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The map label for this gene is bcsZ [H]

Identifier: 186477129

GI number: 186477129

Start: 2670365

End: 2671531

Strand: Direct

Name: bcsZ [H]

Synonym: Bphy_2378

Alternate gene names: 186477129

Gene position: 2670365-2671531 (Clockwise)

Preceding gene: 186477125

Following gene: 186477143

Centisome position: 76.75

GC content: 59.47

Gene sequence:

>1167_bases
ATGCGAAGGAAAAGATTACTCACGCAAAGGTTTGCTCGAATCCCAAAATGTGGCGCCGTGAGGCGCCTTGTTGGGCTCGC
GGTGGCTTGCCTGCAGGTCAGCGTTGCCGCACACGCCGATTGTCGCTGGCCCTTATGGGAGCGCTACGCGCAGCAACAGA
TCTCGAAAGACGGACGCATCGTTGAGCCGGGAAAAGGCGATCGCACGACCTCTGAGGCACAGGCCTATGCAATGTTCTTT
GCAGTGGTGGGGGACGATCGGGTGCGATTCGACAGCTTGCTCAACTGGGCGCTCGCTCACACGTGGGCGACGACGGGACA
CCCTCCCGCGTGGCTATGGGCGAAGCGTGCCGACGGCACCTTCGACGTCCTCGACGACAATTCGGCTACGGATGCCGACC
TCTGGATGGCTTATGCAATGCTCGAGGCGGCGAGGCGGTGGTCGGAGCCGCGCTACCGTGCACTTGGTCTGCGGCTGCTC
GATCAGATCACGGCCACCGAGGTCGTGTGGCAGGACGAGACATCAGCCTTTCTCATTCCAGGTCGGCTCGGTTTCCGGTT
GAGTGAACATGCATATTTGGTGAATCCTAGCTACGCGCCGATCCAGCTTCTTCGCCGCTTCATAAAAGAACAGCCGCACG
GACCTTGGGTATCGGTCTTGAACCATCAGGTCGATCTCCTGGTCGCCAGTGCCCCGCAGGGGTTCGTTCCAGATTGGTAT
CGCTTCGACACATTCAAAGGCTACATGCTCGAACCAAGCAAAGGGCCGACCGGTGGCTTTGATGCGGTACGAACGTACAT
GTGGGCGGGCATGCTTGACCCGACGGATCCCGCGCGTGGCGAAGTCATTCCAGCCTTGTCCGGTATGGCGGACCTGGTTG
CACAGACAGGCCGCATGCCGCTTTACGTCAACATCGAATCGGGCGAGACCTTCGATGAAGGTTCAGCCGGGTTTCGGGCT
GCGGTCATTCCATTGTTATTCGTGACCGGGCACCCGAGCGCAGCCAGTCGGCTCGCACAGGAGACGAAAGTCATGCAACA
GGCGCGTGAGTGGGATGGTCTCCATTATTACGATCGCAACCTGCTGTTGTTTGCAGCAGGCTGGCTGGAAGGCAGGTATC
GTTTCGATCACGAGGGCACACTACAACTCAAGCCGTGCAGGCCTTGA

Upstream 100 bases:

>100_bases
AGCGGAGGGAACGAGGAGCGTCTGTAATCTGTTAACGGCACCCTTTCAAGACGCTGAAGTAACCGATAGAAAATCGGTAG
TTCTTGCGCTGACAAGGTGT

Downstream 100 bases:

>100_bases
TCGTCGCAGCCAAGCTACGGGCCGGTGCATGTTCGACAGAACTTTGATATCGCGGCAACCGATCTGCGTCCGCTGTTTGA
TCGCAGAGCCGCCATCGAAC

Product: endo-1,4-D-glucanase

Products: NA

Alternate protein names: Carboxymethylcellulase; CMCase; Cellulase; Endo-1,4-beta-glucanase [H]

Number of amino acids: Translated: 388; Mature: 388

Protein sequence:

>388_residues
MRRKRLLTQRFARIPKCGAVRRLVGLAVACLQVSVAAHADCRWPLWERYAQQQISKDGRIVEPGKGDRTTSEAQAYAMFF
AVVGDDRVRFDSLLNWALAHTWATTGHPPAWLWAKRADGTFDVLDDNSATDADLWMAYAMLEAARRWSEPRYRALGLRLL
DQITATEVVWQDETSAFLIPGRLGFRLSEHAYLVNPSYAPIQLLRRFIKEQPHGPWVSVLNHQVDLLVASAPQGFVPDWY
RFDTFKGYMLEPSKGPTGGFDAVRTYMWAGMLDPTDPARGEVIPALSGMADLVAQTGRMPLYVNIESGETFDEGSAGFRA
AVIPLLFVTGHPSAASRLAQETKVMQQAREWDGLHYYDRNLLLFAAGWLEGRYRFDHEGTLQLKPCRP

Sequences:

>Translated_388_residues
MRRKRLLTQRFARIPKCGAVRRLVGLAVACLQVSVAAHADCRWPLWERYAQQQISKDGRIVEPGKGDRTTSEAQAYAMFF
AVVGDDRVRFDSLLNWALAHTWATTGHPPAWLWAKRADGTFDVLDDNSATDADLWMAYAMLEAARRWSEPRYRALGLRLL
DQITATEVVWQDETSAFLIPGRLGFRLSEHAYLVNPSYAPIQLLRRFIKEQPHGPWVSVLNHQVDLLVASAPQGFVPDWY
RFDTFKGYMLEPSKGPTGGFDAVRTYMWAGMLDPTDPARGEVIPALSGMADLVAQTGRMPLYVNIESGETFDEGSAGFRA
AVIPLLFVTGHPSAASRLAQETKVMQQAREWDGLHYYDRNLLLFAAGWLEGRYRFDHEGTLQLKPCRP
>Mature_388_residues
MRRKRLLTQRFARIPKCGAVRRLVGLAVACLQVSVAAHADCRWPLWERYAQQQISKDGRIVEPGKGDRTTSEAQAYAMFF
AVVGDDRVRFDSLLNWALAHTWATTGHPPAWLWAKRADGTFDVLDDNSATDADLWMAYAMLEAARRWSEPRYRALGLRLL
DQITATEVVWQDETSAFLIPGRLGFRLSEHAYLVNPSYAPIQLLRRFIKEQPHGPWVSVLNHQVDLLVASAPQGFVPDWY
RFDTFKGYMLEPSKGPTGGFDAVRTYMWAGMLDPTDPARGEVIPALSGMADLVAQTGRMPLYVNIESGETFDEGSAGFRA
AVIPLLFVTGHPSAASRLAQETKVMQQAREWDGLHYYDRNLLLFAAGWLEGRYRFDHEGTLQLKPCRP

Specific function: Hydrolyzes carboxymethylcellulose [H]

COG id: COG3405

COG function: function code G; Endoglucanase Y

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 8 (cellulase D) family [H]

Homologues:

Organism=Escherichia coli, GI1789951, Length=362, Percent_Identity=35.9116022099448, Blast_Score=240, Evalue=1e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008928
- InterPro:   IPR012341
- InterPro:   IPR002037
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01270 Glyco_hydro_8 [H]

EC number: =3.2.1.4 [H]

Molecular weight: Translated: 43773; Mature: 43773

Theoretical pI: Translated: 8.00; Mature: 8.00

Prosite motif: PS00812 GLYCOSYL_HYDROL_F8

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRKRLLTQRFARIPKCGAVRRLVGLAVACLQVSVAAHADCRWPLWERYAQQQISKDGRI
CCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCE
VEPGKGDRTTSEAQAYAMFFAVVGDDRVRFDSLLNWALAHTWATTGHPPAWLWAKRADGT
ECCCCCCCCHHHHHHEEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCE
FDVLDDNSATDADLWMAYAMLEAARRWSEPRYRALGLRLLDQITATEVVWQDETSAFLIP
EEEECCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHEEEECCCCEEEEC
GRLGFRLSEHAYLVNPSYAPIQLLRRFIKEQPHGPWVSVLNHQVDLLVASAPQGFVPDWY
CCCCCEECCCEEEECCCCHHHHHHHHHHHHCCCCCHHHHHCCCEEEEEECCCCCCCCCCC
RFDTFKGYMLEPSKGPTGGFDAVRTYMWAGMLDPTDPARGEVIPALSGMADLVAQTGRMP
EECCCCCEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCE
LYVNIESGETFDEGSAGFRAAVIPLLFVTGHPSAASRLAQETKVMQQAREWDGLHYYDRN
EEEEECCCCCCCCCCCCHHHEEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEECCC
LLLFAAGWLEGRYRFDHEGTLQLKPCRP
EEEEEEECCCCCEEECCCCEEEECCCCC
>Mature Secondary Structure
MRRKRLLTQRFARIPKCGAVRRLVGLAVACLQVSVAAHADCRWPLWERYAQQQISKDGRI
CCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCE
VEPGKGDRTTSEAQAYAMFFAVVGDDRVRFDSLLNWALAHTWATTGHPPAWLWAKRADGT
ECCCCCCCCHHHHHHEEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCE
FDVLDDNSATDADLWMAYAMLEAARRWSEPRYRALGLRLLDQITATEVVWQDETSAFLIP
EEEECCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHEEEECCCCEEEEC
GRLGFRLSEHAYLVNPSYAPIQLLRRFIKEQPHGPWVSVLNHQVDLLVASAPQGFVPDWY
CCCCCEECCCEEEECCCCHHHHHHHHHHHHCCCCCHHHHHCCCEEEEEECCCCCCCCCCC
RFDTFKGYMLEPSKGPTGGFDAVRTYMWAGMLDPTDPARGEVIPALSGMADLVAQTGRMP
EECCCCCEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCE
LYVNIESGETFDEGSAGFRAAVIPLLFVTGHPSAASRLAQETKVMQQAREWDGLHYYDRN
EEEEECCCCCCCCCCCCHHHEEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEECCC
LLLFAAGWLEGRYRFDHEGTLQLKPCRP
EEEEEEECCCCCEEECCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]