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The map label for this gene is insG [H]

Identifier: 187730252

GI number: 187730252

Start: 1539077

End: 1540705

Strand: Direct

Name: insG [H]

Synonym: SbBS512_E1691

Alternate gene names: 187730252

Gene position: 1539077-1540705 (Clockwise)

Preceding gene: 187733577

Following gene: 187732175

Centisome position: 33.34

GC content: 54.76

Gene sequence:

>1629_bases
TTGTTTCCGGATTTTTTTATGCACATTGGACAGGCTCTTGATCTGGTATCCCGTTACGATTCTCTGCGTAACCCACTGAC
TTCTCTGGGGGATTACCTCGACCCCGAACTCATCTCTCGTTGCCTTGCCGAATCAGGTACTGTAACGCTACGCAAGCGCC
GCCTTCCCCTCGAAATGATGGTCTGGTGTATTGTTGGCATGGCGCTTGAGCGTAAAGAACCTCTTCACCAGATTGTGAAT
CGCCTGGACATCATGCTGCCGGGCAATCGCCCCTTCGTTGCCCCCAGTGCCGTTATTCAGGCCCGCCAGCGCCTGGGAAG
TGAGGCTGTCCGCCGCGTGTTCACGAAAACAGCGCAGCTCTGGCATAACGCCACGCCGCATCCGCACTGGTGCGGCCTGA
CCCTGCTGGCCATCGATGGTGTGTTCTGGCGCACACCGGATACACCAGAGAACGATGCAGCCTTCCCCCGCCAGACACAT
GCCGGGAACCCGGCGCTCCACCCGCAGGTCAAAATGGTCTGCCAGATGGAACTGACCAGCCATCTGCTGACGGCTGCAGC
CTTCGGCACGATGAAGAACAGCGAAAATGAGCTTGCTGAGCAACTTATAGAACAAACCGGCGATAACACTCTGACGTTAA
TGGATAAAGGTTATTACTCACTGGGACTGTTAAATGCCTGGAGCCTGGCGGGAGAACACCGCCACTGGATGATACCTCTC
AGAAAGGGAGCGCAATATGAAGAGCTCAGAAAACTGGGTAAAGGCGATCATCTGGTGAAGCTGAAAACCAGCCCGCAGGC
ACGAAAAAAGTGGCCGGGACTGGGAAATGAAGTGACAGCCCGCCTGCTGACCGTGACGCGCAAAGGAAAAGTCTGCCATC
TGCTGACGTCGATGACGGACGCCATGCGCTTCCCCGGAGGAGAAATGGCGGATCTGTACAGTCATCGCTGGGAAATTGAA
CTGGGATACAGGGAGATAAAACAGACGATGCAACTGAGCAGGCTGACGCTGAGAAGTAAAAAGCCGGAGCTTGTGGAGCA
AGAGCTGTGGGGTGTCTTACTGGCTTATAATCTGGTGAGATATCAGATGATTAAAATGGCGGAAAGTGGCGCAGTAGACT
GTGACGTTTTTTTTGACGACAGGGACCAGGCAGTCCCCTACACAGCCACCGCTGATGATGTCGCTCCGACGGGTCAGCAA
ATCTGGCAGGAACTGCAAAGCGGCAAATGGGGTGAGATAGCCCCATTCACTGTGACACCAGAAATGCTGGAAGCGGCCAG
AGAGGCCAGACGTCAGGAAATTGAAGCATGGCGCGCAGAACAGGAGGCGAAGCCGTTCACGTTTGAATGGAACGGTCGTA
TCTGGAATGCTGGTCCCGACTCACTGGGCCGCCTGTCCCCGGTAGTCATGCTGGCAAAATCTGTCACAGCACAAACACAT
ATGGCGTGGAGCGATGCCGATAATCAGCAGGTGAAACTGTCGATGCCGGAACTGGAAGAACTGGCGGCAGCAATGGTGCA
GGCGCAGGTCGATCGCAACGATGAGATTTATCGCCGTCAGCGTGAAATGAAAGAGGAGCTGAGCGGTCTGGATGATTTGG
CTTCAATTCGGGCGTTTGACGTTGAGTAA

Upstream 100 bases:

>100_bases
GAAAAGTGCAAATATAAAGAGGGGTTCCAGTCAGTAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGT
AAGAATCCGGAAACGCTCAC

Downstream 100 bases:

>100_bases
TGAATAAGCCGCAACTGGCGGAATCACAGAAGACCGCTTTGCTTACCGAAGCGGAGTCTGTCATCCGGCCGCGGGGCGTG
CTGTCAGGCTGAACAGGGAA

Product: transposase (IS4 family)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 542; Mature: 542

Protein sequence:

>542_residues
MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAESGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVN
RLDIMLPGNRPFVAPSAVIQARQRLGSEAVRRVFTKTAQLWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTH
AGNPALHPQVKMVCQMELTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGYYSLGLLNAWSLAGEHRHWMIPL
RKGAQYEELRKLGKGDHLVKLKTSPQARKKWPGLGNEVTARLLTVTRKGKVCHLLTSMTDAMRFPGGEMADLYSHRWEIE
LGYREIKQTMQLSRLTLRSKKPELVEQELWGVLLAYNLVRYQMIKMAESGAVDCDVFFDDRDQAVPYTATADDVAPTGQQ
IWQELQSGKWGEIAPFTVTPEMLEAAREARRQEIEAWRAEQEAKPFTFEWNGRIWNAGPDSLGRLSPVVMLAKSVTAQTH
MAWSDADNQQVKLSMPELEELAAAMVQAQVDRNDEIYRRQREMKEELSGLDDLASIRAFDVE

Sequences:

>Translated_542_residues
MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAESGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVN
RLDIMLPGNRPFVAPSAVIQARQRLGSEAVRRVFTKTAQLWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTH
AGNPALHPQVKMVCQMELTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGYYSLGLLNAWSLAGEHRHWMIPL
RKGAQYEELRKLGKGDHLVKLKTSPQARKKWPGLGNEVTARLLTVTRKGKVCHLLTSMTDAMRFPGGEMADLYSHRWEIE
LGYREIKQTMQLSRLTLRSKKPELVEQELWGVLLAYNLVRYQMIKMAESGAVDCDVFFDDRDQAVPYTATADDVAPTGQQ
IWQELQSGKWGEIAPFTVTPEMLEAAREARRQEIEAWRAEQEAKPFTFEWNGRIWNAGPDSLGRLSPVVMLAKSVTAQTH
MAWSDADNQQVKLSMPELEELAAAMVQAQVDRNDEIYRRQREMKEELSGLDDLASIRAFDVE
>Mature_542_residues
MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAESGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVN
RLDIMLPGNRPFVAPSAVIQARQRLGSEAVRRVFTKTAQLWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTH
AGNPALHPQVKMVCQMELTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGYYSLGLLNAWSLAGEHRHWMIPL
RKGAQYEELRKLGKGDHLVKLKTSPQARKKWPGLGNEVTARLLTVTRKGKVCHLLTSMTDAMRFPGGEMADLYSHRWEIE
LGYREIKQTMQLSRLTLRSKKPELVEQELWGVLLAYNLVRYQMIKMAESGAVDCDVFFDDRDQAVPYTATADDVAPTGQQ
IWQELQSGKWGEIAPFTVTPEMLEAAREARRQEIEAWRAEQEAKPFTFEWNGRIWNAGPDSLGRLSPVVMLAKSVTAQTH
MAWSDADNQQVKLSMPELEELAAAMVQAQVDRNDEIYRRQREMKEELSGLDDLASIRAFDVE

Specific function: Involved in the transposition of the insertion sequence IS4 [H]

COG id: COG3385

COG function: function code L; FOG: Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 11 family [H]

Homologues:

Organism=Escherichia coli, GI1790729, Length=362, Percent_Identity=98.8950276243094, Blast_Score=745, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012337
- InterPro:   IPR002559 [H]

Pfam domain/function: PF01609 Transposase_11 [H]

EC number: NA

Molecular weight: Translated: 61590; Mature: 61590

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAESGTVTLRKRRLPLEMM
CCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCHHHHHHHHHCCCCEEEHHHCCCHHHH
VWCIVGMALERKEPLHQIVNRLDIMLPGNRPFVAPSAVIQARQRLGSEAVRRVFTKTAQL
HHHHHHHHHHCCCHHHHHHHHHEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
WHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTHAGNPALHPQVKMVCQMELTS
HCCCCCCCCHHHHHHHHHCCEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
HLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGYYSLGLLNAWSLAGEHRHWMIPL
HHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCCCEEEEEC
RKGAQYEELRKLGKGDHLVKLKTSPQARKKWPGLGNEVTARLLTVTRKGKVCHLLTSMTD
CCCCCHHHHHHCCCCCCEEEEECCCHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHH
AMRFPGGEMADLYSHRWEIELGYREIKQTMQLSRLTLRSKKPELVEQELWGVLLAYNLVR
HHHCCCCCHHHHHHCCEEEEECHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
YQMIKMAESGAVDCDVFFDDRDQAVPYTATADDVAPTGQQIWQELQSGKWGEIAPFTVTP
HHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCEECH
EMLEAAREARRQEIEAWRAEQEAKPFTFEWNGRIWNAGPDSLGRLSPVVMLAKSVTAQTH
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCEEECCCCCCHHHHHHHHHHHHHHHHHHH
MAWSDADNQQVKLSMPELEELAAAMVQAQVDRNDEIYRRQREMKEELSGLDDLASIRAFD
EEECCCCCCEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCC
VE
CC
>Mature Secondary Structure
MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAESGTVTLRKRRLPLEMM
CCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCHHHHHHHHHCCCCEEEHHHCCCHHHH
VWCIVGMALERKEPLHQIVNRLDIMLPGNRPFVAPSAVIQARQRLGSEAVRRVFTKTAQL
HHHHHHHHHHCCCHHHHHHHHHEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
WHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTHAGNPALHPQVKMVCQMELTS
HCCCCCCCCHHHHHHHHHCCEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
HLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGYYSLGLLNAWSLAGEHRHWMIPL
HHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCCCEEEEEC
RKGAQYEELRKLGKGDHLVKLKTSPQARKKWPGLGNEVTARLLTVTRKGKVCHLLTSMTD
CCCCCHHHHHHCCCCCCEEEEECCCHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHH
AMRFPGGEMADLYSHRWEIELGYREIKQTMQLSRLTLRSKKPELVEQELWGVLLAYNLVR
HHHCCCCCHHHHHHCCEEEEECHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
YQMIKMAESGAVDCDVFFDDRDQAVPYTATADDVAPTGQQIWQELQSGKWGEIAPFTVTP
HHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCEECH
EMLEAAREARRQEIEAWRAEQEAKPFTFEWNGRIWNAGPDSLGRLSPVVMLAKSVTAQTH
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCEEECCCCCCHHHHHHHHHHHHHHHHHHH
MAWSDADNQQVKLSMPELEELAAAMVQAQVDRNDEIYRRQREMKEELSGLDDLASIRAFD
EEECCCCCCEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCC
VE
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6268937; 7610040; 9278503 [H]