080

Following gene: 187732880

Centisome position: 62.96

GC content: 56.5

Gene sequence:

>1800_bases
ATGACGACACAGGTCCCACCTTCCGCGTTGCTTCCGTTGAACCCGGAGCAACTGGCACGCCTTCAGGCGGCCACGACCGA
TTTAACGCCTACCCAGCTTGCCTGGGTTTCTGGCTATTTCTGGGGCGTACTCAATCAGCAGCCTGCTGCGCTTGCAGCGA
CGCCAGCGCCAGCCGCAGAAATGCCGGGTATAACTATTATCTCCGCCTCGCAAACCGGCAATGCGCGCCGGGTTGCTGAA
GCATTACGCGATGATTTATTAGCAGCAAAACTGAACGTTAAGCTGGTGAACGCGGGCGACTATAAATTCAAACAAATCGC
CAGCGAAAAACTGCTCATCGTAGTGACGTCAACGCAAGGGGAAGGGGAACCGCCGGAAGAAGCCGTCGCGCTGCATAAGT
TCCTGTTCTCCAAAAAAGCGCCAAAGCTGGAAAACACCGCGTTTGCCGTGTTTAGCCTCGGCGATAGCTCTTATGAATTT
TTCTGCCAGTCCGGGAAAGATTTCGACAGCAAGCTGGCGGAACTGGGTGGTGAACGCCTGCTCGACCGTGTCGATGCCGA
TGTTGAATACCAGGCTGCTGCCAGCGAGTGGCGCGCCCGCGTGGTTGATGCGCTTAAATCGCGTGCGCCTGTCGCGGCAC
CTTCGCAATCCGTCGCTACTGGCGCGGTAAATGAAATCCACGCCAGCCCGTACAGCAAAGACGCGCCGCTGGTGGCTAGC
CTCTCTGTTAACCAGAAAATTACCGGGCGTAACTCTGAAAAAGACGTTCGCCATATCGAAATTGACTTAGGTGACTCGGG
CCTGCGTTACCAGCCGGGTGACGCGCTGGGCGTCTGGTATCAGAACGATCCGGCACTGGTGAAAGAACTTGTCGAACTGC
TGTGGCTGAAAGGCGATGAACCTGTCACCGTCGAGGGCAAAACGTTGCCTCTGAACGAAGCGCTACAGTGGCACTTCGAA
CTGACCGTCAACACCGCCAACATTGTTGAGAATTACGCCACGCTTACCCGCAGCGAAACACTGCTGCCGCTGGTGGGCGA
TAAAGCGAAGTTACAGCATTACGCCGCGACGACGCCGATTGTCGACATGGTGCGTTTCTCTCCGGCGCAACTGGATGCCG
AAGCGCTGATTAATCTGCTGCGCCCGCTGACCCCGCGCCTGTATTCCATCGCCACCTCGCAGGCGGAAGTCGAGAACGAA
GTACACGTCACCGTTGGTGTGGTGCGTTACGACGTGGAAGGCCGAGCCCGTGCCGGTGGTGCCTCCAGCTTCCTCGCGGA
TCGCGTGGAAGAAGAGGGCGAAGTCCGCGTATTTATCGAACATAACGATAACTTTCGCCTGCCCGCTAACCCGGAAACCC
CGGTGATTATGATTGGCCCAGGCACCGGTATTGCGCCGTTCCGCGCCTTTATGCAGCAACGCGCCGCCGACGAAGCGCCA
GGTAAAAACTGGCTGTTCTTTGGTAATCCGCACTTTACGGAAGACTTCCTGTACCAGGTGGAGTGGCAGCGCTACGTCAA
AGATGGCGTGCTGACACGTATCGATCTTGCCTGGTCGCGCGACCAAAAAGAAAAAGTTTACGTACAAGACAAACTGCGCG
AACAGGGCGCGGAGCTGTGGCGCTGGATCAATGATGGTGCCCACATTTATGTCTGCGGCGACGCTAATCGCATGGCGAAA
GACGTTGAGCAGGCACTTCTGGAAGTGATTGCCGAATTTGGTGGCATGGACACCGAAGCGGCGGATGAATTTTTAAGTGA
GCTGCGCGTAGAGCGCCGTTATCAGCGAGATGTCTACTAA

Upstream 100 bases:

>100_bases
TCTAACTCGGTGGCCAGTTTTTGTTGACCACTTCACTCTTCTGTTTTATGGGCGCTGACAGGGCGCAGAAACAGCTTTGC
TTACTGGAACATAACGACGC

Downstream 100 bases:

>100_bases
TGAGCGAAAAACATCCAGGGCCTTTAGTGGTCGAAGGAAAACTGACAGACGCCGAGCGCATGAAGCTTGAAAGCAACTAC
CTGCGCGGCACCATTGCGGA

Product: sulfite reductase subunit alpha

Products: NA

Alternate protein names: SiR-FP [H]

Number of amino acids: Translated: 599; Mature: 598

Protein sequence:

>599_residues
MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVLNQQPAALAATPAPAAEMPGITIISASQTGNARRVAE
ALRDDLLAAKLNVKLVNAGDYKFKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSVATGAVNEIHASPYSKDAPLVAS
LSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDALGVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFE
LTVNTANIVENYATLTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIATSQAEVENE
VHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPANPETPVIMIGPGTGIAPFRAFMQQRAADEAP
GKNWLFFGNPHFTEDFLYQVEWQRYVKDGVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAK
DVEQALLEVIAEFGGMDTEAADEFLSELRVERRYQRDVY

Sequences:

>Translated_599_residues
MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVLNQQPAALAATPAPAAEMPGITIISASQTGNARRVAE
ALRDDLLAAKLNVKLVNAGDYKFKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSVATGAVNEIHASPYSKDAPLVAS
LSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDALGVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFE
LTVNTANIVENYATLTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIATSQAEVENE
VHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPANPETPVIMIGPGTGIAPFRAFMQQRAADEAP
GKNWLFFGNPHFTEDFLYQVEWQRYVKDGVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAK
DVEQALLEVIAEFGGMDTEAADEFLSELRVERRYQRDVY
>Mature_598_residues
TTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVLNQQPAALAATPAPAAEMPGITIISASQTGNARRVAEA
LRDDLLAAKLNVKLVNAGDYKFKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEFF
CQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSVATGAVNEIHASPYSKDAPLVASL
SVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDALGVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFEL
TVNTANIVENYATLTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIATSQAEVENEV
HVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPANPETPVIMIGPGTGIAPFRAFMQQRAADEAPG
KNWLFFGNPHFTEDFLYQVEWQRYVKDGVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKD
VEQALLEVIAEFGGMDTEAADEFLSELRVERRYQRDVY

Specific function: Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L- cysteine from sulfate. The flavoprotein component catalyzes the electron flow f

COG id: COG0369

COG function: function code P; Sulfite reductase, alpha subunit (flavoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 flavodoxin-like domain [H]

Homologues:

Organism=Homo sapiens, GI127139033, Length=601, Percent_Identity=30.4492512479201, Blast_Score=244, Evalue=2e-64,
Organism=Homo sapiens, GI7657393, Length=601, Percent_Identity=29.783693843594, Blast_Score=230, Evalue=2e-60,
Organism=Homo sapiens, GI221316709, Length=601, Percent_Identity=29.783693843594, Blast_Score=225, Evalue=7e-59,
Organism=Homo sapiens, GI221316705, Length=610, Percent_Identity=29.344262295082, Blast_Score=223, Evalue=3e-58,
Organism=Homo sapiens, GI24041029, Length=597, Percent_Identity=30.6532663316583, Blast_Score=222, Evalue=8e-58,
Organism=Homo sapiens, GI10835173, Length=651, Percent_Identity=29.4930875576037, Blast_Score=202, Evalue=7e-52,
Organism=Homo sapiens, GI221316707, Length=531, Percent_Identity=29.7551789077213, Blast_Score=200, Evalue=4e-51,
Organism=Homo sapiens, GI40254422, Length=649, Percent_Identity=27.2727272727273, Blast_Score=166, Evalue=4e-41,
Organism=Homo sapiens, GI169790956, Length=437, Percent_Identity=28.3752860411899, Blast_Score=160, Evalue=4e-39,
Organism=Homo sapiens, GI169790958, Length=437, Percent_Identity=28.3752860411899, Blast_Score=160, Evalue=4e-39,
Organism=Escherichia coli, GI1789123, Length=599, Percent_Identity=99.4991652754591, Blast_Score=1222, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17554134, Length=636, Percent_Identity=29.2452830188679, Blast_Score=235, Evalue=6e-62,
Organism=Caenorhabditis elegans, GI17566446, Length=579, Percent_Identity=25.73402417962, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17531441, Length=394, Percent_Identity=24.8730964467005, Blast_Score=94, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6321832, Length=636, Percent_Identity=28.1446540880503, Blast_Score=210, Evalue=5e-55,
Organism=Saccharomyces cerevisiae, GI6321143, Length=397, Percent_Identity=31.7380352644836, Blast_Score=163, Evalue=6e-41,
Organism=Saccharomyces cerevisiae, GI6325305, Length=626, Percent_Identity=22.8434504792332, Blast_Score=129, Evalue=1e-30,
Organism=Drosophila melanogaster, GI17137192, Length=624, Percent_Identity=28.0448717948718, Blast_Score=216, Evalue=4e-56,
Organism=Drosophila melanogaster, GI24582192, Length=510, Percent_Identity=29.8039215686275, Blast_Score=198, Evalue=1e-50,
Organism=Drosophila melanogaster, GI78706872, Length=524, Percent_Identity=32.4427480916031, Blast_Score=186, Evalue=4e-47,
Organism=Drosophila melanogaster, GI24583543, Length=524, Percent_Identity=32.4427480916031, Blast_Score=185, Evalue=7e-47,
Organism=Drosophila melanogaster, GI78706876, Length=524, Percent_Identity=32.4427480916031, Blast_Score=185, Evalue=7e-47,
Organism=Drosophila melanogaster, GI24660907, Length=595, Percent_Identity=26.0504201680672, Blast_Score=157, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24660903, Length=595, Percent_Identity=26.0504201680672, Blast_Score=157, Evalue=2e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010199
- InterPro:   IPR003097
- InterPro:   IPR017927
- InterPro:   IPR001094
- InterPro:   IPR008254
- InterPro:   IPR001709
- InterPro:   IPR023173
- InterPro:   IPR001433
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00667 FAD_binding_1; PF00258 Flavodoxin_1; PF00175 NAD_binding_1 [H]

EC number: =1.8.1.2 [H]

Molecular weight: Translated: 66236; Mature: 66105

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS50902 FLAVODOXIN_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVLNQQPAALAATPAPAAE
CCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCHHH
MPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYKFKQIASEKLLIVVTSTQG
CCCEEEEECCCCCCHHHHHHHHHHHHHEEEEEEEEEECCCCHHHHHCCCCEEEEEECCCC
EGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEFFCQSGKDFDSKLAELGGERL
CCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHHCCCCCCHHHHHHHHCHHHH
LDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSVATGAVNEIHASPYSKDAPLVAS
HHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCHHHHCCCCCCCCCCEEEE
LSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDALGVWYQNDPALVKELVELLWLKGDE
EECCCEECCCCCCCCCEEEEEEECCCCCEECCCCEEEEEECCCHHHHHHHHHHHHCCCCC
PVTVEGKTLPLNEALQWHFELTVNTANIVENYATLTRSETLLPLVGDKAKLQHYAATTPI
CEEECCCCCCCCCCEEEEEEEEEEHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHCCCCH
VDMVRFSPAQLDAEALINLLRPLTPRLYSIATSQAEVENEVHVTVGVVRYDVEGRARAGG
HHHHHCCCHHCCHHHHHHHHHCCCHHHHHHHCCHHHHCCCEEEEEEEEEEECCCCCCCCC
ASSFLADRVEEEGEVRVFIEHNDNFRLPANPETPVIMIGPGTGIAPFRAFMQQRAADEAP
HHHHHHHHHCCCCCEEEEEEECCCEECCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCC
GKNWLFFGNPHFTEDFLYQVEWQRYVKDGVLTRIDLAWSRDQKEKVYVQDKLREQGAELW
CCCEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHH
RWINDGAHIYVCGDANRMAKDVEQALLEVIAEFGGMDTEAADEFLSELRVERRYQRDVY
HHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVLNQQPAALAATPAPAAE
CCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCHHH
MPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYKFKQIASEKLLIVVTSTQG
CCCEEEEECCCCCCHHHHHHHHHHHHHEEEEEEEEEECCCCHHHHHCCCCEEEEEECCCC
EGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEFFCQSGKDFDSKLAELGGERL
CCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHHCCCCCCHHHHHHHHCHHHH
LDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSVATGAVNEIHASPYSKDAPLVAS
HHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCHHHHCCCCCCCCCCEEEE
LSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDALGVWYQNDPALVKELVELLWLKGDE
EECCCEECCCCCCCCCEEEEEEECCCCCEECCCCEEEEEECCCHHHHHHHHHHHHCCCCC
PVTVEGKTLPLNEALQWHFELTVNTANIVENYATLTRSETLLPLVGDKAKLQHYAATTPI
CEEECCCCCCCCCCEEEEEEEEEEHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHCCCCH
VDMVRFSPAQLDAEALINLLRPLTPRLYSIATSQAEVENEVHVTVGVVRYDVEGRARAGG
HHHHHCCCHHCCHHHHHHHHHCCCHHHHHHHCCHHHHCCCEEEEEEEEEEECCCCCCCCC
ASSFLADRVEEEGEVRVFIEHNDNFRLPANPETPVIMIGPGTGIAPFRAFMQQRAADEAP
HHHHHHHHHCCCCCEEEEEEECCCEECCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCC
GKNWLFFGNPHFTEDFLYQVEWQRYVKDGVLTRIDLAWSRDQKEKVYVQDKLREQGAELW
CCCEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHH
RWINDGAHIYVCGDANRMAKDVEQALLEVIAEFGGMDTEAADEFLSELRVERRYQRDVY
HHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA