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The map label for this gene is rfaY

Identifier: 187730092

GI number: 187730092

Start: 3785234

End: 3785926

Strand: Reverse

Name: rfaY

Synonym: SbBS512_E4052

Alternate gene names: 187730092

Gene position: 3785926-3785234 (Counterclockwise)

Preceding gene: 187730561

Following gene: 187733640

Centisome position: 82.02

GC content: 35.06

Gene sequence:

>693_bases
ATGATTACAAGTATACGTTATCGCGGCTTCTCATTTTATTACAAAGATAATGATAATAAATATAAAGAAATCTTTGATGA
AATACTGGCCTATAATTTTAAAACAGTAAAAGTATTATGTAATATTGATGATACCAAAGTATCGTTGATTGATACGAAAT
ATGGTCGCTATGTTTTTAAAGTCTTTGCTCCGAAAACGAAAAGGAATGAACGTTTTTTAAAATCTTTTGTAAAAGGTGAC
TACTATCAGAATTTGATCGTTGAAACCGATCGTGTCATAAGTGCAGGTCTTACTTTTCCTAATGATTTTTATTTTCTGGC
TGGGCGTAAAATTTTCAACTATGCCAGTGTTTTTATCATGCTTATCGAGTATGTCGAGGGCGTAGAGCTCAATGATATGC
CGATTATTCCAGAAAATGTTAAAGCAGAAATTAAGGCTTCAATGGAAAAATTGCATGCCTTAAATATGCTATCGGGCGAC
CCACACCGGGGTAACTTTATCGTTAGTAAAGATGGTGTCAGGATAATTGACCTATCCGGTAAAAGCTGTACGGCAGAGCG
TAAAGCTCGTGATCGTCTGGCCATGGAACGTCACCTGGGTATTGCCAACGAAATAAAAGATTATGGCTACTATTCAGTAA
TCTACAGAACCAAACTACGCAAATTTATTAAAAAATTAAAAGGCAAAGCGTAA

Upstream 100 bases:

>100_bases
AGAAATGCAGAAACAATATAAGCATCTGTTTGCCCATGGTGAGTATATAAAAGGCATAACTTCATTAATTAAGTACAAGC
TTAAGAAATAAATTACTCTT

Downstream 100 bases:

>100_bases
ACCACACAGTCAAAACGGAACCAACATGGATTTATTAGCTGAGAGTATTACTGAAGTCGCTGTCTCTGGGGAAATTGCTA
ACACCGATCGTGTGTTAAAT

Product: lipopolysaccharide core biosynthesis protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 230; Mature: 230

Protein sequence:

>230_residues
MITSIRYRGFSFYYKDNDNKYKEIFDEILAYNFKTVKVLCNIDDTKVSLIDTKYGRYVFKVFAPKTKRNERFLKSFVKGD
YYQNLIVETDRVISAGLTFPNDFYFLAGRKIFNYASVFIMLIEYVEGVELNDMPIIPENVKAEIKASMEKLHALNMLSGD
PHRGNFIVSKDGVRIIDLSGKSCTAERKARDRLAMERHLGIANEIKDYGYYSVIYRTKLRKFIKKLKGKA

Sequences:

>Translated_230_residues
MITSIRYRGFSFYYKDNDNKYKEIFDEILAYNFKTVKVLCNIDDTKVSLIDTKYGRYVFKVFAPKTKRNERFLKSFVKGD
YYQNLIVETDRVISAGLTFPNDFYFLAGRKIFNYASVFIMLIEYVEGVELNDMPIIPENVKAEIKASMEKLHALNMLSGD
PHRGNFIVSKDGVRIIDLSGKSCTAERKARDRLAMERHLGIANEIKDYGYYSVIYRTKLRKFIKKLKGKA
>Mature_230_residues
MITSIRYRGFSFYYKDNDNKYKEIFDEILAYNFKTVKVLCNIDDTKVSLIDTKYGRYVFKVFAPKTKRNERFLKSFVKGD
YYQNLIVETDRVISAGLTFPNDFYFLAGRKIFNYASVFIMLIEYVEGVELNDMPIIPENVKAEIKASMEKLHALNMLSGD
PHRGNFIVSKDGVRIIDLSGKSCTAERKARDRLAMERHLGIANEIKDYGYYSVIYRTKLRKFIKKLKGKA

Specific function: Catalyzes the phosphorylation of heptose(II) of the outer membrane lipopolysaccharide core [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the protein kinase superfamily. RfaY/waaY family [H]

Homologues:

Organism=Escherichia coli, GI1790055, Length=230, Percent_Identity=50.8695652173913, Blast_Score=249, Evalue=9e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011009
- InterPro:   IPR009330 [H]

Pfam domain/function: PF06176 WaaY [H]

EC number: 2.7.-.-

Molecular weight: Translated: 26827; Mature: 26827

Theoretical pI: Translated: 9.96; Mature: 9.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITSIRYRGFSFYYKDNDNKYKEIFDEILAYNFKTVKVLCNIDDTKVSLIDTKYGRYVFK
CCCEEEECCEEEEEECCCCHHHHHHHHHHHCCCEEEEEEEECCCCEEEEEECCCCCEEEE
VFAPKTKRNERFLKSFVKGDYYQNLIVETDRVISAGLTFPNDFYFLAGRKIFNYASVFIM
EECCCCCCHHHHHHHHHCCHHHHHHHHCCCHHHCCCCCCCCCEEEECCCHHHHHHHHHHH
LIEYVEGVELNDMPIIPENVKAEIKASMEKLHALNMLSGDPHRGNFIVSKDGVRIIDLSG
HHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCEEEEEECC
KSCTAERKARDRLAMERHLGIANEIKDYGYYSVIYRTKLRKFIKKLKGKA
CCCCHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MITSIRYRGFSFYYKDNDNKYKEIFDEILAYNFKTVKVLCNIDDTKVSLIDTKYGRYVFK
CCCEEEECCEEEEEECCCCHHHHHHHHHHHCCCEEEEEEEECCCCEEEEEECCCCCEEEE
VFAPKTKRNERFLKSFVKGDYYQNLIVETDRVISAGLTFPNDFYFLAGRKIFNYASVFIM
EECCCCCCHHHHHHHHHCCHHHHHHHHCCCHHHCCCCCCCCCEEEECCCHHHHHHHHHHH
LIEYVEGVELNDMPIIPENVKAEIKASMEKLHALNMLSGDPHRGNFIVSKDGVRIIDLSG
HHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCEEEEEECC
KSCTAERKARDRLAMERHLGIANEIKDYGYYSVIYRTKLRKFIKKLKGKA
CCCCHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA