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The map label for this gene is hofN

Identifier: 187730156

GI number: 187730156

Start: 3495692

End: 3496231

Strand: Reverse

Name: hofN

Synonym: SbBS512_E3771

Alternate gene names: 187730156

Gene position: 3496231-3495692 (Counterclockwise)

Preceding gene: 187731732

Following gene: 187734030

Centisome position: 75.74

GC content: 55.56

Gene sequence:

>540_bases
ATGAACCCGCCAATTAATTTTTTGCCCTGGCGACAGCAACGCCGGACCGCTTTTCTGCGTTTCTGGTTGCTGATGTTCGT
TGCGCCTCTGCTGCTGGCCGTCGGGATAACGCTAATACTGCGTCTGACAGGCAGCGCCGAAGCGCGCATAGACGCAGTTT
TGCTTCAGGCGGAACAACAACTCGCCCGCAGCTTACAGATAACGAAGCCACGTTTGCTGGAGCAACAGCAGTTACGCGAA
CAGCGTTCGCAACGGCAGCGCCAGCGACAATTTACCCGCGACTGGCAATCTGCGCTGGAAGCACTGGCGGCGCTTTTACC
CGAGCACGCCTGGCTGACAACGATAAGCTGGCAGCAGGGAACGCTGGAGATCAAGGGGCTGACAACAAGCATTACCGCGT
TAAACGCACTAGAAACGTCACTCCGCCAGGATGCGTCTTTTCATCTCAATCAGCGGGGAGCCACGCAGCAGGATGCGCAG
GGACGCTGGCAATTTGAGTATCAGTTAACAAGGAAGGTTAGCGATGAACATGTTCTTTGA

Upstream 100 bases:

>100_bases
TTGACCCCTGGGAGGCCGTTTCTGTTCGTCAGCCGCTGCTACCGCCGCCTGGTGGAGACTTTGCCATCGCGCTGGGGCTG
GCGCTTGGGAAGGCGTACTG

Downstream 100 bases:

>100_bases
CTGGTGGTTCGCCACATCACCCCGCCTCCGCCAGTTTTGCTGGGCATTCTGGTTGCTGATGTTAGTTACGCTCATTTTTC
TGTCATCGACACACCATGAA

Product: PilN family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 179; Mature: 179

Protein sequence:

>179_residues
MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTGSAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLRE
QRSQRQRQRQFTRDWQSALEALAALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
GRWQFEYQLTRKVSDEHVL

Sequences:

>Translated_179_residues
MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTGSAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLRE
QRSQRQRQRQFTRDWQSALEALAALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
GRWQFEYQLTRKVSDEHVL
>Mature_179_residues
MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTGSAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLRE
QRSQRQRQRQFTRDWQSALEALAALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
GRWQFEYQLTRKVSDEHVL

Specific function: Required for the use of extracellular DNA as a nutrient

COG id: COG3166

COG function: function code NU; Tfp pilus assembly protein PilN

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789796, Length=179, Percent_Identity=100, Blast_Score=352, Evalue=1e-98,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HOFN_ECOLI (P64634)

Other databases:

- EMBL:   U18997
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   E65134
- RefSeq:   AP_004396.1
- RefSeq:   NP_417853.1
- ProteinModelPortal:   P64634
- STRING:   P64634
- EnsemblBacteria:   EBESCT00000003111
- EnsemblBacteria:   EBESCT00000017166
- GeneID:   947898
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW3357
- KEGG:   eco:b3394
- EchoBASE:   EB2760
- EcoGene:   EG12924
- eggNOG:   COG3166
- GeneTree:   EBGT00050000011653
- HOGENOM:   HBG511441
- OMA:   FFHLNQR
- ProtClustDB:   CLSK880645
- BioCyc:   EcoCyc:G7738-MONOMER
- Genevestigator:   P64634
- InterPro:   IPR007813

Pfam domain/function: PF05137 PilN

EC number: NA

Molecular weight: Translated: 20796; Mature: 20796

Theoretical pI: Translated: 11.48; Mature: 11.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x211421b8)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTGSAEARIDAVLLQAEQQ
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
LARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEALAALLPEHAWLTTISWQQG
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEECCCC
TLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQGRWQFEYQLTRKVSDEHVL
EEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEHHHHHHHHCCCCCCH
>Mature Secondary Structure
MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTGSAEARIDAVLLQAEQQ
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
LARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEALAALLPEHAWLTTISWQQG
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEECCCC
TLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQGRWQFEYQLTRKVSDEHVL
EEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEHHHHHHHHCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9278503