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The map label for this gene is fliA [H]

Identifier: 186477706

GI number: 186477706

Start: 3315325

End: 3316059

Strand: Reverse

Name: fliA [H]

Synonym: Bphy_2960

Alternate gene names: 186477706

Gene position: 3316059-3315325 (Counterclockwise)

Preceding gene: 186477707

Following gene: 186477693

Centisome position: 95.31

GC content: 62.45

Gene sequence:

>735_bases
ATGTACAACGCTCAAGGAAAAATTTCGCAAGCCGACGTTTTGACGAAATACGCACCGCTCGTGCGTCGTCTCGGCTTGCA
GCTCGTCGCCAAGATGCCGGCGAGCGTCGATCTCGACGATCTGATTCAGGCGGGCATGATTGGCCTGCTGGACGCGGCGA
GCCGTTACAAGGAAGACCAGGGTGCGCAGTTCGAGACGTACGCGAGCCAGCGGATTCGCGGCGCGATGCTCGACGAGCTG
CGCAGCAACGACTGGCTGCCGCGTAGCTTGCGCCGCACGTCGCGCGAGGTCGAGAGTGCCGTGCATCAGGTCGAGCAGTC
GCTGGGCCGCTCGGCGAGTGAGACGGAGATCGCCGAGCATCTGAAGATGCCGCTCGACGAGTATCAGTCGATGCTGCAGG
ATCTGCACGGCAGCCAGTTGATCTACTACGAGGACTTCGATCGTTCGGCGGACGACGAGCCGTTCCTGGACCGCTATTGC
GTCGATCATTCGGATCCGCTGTCGGCGCTGCTGGACGAGTCGCTGCGGGGTGCTCTGGTCGAGGCGATCGACCGCTTGCC
GGAGCGGGAGAAGCTGCTGATGTCGCTGTACTATGAGCGCGGGTTGAACCTGCGCGAGATCGGGGCTGTGATGGAGGTGA
GCGAATCCCGCGTTTGTCAGCTGCATTCTCAGGCCGTTGCACGGCTTCGGACCCGGTTGCGCGAGCAGTCATGGGCTTCG
GCTGAGACGAGTTAA

Upstream 100 bases:

>100_bases
CACGGACGCATGGCGTGCCGCCGACGACCGTAACAGCGGCGCAATTTGCCGATCAACCGTCTGCGCAGCACGCCTGAAGG
CAAGGAAAAAGGGGAGCACG

Downstream 100 bases:

>100_bases
TCTGTTTTTTGCCCTGCGGGCGGTAAATCGTTTCTTCGAGAGCCGATCTTTTTTAAGATCGGCTCTTTGGTTTTGGTTTG
AATAGCGGTTCTGGTTTGGC

Product: flagellar biosynthesis sigma factor

Products: NA

Alternate protein names: Sigma-27; Sigma-28; Sigma-F factor [H]

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MYNAQGKISQADVLTKYAPLVRRLGLQLVAKMPASVDLDDLIQAGMIGLLDAASRYKEDQGAQFETYASQRIRGAMLDEL
RSNDWLPRSLRRTSREVESAVHQVEQSLGRSASETEIAEHLKMPLDEYQSMLQDLHGSQLIYYEDFDRSADDEPFLDRYC
VDHSDPLSALLDESLRGALVEAIDRLPEREKLLMSLYYERGLNLREIGAVMEVSESRVCQLHSQAVARLRTRLREQSWAS
AETS

Sequences:

>Translated_244_residues
MYNAQGKISQADVLTKYAPLVRRLGLQLVAKMPASVDLDDLIQAGMIGLLDAASRYKEDQGAQFETYASQRIRGAMLDEL
RSNDWLPRSLRRTSREVESAVHQVEQSLGRSASETEIAEHLKMPLDEYQSMLQDLHGSQLIYYEDFDRSADDEPFLDRYC
VDHSDPLSALLDESLRGALVEAIDRLPEREKLLMSLYYERGLNLREIGAVMEVSESRVCQLHSQAVARLRTRLREQSWAS
AETS
>Mature_244_residues
MYNAQGKISQADVLTKYAPLVRRLGLQLVAKMPASVDLDDLIQAGMIGLLDAASRYKEDQGAQFETYASQRIRGAMLDEL
RSNDWLPRSLRRTSREVESAVHQVEQSLGRSASETEIAEHLKMPLDEYQSMLQDLHGSQLIYYEDFDRSADDEPFLDRYC
VDHSDPLSALLDESLRGALVEAIDRLPEREKLLMSLYYERGLNLREIGAVMEVSESRVCQLHSQAVARLRTRLREQSWAS
AETS

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This alternative sigma factor is specific for class 3 flagellar operons [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1788231, Length=235, Percent_Identity=54.0425531914894, Blast_Score=247, Evalue=6e-67,
Organism=Escherichia coli, GI1789448, Length=210, Percent_Identity=27.6190476190476, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR012845
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 27739; Mature: 27739

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYNAQGKISQADVLTKYAPLVRRLGLQLVAKMPASVDLDDLIQAGMIGLLDAASRYKEDQ
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
GAQFETYASQRIRGAMLDELRSNDWLPRSLRRTSREVESAVHQVEQSLGRSASETEIAEH
CCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
LKMPLDEYQSMLQDLHGSQLIYYEDFDRSADDEPFLDRYCVDHSDPLSALLDESLRGALV
HCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHH
EAIDRLPEREKLLMSLYYERGLNLREIGAVMEVSESRVCQLHSQAVARLRTRLREQSWAS
HHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
AETS
CCCC
>Mature Secondary Structure
MYNAQGKISQADVLTKYAPLVRRLGLQLVAKMPASVDLDDLIQAGMIGLLDAASRYKEDQ
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
GAQFETYASQRIRGAMLDELRSNDWLPRSLRRTSREVESAVHQVEQSLGRSASETEIAEH
CCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
LKMPLDEYQSMLQDLHGSQLIYYEDFDRSADDEPFLDRYCVDHSDPLSALLDESLRGALV
HCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHH
EAIDRLPEREKLLMSLYYERGLNLREIGAVMEVSESRVCQLHSQAVARLRTRLREQSWAS
HHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
AETS
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]