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The map label for this gene is insF1 [C]

Identifier: 187731432

GI number: 187731432

Start: 4078827

End: 4079645

Strand: Reverse

Name: insF1 [C]

Synonym: SbBS512_E4367

Alternate gene names: 187731432

Gene position: 4079645-4078827 (Counterclockwise)

Preceding gene: 187730138

Following gene: 187731097

Centisome position: 88.38

GC content: 51.16

Gene sequence:

>819_bases
ATGTGTCAGGTATTTGGTGTATCCAGGAGCGGTTATTACAACTGGGTACAGCATGAACCCTCAGACAGAAAACAAAGTGA
TGAGCGGCTAAAACTGGAGATTAAGGTGGCACATATCCGCACTCGCGAAACATATGGAACCCGGCGGCTCCAGACGGAGC
TGGCAGAGAATGGCATCATCGTTGGTCGTGACCGACTGGCACGTCTTCGTAAGGAGCTAAGGCTACGCTGTAAGCAGAAA
CGCAAGTTCAGAGCGACTACGAACCCGAACCACAATCTGCCAGTTGCGCCAAATCTGCTGAACCAGACGTTCGCTCCTAC
AGCACCAAATCAGGTCTGGGTGGCGGACCTGACGTATGTTGCCACACAGGAGGGATGGTTGTACCTCGCTGGCATCAAAG
ATGTTTATACGTGCGAAATTGTCGGCTACGCCATGGGAGAGCGCATGACAAAAGAGCTGACAGGTAAAGCCCTGTTTATG
GCGCTCAGGAGCCAGCGCCCACCTGCCGGGCTAATCCACCACTCTGATCGAGGTTCACAGTACTGCGCATACGATTACCG
GGTCATACAGGAGCAGTCTGGTCTGAAAACATCAATGTCGCGTAAAGGTCACTGTTACGACAACGCTCCGATGGAAAGCT
TCTGGGGAACGCTGAAAAATGAGAGCCTGAGCCACTATCGTTTTAATAACCAGGATGAAGCCATCTCAGTAATACGGGAA
TACATTGAGATTTTCTACAATCGTCAGCGTCGTCACTCTCGTCTGGGGAATATCTCCCCGGCAGCCTTCAGGGAAAAATA
TCATCAGATGGCTGCTTAA

Upstream 100 bases:

>100_bases
AGCGAGATATATTAAAAAAAGCAACAGCGTATTTTGCACAGGAGTCGCTGAAAAATACGCGTTAATCGAACAATGGCGAC
AACAATTTCCCATTGAAGCG

Downstream 100 bases:

>100_bases
AAAAAGAACAAATGGTAGTGTCCGCTATTGCCAGTACACCTCATTCTGATAAATGAAGAAAAACCGTATTCATCGCCATT
ACCACCACCTTACCTATATT

Product: ISSd1, transposase orfB

Products: NA

Alternate protein names: ISO-S3 31 kDa protein [H]

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MCQVFGVSRSGYYNWVQHEPSDRKQSDERLKLEIKVAHIRTRETYGTRRLQTELAENGIIVGRDRLARLRKELRLRCKQK
RKFRATTNPNHNLPVAPNLLNQTFAPTAPNQVWVADLTYVATQEGWLYLAGIKDVYTCEIVGYAMGERMTKELTGKALFM
ALRSQRPPAGLIHHSDRGSQYCAYDYRVIQEQSGLKTSMSRKGHCYDNAPMESFWGTLKNESLSHYRFNNQDEAISVIRE
YIEIFYNRQRRHSRLGNISPAAFREKYHQMAA

Sequences:

>Translated_272_residues
MCQVFGVSRSGYYNWVQHEPSDRKQSDERLKLEIKVAHIRTRETYGTRRLQTELAENGIIVGRDRLARLRKELRLRCKQK
RKFRATTNPNHNLPVAPNLLNQTFAPTAPNQVWVADLTYVATQEGWLYLAGIKDVYTCEIVGYAMGERMTKELTGKALFM
ALRSQRPPAGLIHHSDRGSQYCAYDYRVIQEQSGLKTSMSRKGHCYDNAPMESFWGTLKNESLSHYRFNNQDEAISVIRE
YIEIFYNRQRRHSRLGNISPAAFREKYHQMAA
>Mature_272_residues
MCQVFGVSRSGYYNWVQHEPSDRKQSDERLKLEIKVAHIRTRETYGTRRLQTELAENGIIVGRDRLARLRKELRLRCKQK
RKFRATTNPNHNLPVAPNLLNQTFAPTAPNQVWVADLTYVATQEGWLYLAGIKDVYTCEIVGYAMGERMTKELTGKALFM
ALRSQRPPAGLIHHSDRGSQYCAYDYRVIQEQSGLKTSMSRKGHCYDNAPMESFWGTLKNESLSHYRFNNQDEAISVIRE
YIEIFYNRQRRHSRLGNISPAAFREKYHQMAA

Specific function: Involved in the transposition of the insertion sequence is3 [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=243, Percent_Identity=31.2757201646091, Blast_Score=125, Evalue=5e-29,
Organism=Escherichia coli, GI1788405, Length=268, Percent_Identity=40.6716417910448, Blast_Score=203, Evalue=8e-54,
Organism=Escherichia coli, GI1787263, Length=268, Percent_Identity=40.6716417910448, Blast_Score=203, Evalue=8e-54,
Organism=Escherichia coli, GI1786753, Length=268, Percent_Identity=40.6716417910448, Blast_Score=203, Evalue=8e-54,
Organism=Escherichia coli, GI1786570, Length=268, Percent_Identity=40.6716417910448, Blast_Score=203, Evalue=8e-54,
Organism=Escherichia coli, GI1786490, Length=268, Percent_Identity=40.6716417910448, Blast_Score=203, Evalue=8e-54,
Organism=Escherichia coli, GI1789981, Length=264, Percent_Identity=32.1969696969697, Blast_Score=130, Evalue=7e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 31639; Mature: 31639

Theoretical pI: Translated: 10.04; Mature: 10.04

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCQVFGVSRSGYYNWVQHEPSDRKQSDERLKLEIKVAHIRTRETYGTRRLQTELAENGII
CCEEEECCCCCCCCHHCCCCCCCCCCCCEEEEEEEEEEEEEHHHHHHHHHHHHHHHCCEE
VGRDRLARLRKELRLRCKQKRKFRATTNPNHNLPVAPNLLNQTFAPTAPNQVWVADLTYV
EEHHHHHHHHHHHHHHHHHHHCEECCCCCCCCCCCCCHHHHCCCCCCCCCEEEEEEEEEE
ATQEGWLYLAGIKDVYTCEIVGYAMGERMTKELTGKALFMALRSQRPPAGLIHHSDRGSQ
EECCCEEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCC
YCAYDYRVIQEQSGLKTSMSRKGHCYDNAPMESFWGTLKNESLSHYRFNNQDEAISVIRE
EEEHHHHHHHHHCCCHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
YIEIFYNRQRRHSRLGNISPAAFREKYHQMAA
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MCQVFGVSRSGYYNWVQHEPSDRKQSDERLKLEIKVAHIRTRETYGTRRLQTELAENGII
CCEEEECCCCCCCCHHCCCCCCCCCCCCEEEEEEEEEEEEEHHHHHHHHHHHHHHHCCEE
VGRDRLARLRKELRLRCKQKRKFRATTNPNHNLPVAPNLLNQTFAPTAPNQVWVADLTYV
EEHHHHHHHHHHHHHHHHHHHCEECCCCCCCCCCCCCHHHHCCCCCCCCCEEEEEEEEEE
ATQEGWLYLAGIKDVYTCEIVGYAMGERMTKELTGKALFMALRSQRPPAGLIHHSDRGSQ
EECCCEEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCC
YCAYDYRVIQEQSGLKTSMSRKGHCYDNAPMESFWGTLKNESLSHYRFNNQDEAISVIRE
EEEHHHHHHHHHCCCHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
YIEIFYNRQRRHSRLGNISPAAFREKYHQMAA
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2824781 [H]