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The map label for this gene is tuf1 [H]

Identifier: 186477590

GI number: 186477590

Start: 3181527

End: 3182717

Strand: Reverse

Name: tuf1 [H]

Synonym: Bphy_2842

Alternate gene names: 186477590

Gene position: 3182717-3181527 (Counterclockwise)

Preceding gene: 186477591

Following gene: 186477589

Centisome position: 91.48

GC content: 62.22

Gene sequence:

>1191_bases
ATGGCTAAAGGTAAATTCGAACGGACCAAGCCGCACGTGAACGTCGGCACGATCGGTCACGTTGACCACGGCAAGACCAC
GCTGACGGCAGCGATCACGACGGTGCTGACCGCGAAGTTCGGCGGCGAAGCAAAGGCCTACGACCAGATCGACGCGGCAC
CGGAAGAAAAGGCACGCGGCATCACGATCAACACGGCGCACGTCGAGTACGAAACGGCTAACCGCCACTACGCACACGTC
GACTGCCCGGGCCACGCTGACTATGTGAAGAACATGATCACGGGCGCAGCGCAGATGGACGGCGCGATCCTGGTGTGCTC
GGCCGCTGACGGCCCGATGCCGCAAACGCGTGAGCACATCCTGCTGGCGCGTCAGGTTGGCGTTCCGTACATCATCGTGT
TCCTGAACAAGTGCGACATGGTCGACGACGCGGAGCTGCTCGAGCTGGTCGAGATGGAAGTGCGCGAACTGCTGTCGAAG
TACGACTTCCCGGGCGACGACACGCCGATCATCAAGGGTTCGGCGAAGCTGGCGCTGGAAGGCGACAAGGGCGAGCTGGG
CGAAGTGGCGATCATGAATCTGGCCGACGCGCTGGACACGTACATCCCGACGCCGGAGCGCGCAGTTGATGGCGCATTCC
TGATGCCGGTGGAAGACGTGTTCTCGATCTCGGGTCGTGGCACGGTGGTGACGGGCCGCGTCGAGCGCGGCGTCATCAAG
GTCGGCGAAGAAATCGAAATCGTCGGTATCAAGCCGACGGTGAAGACGACGTGCACGGGCGTGGAAATGTTCCGCAAGCT
GCTCGACCAGGGCCAGGCAGGCGACAACGTCGGTATCCTGCTGCGCGGCACGAAGCGTGAAGACGTGGAGCGTGGCCAGG
TTCTGGCGAAGCCGGGTTCGATCACGCCGCACACGCACTTCACGGCTGAAGTGTACGTGCTGAGCAAGGACGAAGGCGGT
CGTCACACGCCGTTCTTCAACAACTACCGTCCGCAGTTCTACTTCCGTACGACGGACGTGACGGGCTCGATCGAGCTGCC
GAAGGACAAGGAAATGGTCATGCCGGGCGACAACGTGTCGATCACGGTGAAGCTGATCGCTCCGATCGCGATGGAAGAAG
GTCTGCGCTTCGCCATCCGCGAAGGTGGCCGTACCGTCGGCGCAGGTGTCGTCGCCAAGATCATCGAGTAA

Upstream 100 bases:

>100_bases
TGAAGCGCCGCGTAACGTGTCGGAAGCGATCATCAACGCGAAGTCGAAGTAAGACCTCGCATAGTCATTCATCGAACAAC
TTTTTTGAAAGAAGAGAAAC

Downstream 100 bases:

>100_bases
AATCGAGGATTGCATCTAGCAGTATGTGCCCGGGTCTGGGCATTCGCCCCTCGCGAGCGGCCCAGACCTACGTTCTTTTA
TTCATAGGCGGTGCAATATC

Product: elongation factor Tu

Products: NA

Alternate protein names: EF-Tu [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MAKGKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLTAKFGGEAKAYDQIDAAPEEKARGITINTAHVEYETANRHYAHV
DCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLARQVGVPYIIVFLNKCDMVDDAELLELVEMEVRELLSK
YDFPGDDTPIIKGSAKLALEGDKGELGEVAIMNLADALDTYIPTPERAVDGAFLMPVEDVFSISGRGTVVTGRVERGVIK
VGEEIEIVGIKPTVKTTCTGVEMFRKLLDQGQAGDNVGILLRGTKREDVERGQVLAKPGSITPHTHFTAEVYVLSKDEGG
RHTPFFNNYRPQFYFRTTDVTGSIELPKDKEMVMPGDNVSITVKLIAPIAMEEGLRFAIREGGRTVGAGVVAKIIE

Sequences:

>Translated_396_residues
MAKGKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLTAKFGGEAKAYDQIDAAPEEKARGITINTAHVEYETANRHYAHV
DCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLARQVGVPYIIVFLNKCDMVDDAELLELVEMEVRELLSK
YDFPGDDTPIIKGSAKLALEGDKGELGEVAIMNLADALDTYIPTPERAVDGAFLMPVEDVFSISGRGTVVTGRVERGVIK
VGEEIEIVGIKPTVKTTCTGVEMFRKLLDQGQAGDNVGILLRGTKREDVERGQVLAKPGSITPHTHFTAEVYVLSKDEGG
RHTPFFNNYRPQFYFRTTDVTGSIELPKDKEMVMPGDNVSITVKLIAPIAMEEGLRFAIREGGRTVGAGVVAKIIE
>Mature_395_residues
AKGKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLTAKFGGEAKAYDQIDAAPEEKARGITINTAHVEYETANRHYAHVD
CPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLARQVGVPYIIVFLNKCDMVDDAELLELVEMEVRELLSKY
DFPGDDTPIIKGSAKLALEGDKGELGEVAIMNLADALDTYIPTPERAVDGAFLMPVEDVFSISGRGTVVTGRVERGVIKV
GEEIEIVGIKPTVKTTCTGVEMFRKLLDQGQAGDNVGILLRGTKREDVERGQVLAKPGSITPHTHFTAEVYVLSKDEGGR
HTPFFNNYRPQFYFRTTDVTGSIELPKDKEMVMPGDNVSITVKLIAPIAMEEGLRFAIREGGRTVGAGVVAKIIE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=394, Percent_Identity=57.1065989847716, Blast_Score=483, Evalue=1e-136,
Organism=Homo sapiens, GI223555963, Length=431, Percent_Identity=30.1624129930394, Blast_Score=155, Evalue=6e-38,
Organism=Homo sapiens, GI5729864, Length=431, Percent_Identity=30.1624129930394, Blast_Score=155, Evalue=7e-38,
Organism=Homo sapiens, GI4503475, Length=448, Percent_Identity=28.125, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI4503471, Length=448, Percent_Identity=28.5714285714286, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI194018522, Length=434, Percent_Identity=28.3410138248848, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI46094014, Length=439, Percent_Identity=27.5626423690205, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI194018520, Length=434, Percent_Identity=28.3410138248848, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI194097354, Length=434, Percent_Identity=28.3410138248848, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI54607086, Length=331, Percent_Identity=29.9093655589124, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI4503507, Length=268, Percent_Identity=28.7313432835821, Blast_Score=92, Evalue=9e-19,
Organism=Homo sapiens, GI94966754, Length=135, Percent_Identity=31.1111111111111, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI157426893, Length=285, Percent_Identity=26.3157894736842, Blast_Score=66, Evalue=7e-11,
Organism=Escherichia coli, GI1790412, Length=395, Percent_Identity=80.7594936708861, Blast_Score=633, Evalue=0.0,
Organism=Escherichia coli, GI1789737, Length=395, Percent_Identity=80.7594936708861, Blast_Score=633, Evalue=0.0,
Organism=Escherichia coli, GI2367247, Length=341, Percent_Identity=29.9120234604106, Blast_Score=134, Evalue=7e-33,
Organism=Escherichia coli, GI48994988, Length=274, Percent_Identity=29.9270072992701, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI1789108, Length=318, Percent_Identity=28.6163522012579, Blast_Score=81, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17556456, Length=403, Percent_Identity=52.8535980148883, Blast_Score=412, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI25141371, Length=387, Percent_Identity=44.9612403100775, Blast_Score=335, Evalue=3e-92,
Organism=Caenorhabditis elegans, GI17552884, Length=453, Percent_Identity=30.4635761589404, Blast_Score=155, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI17569207, Length=453, Percent_Identity=30.4635761589404, Blast_Score=155, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI32566303, Length=434, Percent_Identity=28.5714285714286, Blast_Score=130, Evalue=8e-31,
Organism=Caenorhabditis elegans, GI17506081, Length=275, Percent_Identity=33.0909090909091, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI115532067, Length=428, Percent_Identity=26.6355140186916, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI115532065, Length=436, Percent_Identity=26.3761467889908, Blast_Score=122, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI32566629, Length=352, Percent_Identity=28.9772727272727, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI32566301, Length=149, Percent_Identity=36.9127516778523, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17509919, Length=295, Percent_Identity=26.7796610169492, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17557151, Length=237, Percent_Identity=27.4261603375527, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6324761, Length=396, Percent_Identity=66.6666666666667, Blast_Score=538, Evalue=1e-154,
Organism=Saccharomyces cerevisiae, GI6325337, Length=445, Percent_Identity=31.0112359550562, Blast_Score=152, Evalue=7e-38,
Organism=Saccharomyces cerevisiae, GI6319594, Length=445, Percent_Identity=31.0112359550562, Blast_Score=152, Evalue=7e-38,
Organism=Saccharomyces cerevisiae, GI6320377, Length=336, Percent_Identity=30.0595238095238, Blast_Score=93, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6320863, Length=265, Percent_Identity=28.3018867924528, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6322937, Length=358, Percent_Identity=26.2569832402235, Blast_Score=87, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6323098, Length=192, Percent_Identity=29.6875, Blast_Score=64, Evalue=3e-11,
Organism=Drosophila melanogaster, GI281363316, Length=393, Percent_Identity=58.2697201017812, Blast_Score=485, Evalue=1e-137,
Organism=Drosophila melanogaster, GI17864358, Length=393, Percent_Identity=58.2697201017812, Blast_Score=485, Evalue=1e-137,
Organism=Drosophila melanogaster, GI19921738, Length=386, Percent_Identity=51.8134715025907, Blast_Score=410, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24652838, Length=448, Percent_Identity=29.4642857142857, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI17137572, Length=448, Percent_Identity=29.4642857142857, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI45553807, Length=448, Percent_Identity=29.4642857142857, Blast_Score=155, Evalue=5e-38,
Organism=Drosophila melanogaster, GI45553816, Length=448, Percent_Identity=29.4642857142857, Blast_Score=155, Evalue=5e-38,
Organism=Drosophila melanogaster, GI24651721, Length=448, Percent_Identity=29.4642857142857, Blast_Score=155, Evalue=5e-38,
Organism=Drosophila melanogaster, GI17864154, Length=448, Percent_Identity=29.4642857142857, Blast_Score=155, Evalue=5e-38,
Organism=Drosophila melanogaster, GI45550900, Length=436, Percent_Identity=28.2110091743119, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI17137380, Length=338, Percent_Identity=31.6568047337278, Blast_Score=122, Evalue=6e-28,
Organism=Drosophila melanogaster, GI19922690, Length=308, Percent_Identity=30.1948051948052, Blast_Score=115, Evalue=7e-26,
Organism=Drosophila melanogaster, GI24647046, Length=269, Percent_Identity=28.996282527881, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24647048, Length=269, Percent_Identity=28.996282527881, Blast_Score=93, Evalue=3e-19,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: NA

Molecular weight: Translated: 42946; Mature: 42815

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKGKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLTAKFGGEAKAYDQIDAAPEEKARG
CCCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCHHCCC
ITINTAHVEYETANRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHE
LLARQVGVPYIIVFLNKCDMVDDAELLELVEMEVRELLSKYDFPGDDTPIIKGSAKLALE
EEEHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCEEEEEE
GDKGELGEVAIMNLADALDTYIPTPERAVDGAFLMPVEDVFSISGRGTVVTGRVERGVIK
CCCCCCCCEEEHHHHHHHHHHCCCCCHHCCCEEEEEHHHHHCCCCCCEEEECCCCCCEEE
VGEEIEIVGIKPTVKTTCTGVEMFRKLLDQGQAGDNVGILLRGTKREDVERGQVLAKPGS
CCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCEEEECCCC
ITPHTHFTAEVYVLSKDEGGRHTPFFNNYRPQFYFRTTDVTGSIELPKDKEMVMPGDNVS
CCCCCEEEEEEEEEECCCCCCCCCCCCCCCCEEEEEEECCEEEEECCCCCCEEECCCCCE
ITVKLIAPIAMEEGLRFAIREGGRTVGAGVVAKIIE
EEEEEEECHHHHCCCEEEECCCCCEECCHHHHHHCC
>Mature Secondary Structure 
AKGKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLTAKFGGEAKAYDQIDAAPEEKARG
CCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCHHCCC
ITINTAHVEYETANRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHE
LLARQVGVPYIIVFLNKCDMVDDAELLELVEMEVRELLSKYDFPGDDTPIIKGSAKLALE
EEEHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCEEEEEE
GDKGELGEVAIMNLADALDTYIPTPERAVDGAFLMPVEDVFSISGRGTVVTGRVERGVIK
CCCCCCCCEEEHHHHHHHHHHCCCCCHHCCCEEEEEHHHHHCCCCCCEEEECCCCCCEEE
VGEEIEIVGIKPTVKTTCTGVEMFRKLLDQGQAGDNVGILLRGTKREDVERGQVLAKPGS
CCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCEEEECCCC
ITPHTHFTAEVYVLSKDEGGRHTPFFNNYRPQFYFRTTDVTGSIELPKDKEMVMPGDNVS
CCCCCEEEEEEEEEECCCCCCCCCCCCCCCCEEEEEEECCEEEEECCCCCCEEECCCCCE
ITVKLIAPIAMEEGLRFAIREGGRTVGAGVVAKIIE
EEEEEEECHHHHCCCEEEECCCCCEECCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA