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The map label for this gene is hyfF [H]

Identifier: 187730473

GI number: 187730473

Start: 2655177

End: 2656730

Strand: Direct

Name: hyfF [H]

Synonym: SbBS512_E2858

Alternate gene names: 187730473

Gene position: 2655177-2656730 (Clockwise)

Preceding gene: 187731081

Following gene: 187733753

Centisome position: 57.52

GC content: 54.12

Gene sequence:

>1554_bases
ATGAGTTATTCTGTGATGTTCGCTTTACTCCTGCTCACGCCGCTGCTTTTTTCGCTGCTCTGTTTTGCCTGCCGGAAACG
GGGGCTCTCTGCGACTTGTACGGTGACTGTATTACATAGCTTAGGGATCACACTGCTGCTGATTCTGGCACTCTGGGTGG
TCCAAACTGCCGCTGATGCAGGAGAAATATTCGCTGCGGGACTGTGGCTTCATATTGATGGTCTGGGCGGTTTGTTCCTC
GCCATTCTTGGTGTGATTGGCTACATGCGTCATGAAGTGGCACACGGCGAGCTTTCACCCGTTACACTGTGCGATTACTA
CGGTTTCTTCCATCTGTTTTTGTTCACCATGCTGCTGGTTGTTACCAGCAATAACCTGATTGTGATGTGGGCGGCGATCG
AAGCCACCACCTTAAGCTCGGCGTTTCTGGTAGGCATTTACGGTCAGCGTTCATCGCTGGAATCAGCATGGAAGTACATC
ATTATTTGTACTGTTGGTGTCGCTTTTGGTCTGTTCGGTACCGTGCTGGTATACGCCAACGCCGCCAGCGTTATGCCGCA
GGCAGAAATGGCGATATTCTGGAGCGAGATTCTTAAGCAATCGTCCTTGCTTGACCCAACATTAATGCTGTTGGCCTTTG
TGTTTGTGCTAATTGGCTTTGGTACCAAAACCGGGCTATTTCCCATGCACGCCTGGCTGCCGGATGCTCACAGTGAAGCG
CCGAGTCCGGTCAGCGCCCTGCTCTCCGCCGTATTGCTGAACTGCGCGCTGTTGGTGCTGATTCGCTATTACATCATTAT
TTGCCAAGCCATCGGCAGCGATTTCCCCAACCGGTTGTTGCTCATCTTCGGCATGTTGTCGGTTGCCGTGGCGGCATTTT
TCATTCTGGTACAGCGGGACATTAAGCGTCTGCTGGCGTACTCCAGCGTGGAGAACATGGGGCTGGTCGCGGTGGCGCTA
GGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACACCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTG
CGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTA
CCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACC
GTTACCGCAGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGCTGTTAACGCTGGTGCTGGCGGGCCT
GGTACGGATGGCTGCGCGGGTGTTAATGGCGAAACCGCCGCAGGCCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCT
CGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCATCAGGATCCTGGCGGGC
GCTTCCACTATAGTCCTCTCAGGGACGCACGACCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCTTCAGGCAC
CGCATCTGTTTCGGAGAAACACAGTGAACGTTAA

Upstream 100 bases:

>100_bases
ATCTTCGCCGTCATTGTGATGGTGTTACTGGCAAGAAAAATATGGCGTACCCACGGCACGCTGGACGTGAACAACTTGAC
CGCGCTGAAGGGATAATGAG

Downstream 100 bases:

>100_bases
TTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGAC
AAACGCCTGAACAGGTCACC

Product: hydrogenase 4 subunit F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 517; Mature: 516

Protein sequence:

>517_residues
MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGITLLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFL
AILGVIGYMRHEVAHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRSSLESAWKYI
IICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEILKQSSLLDPTLMLLAFVFVLIGFGTKTGLFPMHAWLPDAHSEA
PSPVSALLSAVLLNCALLVLIRYYIIICQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVAL
GIGGPLGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGALALAGMPPFNIFLSEFMT
VTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQAVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAG
ASTIVLSGTHDLPAQRSTWHDFLPSGTASVSEKHSER

Sequences:

>Translated_517_residues
MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGITLLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFL
AILGVIGYMRHEVAHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRSSLESAWKYI
IICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEILKQSSLLDPTLMLLAFVFVLIGFGTKTGLFPMHAWLPDAHSEA
PSPVSALLSAVLLNCALLVLIRYYIIICQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVAL
GIGGPLGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGALALAGMPPFNIFLSEFMT
VTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQAVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAG
ASTIVLSGTHDLPAQRSTWHDFLPSGTASVSEKHSER
>Mature_516_residues
SYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGITLLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLA
ILGVIGYMRHEVAHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRSSLESAWKYII
ICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEILKQSSLLDPTLMLLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAP
SPVSALLSAVLLNCALLVLIRYYIIICQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALG
IGGPLGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGALALAGMPPFNIFLSEFMTV
TAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQAVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGA
STIVLSGTHDLPAQRSTWHDFLPSGTASVSEKHSER

Specific function: Unknown

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=379, Percent_Identity=28.4960422163588, Blast_Score=109, Evalue=8e-24,
Organism=Homo sapiens, GI251831116, Length=185, Percent_Identity=31.3513513513514, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1788831, Length=526, Percent_Identity=96.7680608365019, Blast_Score=952, Evalue=0.0,
Organism=Escherichia coli, GI1788827, Length=326, Percent_Identity=30.9815950920245, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1788613, Length=423, Percent_Identity=27.8959810874704, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1788829, Length=371, Percent_Identity=30.4582210242588, Blast_Score=112, Evalue=7e-26,
Organism=Escherichia coli, GI1788614, Length=431, Percent_Identity=27.1461716937355, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI2367154, Length=194, Percent_Identity=36.0824742268041, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI145693160, Length=205, Percent_Identity=32.6829268292683, Blast_Score=84, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 55596; Mature: 55464

Theoretical pI: Translated: 8.17; Mature: 8.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGITLLLILALWVVQTAADA
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
GEIFAAGLWLHIDGLGGLFLAILGVIGYMRHEVAHGELSPVTLCDYYGFFHLFLFTMLLV
HHHHHHHHEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
VTSNNLIVMWAAIEATTLSSAFLVGIYGQRSSLESAWKYIIICTVGVAFGLFGTVLVYAN
HHCCCEEEEEEEHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AASVMPQAEMAIFWSEILKQSSLLDPTLMLLAFVFVLIGFGTKTGLFPMHAWLPDAHSEA
HHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCC
PSPVSALLSAVLLNCALLVLIRYYIIICQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
IKRLLAYSSVENMGLVAVALGIGGPLGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRD
HHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCH
LNVVCGMLKIMPFTAVLFGGGALALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLL
HHHHHHHHHHHHHHHHHHCCCHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLVLAGLVRMAARVLMAKPPQAVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAG
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHC
ASTIVLSGTHDLPAQRSTWHDFLPSGTASVSEKHSER
CCEEEEECCCCCCCCCCHHHHHCCCCCCHHHHHCCCC
>Mature Secondary Structure 
SYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGITLLLILALWVVQTAADA
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
GEIFAAGLWLHIDGLGGLFLAILGVIGYMRHEVAHGELSPVTLCDYYGFFHLFLFTMLLV
HHHHHHHHEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
VTSNNLIVMWAAIEATTLSSAFLVGIYGQRSSLESAWKYIIICTVGVAFGLFGTVLVYAN
HHCCCEEEEEEEHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AASVMPQAEMAIFWSEILKQSSLLDPTLMLLAFVFVLIGFGTKTGLFPMHAWLPDAHSEA
HHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCC
PSPVSALLSAVLLNCALLVLIRYYIIICQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
IKRLLAYSSVENMGLVAVALGIGGPLGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRD
HHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCH
LNVVCGMLKIMPFTAVLFGGGALALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLL
HHHHHHHHHHHHHHHHHHCCCHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLVLAGLVRMAARVLMAKPPQAVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAG
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHC
ASTIVLSGTHDLPAQRSTWHDFLPSGTASVSEKHSER
CCEEEEECCCCCCCCCCHHHHHCCCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]