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The map label for this gene is mraW

Identifier: 186477429

GI number: 186477429

Start: 3025417

End: 3026370

Strand: Reverse

Name: mraW

Synonym: Bphy_2681

Alternate gene names: 186477429

Gene position: 3026370-3025417 (Counterclockwise)

Preceding gene: 186477430

Following gene: 186477428

Centisome position: 86.98

GC content: 63.0

Gene sequence:

>954_bases
ATGGCGCCTGCGATGGGAAACGAATTGCAGCATCGCACGGTGCTGCTGGAAGAAGCGGTGAACGCGCTCGTCACGCGCGT
GGACGGTATCTATATAGATGGCACGTTCGGGCGCGGCGGTCACAGCCGCGCGGTATTGGCAAAGCTGGGTGAAGCAGGTC
GTCTGATTGCGTTCGACAAAGACCCGCTCGCCATTGCCACCGCGCAGCAGGTCGCCGACCCGCGCTTCGAGATCGTGCAT
GAAAGCTTTGCATCACTGCGCGACGCGATGAGCGAGCGCGCGGTAGGGCGTGTGTCGGGTGTGCTGCTGGATCTGGGCGT
GTCGTCGCCGCAGTTCGACGACCCGGAGCGGGGTTTCAGTTTCCGCGCCGACGGCCCGCTCGACATGCGGATGGACCCGA
CGCGCGGCGAATCCGCTGCGGACTGGCTGGCGCGGGCGACGGTGCAGGAAATGACGGAGGTGATACGGGATTATGGGGAA
GAACGGTTTGCTTTTCAGATTGCAAAGGCGCTTGTTGCTCGCCGGGCAGAGTCCGACCGTCTTGGGCCTCTCGTCAGCAC
GGGCGAGCTTGCCCAAATCGTGGCTAACGTCGTCAAAACCCGTGAGAAGGGTAAGGACCCGGCAACCCGCACCTTTCAGG
CTATACGGATTCACATCAATCAAGAGCTTGCGGAGCTGCAAGTCGTACTAGAAGCAGCATTGTCGTTGCTGGAGCAAGGG
GGGCGGCTGGTCGTGATCAGCTTTCATTCGCTCGAAGACCGGATCGTCAAACGGTTCATGCAGACGCACTCGAGTGCGCC
TGCTGTCGATCGCCGTCTGCCGATCCGCGCCGTTGACTTGCCGAGCCCGCCGCTCAAACTGCTGGGCCGCGTATTCGCGA
GCGACGAAGAAGTCGCCGCGAACCCCCGCGCCCGTTCCGCCGTGATGCGCGTTGCGGAGCGGATCGCGCCATGA

Upstream 100 bases:

>100_bases
CTGGGACAAGGAAACCTACGACGCGCAGGAACAGGAAGCGATGTCGCAAGGCATGCCCGAGTCCTTGAAAAACTTCACAT
TCTGACCGCAGGTAATACAT

Downstream 100 bases:

>100_bases
ACCGTCTCAACATCTTTCTGCTGATCGTCGTCATGGGCTGCGCGCTTTCCGTCGTCAACGCGACCAATCAGCAGCGCCAG
TTCTTCATCCAGTTGCAGCG

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 317; Mature: 316

Protein sequence:

>317_residues
MAPAMGNELQHRTVLLEEAVNALVTRVDGIYIDGTFGRGGHSRAVLAKLGEAGRLIAFDKDPLAIATAQQVADPRFEIVH
ESFASLRDAMSERAVGRVSGVLLDLGVSSPQFDDPERGFSFRADGPLDMRMDPTRGESAADWLARATVQEMTEVIRDYGE
ERFAFQIAKALVARRAESDRLGPLVSTGELAQIVANVVKTREKGKDPATRTFQAIRIHINQELAELQVVLEAALSLLEQG
GRLVVISFHSLEDRIVKRFMQTHSSAPAVDRRLPIRAVDLPSPPLKLLGRVFASDEEVAANPRARSAVMRVAERIAP

Sequences:

>Translated_317_residues
MAPAMGNELQHRTVLLEEAVNALVTRVDGIYIDGTFGRGGHSRAVLAKLGEAGRLIAFDKDPLAIATAQQVADPRFEIVH
ESFASLRDAMSERAVGRVSGVLLDLGVSSPQFDDPERGFSFRADGPLDMRMDPTRGESAADWLARATVQEMTEVIRDYGE
ERFAFQIAKALVARRAESDRLGPLVSTGELAQIVANVVKTREKGKDPATRTFQAIRIHINQELAELQVVLEAALSLLEQG
GRLVVISFHSLEDRIVKRFMQTHSSAPAVDRRLPIRAVDLPSPPLKLLGRVFASDEEVAANPRARSAVMRVAERIAP
>Mature_316_residues
APAMGNELQHRTVLLEEAVNALVTRVDGIYIDGTFGRGGHSRAVLAKLGEAGRLIAFDKDPLAIATAQQVADPRFEIVHE
SFASLRDAMSERAVGRVSGVLLDLGVSSPQFDDPERGFSFRADGPLDMRMDPTRGESAADWLARATVQEMTEVIRDYGEE
RFAFQIAKALVARRAESDRLGPLVSTGELAQIVANVVKTREKGKDPATRTFQAIRIHINQELAELQVVLEAALSLLEQGG
RLVVISFHSLEDRIVKRFMQTHSSAPAVDRRLPIRAVDLPSPPLKLLGRVFASDEEVAANPRARSAVMRVAERIAP

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=341, Percent_Identity=37.5366568914956, Blast_Score=183, Evalue=2e-46,
Organism=Homo sapiens, GI165377202, Length=198, Percent_Identity=38.8888888888889, Blast_Score=122, Evalue=6e-28,
Organism=Escherichia coli, GI1786270, Length=314, Percent_Identity=55.7324840764331, Blast_Score=343, Evalue=7e-96,
Organism=Drosophila melanogaster, GI62472493, Length=331, Percent_Identity=38.6706948640483, Blast_Score=173, Evalue=2e-43,
Organism=Drosophila melanogaster, GI28571637, Length=331, Percent_Identity=38.6706948640483, Blast_Score=173, Evalue=2e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_BURP8 (B2JHG8)

Other databases:

- EMBL:   CP001043
- RefSeq:   YP_001858899.1
- ProteinModelPortal:   B2JHG8
- SMR:   B2JHG8
- GeneID:   6244164
- GenomeReviews:   CP001043_GR
- KEGG:   bph:Bphy_2681
- HOGENOM:   HBG302779
- OMA:   PQLDDPE
- ProtClustDB:   PRK00050
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 34690; Mature: 34559

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: NA

Important sites: BINDING 59-59 BINDING 83-83 BINDING 104-104 BINDING 111-111

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPAMGNELQHRTVLLEEAVNALVTRVDGIYIDGTFGRGGHSRAVLAKLGEAGRLIAFDK
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCHHHHHHHHCCCCCEEEECC
DPLAIATAQQVADPRFEIVHESFASLRDAMSERAVGRVSGVLLDLGVSSPQFDDPERGFS
CCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCC
FRADGPLDMRMDPTRGESAADWLARATVQEMTEVIRDYGEERFAFQIAKALVARRAESDR
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCC
LGPLVSTGELAQIVANVVKTREKGKDPATRTFQAIRIHINQELAELQVVLEAALSLLEQG
CCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GRLVVISFHSLEDRIVKRFMQTHSSAPAVDRRLPIRAVDLPSPPLKLLGRVFASDEEVAA
CCEEEEEHHHHHHHHHHHHHHHCCCCCCHHCCCCEEEECCCCCHHHHHHHHHCCCCHHHC
NPRARSAVMRVAERIAP
CCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
APAMGNELQHRTVLLEEAVNALVTRVDGIYIDGTFGRGGHSRAVLAKLGEAGRLIAFDK
CCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCHHHHHHHHCCCCCEEEECC
DPLAIATAQQVADPRFEIVHESFASLRDAMSERAVGRVSGVLLDLGVSSPQFDDPERGFS
CCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCC
FRADGPLDMRMDPTRGESAADWLARATVQEMTEVIRDYGEERFAFQIAKALVARRAESDR
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCC
LGPLVSTGELAQIVANVVKTREKGKDPATRTFQAIRIHINQELAELQVVLEAALSLLEQG
CCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GRLVVISFHSLEDRIVKRFMQTHSSAPAVDRRLPIRAVDLPSPPLKLLGRVFASDEEVAA
CCEEEEEHHHHHHHHHHHHHHHCCCCCCHHCCCCEEEECCCCCHHHHHHHHHCCCCHHHC
NPRARSAVMRVAERIAP
CCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA