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The map label for this gene is rlmL [H]

Identifier: 186477285

GI number: 186477285

Start: 2858472

End: 2859749

Strand: Direct

Name: rlmL [H]

Synonym: Bphy_2537

Alternate gene names: 186477285

Gene position: 2858472-2859749 (Clockwise)

Preceding gene: 186477284

Following gene: 186477286

Centisome position: 82.16

GC content: 67.06

Gene sequence:

>1278_bases
ATGTCCTTCGAATTTTTCATCCCCTGCCCGCGCGGCCTCGAAGCCGCGCTCGCCGCCGAACTCGCCGAGATCGCCGCGCG
TCATCTGAAAGGCAAGCCGTTCACCGCCGGCGCGCAAGTGCCAGGCGGCGTGCACTTCAAGGGCGATTGGGCAGCCGGCA
TGGCCGCCAACCTGCATTCGCGGATCGCGAGCCGGGTGCTGTTGAAGATCGCGCATCGTCCGTACCGCAACGAGCAGGAT
ATTTTTGCGCTCGCGCTCGAACAGCGTTGGGAGCAGTGGTTCTCGGCGAACGAGACGCTGCGCGTGGACGTCACCGCGAT
CAAGTCGCCGCTGCGCAGCCTCGAATTTGCGACGCTGCGCGTGAAGGACGCCATCTGCGACCGTCTGCGCGACAAGACGG
GCGCGCGTCCGAGCATCGACACGGGCGCGCCCGATGTGCGCGTGTTCGCGTTCCTCACGGCCACCGACTGCACGCTGTAT
CTCGACACCTCCGGCGAACCGCTGTTCAAGCGCGGCTGGCGTCTGGACAAGGGGGCGGCGCCGCTGCGCGAGAATCTTGC
GGCGGGCATTCTGCGGCTGACGGGCTGGACGCCGGGCACGCCGCTGTACGACCCGATGTGCGGCAGCGGCACTTTCCTCG
CGGAAGCGGCGCAGGTCGCGCTGAGCATCGCACCGGGCGTCGACCGGCGCTTCGCCTTCGAGAAGTTCAAGCAGTACGAC
GTGTCCGCATGGCAGACGCTGAAGGTCGCCGCGCAGGACGCGAAGCGCGCGGCACGCGCGGGCCGCGGCGATCTGCTGAT
CTTCGGCAGCGACATCTCCGACAACATGCTCGACAAGGCGCGCGCGAACTTCGAACGCGCTGGCTTCCCGGCGCTGCCGC
TCAAGCAGATCGACGCACGCAACATGATGCCGCCCGTGAGCGAAGCGGGCATCCTCGTCGCGAACCCGCCGTATGGCGAG
CGGATCGAAGTGCGCGGGCGCGGTCCGCGCGGCGAAATTCGCAACACGGGGCGCGAAGAAGGCGGCTTCCGGCGTGCGGA
AACGGATACACCGGACATGACGGAATTCTTCCAGTCCTTCGGCGATGCGCTCAAGAAGCGCTTTACCGGCTGGCATGCGT
TCGTGCTGACATCGGATCGCAAGCTGCCGGGGCAACTGCGCCTGCGTGAGTCGGCCAAGACGCCGCTGTATAACGGCGCG
CTCGAATGCCGCCTGTTCCGCTTCGATCTGATCGCCGGCAGCGTGCGGCAAAGACCGGCGGCGGATGCCAACGAGTAA

Upstream 100 bases:

>100_bases
GGCGCGGACGCTCCGGCCCGCACATGAATGAACGGCCGCAGGGCACAGCGAGGCGACCGCGTACAATGTCGCCTTAATCA
ACGCCTTTGCGGCAATCTCC

Downstream 100 bases:

>100_bases
GCGGTCTTCTTACCTCCACAAGAAGCGGGCGCCATGCCCGCTTTTTTGTTGCACGTGCACTTTTCGCCCTCGCCAACGCT
ACTGACAGAACGTTGTCAGT

Product: putative RNA methylase

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL [H]

Number of amino acids: Translated: 425; Mature: 424

Protein sequence:

>425_residues
MSFEFFIPCPRGLEAALAAELAEIAARHLKGKPFTAGAQVPGGVHFKGDWAAGMAANLHSRIASRVLLKIAHRPYRNEQD
IFALALEQRWEQWFSANETLRVDVTAIKSPLRSLEFATLRVKDAICDRLRDKTGARPSIDTGAPDVRVFAFLTATDCTLY
LDTSGEPLFKRGWRLDKGAAPLRENLAAGILRLTGWTPGTPLYDPMCGSGTFLAEAAQVALSIAPGVDRRFAFEKFKQYD
VSAWQTLKVAAQDAKRAARAGRGDLLIFGSDISDNMLDKARANFERAGFPALPLKQIDARNMMPPVSEAGILVANPPYGE
RIEVRGRGPRGEIRNTGREEGGFRRAETDTPDMTEFFQSFGDALKKRFTGWHAFVLTSDRKLPGQLRLRESAKTPLYNGA
LECRLFRFDLIAGSVRQRPAADANE

Sequences:

>Translated_425_residues
MSFEFFIPCPRGLEAALAAELAEIAARHLKGKPFTAGAQVPGGVHFKGDWAAGMAANLHSRIASRVLLKIAHRPYRNEQD
IFALALEQRWEQWFSANETLRVDVTAIKSPLRSLEFATLRVKDAICDRLRDKTGARPSIDTGAPDVRVFAFLTATDCTLY
LDTSGEPLFKRGWRLDKGAAPLRENLAAGILRLTGWTPGTPLYDPMCGSGTFLAEAAQVALSIAPGVDRRFAFEKFKQYD
VSAWQTLKVAAQDAKRAARAGRGDLLIFGSDISDNMLDKARANFERAGFPALPLKQIDARNMMPPVSEAGILVANPPYGE
RIEVRGRGPRGEIRNTGREEGGFRRAETDTPDMTEFFQSFGDALKKRFTGWHAFVLTSDRKLPGQLRLRESAKTPLYNGA
LECRLFRFDLIAGSVRQRPAADANE
>Mature_424_residues
SFEFFIPCPRGLEAALAAELAEIAARHLKGKPFTAGAQVPGGVHFKGDWAAGMAANLHSRIASRVLLKIAHRPYRNEQDI
FALALEQRWEQWFSANETLRVDVTAIKSPLRSLEFATLRVKDAICDRLRDKTGARPSIDTGAPDVRVFAFLTATDCTLYL
DTSGEPLFKRGWRLDKGAAPLRENLAAGILRLTGWTPGTPLYDPMCGSGTFLAEAAQVALSIAPGVDRRFAFEKFKQYDV
SAWQTLKVAAQDAKRAARAGRGDLLIFGSDISDNMLDKARANFERAGFPALPLKQIDARNMMPPVSEAGILVANPPYGER
IEVRGRGPRGEIRNTGREEGGFRRAETDTPDMTEFFQSFGDALKKRFTGWHAFVLTSDRKLPGQLRLRESAKTPLYNGAL
ECRLFRFDLIAGSVRQRPAADANE

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA [H]

COG id: COG0116

COG function: function code L; Predicted N6-adenine-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1787181, Length=390, Percent_Identity=31.7948717948718, Blast_Score=202, Evalue=3e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114 [H]

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020 [H]

EC number: =2.1.1.173 [H]

Molecular weight: Translated: 46956; Mature: 46825

Theoretical pI: Translated: 9.86; Mature: 9.86

Prosite motif: PS51165 THUMP ; PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFEFFIPCPRGLEAALAAELAEIAARHLKGKPFTAGAQVPGGVHFKGDWAAGMAANLHS
CCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCHHHHHHHHHH
RIASRVLLKIAHRPYRNEQDIFALALEQRWEQWFSANETLRVDVTAIKSPLRSLEFATLR
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHH
VKDAICDRLRDKTGARPSIDTGAPDVRVFAFLTATDCTLYLDTSGEPLFKRGWRLDKGAA
HHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEECCEEEEEECCCCHHHHCCCCCCCCCC
PLRENLAAGILRLTGWTPGTPLYDPMCGSGTFLAEAAQVALSIAPGVDRRFAFEKFKQYD
HHHHHHHHHHHEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHC
VSAWQTLKVAAQDAKRAARAGRGDLLIFGSDISDNMLDKARANFERAGFPALPLKQIDAR
CHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHCHHHCCCCCCCHHHHCHH
NMMPPVSEAGILVANPPYGERIEVRGRGPRGEIRNTGREEGGFRRAETDTPDMTEFFQSF
CCCCCCCCCCEEEECCCCCCEEEEECCCCCCHHHCCCCCCCCCCCCCCCCCHHHHHHHHH
GDALKKRFTGWHAFVLTSDRKLPGQLRLRESAKTPLYNGALECRLFRFDLIAGSVRQRPA
HHHHHHHHCCEEEEEEECCCCCCCCEEEHHHCCCCCCCCCEEEEEEEEEHHHHHHHCCCC
ADANE
CCCCC
>Mature Secondary Structure 
SFEFFIPCPRGLEAALAAELAEIAARHLKGKPFTAGAQVPGGVHFKGDWAAGMAANLHS
CEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCHHHHHHHHHH
RIASRVLLKIAHRPYRNEQDIFALALEQRWEQWFSANETLRVDVTAIKSPLRSLEFATLR
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHH
VKDAICDRLRDKTGARPSIDTGAPDVRVFAFLTATDCTLYLDTSGEPLFKRGWRLDKGAA
HHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEECCEEEEEECCCCHHHHCCCCCCCCCC
PLRENLAAGILRLTGWTPGTPLYDPMCGSGTFLAEAAQVALSIAPGVDRRFAFEKFKQYD
HHHHHHHHHHHEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHC
VSAWQTLKVAAQDAKRAARAGRGDLLIFGSDISDNMLDKARANFERAGFPALPLKQIDAR
CHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHCHHHCCCCCCCHHHHCHH
NMMPPVSEAGILVANPPYGERIEVRGRGPRGEIRNTGREEGGFRRAETDTPDMTEFFQSF
CCCCCCCCCCEEEECCCCCCEEEEECCCCCCHHHCCCCCCCCCCCCCCCCCHHHHHHHHH
GDALKKRFTGWHAFVLTSDRKLPGQLRLRESAKTPLYNGALECRLFRFDLIAGSVRQRPA
HHHHHHHHCCEEEEEEECCCCCCCCEEEHHHCCCCCCCCCEEEEEEEEEHHHHHHHCCCC
ADANE
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA