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The map label for this gene is sorF [H]

Identifier: 187732005

GI number: 187732005

Start: 4229742

End: 4230149

Strand: Reverse

Name: sorF [H]

Synonym: SbBS512_E4522

Alternate gene names: 187732005

Gene position: 4230149-4229742 (Counterclockwise)

Preceding gene: 187731433

Following gene: 187734152

Centisome position: 91.64

GC content: 53.68

Gene sequence:

>408_bases
ATGGTCAATGCCATTTTTTGCGCCCACGTCAAATTAGCTTGCGCCATGCTGGAATCGGTTCAGATGGTGTACGGCGACGC
CAGGGTCGAGGCAGTTGAATTTGTGCCCGGCGAGAACGCCGGAGACATCGTAGCAAAGCTGGGAAGGTTAGTAAGCATTC
ACAATCAAGATGAGTGGCTGATTGCCGTGGATTTACAGTGCGGCAGCCCGTGGAATGCGGCAGCGATGCTTGCGATGCGC
AATCCTCGTCTGCGGGTAATCAGCGGCCTTTCTCTGCCGCTGGCGCTCGAACTGGTTGATAACCAGGACAGCATGAATGT
TGATGAAGTGTGTGAACACCTGACGCAAATCGCAAAGCAAACCTGCGTTGTCTGGAAACAACTGGCAACGGCAGAGGAGG
ATTTCTGA

Upstream 100 bases:

>100_bases
CGCTGATTTTGTTTGTTATCTGCTGTCTGAACGCGCCAGCTATATCACCGGAGTAACCACTAACATTGCGGGCGGCAAAA
CGCGCGGCTAAGGAGGCTTT

Downstream 100 bases:

>100_bases
TGAACATTACGCTCGCCCGCATTGATGACCGCCTGATCCACGGTCAGGTCACTACCGTCTGGTCGAAAGTGGCTAACGCC
CAGCGCATTATTATCTGCAA

Product: PTS system mannose/fructose/sorbose family IIA

Products: protein histidine; sugar phosphate

Alternate protein names: EIIA-Sor; EIII-F-Sor; PTS system sorbose-specific EIIA component [H]

Number of amino acids: Translated: 135; Mature: 135

Protein sequence:

>135_residues
MVNAIFCAHVKLACAMLESVQMVYGDARVEAVEFVPGENAGDIVAKLGRLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMR
NPRLRVISGLSLPLALELVDNQDSMNVDEVCEHLTQIAKQTCVVWKQLATAEEDF

Sequences:

>Translated_135_residues
MVNAIFCAHVKLACAMLESVQMVYGDARVEAVEFVPGENAGDIVAKLGRLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMR
NPRLRVISGLSLPLALELVDNQDSMNVDEVCEHLTQIAKQTCVVWKQLATAEEDF
>Mature_135_residues
MVNAIFCAHVKLACAMLESVQMVYGDARVEAVEFVPGENAGDIVAKLGRLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMR
NPRLRVISGLSLPLALELVDNQDSMNVDEVCEHLTQIAKQTCVVWKQLATAEEDF

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG2893

COG function: function code G; Phosphotransferase system, mannose/fructose-specific component IIA

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-4 domain [H]

Homologues:

None

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004701
- InterPro:   IPR013789 [H]

Pfam domain/function: PF03610 EIIA-man [H]

EC number: 2.7.1.69

Molecular weight: Translated: 14824; Mature: 14824

Theoretical pI: Translated: 4.33; Mature: 4.33

Prosite motif: PS51096 PTS_EIIA_TYPE_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.7 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
8.1 %Cys+Met (Translated Protein)
3.7 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
8.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNAIFCAHVKLACAMLESVQMVYGDARVEAVEFVPGENAGDIVAKLGRLVSIHNQDEWL
CCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHEEECCCCCEE
IAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQDSMNVDEVCEHLTQIAKQ
EEEEEECCCCCCHHHHEEECCCCEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHH
TCVVWKQLATAEEDF
HHHHHHHHHHCCCCC
>Mature Secondary Structure
MVNAIFCAHVKLACAMLESVQMVYGDARVEAVEFVPGENAGDIVAKLGRLVSIHNQDEWL
CCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHEEECCCCCEE
IAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQDSMNVDEVCEHLTQIAKQ
EEEEEECCCCCCHHHHEEECCCCEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHH
TCVVWKQLATAEEDF
HHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Transferring phosphorus-containing groups; Phosphotransferases with an alcohol group as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7947968 [H]