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The map label for this gene is rhtB [H]

Identifier: 186477836

GI number: 186477836

Start: 3471041

End: 3471667

Strand: Direct

Name: rhtB [H]

Synonym: Bphy_3092

Alternate gene names: 186477836

Gene position: 3471041-3471667 (Clockwise)

Preceding gene: 186477833

Following gene: 186477837

Centisome position: 99.77

GC content: 62.04

Gene sequence:

>627_bases
ATGACGCTTGCCCACTGGCTTCCATTTGCGGTCGCATCGGCGATTCTGGTCGCGATTCCGGGTCCTACCGTGCTTCTTGT
AATTTCTTATGCGCTCGGCCATGGACGGCGCTATGCGTTGGTAACCACAGCAGGCGTCGCGCTCGGCGATCTGACATCGA
TGACGGCGTCGATGCTGGGACTCGGCGTCATTCTCGCGGCATCCGCGGTGCTGTTCACGGCGATAAAGTGGGTAGGCGCC
GCATATCTGATTTACCTTGGCATCAAGTTGTGGCGCACGCCCGTATCTGCTGGCGAAGGGCCGCGGACAGGCGAAACGCG
CACGAGCCGTATTTTCGCGCACGCCTATGCCGTCACCGCGCTCAATCCGAAAAGCATTGTGTTCTTCGTGGCATTCGTGC
CCCAGTTCATCGATACGCAAGTCGCCACCTGGTCGCAGATCGCGGTTTTCGAGGCGACCTTCGTTGCGCTTGGCACGGTG
AACGCGCTCGGCTATGCCGTGTTGGCGTCGGCGGCCCGGCGCGCGATCCGCAGTCCGCGTGTACAGCGCAGCGTCAATTG
CACGGGAGGAACGCTGCTGATCGGCGCGGGGCTGCTGGCAGCGGCGTGGAAAAAATCGGCCGCATGA

Upstream 100 bases:

>100_bases
CGCCGTCTTCGTGGATAAATCGACCCGCCCCAACCAAAACTATTGCGAGACGGGTGCGAGGCCAGAAGCGTCCGCTACCG
TCGACAGGAGACTTGCTCTA

Downstream 100 bases:

>100_bases
GTTTCTTCTGCGGAGGACGACGACGATCCAGGCGCGCGGCTTGCGACTCGACTCGCGCACCGGGCGTGCGCGGTGGAGCA
CACGCAGTGGCTACCAACAC

Product: lysine exporter protein LysE/YggA

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 208; Mature: 207

Protein sequence:

>208_residues
MTLAHWLPFAVASAILVAIPGPTVLLVISYALGHGRRYALVTTAGVALGDLTSMTASMLGLGVILAASAVLFTAIKWVGA
AYLIYLGIKLWRTPVSAGEGPRTGETRTSRIFAHAYAVTALNPKSIVFFVAFVPQFIDTQVATWSQIAVFEATFVALGTV
NALGYAVLASAARRAIRSPRVQRSVNCTGGTLLIGAGLLAAAWKKSAA

Sequences:

>Translated_208_residues
MTLAHWLPFAVASAILVAIPGPTVLLVISYALGHGRRYALVTTAGVALGDLTSMTASMLGLGVILAASAVLFTAIKWVGA
AYLIYLGIKLWRTPVSAGEGPRTGETRTSRIFAHAYAVTALNPKSIVFFVAFVPQFIDTQVATWSQIAVFEATFVALGTV
NALGYAVLASAARRAIRSPRVQRSVNCTGGTLLIGAGLLAAAWKKSAA
>Mature_207_residues
TLAHWLPFAVASAILVAIPGPTVLLVISYALGHGRRYALVTTAGVALGDLTSMTASMLGLGVILAASAVLFTAIKWVGAA
YLIYLGIKLWRTPVSAGEGPRTGETRTSRIFAHAYAVTALNPKSIVFFVAFVPQFIDTQVATWSQIAVFEATFVALGTVN
ALGYAVLASAARRAIRSPRVQRSVNCTGGTLLIGAGLLAAAWKKSAA

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=203, Percent_Identity=34.4827586206897, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI1788099, Length=139, Percent_Identity=30.9352517985612, Blast_Score=79, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 21718; Mature: 21587

Theoretical pI: Translated: 11.06; Mature: 11.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLAHWLPFAVASAILVAIPGPTVLLVISYALGHGRRYALVTTAGVALGDLTSMTASMLG
CCHHHHHHHHHHHHHHEECCCCHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHH
LGVILAASAVLFTAIKWVGAAYLIYLGIKLWRTPVSAGEGPRTGETRTSRIFAHAYAVTA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHEEEE
LNPKSIVFFVAFVPQFIDTQVATWSQIAVFEATFVALGTVNALGYAVLASAARRAIRSPR
ECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VQRSVNCTGGTLLIGAGLLAAAWKKSAA
HHCCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TLAHWLPFAVASAILVAIPGPTVLLVISYALGHGRRYALVTTAGVALGDLTSMTASMLG
CHHHHHHHHHHHHHHEECCCCHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHH
LGVILAASAVLFTAIKWVGAAYLIYLGIKLWRTPVSAGEGPRTGETRTSRIFAHAYAVTA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHEEEE
LNPKSIVFFVAFVPQFIDTQVATWSQIAVFEATFVALGTVNALGYAVLASAARRAIRSPR
ECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VQRSVNCTGGTLLIGAGLLAAAWKKSAA
HHCCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]