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The map label for this gene is mrcB

Identifier: 187730660

GI number: 187730660

Start: 151800

End: 154322

Strand: Direct

Name: mrcB

Synonym: SbBS512_E0140

Alternate gene names: 187730660

Gene position: 151800-154322 (Clockwise)

Preceding gene: 187730081

Following gene: 187731816

Centisome position: 3.29

GC content: 55.21

Gene sequence:

>2523_bases
ATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTCAAACAAAAGGTAAGCCGTCGTCG
TTACGAAGATGACGATGATTACGACGATTATGATGACTATGAGGATGAAGAACCGATGCCGCGCAAAGGTAAGGGCAAAG
GCAAAGGGCGTAAGCCTCGTGGCAAACGCGGCTGGCTATGGCTACTGCTAAAACTGGCTATCGTTTTTGCCGTGCTGATC
GCCATTTACGGCGTTTATCTCGATCAAAAAATTCGTAGCCGTATTGATGGCAAGGTCTGGCAACTGCCTGCGGCAGTTTA
TGGCCGAATGGTCAATCTTGAGCCAGATATGACCATCAGCAAGAACGAGATGGTGAAGCTGCTGGAGGCGACTCAGTATC
GTCAGGTGTCGAAAATGACCCGTCCTGGCGAATTTACCGTGCAGGCCAACAGCATTGAGATGATCCGCCGTCCGTTTGAT
TTCCCGGACAGTAAAGAAGGGCAGGTGCGTGCGCGTCTGACCTTTGATGGTGATCATCTGGCGACCATCGTCAATATGGA
GAACAACCGTCAGTTCGGCTTTTTCCGTCTTGATCCGCGTCTGATCACCATGATCTCCTCGCCAAACGGTGAGCAACGTC
TGTTTGTGCCGCGCAGTGGTTTCCCTGATTTGCTGGTGGATACTTTGCTGGCGACAGAAGACCGCCATTTCTACGAGCAT
GATGGGATCAGCCTCTACTCAATTGGGCGTGCGGTGCTGGCAAACCTGACCGCAGGTCGCACGGTGCAGGGTGCGAGTAC
GCTGACGCAACAGCTGGTGAAAAACCTGTTCCTCTCCAGCGAGCGCTCTTACTGGCGTAAAGCGAACGAAGCCTACATGG
CGCTGATCATGGACGCGCGTTACAGCAAAGACCGTATTCTTGAGCTGTATATGAACGAGGTGTATCTCGGTCAGAGCGGC
GACAACGAAATCCGCGGCTTCCCGCTGGCAAGCTTGTATTACTTTGGTCGCCCGGTAGAAGAGCTAAGCCTCGACCAGCA
GGCGCTGTTAGTCGGTATGGTGAAAGGGGCGTCCATCTACAACCCGTGGCGTAACCCGAAACTGGCGCTGGAGCGACGTA
ATCTGGTGCTGCGTCTGCTGCAACAGCAACAGATTATTGATCAAGAACTCTATGACATGTTGAGTGCCCGTCCGCTGGGG
GTTCAGCCGCGCGGTGGGGTGATCTCTCCTCAGCCAGCCTTTATGCAACTGGTGCGTCAGGAGCTGCAGGCAAAACTGGG
CGATAAGGTAAAAGATCTCTCCGGCGTGAAGATCTTCACTACCTTTGACTCGGTGGCCCAGGACGCGGCAGAAAAAGCCG
TCGTGGAAGGCATTCCGGCACTGAAGAAACAGCGTAAGTTGAGCGATCTTGAAACTGCGATTGTGATCGTCGACCGCTTT
AGTGGTGAAGTTCGTGCGATGGTGGGAGGTTCTGAGCCGCAGTTTGCGGGCTACAACCGTGCGATGCAGGCGCGTCGTTC
GATTGGTTCCCTTGCAAAACCAGCGACTTATCTGACGGCTTTAAGCCAGCCGAAAATCTATCGCCTGAACACGTGGATTG
CCGATGCGCCAATCGCGCTGCGTCAGCCGAATGGCCAGGTCTGGTCACCGCAGAACGATGACCGTCGTTATAGCGAAAGC
GGCAGAGTGATGCTGGTGGATGCGTTGACCCGTTCGATGAACGTGCCGACGGTAAATCTGGGGATGGCGCTGGGGCTGCC
TGCAGTTACGGATACCTGGATCAAACTGGGCGTACCGAAAGATCAGCTGCATCCGGTTCCGGCGATGCTGCTGGGGGCGT
TGAACTTAACGCCAATCGAAGTGGCGCAGGCGTTCCAGACCATCGCCAGCGGTGGTAACCGTGCACCGCTTTCTGCACTG
CGTTCGGTAATCGCGGAAGATGGCAAGGTGCTGTATCAGAGCTTCCCGCAGGCAGAACGCGCTGTTCCGGCGCAGGCGGC
GTATCTGACACTATGGACCATGCAGCAGGTGGTACAACGTGGTACGGGTCGTCAGCTTGGGGCGAAATACCCGAACCTGC
ATCTGGCAGGGAAAACAGGGACTACCAACAATAACGTAGATACTTGGTTTGCAGGCATTGACGGCAGCACGGTGACCATC
ACCTGGGTAGGCCGTGATAACAACCAGCCGACCAAACTGTATGGAGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCT
GGCTAACCAGACGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCGTGGACTACGACGGCAACT
TTGTTTGCAGCGGTGGCATGCGTGTCTTGCCGGTCTGGACCAGCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAG
CAGCAGCCGTCAGGCAATCCGTTTGATCAGTCTTCTCAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGA
CGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAA

Upstream 100 bases:

>100_bases
GGGACCGATCGAACACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCT
GAATATTGCGGAGAAAAAGC

Downstream 100 bases:

>100_bases
CATCTAAGCGTGAAATACCGGATGGCGAGTTGCCATCCGGTAAAATAACATCCCATCTAAGATATTAACCCTTTCTTTTC
ATCTGGTTGTTTATTAACCC

Product: penicillin-binding protein 1b

Products: NA

Alternate protein names: PBP-1b; PBP1b; Murein polymerase; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Peptidoglycan glycosyltransferase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]

Number of amino acids: Translated: 840; Mature: 839

Protein sequence:

>840_residues
MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEEPMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLI
AIYGVYLDQKIRSRIDGKVWQLPAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPRSGFPDLLVDTLLATEDRHFYEH
DGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNLFLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSG
DNEIRGFPLASLYYFGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYDMLSARPLG
VQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAAEKAVVEGIPALKKQRKLSDLETAIVIVDRF
SGEVRAMVGGSEPQFAGYNRAMQARRSIGSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSES
GRVMLVDALTRSMNVPTVNLGMALGLPAVTDTWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQAFQTIASGGNRAPLSAL
RSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGTGRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTI
TWVGRDNNQPTKLYGASGAMSIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSEMQ
QQPSGNPFDQSSQPQQQPAQQEQKDSDGVAGWIKDMFGSN

Sequences:

>Translated_840_residues
MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEEPMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLI
AIYGVYLDQKIRSRIDGKVWQLPAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPRSGFPDLLVDTLLATEDRHFYEH
DGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNLFLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSG
DNEIRGFPLASLYYFGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYDMLSARPLG
VQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAAEKAVVEGIPALKKQRKLSDLETAIVIVDRF
SGEVRAMVGGSEPQFAGYNRAMQARRSIGSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSES
GRVMLVDALTRSMNVPTVNLGMALGLPAVTDTWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQAFQTIASGGNRAPLSAL
RSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGTGRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTI
TWVGRDNNQPTKLYGASGAMSIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSEMQ
QQPSGNPFDQSSQPQQQPAQQEQKDSDGVAGWIKDMFGSN
>Mature_839_residues
AGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEEPMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIA
IYGVYLDQKIRSRIDGKVWQLPAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFDF
PDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPRSGFPDLLVDTLLATEDRHFYEHD
GISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNLFLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGD
NEIRGFPLASLYYFGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYDMLSARPLGV
QPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAAEKAVVEGIPALKKQRKLSDLETAIVIVDRFS
GEVRAMVGGSEPQFAGYNRAMQARRSIGSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESG
RVMLVDALTRSMNVPTVNLGMALGLPAVTDTWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQAFQTIASGGNRAPLSALR
SVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGTGRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTIT
WVGRDNNQPTKLYGASGAMSIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSEMQQ
QPSGNPFDQSSQPQQQPAQQEQKDSDGVAGWIKDMFGSN

Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=844, Percent_Identity=98.696682464455, Blast_Score=1557, Evalue=0.0,
Organism=Escherichia coli, GI87082258, Length=252, Percent_Identity=34.9206349206349, Blast_Score=153, Evalue=5e-38,
Organism=Escherichia coli, GI1788867, Length=515, Percent_Identity=26.6019417475728, Blast_Score=122, Evalue=8e-29,
Organism=Escherichia coli, GI1789601, Length=127, Percent_Identity=37.007874015748, Blast_Score=78, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011813
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: =2.4.1.129 [H]

Molecular weight: Translated: 93826; Mature: 93695

Theoretical pI: Translated: 9.46; Mature: 9.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEEPMPRKGKGKGKGRKPR
CCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQLPAAVYGRMVNLEPDMTIS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECHHHHCEEEECCCCCEEC
KNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFDFPDSKEGQVRARLTFDGDHL
HHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHCCCCCCCCCCCCEEEEEEECCCEE
ATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPRSGFPDLLVDTLLATEDRHFYEH
EEEEEECCCCEEEEEEECCEEEEEEECCCCCCEEEECCCCCHHHHHHHHHHCCCCCHHHC
DGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNLFLSSERSYWRKANEAYMALIMDAR
CCEEHHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHHHCCCHHHHHHHCHHHEEEEECCC
YSKDRILELYMNEVYLGQSGDNEIRGFPLASLYYFGRPVEELSLDQQALLVGMVKGASIY
CCHHHHHHHHHHHEEECCCCCCCCCCCCHHHHHHHCCCHHHHCCCHHHHHHHHHCCCCCC
NPWRNPKLALERRNLVLRLLQQQQIIDQELYDMLSARPLGVQPRGGVISPQPAFMQLVRQ
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHH
ELQAKLGDKVKDLSGVKIFTTFDSVAQDAAEKAVVEGIPALKKQRKLSDLETAIVIVDRF
HHHHHHCCHHHHCCCCEEEEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHEEEEEEECC
SGEVRAMVGGSEPQFAGYNRAMQARRSIGSLAKPATYLTALSQPKIYRLNTWIADAPIAL
CCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEEECEEECCCEEE
RQPNGQVWSPQNDDRRYSESGRVMLVDALTRSMNVPTVNLGMALGLPAVTDTWIKLGVPK
ECCCCCCCCCCCCCCHHCCCCCEEEEEHHHHCCCCCEEECHHHHCCCHHHHHHEEECCCH
DQLHPVPAMLLGALNLTPIEVAQAFQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAER
HHCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHCCHHHH
AVPAQAAYLTLWTMQQVVQRGTGRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTI
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCCCCEEEEECCCCEEEE
TWVGRDNNQPTKLYGASGAMSIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGM
EEEECCCCCCCEEECCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCCCCEEECCCC
RVLPVWTSDPQSLCQQSEMQQQPSGNPFDQSSQPQQQPAQQEQKDSDGVAGWIKDMFGSN
EEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCHHCHHHHHHCCCCCHHHHHHHHCCCC
>Mature Secondary Structure 
AGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEEPMPRKGKGKGKGRKPR
CCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQLPAAVYGRMVNLEPDMTIS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECHHHHCEEEECCCCCEEC
KNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFDFPDSKEGQVRARLTFDGDHL
HHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHCCCCCCCCCCCCEEEEEEECCCEE
ATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPRSGFPDLLVDTLLATEDRHFYEH
EEEEEECCCCEEEEEEECCEEEEEEECCCCCCEEEECCCCCHHHHHHHHHHCCCCCHHHC
DGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNLFLSSERSYWRKANEAYMALIMDAR
CCEEHHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHHHCCCHHHHHHHCHHHEEEEECCC
YSKDRILELYMNEVYLGQSGDNEIRGFPLASLYYFGRPVEELSLDQQALLVGMVKGASIY
CCHHHHHHHHHHHEEECCCCCCCCCCCCHHHHHHHCCCHHHHCCCHHHHHHHHHCCCCCC
NPWRNPKLALERRNLVLRLLQQQQIIDQELYDMLSARPLGVQPRGGVISPQPAFMQLVRQ
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHH
ELQAKLGDKVKDLSGVKIFTTFDSVAQDAAEKAVVEGIPALKKQRKLSDLETAIVIVDRF
HHHHHHCCHHHHCCCCEEEEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHEEEEEEECC
SGEVRAMVGGSEPQFAGYNRAMQARRSIGSLAKPATYLTALSQPKIYRLNTWIADAPIAL
CCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEEECEEECCCEEE
RQPNGQVWSPQNDDRRYSESGRVMLVDALTRSMNVPTVNLGMALGLPAVTDTWIKLGVPK
ECCCCCCCCCCCCCCHHCCCCCEEEEEHHHHCCCCCEEECHHHHCCCHHHHHHEEECCCH
DQLHPVPAMLLGALNLTPIEVAQAFQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAER
HHCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHCCHHHH
AVPAQAAYLTLWTMQQVVQRGTGRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTI
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCCCCEEEEECCCCEEEE
TWVGRDNNQPTKLYGASGAMSIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGM
EEEECCCCCCCEEECCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCCCCEEECCCC
RVLPVWTSDPQSLCQQSEMQQQPSGNPFDQSSQPQQQPAQQEQKDSDGVAGWIKDMFGSN
EEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCHHCHHHHHHCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3882429; 8202364; 9278503; 3920658; 8645198; 9244263; 3330753; 1885547; 10037771; 10564478; 9841666 [H]