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The map label for this gene is fliM [H]

Identifier: 186477685

GI number: 186477685

Start: 3294911

End: 3295909

Strand: Direct

Name: fliM [H]

Synonym: Bphy_2938

Alternate gene names: 186477685

Gene position: 3294911-3295909 (Clockwise)

Preceding gene: 186477684

Following gene: 186477686

Centisome position: 94.7

GC content: 60.16

Gene sequence:

>999_bases
ATGGGCCACGAAGAGTTCATGTCCCAGGAGGAGGTCGATGCCCTTCTCAAGGGCGTCACCGGCGAGTCTGACTCGCAAGC
CGACCAGTCCGATCGTGCCGGCGTCCGCCCGTACAACATCGCGACACAGGAGCGCATCGTTCGCGGCCGGATGCCCGGCC
TCGAAATCATCAACGACCGTTTCGCGCGACTGTTGCGCGTCGGCATTTTCAACTTCATGCGGCGCACAGCGGAAATTTCG
GTCGGCCCGGTGAAGGTGCAGAAGTACAGCGAATTCACGCGCAATCTGCCGATCCCGACGAACCTGAACCTCGTCCATGT
GAAGCCTCTGCGCGGCACGTCGCTGTTCGTGTTCGATCCGAACCTCGTGTTCTTCGTGGTCGACAATCTGTTCGGCGGCG
ACGGGCGTTTTCATACGCGCGTCGAAGGCCGTGAATTCACGCAGACCGAGCAGCGCATCATCCACAAGCTGCTGAATCTC
GTGTTCGAACACTATACGGCTGCATGGAAGAGCGTGCGGCCGCTGTCGTTCGAATACACGCGCTCGGAAATGCATACGCA
GTTCGCAAACGTTGCCACGCCGAACGAGATCGTGATCGTCACGCAGTTCTCGATCGAATTCGGACCGACGGGCGGCACGC
TGCATATCTGCATGCCGTACTCGATGATCGAGCCGATCCGCGACGTGCTCTCCTCGCCGATTCAGGGCGAAGCGCTCGAA
GTGGACCGCCGCTGGGTGCGCGTGCTGTCGCAGCAGGTGCAGGCAGCCGAAGTAGAGCTGACGGCGGATCTCGCGCAGAT
CCCCGTGACGTTCCAGGAGATTCTCAACATGAAGGCGGGCGACGTGCTGCCCATCAATATTCCGGAGCACATCACCGCGA
AAGTCGACGGCGTGCCCGTGATGGAATGCGGCTACGGTATTTTCAATGGGCAATACGCGCTGCGCGTGCAGAAGATGATC
AGCGCAGCGGAACAGATGAAGGAAGCGGGATATGACTGA

Upstream 100 bases:

>100_bases
TCGGACGTGCTGTTCACTGAATTCGTCGTCCAGTAACGCGCCTAGCGCTGACTGATTTGATCTTGAACCGCGCCAACGAA
GGGACGCACGAGGAATAGGA

Downstream 100 bases:

>100_bases
GTTGAACTCGACACCCGAAATGGACATGCCGGAAGAGTCCAAAGTGAATGATTCGATGCCTGGCTCGGGCTCCGACGAAG
CATCGCTCGACGATTGGGCG

Product: flagellar motor switch protein FliM

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MGHEEFMSQEEVDALLKGVTGESDSQADQSDRAGVRPYNIATQERIVRGRMPGLEIINDRFARLLRVGIFNFMRRTAEIS
VGPVKVQKYSEFTRNLPIPTNLNLVHVKPLRGTSLFVFDPNLVFFVVDNLFGGDGRFHTRVEGREFTQTEQRIIHKLLNL
VFEHYTAAWKSVRPLSFEYTRSEMHTQFANVATPNEIVIVTQFSIEFGPTGGTLHICMPYSMIEPIRDVLSSPIQGEALE
VDRRWVRVLSQQVQAAEVELTADLAQIPVTFQEILNMKAGDVLPINIPEHITAKVDGVPVMECGYGIFNGQYALRVQKMI
SAAEQMKEAGYD

Sequences:

>Translated_332_residues
MGHEEFMSQEEVDALLKGVTGESDSQADQSDRAGVRPYNIATQERIVRGRMPGLEIINDRFARLLRVGIFNFMRRTAEIS
VGPVKVQKYSEFTRNLPIPTNLNLVHVKPLRGTSLFVFDPNLVFFVVDNLFGGDGRFHTRVEGREFTQTEQRIIHKLLNL
VFEHYTAAWKSVRPLSFEYTRSEMHTQFANVATPNEIVIVTQFSIEFGPTGGTLHICMPYSMIEPIRDVLSSPIQGEALE
VDRRWVRVLSQQVQAAEVELTADLAQIPVTFQEILNMKAGDVLPINIPEHITAKVDGVPVMECGYGIFNGQYALRVQKMI
SAAEQMKEAGYD
>Mature_331_residues
GHEEFMSQEEVDALLKGVTGESDSQADQSDRAGVRPYNIATQERIVRGRMPGLEIINDRFARLLRVGIFNFMRRTAEISV
GPVKVQKYSEFTRNLPIPTNLNLVHVKPLRGTSLFVFDPNLVFFVVDNLFGGDGRFHTRVEGREFTQTEQRIIHKLLNLV
FEHYTAAWKSVRPLSFEYTRSEMHTQFANVATPNEIVIVTQFSIEFGPTGGTLHICMPYSMIEPIRDVLSSPIQGEALEV
DRRWVRVLSQQVQAAEVELTADLAQIPVTFQEILNMKAGDVLPINIPEHITAKVDGVPVMECGYGIFNGQYALRVQKMIS
AAEQMKEAGYD

Specific function: FliM is one of three proteins (FliG, FliN, FliM) that form the rotor-mounted switch complex (C ring), located at the base of the basal body. This complex interacts with the CheY and CheZ chemotaxis proteins, in addition to contacting components of the mot

COG id: COG1868

COG function: function code N; Flagellar motor switch protein

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein. Bacterial flagellum basal body [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FliM family [H]

Homologues:

Organism=Escherichia coli, GI1788255, Length=323, Percent_Identity=52.0123839009288, Blast_Score=352, Evalue=2e-98,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001689
- InterPro:   IPR001543 [H]

Pfam domain/function: PF02154 FliM; PF01052 SpoA [H]

EC number: NA

Molecular weight: Translated: 37488; Mature: 37357

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGHEEFMSQEEVDALLKGVTGESDSQADQSDRAGVRPYNIATQERIVRGRMPGLEIINDR
CCHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHH
FARLLRVGIFNFMRRTAEISVGPVKVQKYSEFTRNLPIPTNLNLVHVKPLRGTSLFVFDP
HHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHCCCCCCCCCCEEEEECCCCCEEEEECC
NLVFFVVDNLFGGDGRFHTRVEGREFTQTEQRIIHKLLNLVFEHYTAAWKSVRPLSFEYT
CEEEEEEEHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
RSEMHTQFANVATPNEIVIVTQFSIEFGPTGGTLHICMPYSMIEPIRDVLSSPIQGEALE
HHHHHHHHHCCCCCCEEEEEEEEEEEECCCCCEEEEEECHHHHHHHHHHHCCCCCCCHHH
VDRRWVRVLSQQVQAAEVELTADLAQIPVTFQEILNMKAGDVLPINIPEHITAKVDGVPV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCEEEECCCCHHEEECCCCEE
MECGYGIFNGQYALRVQKMISAAEQMKEAGYD
HHCCCCEECCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
GHEEFMSQEEVDALLKGVTGESDSQADQSDRAGVRPYNIATQERIVRGRMPGLEIINDR
CHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHH
FARLLRVGIFNFMRRTAEISVGPVKVQKYSEFTRNLPIPTNLNLVHVKPLRGTSLFVFDP
HHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHCCCCCCCCCCEEEEECCCCCEEEEECC
NLVFFVVDNLFGGDGRFHTRVEGREFTQTEQRIIHKLLNLVFEHYTAAWKSVRPLSFEYT
CEEEEEEEHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
RSEMHTQFANVATPNEIVIVTQFSIEFGPTGGTLHICMPYSMIEPIRDVLSSPIQGEALE
HHHHHHHHHCCCCCCEEEEEEEEEEEECCCCCEEEEEECHHHHHHHHHHHCCCCCCCHHH
VDRRWVRVLSQQVQAAEVELTADLAQIPVTFQEILNMKAGDVLPINIPEHITAKVDGVPV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCEEEECCCCHHEEECCCCEE
MECGYGIFNGQYALRVQKMISAAEQMKEAGYD
HHCCCCEECCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 3519573; 9097040; 9278503; 2651416 [H]