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The map label for this gene is yneJ [H]

Identifier: 187730386

GI number: 187730386

Start: 1529187

End: 1530068

Strand: Reverse

Name: yneJ [H]

Synonym: SbBS512_E1680

Alternate gene names: 187730386

Gene position: 1530068-1529187 (Counterclockwise)

Preceding gene: 187732260

Following gene: 187732169

Centisome position: 33.15

GC content: 53.74

Gene sequence:

>882_bases
ATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCAGCAGCAATAGTGCTTCG
CGTGCCGTCGAATCTCACTACCCGTCTACGCCAGCTGGAAACAGAACTGGGGGTAGATCTGTTTATTCGCGAGAATCAGC
GTTTACGTCTCTCTCCTGCCGGGCATAACTTTTTACGCTATAGCCAACAAATTCTCGCGTTAGTGGATGAAGCGCGGAGC
GTTGTCGCTGGCGATGAGCCGCAAGGTTTATTTTCTCTTGGTTCGCTGGAAAGTACCGCAGCAGTGCGCATTCCGGCCAC
GCTGGCGGAATTCAACCATCGTTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCACTATGCTGGAAGGAG
TGCTGGAGGGAAAACTGAATGCGGCATTTATTGATGGGCCCATTAACCATACTGCCATTGACGGGATACCGGTATACCGC
GAGGAGCTGATGATCGTCACGCCACAAGGTCATGCGCCAGTAATCCGCGCCAGTCAGGTTAACGGCAGTAACATTTATGC
CTTCCGCGCCAATTGTTCGTATCGTCGCCACTTCGAGAGCTGGTTTCATGCTGACGGTGCCGCTCCGGGAACTATTCACG
AGATGGAGTCGTATCACGGGATGTTGGCCTGTGTGGTCGCAGGAGCAGGCATTGCGCTTATTCCACGCTCTATGCTGGAA
AGTATGCCGGGGCATCACCAGGTTGAAGCGTGGCCGTTAGCTGAGCAATGGCGTTGGTTAACAACCTGGCTGGTCTGGCG
TCGTGGTGCGAAAACGCGTCCGCTTGAGGCATTTATTCAACTACTGGATGTGCCTGACTCGGCAAAACAGGGTTACCAAT
GA

Upstream 100 bases:

>100_bases
CGGGGTATCTCCTTTATGAGTCATGGTATGAAGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTT
ATTCACGAATCGAGAATATT

Downstream 100 bases:

>100_bases
GCTATTTTTGATAGTTCTTGCAGCTCAAATGCCCTATATAGTGATGTTCCGATGACTTATGACTATATGGGGCAAATATG
GTTACGCCAGTAAGCATCAG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MDLTQLEMFNAVAEAGSITQAAAIVLRVPSNLTTRLRQLETELGVDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARS
VVAGDEPQGLFSLGSLESTAAVRIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESYHGMLACVVAGAGIALIPRSMLE
SMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLEAFIQLLDVPDSAKQGYQ

Sequences:

>Translated_293_residues
MDLTQLEMFNAVAEAGSITQAAAIVLRVPSNLTTRLRQLETELGVDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARS
VVAGDEPQGLFSLGSLESTAAVRIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESYHGMLACVVAGAGIALIPRSMLE
SMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLEAFIQLLDVPDSAKQGYQ
>Mature_293_residues
MDLTQLEMFNAVAEAGSITQAAAIVLRVPSNLTTRLRQLETELGVDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARS
VVAGDEPQGLFSLGSLESTAAVRIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESYHGMLACVVAGAGIALIPRSMLE
SMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLEAFIQLLDVPDSAKQGYQ

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787806, Length=293, Percent_Identity=97.2696245733788, Blast_Score=589, Evalue=1e-170,
Organism=Escherichia coli, GI1788887, Length=292, Percent_Identity=25.6849315068493, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI87082132, Length=287, Percent_Identity=22.9965156794425, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1788481, Length=179, Percent_Identity=31.2849162011173, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790208, Length=256, Percent_Identity=25, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1790262, Length=290, Percent_Identity=23.448275862069, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI157672245, Length=284, Percent_Identity=23.2394366197183, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI145693105, Length=288, Percent_Identity=21.5277777777778, Blast_Score=71, Evalue=6e-14,
Organism=Escherichia coli, GI1787879, Length=285, Percent_Identity=23.859649122807, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1787601, Length=286, Percent_Identity=21.6783216783217, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788297, Length=258, Percent_Identity=24.031007751938, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32383; Mature: 32383

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLTQLEMFNAVAEAGSITQAAAIVLRVPSNLTTRLRQLETELGVDLFIRENQRLRLSPA
CCCHHHHHHHHHHHCCCHHHHEEEEEECCCHHHHHHHHHHHHCCEEEEEECCCEEEECCC
GHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAVRIPATLAEFNHRYPKIQF
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCEEEECCHHHHHHCCCCCEEEE
SLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYREELMIVTPQGHAPVIRASQV
EECCCCCCHHHHHHHHCCCCEEEECCCCCCCEECCCCEECCCEEEECCCCCCCEEEEEEC
NGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESYHGMLACVVAGAGIALIPRSMLE
CCCEEEEEECCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
SMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLEAFIQLLDVPDSAKQGYQ
HCCCCCCEEECCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHCCCC
>Mature Secondary Structure
MDLTQLEMFNAVAEAGSITQAAAIVLRVPSNLTTRLRQLETELGVDLFIRENQRLRLSPA
CCCHHHHHHHHHHHCCCHHHHEEEEEECCCHHHHHHHHHHHHCCEEEEEECCCEEEECCC
GHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAVRIPATLAEFNHRYPKIQF
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCEEEECCHHHHHHCCCCCEEEE
SLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYREELMIVTPQGHAPVIRASQV
EECCCCCCHHHHHHHHCCCCEEEECCCCCCCEECCCCEECCCEEEECCCCCCCEEEEEEC
NGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESYHGMLACVVAGAGIALIPRSMLE
CCCEEEEEECCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
SMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLEAFIQLLDVPDSAKQGYQ
HCCCCCCEEECCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]