Click here to switch to the map view.

The map label for this gene is gltD [H]

Identifier: 186477535

GI number: 186477535

Start: 3134984

End: 3136501

Strand: Reverse

Name: gltD [H]

Synonym: Bphy_2787

Alternate gene names: 186477535

Gene position: 3136501-3134984 (Counterclockwise)

Preceding gene: 186477536

Following gene: 186477534

Centisome position: 90.15

GC content: 63.04

Gene sequence:

>1518_bases
ATGCGGCGGGCGCCGATCCCCTCACCGATTCAGATAGACATAGAGAACCACATGGGCAAGGCAACCGGTTTTCTCGAGTT
CGAGCGCCGTCATGAGGCGTACGAAGCACCCCTCACCCGCGTCAAGCACTACAAGGAATTCGTTTCGGCGCTGACCGACG
ACGAGGCGAAGATCCAGGGCGCGCGTTGCATGGACTGCGGCATCCCATTCTGCAATAACGGCTGCCCCGTCAACAACATC
ATTCCGGACTTCAACGACCTGGTCTTCCGTCAGGACTGGAAGAACGCGATCGAGGTCCTGCACTCGACGAACAATTTCCC
CGAATTCACGGGCCGCATCTGTCCGGCGCCGTGTGAGGCGGCATGTACGCTCGGCATCAACGACGACCCGGTCGGCATCA
AGTCGATCGAACACGCGATCATCGACAAGGCGTGGGCCGAAGGCTGGGTCGCGCCGCAGCCGCCGAAGCACAAGACTGGC
AAGAAGGTTGCCGTGGTCGGTTCCGGCCCCGCGGGCCTGGCCGCTGCGCAGCAACTCGCTCGCGCAGGCCATGACGTGAC
GGTGTTCGAGAAGAACGACCGTATCGGCGGCCTGCTGCGTTACGGCATTCCCGACTTCAAGCTGGAGAAGTGGCTGATCG
ACCGCCGCATGCGCCAGATGGAAGCGGAAGGCGTGACGTTCCGCGCGAACGTGTTCATCGGCAAGGACGCGCTGCCGGCG
CACATCGGCAATACTTCGAAGGAAACCATCACACCGGAAGACCTGAAAGAACAGTTCGACGCCGTGATCCTGACGGGCGG
TTCCGAGACGCCGCGCGATCTGCCCGTGCCGGGCCGTGAACTCTCCGGCATCCATTACGCGATGGAATTCCTGCCGCAGC
AGAACAAGGTCAACGCAGGCGACAAGGTTGCCGATCAACTGCTCGCGAAGGGCAAGCATGTCGTCGTGATTGGCGGCGGC
GACACGGGCTCGGACTGCGTCGGCACGTCGAACCGTCATGGCGCGAAGGGCGTCACGCAGTTCGAACTGCTGCCGCAGCC
GCCTGAAGAGGAAAACAAGCCGCTCGTGTGGCCGTACTGGCCGGTGAAGCTGCGCACGTCGTCGTCGCACGAAGAAGGTT
GCGAGCGCGATTGGGCCGTCGCGACGAAGCGTTTCGAAGGCAAGAACGGCAAGGTCGAGAAGCTGATCGCGGCGCGCGTC
GAATGGAAGGACGGCAAGATGGTCGAAGTGCCGGGCTCCGAGTTCGAAATGAAGGCCGATCTCGTGTTGCTGGCGATGGG
CTTCACTCAGCCGGTTTCGCCCGTGCTGGAAGCGTTCGGCGTCGACAAGGATGCCCGCGGCAACGTGCGCGCCGCGACGG
AAGGCGAGAAGGCCTATTACACGTCGGTCGACAAGGTGTTCACGGCGGGCGATATGCGCCGTGGCCAGTCACTCGTGGTG
TGGGCGATCCGCGAAGGCCGGCAGTGCGCACGTTCCGTCGATGCGTACCTGATGGGTCATTCCGAGCTGCCGCGCTAA

Upstream 100 bases:

>100_bases
TGCGAAGAAGGCTGCCAAGGAAGTGCTCGCGGCCTGATCGTTTCCCGACCAACCCCTGACGACATAACGAAGCGCACCCG
CCGCTTGCGTGCACAACGCG

Downstream 100 bases:

>100_bases
GTCTCGATCGTCCCGGCGGCGCCGCGCTGCATGTGTTGCGCGGCAAGCCGCCCTGTTCTGCAGGAGGTTGGAGACAGACG
CGGGCACCGGCGATTCGACG

Product: glutamate synthase subunit beta

Products: NA

Alternate protein names: NADPH-GOGAT [H]

Number of amino acids: Translated: 505; Mature: 505

Protein sequence:

>505_residues
MRRAPIPSPIQIDIENHMGKATGFLEFERRHEAYEAPLTRVKHYKEFVSALTDDEAKIQGARCMDCGIPFCNNGCPVNNI
IPDFNDLVFRQDWKNAIEVLHSTNNFPEFTGRICPAPCEAACTLGINDDPVGIKSIEHAIIDKAWAEGWVAPQPPKHKTG
KKVAVVGSGPAGLAAAQQLARAGHDVTVFEKNDRIGGLLRYGIPDFKLEKWLIDRRMRQMEAEGVTFRANVFIGKDALPA
HIGNTSKETITPEDLKEQFDAVILTGGSETPRDLPVPGRELSGIHYAMEFLPQQNKVNAGDKVADQLLAKGKHVVVIGGG
DTGSDCVGTSNRHGAKGVTQFELLPQPPEEENKPLVWPYWPVKLRTSSSHEEGCERDWAVATKRFEGKNGKVEKLIAARV
EWKDGKMVEVPGSEFEMKADLVLLAMGFTQPVSPVLEAFGVDKDARGNVRAATEGEKAYYTSVDKVFTAGDMRRGQSLVV
WAIREGRQCARSVDAYLMGHSELPR

Sequences:

>Translated_505_residues
MRRAPIPSPIQIDIENHMGKATGFLEFERRHEAYEAPLTRVKHYKEFVSALTDDEAKIQGARCMDCGIPFCNNGCPVNNI
IPDFNDLVFRQDWKNAIEVLHSTNNFPEFTGRICPAPCEAACTLGINDDPVGIKSIEHAIIDKAWAEGWVAPQPPKHKTG
KKVAVVGSGPAGLAAAQQLARAGHDVTVFEKNDRIGGLLRYGIPDFKLEKWLIDRRMRQMEAEGVTFRANVFIGKDALPA
HIGNTSKETITPEDLKEQFDAVILTGGSETPRDLPVPGRELSGIHYAMEFLPQQNKVNAGDKVADQLLAKGKHVVVIGGG
DTGSDCVGTSNRHGAKGVTQFELLPQPPEEENKPLVWPYWPVKLRTSSSHEEGCERDWAVATKRFEGKNGKVEKLIAARV
EWKDGKMVEVPGSEFEMKADLVLLAMGFTQPVSPVLEAFGVDKDARGNVRAATEGEKAYYTSVDKVFTAGDMRRGQSLVV
WAIREGRQCARSVDAYLMGHSELPR
>Mature_505_residues
MRRAPIPSPIQIDIENHMGKATGFLEFERRHEAYEAPLTRVKHYKEFVSALTDDEAKIQGARCMDCGIPFCNNGCPVNNI
IPDFNDLVFRQDWKNAIEVLHSTNNFPEFTGRICPAPCEAACTLGINDDPVGIKSIEHAIIDKAWAEGWVAPQPPKHKTG
KKVAVVGSGPAGLAAAQQLARAGHDVTVFEKNDRIGGLLRYGIPDFKLEKWLIDRRMRQMEAEGVTFRANVFIGKDALPA
HIGNTSKETITPEDLKEQFDAVILTGGSETPRDLPVPGRELSGIHYAMEFLPQQNKVNAGDKVADQLLAKGKHVVVIGGG
DTGSDCVGTSNRHGAKGVTQFELLPQPPEEENKPLVWPYWPVKLRTSSSHEEGCERDWAVATKRFEGKNGKVEKLIAARV
EWKDGKMVEVPGSEFEMKADLVLLAMGFTQPVSPVLEAFGVDKDARGNVRAATEGEKAYYTSVDKVFTAGDMRRGQSLVV
WAIREGRQCARSVDAYLMGHSELPR

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=494, Percent_Identity=25.5060728744939, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI111118983, Length=166, Percent_Identity=30.1204819277108, Blast_Score=67, Evalue=4e-11,
Organism=Homo sapiens, GI111118981, Length=166, Percent_Identity=30.1204819277108, Blast_Score=67, Evalue=5e-11,
Organism=Escherichia coli, GI1789606, Length=486, Percent_Identity=35.1851851851852, Blast_Score=244, Evalue=8e-66,
Organism=Escherichia coli, GI1788811, Length=467, Percent_Identity=35.7601713062098, Blast_Score=243, Evalue=2e-65,
Organism=Escherichia coli, GI87082180, Length=467, Percent_Identity=33.6188436830835, Blast_Score=218, Evalue=1e-57,
Organism=Escherichia coli, GI1788468, Length=461, Percent_Identity=28.4164859002169, Blast_Score=156, Evalue=3e-39,
Organism=Escherichia coli, GI1789243, Length=438, Percent_Identity=28.0821917808219, Blast_Score=122, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17570289, Length=510, Percent_Identity=45.2941176470588, Blast_Score=429, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI71984108, Length=476, Percent_Identity=24.5798319327731, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17543860, Length=170, Percent_Identity=34.1176470588235, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6320030, Length=502, Percent_Identity=49.203187250996, Blast_Score=473, Evalue=1e-134,
Organism=Drosophila melanogaster, GI28574881, Length=503, Percent_Identity=49.9005964214712, Blast_Score=477, Evalue=1e-135,
Organism=Drosophila melanogaster, GI24665539, Length=503, Percent_Identity=49.9005964214712, Blast_Score=477, Evalue=1e-135,
Organism=Drosophila melanogaster, GI24665547, Length=503, Percent_Identity=49.9005964214712, Blast_Score=475, Evalue=1e-134,
Organism=Drosophila melanogaster, GI24665543, Length=503, Percent_Identity=49.9005964214712, Blast_Score=475, Evalue=1e-134,
Organism=Drosophila melanogaster, GI24640763, Length=487, Percent_Identity=23.8193018480493, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI18858217, Length=487, Percent_Identity=23.8193018480493, Blast_Score=97, Evalue=2e-20,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006005
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 55661; Mature: 55661

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRAPIPSPIQIDIENHMGKATGFLEFERRHEAYEAPLTRVKHYKEFVSALTDDEAKIQG
CCCCCCCCCEEEEEHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCC
ARCMDCGIPFCNNGCPVNNIIPDFNDLVFRQDWKNAIEVLHSTNNFPEFTGRICPAPCEA
CEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCC
ACTLGINDDPVGIKSIEHAIIDKAWAEGWVAPQPPKHKTGKKVAVVGSGPAGLAAAQQLA
EEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHH
RAGHDVTVFEKNDRIGGLLRYGIPDFKLEKWLIDRRMRQMEAEGVTFRANVFIGKDALPA
HCCCCEEEEECCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCCH
HIGNTSKETITPEDLKEQFDAVILTGGSETPRDLPVPGRELSGIHYAMEFLPQQNKVNAG
HCCCCCCCCCCHHHHHHHCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCC
DKVADQLLAKGKHVVVIGGGDTGSDCVGTSNRHGAKGVTQFELLPQPPEEENKPLVWPYW
HHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCEEECCC
PVKLRTSSSHEEGCERDWAVATKRFEGKNGKVEKLIAARVEWKDGKMVEVPGSEFEMKAD
CEEEECCCCCHHHCCHHHHHHHHHCCCCCCCHHHHHHHEEECCCCCEEECCCCCCEEECC
LVLLAMGFTQPVSPVLEAFGVDKDARGNVRAATEGEKAYYTSVDKVFTAGDMRRGQSLVV
EEEEEECCCCCHHHHHHHHCCCCCCCCCEEECCCCCCHHHHHHHHHHCCCCCCCCCEEEE
WAIREGRQCARSVDAYLMGHSELPR
EEEHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MRRAPIPSPIQIDIENHMGKATGFLEFERRHEAYEAPLTRVKHYKEFVSALTDDEAKIQG
CCCCCCCCCEEEEEHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCC
ARCMDCGIPFCNNGCPVNNIIPDFNDLVFRQDWKNAIEVLHSTNNFPEFTGRICPAPCEA
CEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCC
ACTLGINDDPVGIKSIEHAIIDKAWAEGWVAPQPPKHKTGKKVAVVGSGPAGLAAAQQLA
EEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHH
RAGHDVTVFEKNDRIGGLLRYGIPDFKLEKWLIDRRMRQMEAEGVTFRANVFIGKDALPA
HCCCCEEEEECCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCCH
HIGNTSKETITPEDLKEQFDAVILTGGSETPRDLPVPGRELSGIHYAMEFLPQQNKVNAG
HCCCCCCCCCCHHHHHHHCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCC
DKVADQLLAKGKHVVVIGGGDTGSDCVGTSNRHGAKGVTQFELLPQPPEEENKPLVWPYW
HHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCEEECCC
PVKLRTSSSHEEGCERDWAVATKRFEGKNGKVEKLIAARVEWKDGKMVEVPGSEFEMKAD
CEEEECCCCCHHHCCHHHHHHHHHCCCCCCCHHHHHHHEEECCCCCEEECCCCCCEEECC
LVLLAMGFTQPVSPVLEAFGVDKDARGNVRAATEGEKAYYTSVDKVFTAGDMRRGQSLVV
EEEEEECCCCCHHHHHHHHCCCCCCCCCEEECCCCCCHHHHHHHHHHCCCCCCCCCEEEE
WAIREGRQCARSVDAYLMGHSELPR
EEEHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11029411 [H]