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The map label for this gene is mpl [H]

Identifier: 186477373

GI number: 186477373

Start: 2964405

End: 2965814

Strand: Reverse

Name: mpl [H]

Synonym: Bphy_2625

Alternate gene names: 186477373

Gene position: 2965814-2964405 (Counterclockwise)

Preceding gene: 186477384

Following gene: 186477372

Centisome position: 85.24

GC content: 65.67

Gene sequence:

>1410_bases
ATGCACATTCACATCCTCGGCATCTGCGGCACATTCATGGGTGGTCTCGCGGTTCTCGCGCGCAACGCCGGTCACACGGT
GACGGGCTGCGACGCCGGCGTCTATCCGCCGATGAGCACGCAGCTCGAGGCGCAAGGCATCAAGCTGATCGAAGGCTGGG
GCGTCGAGCAACTCGATCTGAAGCCGGACCTGTTCGTGGTCGGCAACGTCGTGACGCGCGGCAATCCGCTGATGGAAGAG
ATCCTCAATCGAGGACTGCCGTACACGTCCGGCCCGCAGTGGCTCGGCGAGCATGTGCTTAACGGCAAATGGGTGCTGGC
CGTGGCAGGCACGCACGGCAAGACCACGACGACGTCGATGCTGACGTGGCTGCTCGAAGACGCGGGCATGAACCCAGGCT
TTCTGATCGGCGGCGTGCCGCTGAACTTCGGTGTGTCGGCGCGGCTGACGGATTCCAGCTTTTTCGTGATCGAAGCCGAC
GAATACGACACCGCGTTCTTCGACAAGCGCTCGAAATTCGTCCACTACCGGCCGCGCACCGCCGTTCTGAACAACCTCGA
ATTCGATCACGCGGATATCTTCCCCGATCTCGCTGCGATCGAAACGCAATTCCACCATCTCGTGCGCACCGTGCCGGGCA
TTGGCCGCGTCGTGTCGAACGGCCGTGAAGTTGCACTGGACCGCGTGCTCGCGCGCGGCTGCTGGAGCGAAGTGGAGCGC
TTCGGCGTCGAGGGCGGCTGGCAGGCGTTGCCCGCTGAAGACGGCGTCGCCGTCGACGAGCGCTTTGCCGTCTATCACAA
CGGCGAGCGCGTCGGTGTGGTCGACTGGCAGGTTCAGGGCGAGCACAACCGGATGAACGCGATCGCCGCGATCGCCGCCG
CGCGCCACATCGGCGTGCCGCCCGCGCAGGCAGCGAAGTCGCTCTCGACGTTCCGCAACGTGAAGCGCCGGATGGAAGTG
CGCGGCAGCGTCGACGGCGTGACCGTCTACGACGATTTCGCGCACCATCCGACGGCGATCCAGACGACGGTCGCGGGTCT
GCGCACGCGTATCGGCCGTACGAATACACGCATTCTCGCCGTGCTGGAGCCGCGCTCGAACACAATGAAGCTCGGCGTGA
TGAAAGCGCAACTCCCGGCGAGCCTGGCCGACGCAGATCTCGTGTTCGGGTACGGCGCACCTTCGGGCAAGGACGCGCTC
GGCTGGAACCTCGCCGAAGCTCTCGCGCCGATGGGCGACAAGGCGCAGGCGTTCAATGAAATCGACTCGCTCGTCAAGGC
AGTCGTCGCGGCCGCGCAGCCGGGCGACCAGGTGCTGGTGATGAGCAACGGCGGTTTCGGCGGCGTGCATCAGAAGCTGC
TCGATGCGCTGTCGGCACGGCCGACGGAGCAGGCGCGGGGCGTCGCGTGA

Upstream 100 bases:

>100_bases
CGGGGCTGTCGGTTACAATACTCGTCCTTTGCACGAGGGCTTTTTCGCCCCGGCGCGCATCTCTTTCCACGCAAAACACG
CCTTGCGGCGCGACAGCTTT

Downstream 100 bases:

>100_bases
TTCTTTATCTGCACGGTTTTCGCTCGTCGCCCAATTCCTTCAAGGCGCGCGTGATGGCCGACCGCCTCGCGGCGCTGGGC
CGCAGCGCCGAGTGGCGCTG

Product: UDP-N-acetylmuramate

Products: NA

Alternate protein names: Murein peptide ligase [H]

Number of amino acids: Translated: 469; Mature: 469

Protein sequence:

>469_residues
MHIHILGICGTFMGGLAVLARNAGHTVTGCDAGVYPPMSTQLEAQGIKLIEGWGVEQLDLKPDLFVVGNVVTRGNPLMEE
ILNRGLPYTSGPQWLGEHVLNGKWVLAVAGTHGKTTTTSMLTWLLEDAGMNPGFLIGGVPLNFGVSARLTDSSFFVIEAD
EYDTAFFDKRSKFVHYRPRTAVLNNLEFDHADIFPDLAAIETQFHHLVRTVPGIGRVVSNGREVALDRVLARGCWSEVER
FGVEGGWQALPAEDGVAVDERFAVYHNGERVGVVDWQVQGEHNRMNAIAAIAAARHIGVPPAQAAKSLSTFRNVKRRMEV
RGSVDGVTVYDDFAHHPTAIQTTVAGLRTRIGRTNTRILAVLEPRSNTMKLGVMKAQLPASLADADLVFGYGAPSGKDAL
GWNLAEALAPMGDKAQAFNEIDSLVKAVVAAAQPGDQVLVMSNGGFGGVHQKLLDALSARPTEQARGVA

Sequences:

>Translated_469_residues
MHIHILGICGTFMGGLAVLARNAGHTVTGCDAGVYPPMSTQLEAQGIKLIEGWGVEQLDLKPDLFVVGNVVTRGNPLMEE
ILNRGLPYTSGPQWLGEHVLNGKWVLAVAGTHGKTTTTSMLTWLLEDAGMNPGFLIGGVPLNFGVSARLTDSSFFVIEAD
EYDTAFFDKRSKFVHYRPRTAVLNNLEFDHADIFPDLAAIETQFHHLVRTVPGIGRVVSNGREVALDRVLARGCWSEVER
FGVEGGWQALPAEDGVAVDERFAVYHNGERVGVVDWQVQGEHNRMNAIAAIAAARHIGVPPAQAAKSLSTFRNVKRRMEV
RGSVDGVTVYDDFAHHPTAIQTTVAGLRTRIGRTNTRILAVLEPRSNTMKLGVMKAQLPASLADADLVFGYGAPSGKDAL
GWNLAEALAPMGDKAQAFNEIDSLVKAVVAAAQPGDQVLVMSNGGFGGVHQKLLDALSARPTEQARGVA
>Mature_469_residues
MHIHILGICGTFMGGLAVLARNAGHTVTGCDAGVYPPMSTQLEAQGIKLIEGWGVEQLDLKPDLFVVGNVVTRGNPLMEE
ILNRGLPYTSGPQWLGEHVLNGKWVLAVAGTHGKTTTTSMLTWLLEDAGMNPGFLIGGVPLNFGVSARLTDSSFFVIEAD
EYDTAFFDKRSKFVHYRPRTAVLNNLEFDHADIFPDLAAIETQFHHLVRTVPGIGRVVSNGREVALDRVLARGCWSEVER
FGVEGGWQALPAEDGVAVDERFAVYHNGERVGVVDWQVQGEHNRMNAIAAIAAARHIGVPPAQAAKSLSTFRNVKRRMEV
RGSVDGVTVYDDFAHHPTAIQTTVAGLRTRIGRTNTRILAVLEPRSNTMKLGVMKAQLPASLADADLVFGYGAPSGKDAL
GWNLAEALAPMGDKAQAFNEIDSLVKAVVAAAQPGDQVLVMSNGGFGGVHQKLLDALSARPTEQARGVA

Specific function: Reutilizes the intact tripeptide L-alanyl-gamma-D- glutamyl-meso-diaminopimelate by linking it to UDP-N-acetylmuramic acid [H]

COG id: COG0773

COG function: function code M; UDP-N-acetylmuramate-alanine ligase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1790680, Length=460, Percent_Identity=59.1304347826087, Blast_Score=546, Evalue=1e-157,
Organism=Escherichia coli, GI1786279, Length=302, Percent_Identity=27.8145695364238, Blast_Score=96, Evalue=6e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005757
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 50424; Mature: 50424

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHIHILGICGTFMGGLAVLARNAGHTVTGCDAGVYPPMSTQLEAQGIKLIEGWGVEQLDL
CEEEEEEHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCEEEEECCCCEEECC
KPDLFVVGNVVTRGNPLMEEILNRGLPYTSGPQWLGEHVLNGKWVLAVAGTHGKTTTTSM
CCCEEEEECEEECCCHHHHHHHHCCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCHHHHH
LTWLLEDAGMNPGFLIGGVPLNFGVSARLTDSSFFVIEADEYDTAFFDKRSKFVHYRPRT
HHHHHHHCCCCCCEEEECCCCCCCCEEEEECCEEEEEECCCCCCHHHHCCCCEEEECCHH
AVLNNLEFDHADIFPDLAAIETQFHHLVRTVPGIGRVVSNGREVALDRVLARGCWSEVER
HHHCCCCCCHHHHCCHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHHHH
FGVEGGWQALPAEDGVAVDERFAVYHNGERVGVVDWQVQGEHNRMNAIAAIAAARHIGVP
HCCCCCCCCCCCCCCCEECCEEEEEECCCEEEEEEEEEECCCCHHHHHHHHHHHHHCCCC
PAQAAKSLSTFRNVKRRMEVRGSVDGVTVYDDFAHHPTAIQTTVAGLRTRIGRTNTRILA
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCCCEEEE
VLEPRSNTMKLGVMKAQLPASLADADLVFGYGAPSGKDALGWNLAEALAPMGDKAQAFNE
EECCCCCCEEEEEEEHHCCCCCCCCCEEEECCCCCCCCCCCCCHHHHHCCCCCHHHHHHH
IDSLVKAVVAAAQPGDQVLVMSNGGFGGVHQKLLDALSARPTEQARGVA
HHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCHHHCCCC
>Mature Secondary Structure
MHIHILGICGTFMGGLAVLARNAGHTVTGCDAGVYPPMSTQLEAQGIKLIEGWGVEQLDL
CEEEEEEHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCEEEEECCCCEEECC
KPDLFVVGNVVTRGNPLMEEILNRGLPYTSGPQWLGEHVLNGKWVLAVAGTHGKTTTTSM
CCCEEEEECEEECCCHHHHHHHHCCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCHHHHH
LTWLLEDAGMNPGFLIGGVPLNFGVSARLTDSSFFVIEADEYDTAFFDKRSKFVHYRPRT
HHHHHHHCCCCCCEEEECCCCCCCCEEEEECCEEEEEECCCCCCHHHHCCCCEEEECCHH
AVLNNLEFDHADIFPDLAAIETQFHHLVRTVPGIGRVVSNGREVALDRVLARGCWSEVER
HHHCCCCCCHHHHCCHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHHHH
FGVEGGWQALPAEDGVAVDERFAVYHNGERVGVVDWQVQGEHNRMNAIAAIAAARHIGVP
HCCCCCCCCCCCCCCCEECCEEEEEECCCEEEEEEEEEECCCCHHHHHHHHHHHHHCCCC
PAQAAKSLSTFRNVKRRMEVRGSVDGVTVYDDFAHHPTAIQTTVAGLRTRIGRTNTRILA
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCCCEEEE
VLEPRSNTMKLGVMKAQLPASLADADLVFGYGAPSGKDALGWNLAEALAPMGDKAQAFNE
EECCCCCCEEEEEEEHHCCCCCCCCCEEEECCCCCCCCCCCCCHHHHHCCCCCHHHHHHH
IDSLVKAVVAAAQPGDQVLVMSNGGFGGVHQKLLDALSARPTEQARGVA
HHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 2843822; 7984428; 8808921 [H]