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The map label for this gene is ampD [H]

Identifier: 186477388

GI number: 186477388

Start: 2981495

End: 2982133

Strand: Reverse

Name: ampD [H]

Synonym: Bphy_2640

Alternate gene names: 186477388

Gene position: 2982133-2981495 (Counterclockwise)

Preceding gene: 186477389

Following gene: 186477387

Centisome position: 85.71

GC content: 66.04

Gene sequence:

>639_bases
ATGACGGCAGGAGGGCAGTCGCCAGCGCCCGCGCCGGCGCGCTTCACCGTCGACGCAAATGGCTGGGTCGACGAAGCACG
CCAGCTGCCGTCGCCGAATTTCGAGGCGCGCCCGAACGGCGCACGTCCGACGCTGATCGTCGTCCACAACATCAGCTTGC
CGCCGAACGAATTCGGCGGTCCGGGCATCACCGATCTCTTCCTCAACCGCCTCGACTGCGACGCGCATCCGTACTACGAC
GCGCATTTGCGCGACGTGCGCGTGTCTGCGCATTTCGTGATCCGCCGCGATGGCGCGCTCGAACAGTACGTCTCCTGCGA
CGAGCGCGCGTGGCATGCGGGCGCGTCGAACTTCTTCGGCCGCGAGCGCTGCAACGACTTCTCGATCGGCATTGAACTGG
AAGGCAGCGACGCATCGCCGTTCGAACGCGCGCAGTACGCGACGCTAGCACCGCTCGTGCGGTCGCTTGCCGCACACTAT
GAGATCGACTCGCTCGCCGGTCACTCGGACATCGCGCCAGGCCGCAAGACCGACCCGGGCCCGCATTTCGAATGGCCGCG
TCTTCAGCGCGAGACCGCGCTCCCCGATCAGTACTTCCCCTATCTGCATCCGTCTTCGCGCAAGCCGATAAGGTCGTAG

Upstream 100 bases:

>100_bases
ATGCACCGAAGAGCGACTCCGTGCTCGTCGCGGGGCAGACGTTCTGCTCGCACGATCACGCGAAGCGTTACGCCGCCCGA
CCGACGGGCCGCGACGCACG

Downstream 100 bases:

>100_bases
GCGCGTCACTCCTGTTTGTACGGTGCCGCACCGACAACGCAAAAGCGTTGTATGCCCGCCGGCGTCTTCTGACGCGGGCT
ATCCAAAAGTCAAGATGATC

Product: N-acetyl-anhydromuranmyl-L-alanine amidase

Products: NA

Alternate protein names: N-acetylmuramoyl-L-alanine amidase [H]

Number of amino acids: Translated: 212; Mature: 211

Protein sequence:

>212_residues
MTAGGQSPAPAPARFTVDANGWVDEARQLPSPNFEARPNGARPTLIVVHNISLPPNEFGGPGITDLFLNRLDCDAHPYYD
AHLRDVRVSAHFVIRRDGALEQYVSCDERAWHAGASNFFGRERCNDFSIGIELEGSDASPFERAQYATLAPLVRSLAAHY
EIDSLAGHSDIAPGRKTDPGPHFEWPRLQRETALPDQYFPYLHPSSRKPIRS

Sequences:

>Translated_212_residues
MTAGGQSPAPAPARFTVDANGWVDEARQLPSPNFEARPNGARPTLIVVHNISLPPNEFGGPGITDLFLNRLDCDAHPYYD
AHLRDVRVSAHFVIRRDGALEQYVSCDERAWHAGASNFFGRERCNDFSIGIELEGSDASPFERAQYATLAPLVRSLAAHY
EIDSLAGHSDIAPGRKTDPGPHFEWPRLQRETALPDQYFPYLHPSSRKPIRS
>Mature_211_residues
TAGGQSPAPAPARFTVDANGWVDEARQLPSPNFEARPNGARPTLIVVHNISLPPNEFGGPGITDLFLNRLDCDAHPYYDA
HLRDVRVSAHFVIRRDGALEQYVSCDERAWHAGASNFFGRERCNDFSIGIELEGSDASPFERAQYATLAPLVRSLAAHYE
IDSLAGHSDIAPGRKTDPGPHFEWPRLQRETALPDQYFPYLHPSSRKPIRS

Specific function: Involved in both cell wall peptidoglycans recycling and beta-lactamase induction. Specifically cleaves the amide bond between the lactyl group of N-acetylmuramic acid and the alpha- amino group of the L-alanine in degradation products containing an anhydr

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1786300, Length=172, Percent_Identity=55.8139534883721, Blast_Score=194, Evalue=4e-51,
Organism=Escherichia coli, GI1787092, Length=149, Percent_Identity=38.9261744966443, Blast_Score=85, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 23530; Mature: 23399

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAGGQSPAPAPARFTVDANGWVDEARQLPSPNFEARPNGARPTLIVVHNISLPPNEFGG
CCCCCCCCCCCCEEEEEECCCCHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCC
PGITDLFLNRLDCDAHPYYDAHLRDVRVSAHFVIRRDGALEQYVSCDERAWHAGASNFFG
CCHHHHHHHHHCCCCCCCCCCHHEEEEEEEEEEEECCCCHHHHHCHHHHHHHCCCHHCCC
RERCNDFSIGIELEGSDASPFERAQYATLAPLVRSLAAHYEIDSLAGHSDIAPGRKTDPG
HHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCC
PHFEWPRLQRETALPDQYFPYLHPSSRKPIRS
CCCCCCCCHHHHCCCHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
TAGGQSPAPAPARFTVDANGWVDEARQLPSPNFEARPNGARPTLIVVHNISLPPNEFGG
CCCCCCCCCCCEEEEEECCCCHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCC
PGITDLFLNRLDCDAHPYYDAHLRDVRVSAHFVIRRDGALEQYVSCDERAWHAGASNFFG
CCHHHHHHHHHCCCCCCCCCCHHEEEEEEEEEEEECCCCHHHHHCHHHHHHHCCCHHCCC
RERCNDFSIGIELEGSDASPFERAQYATLAPLVRSLAAHYEIDSLAGHSDIAPGRKTDPG
HHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCC
PHFEWPRLQRETALPDQYFPYLHPSSRKPIRS
CCCCCCCCHHHHCCCHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609; 8419294 [H]