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The map label for this gene is pheP

Identifier: 187731101

GI number: 187731101

Start: 454126

End: 455502

Strand: Direct

Name: pheP

Synonym: SbBS512_E0470

Alternate gene names: 187731101

Gene position: 454126-455502 (Clockwise)

Preceding gene: 187730717

Following gene: 187731165

Centisome position: 9.84

GC content: 52.72

Gene sequence:

>1377_bases
GTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGCCGACGCTTCATCGCGGATTACATAACCGTCA
TATTCAACTGATTGCGCTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTC
CGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTCGGCGAGATGGTGGTCGAA
GAGCCGGTATCCGGTTCATTTGCCCACTTTGCCTATAAATACTGGGGACCGTTTGCGGGCTTCCTCTCTGGCTGGAACTA
CTGGGTAATGTTCGTGCTGGTGGGAATGGCAGAGCTGACCGCTGCGGGCATCTATATGCAGTACTGGTTCCCGGATGTTC
CAACGTGGATTTGGGCTGCCGCCTTCTTTATTATCATCAACGCCGTTAACCTGGTGAACGTGCGCTTATATGGCGAAACC
GAGTTCTGGTTTGCGTTGATTAAAGTGCTGGCAATCATCGGTATGATCGGCTTTGGCCTGTGGCTGCTGTTTTCTGGTCA
CGGCGGCGAGAAAGCCAGTATCGACAACCTCTGGTGCTACGGTGGTTTCTTCGCCACCGGCTGGAATGGGCTGATTTTGT
CGCTGGCGGTAATTATGTTCTCCTTCGGCGGTCTGGAGCTGATTGGGATTACTGCCGCTGAAGCGCGCGATCCGGAAAAA
AGCATTCCAAAAGCGGTAAATCAGGTGGTGTATCGCATCCTGCTGTTTTACATCGGTTCACTGGTGGTTTTACTGGCGCT
CTATCCGTGGGTGGAAGTGAAATCCAACAGTAGCCCGTTTGTGATGATTTTCCATAATCTCGACAGCAACGAGGTAGCTT
CTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTCAGTGTATAACAGCGGGGTTTACTCTAACAGCCGCATGCTGTTT
GGCCTTTCTGTGCAGGGTAATGCGCCGAAGTTTTTGACTCGCGTCAGCCATCGCGGCGTGCCGATTAACTCGCTGATGCT
TTCCGGAGCGATCACTTCGCTGGTGGTGTTAATCAACTATCTGCTGCCGCAAAAAGCGTTTGGTCTGCTGATGGCGCTGG
TGGTAGCAACGCTGCTGTTGAACTGGATTATGATCTGCCTGGCGCATCTGCGTTTTCGCGCGGCGATGCGACGTCAGGGA
CGTGAAACACAGTTTAAGGCGCTGCTTTATCCGTTCGGCAACTATCTTTGCATTGCCTTCCTCGGCATGATTTTGCTGCT
GATGTGCACGATGGATGATATGCGCTTGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTGTGGCATTTAAAA
CGCTGCGTCGGAAATAA

Upstream 100 bases:

>100_bases
AGCAGGATACCCCGTTTAACCGTGTGGATTGTGTCTTGCGACGATGGGCACTAAATGTTAAAAGGTGCCCCTCAACAAAA
AAGACACACAGGGGAAAGGC

Downstream 100 bases:

>100_bases
GGCATTCACGCTACATCCGACAAAACGATGTCCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAAT
GCACCCGCCAGAGAGCGAAT

Product: phenylalanine transporter

Products: Proton [Cytoplasm]; L-phenylalanine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLGIGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVE
EPVSGSFAHFAYKYWGPFAGFLSGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWCYGGFFATGWNGLILSLAVIMFSFGGLELIGITAAEARDPEK
SIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSNSSPFVMIFHNLDSNEVASALNFVILVASLSVYNSGVYSNSRMLF
GLSVQGNAPKFLTRVSHRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRFRAAMRRQG
RETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFVAFKTLRRK

Sequences:

>Translated_458_residues
MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLGIGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVE
EPVSGSFAHFAYKYWGPFAGFLSGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWCYGGFFATGWNGLILSLAVIMFSFGGLELIGITAAEARDPEK
SIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSNSSPFVMIFHNLDSNEVASALNFVILVASLSVYNSGVYSNSRMLF
GLSVQGNAPKFLTRVSHRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRFRAAMRRQG
RETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFVAFKTLRRK
>Mature_458_residues
MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLGIGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVE
EPVSGSFAHFAYKYWGPFAGFLSGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWCYGGFFATGWNGLILSLAVIMFSFGGLELIGITAAEARDPEK
SIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSNSSPFVMIFHNLDSNEVASALNFVILVASLSVYNSGVYSNSRMLF
GLSVQGNAPKFLTRVSHRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRFRAAMRRQG
RETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFVAFKTLRRK

Specific function: Permease that is involved in the transport across the cytoplasmic membrane of phenylalanine [H]

COG id: COG1113

COG function: function code E; Gamma-aminobutyrate permease and related permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid-polyamine-organocation (APC) superfamily. Amino acid transporter (AAT) (TC 2.A.3.1) family [H]

Homologues:

Organism=Homo sapiens, GI110347453, Length=290, Percent_Identity=27.5862068965517, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI181337167, Length=402, Percent_Identity=24.8756218905473, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI258645169, Length=441, Percent_Identity=21.9954648526077, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI115648063, Length=457, Percent_Identity=21.6630196936543, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI115648022, Length=457, Percent_Identity=21.6630196936543, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1786789, Length=458, Percent_Identity=99.1266375545851, Blast_Score=907, Evalue=0.0,
Organism=Escherichia coli, GI1786302, Length=434, Percent_Identity=64.0552995391705, Blast_Score=590, Evalue=1e-170,
Organism=Escherichia coli, GI48994972, Length=429, Percent_Identity=43.8228438228438, Blast_Score=365, Evalue=1e-102,
Organism=Escherichia coli, GI1786602, Length=452, Percent_Identity=44.6902654867257, Blast_Score=350, Evalue=1e-97,
Organism=Escherichia coli, GI1790653, Length=453, Percent_Identity=37.9690949227373, Blast_Score=335, Evalue=4e-93,
Organism=Escherichia coli, GI87081915, Length=425, Percent_Identity=36.2352941176471, Blast_Score=297, Evalue=7e-82,
Organism=Escherichia coli, GI1788480, Length=418, Percent_Identity=37.5598086124402, Blast_Score=270, Evalue=1e-73,
Organism=Escherichia coli, GI1789017, Length=431, Percent_Identity=36.6589327146172, Blast_Score=268, Evalue=5e-73,
Organism=Escherichia coli, GI87081708, Length=447, Percent_Identity=35.1230425055928, Blast_Score=266, Evalue=2e-72,
Organism=Escherichia coli, GI87081869, Length=175, Percent_Identity=28.5714285714286, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI87082023, Length=381, Percent_Identity=22.8346456692913, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI2367353, Length=370, Percent_Identity=21.3513513513514, Blast_Score=62, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17540018, Length=383, Percent_Identity=23.4986945169713, Blast_Score=72, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17531343, Length=391, Percent_Identity=21.9948849104859, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6320772, Length=448, Percent_Identity=35.9375, Blast_Score=246, Evalue=6e-66,
Organism=Saccharomyces cerevisiae, GI6324061, Length=443, Percent_Identity=34.9887133182844, Blast_Score=241, Evalue=3e-64,
Organism=Saccharomyces cerevisiae, GI6322892, Length=424, Percent_Identity=35.6132075471698, Blast_Score=212, Evalue=8e-56,
Organism=Saccharomyces cerevisiae, GI6324059, Length=450, Percent_Identity=34, Blast_Score=208, Evalue=1e-54,
Organism=Saccharomyces cerevisiae, GI6319824, Length=449, Percent_Identity=31.8485523385301, Blast_Score=208, Evalue=2e-54,
Organism=Saccharomyces cerevisiae, GI6321629, Length=416, Percent_Identity=31.9711538461538, Blast_Score=205, Evalue=1e-53,
Organism=Saccharomyces cerevisiae, GI6324990, Length=441, Percent_Identity=31.7460317460317, Blast_Score=204, Evalue=2e-53,
Organism=Saccharomyces cerevisiae, GI6324924, Length=448, Percent_Identity=31.25, Blast_Score=201, Evalue=3e-52,
Organism=Saccharomyces cerevisiae, GI6324553, Length=399, Percent_Identity=33.0827067669173, Blast_Score=199, Evalue=9e-52,
Organism=Saccharomyces cerevisiae, GI6321053, Length=439, Percent_Identity=31.6628701594533, Blast_Score=189, Evalue=5e-49,
Organism=Saccharomyces cerevisiae, GI6320717, Length=484, Percent_Identity=27.2727272727273, Blast_Score=189, Evalue=6e-49,
Organism=Saccharomyces cerevisiae, GI6319543, Length=436, Percent_Identity=31.651376146789, Blast_Score=185, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6322967, Length=395, Percent_Identity=31.1392405063291, Blast_Score=182, Evalue=7e-47,
Organism=Saccharomyces cerevisiae, GI6320251, Length=488, Percent_Identity=27.6639344262295, Blast_Score=178, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6319542, Length=480, Percent_Identity=26.4583333333333, Blast_Score=171, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6324981, Length=416, Percent_Identity=32.4519230769231, Blast_Score=170, Evalue=4e-43,
Organism=Saccharomyces cerevisiae, GI6319608, Length=409, Percent_Identity=29.8288508557457, Blast_Score=167, Evalue=4e-42,
Organism=Saccharomyces cerevisiae, GI6320364, Length=487, Percent_Identity=27.7207392197125, Blast_Score=134, Evalue=3e-32,
Organism=Drosophila melanogaster, GI221512776, Length=426, Percent_Identity=23.0046948356808, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24666159, Length=426, Percent_Identity=23.0046948356808, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI161077963, Length=416, Percent_Identity=22.3557692307692, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24667468, Length=427, Percent_Identity=22.7166276346604, Blast_Score=69, Evalue=7e-12,
Organism=Drosophila melanogaster, GI221331183, Length=351, Percent_Identity=23.6467236467236, Blast_Score=65, Evalue=8e-11,
Organism=Drosophila melanogaster, GI17647653, Length=351, Percent_Identity=23.6467236467236, Blast_Score=65, Evalue=8e-11,
Organism=Drosophila melanogaster, GI24664379, Length=351, Percent_Identity=23.6467236467236, Blast_Score=65, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004841
- InterPro:   IPR002293
- InterPro:   IPR004840 [H]

Pfam domain/function: PF00324 AA_permease [H]

EC number: NA

Molecular weight: Translated: 50604; Mature: 50604

Theoretical pI: Translated: 9.32; Mature: 9.32

Prosite motif: PS00218 AMINO_ACID_PERMEASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLGIGPAIQMAGPAVLLGY
CCCCCCCCCCCCCCCCCHHHHCCCCCCEEEEEECCHHHHHHHHHCCHHHHHCCCHHHHHH
GVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFLSGWNYWVMFVLVGMAELT
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
AAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGETEFWFALIKVLAIIGMIGFGL
HHHHHHEECCCCCHHHHHHHHHHHHHHHHHEEEEEEECCHHHHHHHHHHHHHHHHHHHHH
WLLFSGHGGEKASIDNLWCYGGFFATGWNGLILSLAVIMFSFGGLELIGITAAEARDPEK
HHHCCCCCCCCCCCCCEEEECCCHHCCHHHHHHHHHHHHHHCCCEEEEEEEHHCCCCCHH
SIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSNSSPFVMIFHNLDSNEVASALNFVI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCEEEEEECCCCHHHHHHHHHHH
LVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTRVSHRGVPINSLMLSGAITSLVVLINY
HHHHHHHHHCCCCCCCEEEEEEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
LLPQKAFGLLMALVVATLLLNWIMICLAHLRFRAAMRRQGRETQFKALLYPFGNYLCIAF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
LGMILLLMCTMDDMRLSAILLPVWIVFLFVAFKTLRRK
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLGIGPAIQMAGPAVLLGY
CCCCCCCCCCCCCCCCCHHHHCCCCCCEEEEEECCHHHHHHHHHCCHHHHHCCCHHHHHH
GVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFLSGWNYWVMFVLVGMAELT
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
AAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGETEFWFALIKVLAIIGMIGFGL
HHHHHHEECCCCCHHHHHHHHHHHHHHHHHEEEEEEECCHHHHHHHHHHHHHHHHHHHHH
WLLFSGHGGEKASIDNLWCYGGFFATGWNGLILSLAVIMFSFGGLELIGITAAEARDPEK
HHHCCCCCCCCCCCCCEEEECCCHHCCHHHHHHHHHHHHHHCCCEEEEEEEHHCCCCCHH
SIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSNSSPFVMIFHNLDSNEVASALNFVI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCEEEEEECCCCHHHHHHHHHHH
LVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTRVSHRGVPINSLMLSGAITSLVVLINY
HHHHHHHHHCCCCCCCEEEEEEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
LLPQKAFGLLMALVVATLLLNWIMICLAHLRFRAAMRRQGRETQFKALLYPFGNYLCIAF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
LGMILLLMCTMDDMRLSAILLPVWIVFLFVAFKTLRRK
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; L-phenylalanine [Periplasm] [C]

Specific reaction: Proton [Periplasm] + L-phenylalanine [Periplasm] = Proton [Cytoplasm] + L-phenylalanine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1711024; 8905232; 9278503; 8626334 [H]