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The map label for this gene is dosC [H]

Identifier: 186477632

GI number: 186477632

Start: 3238315

End: 3239265

Strand: Direct

Name: dosC [H]

Synonym: Bphy_2884

Alternate gene names: 186477632

Gene position: 3238315-3239265 (Clockwise)

Preceding gene: 186477629

Following gene: 186477633

Centisome position: 93.08

GC content: 66.77

Gene sequence:

>951_bases
ATGAAATCGATTCACGATCTGCGCACTGACGACATCGCCTGCGAAGCACTGCTGGGCGCGCTCGACGGCAGAACGCAGCT
GGTCGCCATCTACGACGCAAGCGACCGGCTGATGTTCGCGAACAGCGCGTTCAAGCATGCGTTCAGGCTCAACCGCGAGC
GCGGCGACATCACGTTCGCCGATCTCGTGCTGCGCGGGATTCTGGGGCAATGCGGGCCGCGCATCGGAGGCGACGACGCG
TCGGCCGTGATCGCCGATGCGATCGCCGCACGCCGCGAACGCGCCGGACAGCGCAGCTTCGCGACGGAGCTCGTCGACGG
ACGCTGGTTCTGGATGACGGAATCGCTGCTGGAGAACGACTGGATCGTGCTGACGGGTACCGAGATCTCGGCACTGCGCT
CCAACGAGCAGGCGTGGATCGGCGCGCACGAACGCGCGGTGCAGGAAGCGCGCACCGACGCGCTGACGGGCCTCGCGAAC
CGGCGCCATTCGATGTCGTCGCTCGAACTGGCGATCACGGCTGCTGCCGGCACCGAGACGCCGCTCACGGTCGCGCTCGT
CGACCTCGATCATTTCAAGCAGATCAACGATTCGCACGGGCACGCCGCAGGCGACGCGGTGCTGCGCGATTTCGGCGAAG
TGTGCCGTCACGCGACGCGTCAGACGGACATCGCGGGCCGCATCGGAGGCGAGGAATTTCTGGTCGTGTTTCCGTCGACG
TCGGTCGAGCACGGCGTGTCGGCTGTCGAACGGATGCGCCGCCAGGTGCTGAGCCGCCGCGTGCAGGTCGCCGATGACCG
TGCGATCCGCTATTCCTTTTCGGCGGGCGTCTGCAGGGTGGAACTGAGCGACGACCTCAACACGACGCTCGCTCGCGCCG
ACAGCGCGCTGTATTCGGCGAAGCGCGAAGGACGCAACCGCACCGTCGCGTGGTCGGCACCGTTGCAGTAA

Upstream 100 bases:

>100_bases
TGACCGTGGCGCTTTTCTTGCGAAATCCGGCGAACGGACGGGACATGCGGCCGCGCGCGAGAAAGCGGCCGGTCCGGGGA
CTCATCCGCACCGATAAGCC

Downstream 100 bases:

>100_bases
GTCCGGCCTGAGTTTCCGCGCCTGCACGGCACAACCCGCCAGCAACGCCCCGCAAAACGCGCAATCGCCCCGCGCCGATT
CTCCCCGAAGACCGCCGCCG

Product: diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MKSIHDLRTDDIACEALLGALDGRTQLVAIYDASDRLMFANSAFKHAFRLNRERGDITFADLVLRGILGQCGPRIGGDDA
SAVIADAIAARRERAGQRSFATELVDGRWFWMTESLLENDWIVLTGTEISALRSNEQAWIGAHERAVQEARTDALTGLAN
RRHSMSSLELAITAAAGTETPLTVALVDLDHFKQINDSHGHAAGDAVLRDFGEVCRHATRQTDIAGRIGGEEFLVVFPST
SVEHGVSAVERMRRQVLSRRVQVADDRAIRYSFSAGVCRVELSDDLNTTLARADSALYSAKREGRNRTVAWSAPLQ

Sequences:

>Translated_316_residues
MKSIHDLRTDDIACEALLGALDGRTQLVAIYDASDRLMFANSAFKHAFRLNRERGDITFADLVLRGILGQCGPRIGGDDA
SAVIADAIAARRERAGQRSFATELVDGRWFWMTESLLENDWIVLTGTEISALRSNEQAWIGAHERAVQEARTDALTGLAN
RRHSMSSLELAITAAAGTETPLTVALVDLDHFKQINDSHGHAAGDAVLRDFGEVCRHATRQTDIAGRIGGEEFLVVFPST
SVEHGVSAVERMRRQVLSRRVQVADDRAIRYSFSAGVCRVELSDDLNTTLARADSALYSAKREGRNRTVAWSAPLQ
>Mature_316_residues
MKSIHDLRTDDIACEALLGALDGRTQLVAIYDASDRLMFANSAFKHAFRLNRERGDITFADLVLRGILGQCGPRIGGDDA
SAVIADAIAARRERAGQRSFATELVDGRWFWMTESLLENDWIVLTGTEISALRSNEQAWIGAHERAVQEARTDALTGLAN
RRHSMSSLELAITAAAGTETPLTVALVDLDHFKQINDSHGHAAGDAVLRDFGEVCRHATRQTDIAGRIGGEEFLVVFPST
SVEHGVSAVERMRRQVLSRRVQVADDRAIRYSFSAGVCRVELSDDLNTTLARADSALYSAKREGRNRTVAWSAPLQ

Specific function: Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=168, Percent_Identity=36.9047619047619, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI87082007, Length=164, Percent_Identity=37.8048780487805, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI87081881, Length=167, Percent_Identity=34.1317365269461, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1787262, Length=164, Percent_Identity=33.5365853658537, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1787802, Length=155, Percent_Identity=34.1935483870968, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1786584, Length=170, Percent_Identity=32.3529411764706, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1788381, Length=173, Percent_Identity=33.5260115606936, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1787816, Length=201, Percent_Identity=30.8457711442786, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787541, Length=207, Percent_Identity=29.951690821256, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI87081977, Length=169, Percent_Identity=31.3609467455621, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI87081974, Length=166, Percent_Identity=26.5060240963855, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1788085, Length=164, Percent_Identity=31.7073170731707, Blast_Score=76, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR012292
- InterPro:   IPR009050 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 34660; Mature: 34660

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSIHDLRTDDIACEALLGALDGRTQLVAIYDASDRLMFANSAFKHAFRLNRERGDITFA
CCCCHHCCCHHHHHHHHHHHCCCCEEEEEEEECCCCEEEEHHHHHHHHHHCCCCCCCHHH
DLVLRGILGQCGPRIGGDDASAVIADAIAARRERAGQRSFATELVDGRWFWMTESLLEND
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCEEEEEEHHHHCCC
WIVLTGTEISALRSNEQAWIGAHERAVQEARTDALTGLANRRHSMSSLELAITAAAGTET
EEEEECCHHHHHHCCCCCEECHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCC
PLTVALVDLDHFKQINDSHGHAAGDAVLRDFGEVCRHATRQTDIAGRIGGEEFLVVFPST
CEEEEEECHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCEEEEEECCC
SVEHGVSAVERMRRQVLSRRVQVADDRAIRYSFSAGVCRVELSDDLNTTLARADSALYSA
CHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEEECCCHHHHHHHHHHHHHHH
KREGRNRTVAWSAPLQ
HHCCCCCEEEECCCCC
>Mature Secondary Structure
MKSIHDLRTDDIACEALLGALDGRTQLVAIYDASDRLMFANSAFKHAFRLNRERGDITFA
CCCCHHCCCHHHHHHHHHHHCCCCEEEEEEEECCCCEEEEHHHHHHHHHHCCCCCCCHHH
DLVLRGILGQCGPRIGGDDASAVIADAIAARRERAGQRSFATELVDGRWFWMTESLLEND
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCEEEEEEHHHHCCC
WIVLTGTEISALRSNEQAWIGAHERAVQEARTDALTGLANRRHSMSSLELAITAAAGTET
EEEEECCHHHHHHCCCCCEECHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCC
PLTVALVDLDHFKQINDSHGHAAGDAVLRDFGEVCRHATRQTDIAGRIGGEEFLVVFPST
CEEEEEECHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCEEEEEECCC
SVEHGVSAVERMRRQVLSRRVQVADDRAIRYSFSAGVCRVELSDDLNTTLARADSALYSA
CHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEEECCCHHHHHHHHHHHHHHH
KREGRNRTVAWSAPLQ
HHCCCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]