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The map label for this gene is ynjE [H]

Identifier: 187730093

GI number: 187730093

Start: 1828799

End: 1830085

Strand: Direct

Name: ynjE [H]

Synonym: SbBS512_E2006

Alternate gene names: 187730093

Gene position: 1828799-1830085 (Clockwise)

Preceding gene: 187731872

Following gene: 187730434

Centisome position: 39.62

GC content: 53.61

Gene sequence:

>1287_bases
ATGAAACGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGC
GAAGCCTCTTACACTTGACCAGCTTCAACAACAAAATGGCAAAGCGATAGATACTCGCCCCAGCGCGTTTTATAACGGCT
GGCCACAAACCTTAAATGGCCCTTCTGGTCATGAACCTGCCGCCTTAAACCTCTCTGCCAGCTGGCTCGACAAAATGAGC
ACCGAACAGCTCAACGCGTGGATCAAGCAACATAACCTGAAAGCCGATGCCCCGGTGGCGCTGTACGGTAATGACAAAGA
TGTCGACGCCGTCAAAACGCGACTGCAAAAAGCAGGTTTTACGCATATCTCCATTCTGAGTGACGCGCTAAGCGAACCTT
CCCGTCTACAAAAACTGCCGCACTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTAAAGAGGTT
ACGGCGAAACCTGCCGGTGACTGGAAAGTCATTGAAGCGGCCTGGGGCGCTCCTAAGCTTTACCTTATCAGCCATATTCC
CGGTGCTGACTACATCGATACCAACGAAGTGGAAAGCGAACCGCTGTGGAACAAAGTTTCTGATGAACAACTAAAAGCGA
TGCTGGCAAAACACGGCATTCGCCATGACACCACGGTCATTCTGTACGGGCGTGACGTATACGCTGGAGCGCGTGTGGCG
CAAATTATGCTCTACGCAGGCGTGAAAGATGTGCGTCTGCTGGATGGCGGCTGGCAAACCTGGTCCGACGCGGGACTGCC
TGTTGAGCGAGGAACGCCACCGAAAGTGAAAGCGGAACCGGATTTCGGCGTGAAGATCCCGGCACAACCGCAGTTGATGC
TCGATATGGAACAGGCCCGTGGACTGCTGCATCGCCAGGATGCATCGCTGGTGAGCATTCGTTCGTGGCCAGAATTTATC
GGTACGACCAGCGGTTACAGCTATATTAAACCAAAAGGTGAAATAGCCGGAGCACGTTGGGGACACGCTGGTAGCGACTC
GACGCATATGGAAGATTTTCATAACCCGGATGGCACCATGCGCAGCGCCGATGATATTACCGCTATGTGGAAAGCGTGGA
ATATCAAACCAGAGCAGCAAGTTTCATTCTACTGCGGCACCGGCTGGCGCGCGTCCGAAACCTTTATGTACGCACGCGCC
ATGGGTTGGAAGAATGTTTCCGTGTATGACGGCGGCTGGTACGAATGGAGCAGCGATCCAAAAAATCCGGTAGCAACCGG
TGGGTAA

Upstream 100 bases:

>100_bases
GCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAA
CTACAATGTCGGGATTTTCG

Downstream 100 bases:

>100_bases
TGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAAC
GACAGCGACTTCCGTCCCAG

Product: rhodanese-like domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAIDTRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMS
TEQLNAWIKQHNLKADAPVALYGNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKHGIRHDTTVILYGRDVYAGARVA
QIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKVKAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFI
GTTSGYSYIKPKGEIAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRASETFMYARA
MGWKNVSVYDGGWYEWSSDPKNPVATGG

Sequences:

>Translated_428_residues
MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAIDTRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMS
TEQLNAWIKQHNLKADAPVALYGNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKHGIRHDTTVILYGRDVYAGARVA
QIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKVKAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFI
GTTSGYSYIKPKGEIAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRASETFMYARA
MGWKNVSVYDGGWYEWSSDPKNPVATGG
>Mature_428_residues
MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAIDTRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMS
TEQLNAWIKQHNLKADAPVALYGNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKHGIRHDTTVILYGRDVYAGARVA
QIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKVKAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFI
GTTSGYSYIKPKGEIAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRASETFMYARA
MGWKNVSVYDGGWYEWSSDPKNPVATGG

Specific function: Unknown

COG id: COG2897

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 rhodanese domains [H]

Homologues:

Organism=Homo sapiens, GI17402865, Length=301, Percent_Identity=26.2458471760797, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI61835204, Length=300, Percent_Identity=24.3333333333333, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI194473681, Length=300, Percent_Identity=24.3333333333333, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI194473668, Length=300, Percent_Identity=24.3333333333333, Blast_Score=70, Evalue=4e-12,
Organism=Escherichia coli, GI87081967, Length=427, Percent_Identity=99.0632318501171, Blast_Score=872, Evalue=0.0,
Organism=Escherichia coli, GI87082121, Length=249, Percent_Identity=22.0883534136546, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR001307 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: =2.8.1.1 [H]

Molecular weight: Translated: 47403; Mature: 47403

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00380 RHODANESE_1 ; PS00683 RHODANESE_2 ; PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAIDTRPSAFYNGWPQTLNG
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEECCCCCHHHCCCCCCCCC
PSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALYGNDKDVDAVKTRLQKAGF
CCCCCCEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHCCC
THISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEVTAKPAGDWKVIEAAWGAPKL
HHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHCCCEEECCCCCCEEEEEECCCCCEE
YLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKHGIRHDTTVILYGRDVYAGARVA
EEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHH
QIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKVKAEPDFGVKIPAQPQLMLDMEQAR
HHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCEECCCCCCEECCCCCHHEEEHHHHC
GLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGEIAGARWGHAGSDSTHMEDFHNPDGTM
CHHHHCCCCEEEEHHCHHHHCCCCCCCEECCCCCCCCCCCCCCCCCCCCHHHHCCCCCCC
RSADDITAMWKAWNIKPEQQVSFYCGTGWRASETFMYARAMGWKNVSVYDGGWYEWSSDP
CCCHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCEEEECCCEEECCCCC
KNPVATGG
CCCCCCCC
>Mature Secondary Structure
MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAIDTRPSAFYNGWPQTLNG
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEECCCCCHHHCCCCCCCCC
PSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALYGNDKDVDAVKTRLQKAGF
CCCCCCEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHCCC
THISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEVTAKPAGDWKVIEAAWGAPKL
HHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHCCCEEECCCCCCEEEEEECCCCCEE
YLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKHGIRHDTTVILYGRDVYAGARVA
EEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHH
QIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKVKAEPDFGVKIPAQPQLMLDMEQAR
HHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCEECCCCCCEECCCCCHHEEEHHHHC
GLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGEIAGARWGHAGSDSTHMEDFHNPDGTM
CHHHHCCCCEEEEHHCHHHHCCCCCCCEECCCCCCCCCCCCCCCCCCCCHHHHCCCCCCC
RSADDITAMWKAWNIKPEQQVSFYCGTGWRASETFMYARAMGWKNVSVYDGGWYEWSSDP
CCCHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCEEEECCCEEECCCCC
KNPVATGG
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 9097039 [H]