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The map label for this gene is yfiE

Identifier: 187730042

GI number: 187730042

Start: 2765918

End: 2766310

Strand: Direct

Name: yfiE

Synonym: SbBS512_E2960

Alternate gene names: 187730042

Gene position: 2765918-2766310 (Clockwise)

Preceding gene: 187732592

Following gene: 187733617

Centisome position: 59.92

GC content: 49.11

Gene sequence:

>393_bases
ATGGATCTGCGCCGTTTTATTACGCTTAAAACCGTGGTGGAAGAGGGTTCCTTTTTGCGAGCTTCGCAAAAATTGTGCTG
TACACAATCGACGGTGACTTTTCATATTCAGCAGCTTGAGCAGGAGTTTTCAGTCCAGTTATTTGAGAAAATTGGCCGAC
GGATGTGCCTTACCCGCGAAGGAAAAAAGTTGCTGCCGCACATTTATGAACTTACCCGAGTGATGGATACGCTTCGTGAG
GCTGCCAAAAAGGAGTCAGATCCGGACGGGGAACTACGCGTTGTTTCGGGCGAAACGTTGCTCTCTTACCGAATGCCGCA
GGTGTTGCAGCGTTTTCGGCAACGCGCACCAAAAGTGCGTTTATCCTTGCAGTCGCTGAACTGTTACGTGTAA

Upstream 100 bases:

>100_bases
GTCTGGAAATATAGGGGCAAATCCAGTACCCGACACAGAACATTTAAAAAAATGAATGTTTTTATATTCTAGATTCGATA
TTTTCGATAGGTTTGGGGTT

Downstream 100 bases:

>100_bases
GCGTCAACGGAGCACCGTATTGACGCTTATTTATTGGTGAGTGCTACGTTCCATGGCAGGAGTTCGTCAACACGGTTGGA
AGGCCATTCCGGCAGTACGC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 130; Mature: 130

Protein sequence:

>130_residues
MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFSVQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLRE
AAKKESDPDGELRVVSGETLLSYRMPQVLQRFRQRAPKVRLSLQSLNCYV

Sequences:

>Translated_130_residues
MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFSVQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLRE
AAKKESDPDGELRVVSGETLLSYRMPQVLQRFRQRAPKVRLSLQSLNCYV
>Mature_130_residues
MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFSVQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLRE
AAKKESDPDGELRVVSGETLLSYRMPQVLQRFRQRAPKVRLSLQSLNCYV

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI87082132, Length=130, Percent_Identity=100, Blast_Score=265, Evalue=8e-73,
Organism=Escherichia coli, GI1788481, Length=131, Percent_Identity=28.2442748091603, Blast_Score=66, Evalue=6e-13,
Organism=Escherichia coli, GI1786401, Length=123, Percent_Identity=34.9593495934959, Blast_Score=61, Evalue=2e-11,
Organism=Escherichia coli, GI1787806, Length=125, Percent_Identity=29.6, Blast_Score=61, Evalue=3e-11,
Organism=Escherichia coli, GI145693105, Length=120, Percent_Identity=30, Blast_Score=59, Evalue=8e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): YFIE_ECOLI (P33634)

Other databases:

- EMBL:   D13169
- EMBL:   D64044
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   H65035
- RefSeq:   AP_003163.1
- RefSeq:   NP_417072.4
- ProteinModelPortal:   P33634
- STRING:   P33634
- EnsemblBacteria:   EBESCT00000002013
- EnsemblBacteria:   EBESCT00000015507
- GeneID:   947064
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW2561
- KEGG:   eco:b2577
- EchoBASE:   EB1733
- EcoGene:   EG11785
- eggNOG:   COG0583
- GeneTree:   EBGT00070000031692
- HOGENOM:   HBG685425
- OMA:   VKELECG
- ProtClustDB:   CLSK880438
- BioCyc:   EcoCyc:EG11785-MONOMER
- Genevestigator:   P33634
- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate

EC number: NA

Molecular weight: Translated: 15223; Mature: 15223

Theoretical pI: Translated: 9.93; Mature: 9.93

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFSVQLFEKIGRRMCLTRE
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLSYRMPQVLQRFRQRAPKVR
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCHHE
LSLQSLNCYV
EHHHHCCCCC
>Mature Secondary Structure
MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFSVQLFEKIGRRMCLTRE
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLSYRMPQVLQRFRQRAPKVR
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCHHE
LSLQSLNCYV
EHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 9205837