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The map label for this gene is envZ

Identifier: 187732077

GI number: 187732077

Start: 3508850

End: 3510202

Strand: Reverse

Name: envZ

Synonym: SbBS512_E3783

Alternate gene names: 187732077

Gene position: 3510202-3508850 (Counterclockwise)

Preceding gene: 187731609

Following gene: 187734214

Centisome position: 76.04

GC content: 56.91

Gene sequence:

>1353_bases
ATGAGGCGATTGCGCTTCTCGCCACGAAGTTCATTTGCCCGTACGTTATTGCTCATCGTCACCTTGCTGTTCGCCAGCCT
GGTGACGACTTATCTGGTGGTGCTGAACTTCGCGATTTTGCCGAGCCTCCAGCAGTTTAATAAAGTCCTCGCGTACGAAG
TGCGTATGTTGATGACCGACAAACTGCAACTGGAGGACGGCACGCAGTTGGTTGTGCCTCCCGCTTTCCGTCGGGAGATC
TACCGTGAGCTGGGGATCTCTCTCTACTCCAACGAGGCTGCCGAAGAGGCAGGTCTGCGTTGGGCGCAACACTATGAATT
CTTAAGCCATCAGATGGCACAGCAACTGGGCGGCCCGACGGAAGTGCGCGTTGAGGTCAACAAAAGTTCGCCTGTCGTCT
GGCTGAAAACCTGGCTGTCGCCCAATATCTGGGTACGCGTGCCGCTGACCGAAATTCATCAGGGCGATTTCTCTCCGCTG
TTCCGCTATACGCTGGCGATTATGCTATTGGCGATAGGCGGGGCGTGGCTGTTTATTCGTATCCAGAACCGACCGTTGGT
CGATCTCGAACATGCTGCTTTGCAGGTCGGTAAAGGGATTATTCCACCGCCGCTGCGTGAATATGGCGCGTCCGAGGTAC
GTTCGGTTACCCGCGCCTTTAACCATATGGCGGCTGGTGTTAAGCAACTGGCGGATGACCGCACGTTGCTGATGGCGGGG
GTAAGTCATGACTTACGCACGCCGCTGACGCGTATTCGCCTGGCGACCGAGATGATGAGCGAACAGGATGGCTACCTGGC
CGAATCGATCAATAAAGATATCGAAGAGTGCAACGCCATCATTGAGCAGTTTATCGACTACCTGCGCACCGGGCAGGAGA
TGCCGATGGAAATGGCGGATCTCAACGCAGTACTCGGTGAGGTTATTGCTGCCGAAAGTGGCTATGAGCGGGAAATTGAA
ACCGCGCTTTACCCCGGCAGCATTGAAGTGAAAATGCACCCGCTGTCGATCAAACGCGCGGTGGCGAATATGGTGGTCAA
CGCCGCCCGTTACGGCAATGGCTGGATCAAACTCAGCAGCGGGACGGAGCCGAATCGCGCCTGGTTCCAGGTGGAAGATG
ACGGTCCGGGAATTGCGCCGGAACAACGTAAGCACCTGTTCCAGCCGTTTGTCCGCGGCGACAGTGCACGCACCATTAGC
GGCACGGGATTAGGGCTGGCAATTGTGCAGCGTATCGTGGATAACCATAACGGGATGCTGGAGCTTGGCACCAGCGAGCG
GGGCGGGCTTTCCATTCGCGCCTGGCTGCCAGTGCCGGTAACGCGGGCGCAGGGCACGACAAAAGAAGGGTAA

Upstream 100 bases:

>100_bases
CGCGTCTGCGCCGCATGGTAGAAGAAGATCCAGCGCATCCGCGTTACATTCAGACCGTTTGGGGCCTGGGCTACGTCTTT
GTACCGGACGGCTCTAAAGC

Downstream 100 bases:

>100_bases
ATAAACGGGAGGCGAAGGTGCCTCCCGTTTTGCTTTCTATAAGATACTGGATAGATATTCTCCAGCTTCAAATCATTACA
GTTTCGGACCAGCCGCTACC

Product: osmolarity sensor protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQQFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREI
YRELGISLYSNEAAEEAGLRWAQHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTRAFNHMAAGVKQLADDRTLLMAG
VSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEECNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIE
TALYPGSIEVKMHPLSIKRAVANMVVNAARYGNGWIKLSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVRGDSARTIS
GTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG

Sequences:

>Translated_450_residues
MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQQFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREI
YRELGISLYSNEAAEEAGLRWAQHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTRAFNHMAAGVKQLADDRTLLMAG
VSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEECNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIE
TALYPGSIEVKMHPLSIKRAVANMVVNAARYGNGWIKLSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVRGDSARTIS
GTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
>Mature_450_residues
MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQQFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREI
YRELGISLYSNEAAEEAGLRWAQHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTRAFNHMAAGVKQLADDRTLLMAG
VSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEECNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIE
TALYPGSIEVKMHPLSIKRAVANMVVNAARYGNGWIKLSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVRGDSARTIS
GTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG

Specific function: Member of the two-component regulatory system envZ/ompR involved in the regulation of osmoregulation (genes ompF and ompC). EnvZ functions as a membrane-associated protein kinase that phosphorylates ompR in response to environmental signals [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1789808, Length=450, Percent_Identity=99.7777777777778, Blast_Score=920, Evalue=0.0,
Organism=Escherichia coli, GI1787894, Length=277, Percent_Identity=32.4909747292419, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1790346, Length=274, Percent_Identity=29.9270072992701, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1790436, Length=218, Percent_Identity=26.1467889908257, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1790551, Length=270, Percent_Identity=28.1481481481481, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI87082128, Length=291, Percent_Identity=24.7422680412371, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1786912, Length=217, Percent_Identity=29.0322580645161, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1786600, Length=214, Percent_Identity=30.8411214953271, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1786783, Length=283, Percent_Identity=27.208480565371, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1788549, Length=218, Percent_Identity=27.5229357798165, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1788393, Length=247, Percent_Identity=27.9352226720648, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1789403, Length=249, Percent_Identity=27.3092369477912, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790861, Length=240, Percent_Identity=25.4166666666667, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1789149, Length=210, Percent_Identity=30.4761904761905, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI87081816, Length=279, Percent_Identity=26.5232974910394, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI145693157, Length=251, Percent_Identity=23.9043824701195, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1787374, Length=232, Percent_Identity=26.2931034482759, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 50349; Mature: 50349

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQQFNKVLAYEVRMLMTD
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHC
KLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWAQHYEFLSHQMAQQLGGPT
CEEECCCCEEEECHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
EVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPLFRYTLAIMLLAIGGAWLFIR
EEEEEECCCCCEEEEEECCCCCCEEEECHHHHCCCCCHHHHHHHHHHHHHHHCCEEEEEE
IQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTRAFNHMAAGVKQLADDRTLLMAG
ECCCCCCHHHHHHHHHCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEC
VSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEECNAIIEQFIDYLRTGQEMPMEMAD
CCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
LNAVLGEVIAAESGYEREIETALYPGSIEVKMHPLSIKRAVANMVVNAARYGNGWIKLSS
HHHHHHHHHHCCCCCCHHHHHEECCCEEEEEECCHHHHHHHHHHHHHHHHCCCCEEEECC
GTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVRGDSARTISGTGLGLAIVQRIVDNHNGML
CCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHCCCCEE
ELGTSERGGLSIRAWLPVPVTRAQGTTKEG
EECCCCCCCEEEEEEECCCEECCCCCCCCC
>Mature Secondary Structure
MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQQFNKVLAYEVRMLMTD
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHC
KLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWAQHYEFLSHQMAQQLGGPT
CEEECCCCEEEECHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
EVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPLFRYTLAIMLLAIGGAWLFIR
EEEEEECCCCCEEEEEECCCCCCEEEECHHHHCCCCCHHHHHHHHHHHHHHHCCEEEEEE
IQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTRAFNHMAAGVKQLADDRTLLMAG
ECCCCCCHHHHHHHHHCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEC
VSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEECNAIIEQFIDYLRTGQEMPMEMAD
CCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
LNAVLGEVIAAESGYEREIETALYPGSIEVKMHPLSIKRAVANMVVNAARYGNGWIKLSS
HHHHHHHHHHCCCCCCHHHHHEECCCEEEEEECCHHHHHHHHHHHHHHHHCCCCEEEECC
GTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVRGDSARTISGTGLGLAIVQRIVDNHNGML
CCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHCCCCEE
ELGTSERGGLSIRAWLPVPVTRAQGTTKEG
EECCCCCCCEEEEEEECCCEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 6292200; 2997120; 9278503; 8132603; 2277041; 2824492; 1323560; 9817206; 10426948 [H]