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The map label for this gene is mlaE [H]

Identifier: 186477517

GI number: 186477517

Start: 3118366

End: 3119133

Strand: Reverse

Name: mlaE [H]

Synonym: Bphy_2769

Alternate gene names: 186477517

Gene position: 3119133-3118366 (Counterclockwise)

Preceding gene: 186477518

Following gene: 186477516

Centisome position: 89.65

GC content: 63.67

Gene sequence:

>768_bases
ATGATCAGCACCATCGGTCGTTCCGTCATCTGCGGGCTCGGGCAGACGGGGTATGCGGCGCGCATGTTCTTGCGCCTCGT
GCTGGAATTTTTTCCTCTGCTGCGCCGTCCGCGTCTCGTTACGAAGCAGATCCACTTCGTCGGTAACTATTCGCTGGTGA
TCATCGCGGTGTCGGGGCTGTTCGTCGGCTTCGTGCTCGGACTGCAAGGCTATTACACGCTCAACCGCTACGGCTCCGAA
CAGGCGCTGGGCTTGCTGGTCGCGCTGTCGCTGGTGCGCGAACTCGGGCCCGTGGTGACGGCGCTGCTGTTCGCCGGCCG
CGCGGGCACGTCGCTCACGGCCGAGATCGGCCTGATGAAGGCGGGCGAACAACTCACGGCAATGGAAATGATGGCCGTCG
ACCCGTTGAAGGTGGTCGTCGCACCGCGCATGTGGGCGGGCATCATCGCGATGCCGGTTCTCGCCGCCATCTTCAGCGCA
GTGGGCGTGATCGGCGGCTATATCGTGGGCGTCCTGATGATCGGCGTCGACGCTGGTGCGTTCTGGTCGCAGATGCAGGG
CGGTGTCGATGCTTATCGTGACGTCGGCAACGGCGTGATCAAAAGCATCGTGTTCGGCTTCGCGGTGACGTTCATCGCGC
TCTACCAGGGCTACGAAGCGAAGCCCACGCCGGAAGGCGTGTCGCGCGCGACGACGAAGACGGTCGTCTACGCATCGCTC
GCCGTGCTCGGTCTGGATTTCCTGCTGACCGCGCTGATGTTCAGCTAG

Upstream 100 bases:

>100_bases
TGCGCCAGTTCATCGACGGCACGCCCGATGGCCCGTTCAAATTTCACTATCCGAGCAACACGTCGCTGGCGGCGGACTTC
GGCATCGGCGGAGGCCGCGC

Downstream 100 bases:

>100_bases
GCGGCGCCGCACCGAACCCTGCGCGCCGCGCCGCGCAGGCAGTGCGCCGCTGCGGCATAGATTCTCTTTGGGATGACGAT
GAAAAAAACTGCTCTCGATT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MISTIGRSVICGLGQTGYAARMFLRLVLEFFPLLRRPRLVTKQIHFVGNYSLVIIAVSGLFVGFVLGLQGYYTLNRYGSE
QALGLLVALSLVRELGPVVTALLFAGRAGTSLTAEIGLMKAGEQLTAMEMMAVDPLKVVVAPRMWAGIIAMPVLAAIFSA
VGVIGGYIVGVLMIGVDAGAFWSQMQGGVDAYRDVGNGVIKSIVFGFAVTFIALYQGYEAKPTPEGVSRATTKTVVYASL
AVLGLDFLLTALMFS

Sequences:

>Translated_255_residues
MISTIGRSVICGLGQTGYAARMFLRLVLEFFPLLRRPRLVTKQIHFVGNYSLVIIAVSGLFVGFVLGLQGYYTLNRYGSE
QALGLLVALSLVRELGPVVTALLFAGRAGTSLTAEIGLMKAGEQLTAMEMMAVDPLKVVVAPRMWAGIIAMPVLAAIFSA
VGVIGGYIVGVLMIGVDAGAFWSQMQGGVDAYRDVGNGVIKSIVFGFAVTFIALYQGYEAKPTPEGVSRATTKTVVYASL
AVLGLDFLLTALMFS
>Mature_255_residues
MISTIGRSVICGLGQTGYAARMFLRLVLEFFPLLRRPRLVTKQIHFVGNYSLVIIAVSGLFVGFVLGLQGYYTLNRYGSE
QALGLLVALSLVRELGPVVTALLFAGRAGTSLTAEIGLMKAGEQLTAMEMMAVDPLKVVVAPRMWAGIIAMPVLAAIFSA
VGVIGGYIVGVLMIGVDAGAFWSQMQGGVDAYRDVGNGVIKSIVFGFAVTFIALYQGYEAKPTPEGVSRATTKTVVYASL
AVLGLDFLLTALMFS

Specific function: Part of the ABC transporter complex mlaFEDB that actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner memb

COG id: COG0767

COG function: function code Q; ABC-type transport system involved in resistance to organic solvents, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaE permease family [H]

Homologues:

Organism=Escherichia coli, GI1789585, Length=253, Percent_Identity=56.1264822134387, Blast_Score=276, Evalue=1e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003453 [H]

Pfam domain/function: PF02405 DUF140 [H]

EC number: NA

Molecular weight: Translated: 27087; Mature: 27087

Theoretical pI: Translated: 9.67; Mature: 9.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHCC
>Mature Secondary Structure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HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]