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The map label for this gene is ddh [H]

Identifier: 186477178

GI number: 186477178

Start: 2731154

End: 2732161

Strand: Direct

Name: ddh [H]

Synonym: Bphy_2429

Alternate gene names: 186477178

Gene position: 2731154-2732161 (Clockwise)

Preceding gene: 186477177

Following gene: 186477180

Centisome position: 78.5

GC content: 66.17

Gene sequence:

>1008_bases
ATGCGCATGATGCTGTTCAGCAGCCGCCAGTACGACATCGACACCTTCACCGAGGCAAATGCCGCGTTCGGCTACGAGTT
GCACTTTCAGGAGTCGCATCTCGACGCGGACACGGCCGTTCTCGCCGAGGGCTACGATGTCGTGTGTCCGTTCGTCAACG
ACAGCGTCGACGCTGCGACGCTCGAAGCGCTGCACGCAGGCGGCGCGCGGCTCATCGCACTGCGCTCAGCGGGCTTCAAT
CACGTCGATCTCGCGGCGGCGCAGCGGCTCGGCATCGGCGTCGTGCGCGTGCCAGCCTATTCGCCGTACGCGGTCGCCGA
GCATGCGGTCGGACTGATCCTCGCGCTGAACCGCAAGCTCGCGCGCGCTGTCGCGCGCACGCGCGAAGGCGACTTCTCGC
TGAACGGCCTGCTCGGTTTCGATCTGCATGGCAAGACGGTCGGCGTGATCGGCACGGGCATCATCGGCCGCTGCTTTGGG
CGCATCATGGCGGGCTTCGGCATGCAGGTGCTCGCGCACGATCCCGGGCCGCCGGCCACCGAGCTGCTTGCGCTCGGCGG
ACGCTACGTGCCCCTCGATACGCTGCTCGCAGACTCGGATATCGTCAGCCTGCACTGTCCCCTGCTGCCCTCCACGTATC
ACCTGATCGACAGCCACGCACTCGCGAAGATGAAGCGCGGCGCGATGCTGATCAACACGGGGCGCGGCGGGCTCGTCGAA
AGCAATGCGCTGGTCGGCGCGCTCAAGAGCGGGCAGCTGGGACACCTCGGGCTGGATGTGTACGAAGAAGAAGGCGGAAT
TTTCTTCGAGGACCACTCGAATCTGCCGCTGCAGGACGATGTGCTCGCGCGGCTGCTGATGTTCCCCAACGTGATCGTCA
CCGCGCATCAGGCGTTTTTCACGCGCGAGGCGATGACGGAGATAGCACAGACGACGCTTGGCAACGTCGAGGCGTGGCGC
GACGGCACACCGCGCAACGTCGTCGAGGCGCCGCCCGCGAGCGGTTGA

Upstream 100 bases:

>100_bases
GGCGCACGACATGCGTCTCGCGCAGCACGCTTCGTGAGGCGTGCGCCGTGGGCCACGCGTCGCGGACGCCGCACGGGAAG
CTCATCAGGAGAGCCGTGAC

Downstream 100 bases:

>100_bases
AGTTTCGCTGCGGAGCGTGCCTTTTCAAACGCTCATCCTGTCGAGCGCGCCGCGCCGCTCGCATTGGCCGGCGTGGGCCG
CAACGTCGTGCGGGCGTCGT

Product: D-isomer specific 2-hydroxyacid dehydrogenase

Products: pyruvate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MRMMLFSSRQYDIDTFTEANAAFGYELHFQESHLDADTAVLAEGYDVVCPFVNDSVDAATLEALHAGGARLIALRSAGFN
HVDLAAAQRLGIGVVRVPAYSPYAVAEHAVGLILALNRKLARAVARTREGDFSLNGLLGFDLHGKTVGVIGTGIIGRCFG
RIMAGFGMQVLAHDPGPPATELLALGGRYVPLDTLLADSDIVSLHCPLLPSTYHLIDSHALAKMKRGAMLINTGRGGLVE
SNALVGALKSGQLGHLGLDVYEEEGGIFFEDHSNLPLQDDVLARLLMFPNVIVTAHQAFFTREAMTEIAQTTLGNVEAWR
DGTPRNVVEAPPASG

Sequences:

>Translated_335_residues
MRMMLFSSRQYDIDTFTEANAAFGYELHFQESHLDADTAVLAEGYDVVCPFVNDSVDAATLEALHAGGARLIALRSAGFN
HVDLAAAQRLGIGVVRVPAYSPYAVAEHAVGLILALNRKLARAVARTREGDFSLNGLLGFDLHGKTVGVIGTGIIGRCFG
RIMAGFGMQVLAHDPGPPATELLALGGRYVPLDTLLADSDIVSLHCPLLPSTYHLIDSHALAKMKRGAMLINTGRGGLVE
SNALVGALKSGQLGHLGLDVYEEEGGIFFEDHSNLPLQDDVLARLLMFPNVIVTAHQAFFTREAMTEIAQTTLGNVEAWR
DGTPRNVVEAPPASG
>Mature_335_residues
MRMMLFSSRQYDIDTFTEANAAFGYELHFQESHLDADTAVLAEGYDVVCPFVNDSVDAATLEALHAGGARLIALRSAGFN
HVDLAAAQRLGIGVVRVPAYSPYAVAEHAVGLILALNRKLARAVARTREGDFSLNGLLGFDLHGKTVGVIGTGIIGRCFG
RIMAGFGMQVLAHDPGPPATELLALGGRYVPLDTLLADSDIVSLHCPLLPSTYHLIDSHALAKMKRGAMLINTGRGGLVE
SNALVGALKSGQLGHLGLDVYEEEGGIFFEDHSNLPLQDDVLARLLMFPNVIVTAHQAFFTREAMTEIAQTTLGNVEAWR
DGTPRNVVEAPPASG

Specific function: Fermentative Lactate Dehydrogenase. [C]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308577, Length=233, Percent_Identity=33.9055793991416, Blast_Score=138, Evalue=5e-33,
Organism=Homo sapiens, GI61743967, Length=249, Percent_Identity=35.3413654618474, Blast_Score=121, Evalue=8e-28,
Organism=Homo sapiens, GI4557497, Length=249, Percent_Identity=35.3413654618474, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI145580578, Length=249, Percent_Identity=33.3333333333333, Blast_Score=115, Evalue=5e-26,
Organism=Homo sapiens, GI4557499, Length=249, Percent_Identity=33.3333333333333, Blast_Score=115, Evalue=5e-26,
Organism=Homo sapiens, GI145580575, Length=249, Percent_Identity=33.3333333333333, Blast_Score=112, Evalue=4e-25,
Organism=Homo sapiens, GI6912396, Length=323, Percent_Identity=28.4829721362229, Blast_Score=103, Evalue=2e-22,
Organism=Escherichia coli, GI1787645, Length=322, Percent_Identity=51.8633540372671, Blast_Score=338, Evalue=3e-94,
Organism=Escherichia coli, GI87082289, Length=281, Percent_Identity=31.6725978647687, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1789279, Length=260, Percent_Identity=33.0769230769231, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1788660, Length=214, Percent_Identity=32.7102803738318, Blast_Score=75, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17532191, Length=272, Percent_Identity=29.7794117647059, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI25147481, Length=239, Percent_Identity=29.2887029288703, Blast_Score=101, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6322116, Length=287, Percent_Identity=31.7073170731707, Blast_Score=118, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6320925, Length=311, Percent_Identity=30.5466237942122, Blast_Score=117, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6324055, Length=245, Percent_Identity=31.4285714285714, Blast_Score=113, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6324964, Length=212, Percent_Identity=29.7169811320755, Blast_Score=90, Evalue=6e-19,
Organism=Drosophila melanogaster, GI19921140, Length=257, Percent_Identity=29.5719844357977, Blast_Score=124, Evalue=8e-29,
Organism=Drosophila melanogaster, GI24646446, Length=263, Percent_Identity=36.1216730038023, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI24646448, Length=263, Percent_Identity=36.1216730038023, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI24646452, Length=263, Percent_Identity=36.1216730038023, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI24646450, Length=263, Percent_Identity=36.1216730038023, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI62472511, Length=263, Percent_Identity=36.1216730038023, Blast_Score=121, Evalue=8e-28,
Organism=Drosophila melanogaster, GI28574286, Length=267, Percent_Identity=31.8352059925094, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI45552429, Length=262, Percent_Identity=30.9160305343511, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24585514, Length=262, Percent_Identity=30.9160305343511, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI28574282, Length=262, Percent_Identity=30.9160305343511, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI45551003, Length=262, Percent_Identity=30.9160305343511, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI28574284, Length=262, Percent_Identity=30.9160305343511, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI28571528, Length=281, Percent_Identity=31.3167259786477, Blast_Score=98, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24585516, Length=281, Percent_Identity=24.1992882562278, Blast_Score=81, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: 1.1.1.28

Molecular weight: Translated: 35770; Mature: 35770

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: PS00670 D_2_HYDROXYACID_DH_2 ; PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRMMLFSSRQYDIDTFTEANAAFGYELHFQESHLDADTAVLAEGYDVVCPFVNDSVDAAT
CEEEEECCCCCCCCCCCCCCCEEEEEEEEECCCCCCCHHEEECCCEEEEECCCCCCCHHH
LEALHAGGARLIALRSAGFNHVDLAAAQRLGIGVVRVPAYSPYAVAEHAVGLILALNRKL
HHHHHCCCEEEEEEECCCCCHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHH
ARAVARTREGDFSLNGLLGFDLHGKTVGVIGTGIIGRCFGRIMAGFGMQVLAHDPGPPAT
HHHHHHCCCCCEEECCEEEEEECCCEEEEEECCHHHHHHHHHHHCCCCEEEEECCCCCHH
ELLALGGRYVPLDTLLADSDIVSLHCPLLPSTYHLIDSHALAKMKRGAMLINTGRGGLVE
HHHHHCCCCCCHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCEEE
SNALVGALKSGQLGHLGLDVYEEEGGIFFEDHSNLPLQDDVLARLLMFPNVIVTAHQAFF
CCHHEEEECCCCCCEECCEEEECCCCEEEECCCCCCCCHHHHHHHHHCCCCEEEHHHHHH
TREAMTEIAQTTLGNVEAWRDGTPRNVVEAPPASG
HHHHHHHHHHHHCCCCCHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRMMLFSSRQYDIDTFTEANAAFGYELHFQESHLDADTAVLAEGYDVVCPFVNDSVDAAT
CEEEEECCCCCCCCCCCCCCCEEEEEEEEECCCCCCCHHEEECCCEEEEECCCCCCCHHH
LEALHAGGARLIALRSAGFNHVDLAAAQRLGIGVVRVPAYSPYAVAEHAVGLILALNRKL
HHHHHCCCEEEEEEECCCCCHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHH
ARAVARTREGDFSLNGLLGFDLHGKTVGVIGTGIIGRCFGRIMAGFGMQVLAHDPGPPAT
HHHHHHCCCCCEEECCEEEEEECCCEEEEEECCHHHHHHHHHHHCCCCEEEEECCCCCHH
ELLALGGRYVPLDTLLADSDIVSLHCPLLPSTYHLIDSHALAKMKRGAMLINTGRGGLVE
HHHHHCCCCCCHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCEEE
SNALVGALKSGQLGHLGLDVYEEEGGIFFEDHSNLPLQDDVLARLLMFPNVIVTAHQAFF
CCHHEEEECCCCCCEECCEEEECCCCEEEECCCCCCCCHHHHHHHHHCCCCEEEHHHHHH
TREAMTEIAQTTLGNVEAWRDGTPRNVVEAPPASG
HHHHHHHHHHHHCCCCCHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (R)-lactate; NAD+

Specific reaction: (R)-lactate + NAD+ = pyruvate + NADH + H+

General reaction: Redox reaction [C]

Inhibitor: Pyruvate [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]