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The map label for this gene is engD [H]

Identifier: 186477456

GI number: 186477456

Start: 3062226

End: 3063320

Strand: Direct

Name: engD [H]

Synonym: Bphy_2708

Alternate gene names: 186477456

Gene position: 3062226-3063320 (Clockwise)

Preceding gene: 186477451

Following gene: 186477457

Centisome position: 88.02

GC content: 61.92

Gene sequence:

>1095_bases
ATGAGCCTCAAATGCGGCATCGTCGGCCTGCCTAACGTCGGCAAGTCCACCCTGTTCAACGCGCTGACCAAGGCGGGCAT
CGCCGCCGAAAACTATCCCTTCTGCACGATCGAGCCGAACGTTGGCGTGGTCGAAGTGCCGGACGCGCGTCTGAAGGCGC
TATCCGAAATCGTCAAGCCGGAGCGCATCGTCCCCGCCGTCGTCGAGTTCGTCGACATTGCGGGCCTCGTTGCAGGCGCG
AGCAAGGGTGAGGGCCTCGGCAACCAGTTTCTCGCGAACATCCGCGAAACCGATGCGATCACGCACGTCGTCCGCTGCTT
CGAAGACGAGAACGTGATTCACGTCGCGGGCAAGGTGGACCCTGTGTCGGACATCGAGGTCATCAACACGGAACTGGCGC
TCGCCGACCTCGCGACGGTCGAAAAGGCCCTCACGCGTTATGCCAAAGCCGCCAAGTCCGGCAACGACAAGGAAGCGGTG
AAGCTCGCAGCCGTGCTGGAGAAGGTTCGCGTGCAACTGGACCAGGCGAAGCCGGTGCGCGGCCTCGATCTGACGGAAGA
AGAGCGTTTGCTGATCAAGCCGTTCTGCCTGATTACGGCCAAGCCGGCCATGTACGTCGCGAACGTCAAGGAAGACGGCT
TCGAGAACAATCCGCATCTGGACGCGGTGCGCAACTATGCGGAATCCGAGAAAGCGCCCGTGGTTGCAGTATGCGCGGCG
ATCGAGGCGGAAATCGCCGATCTCGCTGATGAAGACAAGGAAGTGTTTCTTGCCGACATGGGCATGGACGAGCCGGGCCT
GAACCGCGTGATCCGCGCCGGCTTCAAGCTGCTCGGGCTGCAGACGTACTTCACGGCGGGCGTGAAGGAAGTGCGCGCGT
GGACGATCCACATCGGCGATACCGCGCCGCAGGCGGCGGGCGCGATTCATACCGACTTCGAGCGCGGCTTCATTCGCGCG
CAGACGATCGGCTTCGACGATTACATCGCGTACAAGGGCGAGCAAGGCGCGAAGGAAGCGGGCAAGATGCGCGCCGAAGG
CAAGGAGTACATCGTGCATGACGGCGACGTGATGAATTTTCTCTTCAACGTTTGA

Upstream 100 bases:

>100_bases
GCGGGCCGTTGCCGGCATCGCGCCGCGCCGCGTGCAACCCGTACGGCAAGTCCGCTCCGTTCGCCGAAGCCTGTCACCAC
CTGATTTGTAGAGAACCTTC

Downstream 100 bases:

>100_bases
CCAGCAATTGTTAATTCATCCCCGCCGCCTCGAGCGGGGTTCGCACAGCACATCAGGCCGCGCTACGACGCGGCTTTTTT
TTCGCCTTCGTTGCCCGCGC

Product: GTP-dependent nucleic acid-binding protein EngD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 364; Mature: 363

Protein sequence:

>364_residues
MSLKCGIVGLPNVGKSTLFNALTKAGIAAENYPFCTIEPNVGVVEVPDARLKALSEIVKPERIVPAVVEFVDIAGLVAGA
SKGEGLGNQFLANIRETDAITHVVRCFEDENVIHVAGKVDPVSDIEVINTELALADLATVEKALTRYAKAAKSGNDKEAV
KLAAVLEKVRVQLDQAKPVRGLDLTEEERLLIKPFCLITAKPAMYVANVKEDGFENNPHLDAVRNYAESEKAPVVAVCAA
IEAEIADLADEDKEVFLADMGMDEPGLNRVIRAGFKLLGLQTYFTAGVKEVRAWTIHIGDTAPQAAGAIHTDFERGFIRA
QTIGFDDYIAYKGEQGAKEAGKMRAEGKEYIVHDGDVMNFLFNV

Sequences:

>Translated_364_residues
MSLKCGIVGLPNVGKSTLFNALTKAGIAAENYPFCTIEPNVGVVEVPDARLKALSEIVKPERIVPAVVEFVDIAGLVAGA
SKGEGLGNQFLANIRETDAITHVVRCFEDENVIHVAGKVDPVSDIEVINTELALADLATVEKALTRYAKAAKSGNDKEAV
KLAAVLEKVRVQLDQAKPVRGLDLTEEERLLIKPFCLITAKPAMYVANVKEDGFENNPHLDAVRNYAESEKAPVVAVCAA
IEAEIADLADEDKEVFLADMGMDEPGLNRVIRAGFKLLGLQTYFTAGVKEVRAWTIHIGDTAPQAAGAIHTDFERGFIRA
QTIGFDDYIAYKGEQGAKEAGKMRAEGKEYIVHDGDVMNFLFNV
>Mature_363_residues
SLKCGIVGLPNVGKSTLFNALTKAGIAAENYPFCTIEPNVGVVEVPDARLKALSEIVKPERIVPAVVEFVDIAGLVAGAS
KGEGLGNQFLANIRETDAITHVVRCFEDENVIHVAGKVDPVSDIEVINTELALADLATVEKALTRYAKAAKSGNDKEAVK
LAAVLEKVRVQLDQAKPVRGLDLTEEERLLIKPFCLITAKPAMYVANVKEDGFENNPHLDAVRNYAESEKAPVVAVCAAI
EAEIADLADEDKEVFLADMGMDEPGLNRVIRAGFKLLGLQTYFTAGVKEVRAWTIHIGDTAPQAAGAIHTDFERGFIRAQ
TIGFDDYIAYKGEQGAKEAGKMRAEGKEYIVHDGDVMNFLFNV

Specific function: GTP-dependent nucleic acid-binding protein which may act as a translation factor [H]

COG id: COG0012

COG function: function code J; Predicted GTPase, probable translation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI58761500, Length=369, Percent_Identity=43.360433604336, Blast_Score=303, Evalue=2e-82,
Organism=Homo sapiens, GI58761502, Length=227, Percent_Identity=36.5638766519824, Blast_Score=146, Evalue=3e-35,
Organism=Escherichia coli, GI1787454, Length=364, Percent_Identity=71.1538461538462, Blast_Score=530, Evalue=1e-152,
Organism=Escherichia coli, GI1789574, Length=141, Percent_Identity=31.9148936170213, Blast_Score=65, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17509631, Length=366, Percent_Identity=42.0765027322404, Blast_Score=293, Evalue=1e-79,
Organism=Saccharomyces cerevisiae, GI6319499, Length=369, Percent_Identity=40.650406504065, Blast_Score=251, Evalue=1e-67,
Organism=Saccharomyces cerevisiae, GI6321773, Length=382, Percent_Identity=34.5549738219895, Blast_Score=195, Evalue=1e-50,
Organism=Saccharomyces cerevisiae, GI6321649, Length=166, Percent_Identity=38.5542168674699, Blast_Score=89, Evalue=9e-19,
Organism=Drosophila melanogaster, GI24640873, Length=367, Percent_Identity=41.1444141689373, Blast_Score=286, Evalue=2e-77,
Organism=Drosophila melanogaster, GI24640877, Length=367, Percent_Identity=41.1444141689373, Blast_Score=286, Evalue=2e-77,
Organism=Drosophila melanogaster, GI24640875, Length=367, Percent_Identity=41.1444141689373, Blast_Score=286, Evalue=2e-77,
Organism=Drosophila melanogaster, GI24640879, Length=325, Percent_Identity=38.4615384615385, Blast_Score=227, Evalue=8e-60,
Organism=Drosophila melanogaster, GI24585318, Length=137, Percent_Identity=31.3868613138686, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR004396
- InterPro:   IPR013029
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR012676
- InterPro:   IPR023192 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF06071 YchF-GTPase_C [H]

EC number: NA

Molecular weight: Translated: 39339; Mature: 39208

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLKCGIVGLPNVGKSTLFNALTKAGIAAENYPFCTIEPNVGVVEVPDARLKALSEIVKP
CCCEECEEECCCCCHHHHHHHHHHHCCCCCCCCEEEECCCCCEEECCHHHHHHHHHHHCC
ERIVPAVVEFVDIAGLVAGASKGEGLGNQFLANIRETDAITHVVRCFEDENVIHVAGKVD
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCC
PVSDIEVINTELALADLATVEKALTRYAKAAKSGNDKEAVKLAAVLEKVRVQLDQAKPVR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
GLDLTEEERLLIKPFCLITAKPAMYVANVKEDGFENNPHLDAVRNYAESEKAPVVAVCAA
CCCCCCCCCEEEEEEEEEEECCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCCEEEHHHH
IEAEIADLADEDKEVFLADMGMDEPGLNRVIRAGFKLLGLQTYFTAGVKEVRAWTIHIGD
HHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEECC
TAPQAAGAIHTDFERGFIRAQTIGFDDYIAYKGEQGAKEAGKMRAEGKEYIVHDGDVMNF
CCCHHCCCHHCHHHHCEEEEEECCCCHHEEECCCCCHHHHHHHHCCCCEEEEECCHHHHH
LFNV
HHCC
>Mature Secondary Structure 
SLKCGIVGLPNVGKSTLFNALTKAGIAAENYPFCTIEPNVGVVEVPDARLKALSEIVKP
CCEECEEECCCCCHHHHHHHHHHHCCCCCCCCEEEECCCCCEEECCHHHHHHHHHHHCC
ERIVPAVVEFVDIAGLVAGASKGEGLGNQFLANIRETDAITHVVRCFEDENVIHVAGKVD
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCC
PVSDIEVINTELALADLATVEKALTRYAKAAKSGNDKEAVKLAAVLEKVRVQLDQAKPVR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
GLDLTEEERLLIKPFCLITAKPAMYVANVKEDGFENNPHLDAVRNYAESEKAPVVAVCAA
CCCCCCCCCEEEEEEEEEEECCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCCEEEHHHH
IEAEIADLADEDKEVFLADMGMDEPGLNRVIRAGFKLLGLQTYFTAGVKEVRAWTIHIGD
HHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEECC
TAPQAAGAIHTDFERGFIRAQTIGFDDYIAYKGEQGAKEAGKMRAEGKEYIVHDGDVMNF
CCCHHCCCHHCHHHHCEEEEEECCCCHHEEECCCCCHHHHHHHHCCCCEEEEECCHHHHH
LFNV
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]