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The map label for this gene is dctA2 [H]

Identifier: 186477344

GI number: 186477344

Start: 2933230

End: 2934558

Strand: Reverse

Name: dctA2 [H]

Synonym: Bphy_2596

Alternate gene names: 186477344

Gene position: 2934558-2933230 (Counterclockwise)

Preceding gene: 186477346

Following gene: 186477343

Centisome position: 84.35

GC content: 62.15

Gene sequence:

>1329_bases
GTGTTGAAGTTCTTCAATTCGCTGTTTGGCCGGGTGGTGATCGCTCTCGTGGCGGGCATCGTGATCGGCGCGCTGTTTCC
GCATTTCGCACAGTCGCTGCGTCCATTGGGCGACGGCTTCCTCAAGCTGATCAAAATGGTGATCGGTCCGATCGTGTTCT
GCGTCGTCGTCAGCGGCATGGCGCACGCGGGCGATCTGAAGAAAGTAGGGCGCGTCGGCCTGAAGGCCGTCGTGTACTTC
GAGATCATGACGACGATCGCGCTCGTGATCGGCGCGGTGCTCGCGTATGCGACGCGCCCCGGCGTCGGCATGAACATCGA
TCTTCATTCGCTCGATCCCGGGTCGCTCGCGTCATACACCGAGCACGCGAAAAGCCTCAAGGACACAGCGGGCTTCCTGC
TCAAGATCATCCCCGAAACCGCGATCGATGCCTTCGCCAAGGGCGACATCCTGCAGATTCTCGTGTTCTCCGTACTGGTC
GGCTCGGCGCTGTCGCTGCTCGGGCCGCGCGTGCAGCGCGTCAACGGCCTGATCGACGAACTCGCGCAAGTGTTCTTCCG
CGTGATGAGCTTTATCATCAAGCTCGCGCCGCTCGGCGTGCTGGGTGCCATCGCTTTCACGACGGGCACGTATGGCATCG
CGTCGCTCAAGCAGCTCGGCATGCTCGTCGTCGTGTTCTACGCGAGCTGCTTCGTGTTCGTGGTCGTCGTGCTCGGCGTC
GTGATGCGTCTGGCTGGCTTCAGCGTGTTCAAGCTGATTCGCTATTTGCGCGAAGAGCTGTCCATCGTGCTTGGCACCGC
TTCGTCGGACGCCGTGCTGCCGCAAATCATGCGCAAGCTCGAATGGATGGGCGTAAAGGATTCGACGGTCGGTCTCGTGA
TTCCGACAGGCTACTCGTTCAACCTCGACGGCTTTTCGATCTATCTGACGCTCGCCGTCATCTTCATCGCGCAGGCGACG
AACACGCCGCTTTCGACGCACGATCTGATCGTCGTCGTGCTTGTGTCGTTGGTGACGTCGAAGGGCGCGCACGGCATTCC
CGGCTCAGCCATCGTGATTCTCGCCGCGACGCTCTCCGCGATTCCCGCGATTCCCGTCCTCGGCCTCGTGCTGATTCTGC
CCGTCGACTGGTTCGTCGGCATCGCTCGCGCGCTGACCAATCTGATCGGTAACTGCGTTGCAACCGTCGTCGTCGCCGTA
TGGGAGAACGACATCGACAAGGCACGCGCCCGCCGGGTGCTCAATCTCGACAGCGAGTTGCGCTTCGTGCCGACCAGCAC
CGATCTCGACGGCGAGACGGGCCACGCGAACCCGGCGCACGCCGTCTGA

Upstream 100 bases:

>100_bases
ATCGGCTGCCTACTATTCGCCATAACAGCACTGGTCAGACCGGTATGATTGCGGGTCGGATAAGCGTGCTGCTGACCCAA
TTCGTGACCGGGAGAAAGCC

Downstream 100 bases:

>100_bases
TAACGCTTTCCGCCATCTGACCGACCACCAGTGGCAACGCGGCGGACCGCGACGGTCCGCCGCCATTTAACTCAATCGCA
CCTCAATTGACCGAAGACTG

Product: C4-dicarboxylate transporter DctA

Products: orotate [Cytoplasm]; fumarate [Cytoplasm]; malate [Cytoplasm]; Na (I) [Cytoplasm]; succinate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 442; Mature: 442

Protein sequence:

>442_residues
MLKFFNSLFGRVVIALVAGIVIGALFPHFAQSLRPLGDGFLKLIKMVIGPIVFCVVVSGMAHAGDLKKVGRVGLKAVVYF
EIMTTIALVIGAVLAYATRPGVGMNIDLHSLDPGSLASYTEHAKSLKDTAGFLLKIIPETAIDAFAKGDILQILVFSVLV
GSALSLLGPRVQRVNGLIDELAQVFFRVMSFIIKLAPLGVLGAIAFTTGTYGIASLKQLGMLVVVFYASCFVFVVVVLGV
VMRLAGFSVFKLIRYLREELSIVLGTASSDAVLPQIMRKLEWMGVKDSTVGLVIPTGYSFNLDGFSIYLTLAVIFIAQAT
NTPLSTHDLIVVVLVSLVTSKGAHGIPGSAIVILAATLSAIPAIPVLGLVLILPVDWFVGIARALTNLIGNCVATVVVAV
WENDIDKARARRVLNLDSELRFVPTSTDLDGETGHANPAHAV

Sequences:

>Translated_442_residues
MLKFFNSLFGRVVIALVAGIVIGALFPHFAQSLRPLGDGFLKLIKMVIGPIVFCVVVSGMAHAGDLKKVGRVGLKAVVYF
EIMTTIALVIGAVLAYATRPGVGMNIDLHSLDPGSLASYTEHAKSLKDTAGFLLKIIPETAIDAFAKGDILQILVFSVLV
GSALSLLGPRVQRVNGLIDELAQVFFRVMSFIIKLAPLGVLGAIAFTTGTYGIASLKQLGMLVVVFYASCFVFVVVVLGV
VMRLAGFSVFKLIRYLREELSIVLGTASSDAVLPQIMRKLEWMGVKDSTVGLVIPTGYSFNLDGFSIYLTLAVIFIAQAT
NTPLSTHDLIVVVLVSLVTSKGAHGIPGSAIVILAATLSAIPAIPVLGLVLILPVDWFVGIARALTNLIGNCVATVVVAV
WENDIDKARARRVLNLDSELRFVPTSTDLDGETGHANPAHAV
>Mature_442_residues
MLKFFNSLFGRVVIALVAGIVIGALFPHFAQSLRPLGDGFLKLIKMVIGPIVFCVVVSGMAHAGDLKKVGRVGLKAVVYF
EIMTTIALVIGAVLAYATRPGVGMNIDLHSLDPGSLASYTEHAKSLKDTAGFLLKIIPETAIDAFAKGDILQILVFSVLV
GSALSLLGPRVQRVNGLIDELAQVFFRVMSFIIKLAPLGVLGAIAFTTGTYGIASLKQLGMLVVVFYASCFVFVVVVLGV
VMRLAGFSVFKLIRYLREELSIVLGTASSDAVLPQIMRKLEWMGVKDSTVGLVIPTGYSFNLDGFSIYLTLAVIFIAQAT
NTPLSTHDLIVVVLVSLVTSKGAHGIPGSAIVILAATLSAIPAIPVLGLVLILPVDWFVGIARALTNLIGNCVATVVVAV
WENDIDKARARRVLNLDSELRFVPTSTDLDGETGHANPAHAV

Specific function: Responsible for the transport of dicarboxylates such as succinate, fumarate, and malate from the periplasm across the membrane [H]

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family [H]

Homologues:

Organism=Homo sapiens, GI169790839, Length=452, Percent_Identity=26.5486725663717, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI21314632, Length=455, Percent_Identity=26.1538461538462, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI40254478, Length=481, Percent_Identity=25.1559251559252, Blast_Score=106, Evalue=4e-23,
Organism=Homo sapiens, GI4827012, Length=307, Percent_Identity=28.9902280130293, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI66773030, Length=440, Percent_Identity=23.6363636363636, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI5032093, Length=431, Percent_Identity=25.2900232018561, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI223468564, Length=303, Percent_Identity=26.7326732673267, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI223468566, Length=303, Percent_Identity=26.7326732673267, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI194239697, Length=467, Percent_Identity=23.3404710920771, Blast_Score=82, Evalue=9e-16,
Organism=Homo sapiens, GI301601644, Length=224, Percent_Identity=27.6785714285714, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI262359914, Length=386, Percent_Identity=25.9067357512953, Blast_Score=81, Evalue=2e-15,
Organism=Escherichia coli, GI1789947, Length=411, Percent_Identity=50.3649635036496, Blast_Score=421, Evalue=1e-119,
Organism=Escherichia coli, GI1790514, Length=421, Percent_Identity=39.667458432304, Blast_Score=279, Evalue=2e-76,
Organism=Escherichia coli, GI1788024, Length=408, Percent_Identity=22.5490196078431, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1789473, Length=331, Percent_Identity=22.3564954682779, Blast_Score=63, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17537407, Length=426, Percent_Identity=25.1173708920188, Blast_Score=111, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI193206654, Length=408, Percent_Identity=24.7549019607843, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI71996953, Length=428, Percent_Identity=26.1682242990654, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI193206505, Length=462, Percent_Identity=25.7575757575758, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71983106, Length=414, Percent_Identity=24.3961352657005, Blast_Score=96, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI71983099, Length=414, Percent_Identity=24.3961352657005, Blast_Score=96, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17541374, Length=403, Percent_Identity=25.8064516129032, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24583025, Length=427, Percent_Identity=22.9508196721311, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI17137668, Length=427, Percent_Identity=22.9508196721311, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24583023, Length=427, Percent_Identity=22.9508196721311, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI281360481, Length=453, Percent_Identity=25.8278145695364, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI281360483, Length=453, Percent_Identity=25.8278145695364, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI17137666, Length=453, Percent_Identity=25.8278145695364, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991
- InterPro:   IPR018107 [H]

Pfam domain/function: PF00375 SDF [H]

EC number: NA

Molecular weight: Translated: 46936; Mature: 46936

Theoretical pI: Translated: 9.29; Mature: 9.29

Prosite motif: PS00713 NA_DICARBOXYL_SYMP_1 ; PS00714 NA_DICARBOXYL_SYMP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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EEECCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure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EEECCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: orotate [Periplasm]; fumarate [Periplasm]; malate [Periplasm]; Na (I) [Periplasm]; succinate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + orotate [Periplasm] = Na (I) [Cytoplasm] + orotate [Cytoplasm] Na (I) [Periplasm] + fumarate [Periplasm] = Na (I) [Cytoplasm] + fumarate [Cytoplasm] Na (I) [Periplasm] + malate [Periplasm] = Na (I) [Cytoplasm] + malate [Cytoplasm] Na

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]