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The map label for this gene is waaA [H]

Identifier: 186477015

GI number: 186477015

Start: 2536613

End: 2537920

Strand: Reverse

Name: waaA [H]

Synonym: Bphy_2264

Alternate gene names: 186477015

Gene position: 2537920-2536613 (Counterclockwise)

Preceding gene: 186477016

Following gene: 186477001

Centisome position: 72.95

GC content: 67.66

Gene sequence:

>1308_bases
ATGCTAAGGGGCGTCTATAACGCGTTGTGGTGGCTCGTCGCGCCGCTCGCGGTGCTGCGTCTTCTGATCCGTTCGCGCAA
GGAGCGCGGCTATCGCGAACATATCGGCGAGCGCTTCGGTTTCGGGCGCGGCCGCGTGCCCGACGACGATACCCCGCTGA
TCTGGGTGCATGCCGTATCGGTGGGCGAGACGCGCGCGGCGCAGCCGCTGGTCGAAGCGCTGATCAAGGCGCGGCCCGAC
GCGCGCATCCTGCTCACGCACATGACGCCGAGCGGCCGCGCAACGGGCGTGCAGATCTTTGGCGAGCGCGTGTTGCGCTG
CTATCTGCCGTACGACATGCCCCGCGCCGTGCGGCGTTTTCTGAAGGCATGGCGTCCGTCCGTTGGTCTCGTGATGGAAA
CCGAAGTGTGGCCGACGTTGATCGACGAGTGTCGGCGCGCCGACGTGCCGCTCGTGCTGACGAATGCGCGGATGTCGGAG
CGTTCGTACCGGCGCGCGGCGAAGTTCGGTACGGCGACGCGCGGCGTGTTCGGCGGCTTCGCTCGGGTGCTGGCGCAGAG
TCCATCGGATGCAACGCGGCTTTCTTCGCTGGGCGCGCGCAACGTGGCGGTGCTCGGCAATCTCAAGTTCGACATGAATA
CACCGCCTGAACTCGCGGCGCGCGGACATGCGTGGCGCGCGGCGATCGGGCCGCGACCCGTGTGGGTCGCGGCGAGCACG
CGTGAGGGCGAAGAGGAACTGGTACTGCAGGCGTTGGCGGCGCTCGGCATCGACGATGCGCTGCTGGTTCTCGTGCCGCG
TCATCCGCAGCGTTTCAATGAAGTCGCGGCGCTGGTCGAAAAGAAGGGATTGCGCGGCGTGCGGCGTTCAGCCTGGGCGC
CGACGGGCGCGGTCGCGGCGCAAGATAGCGCCGTGCCCGAACTGCCGCGCGACGTGAAGGTGCTGCTCGGCGATTCGATG
GGCGAGCTGGGCGCTTACTATGCTGCGTCGGATGTGGCGTTTATCGGCGGCAGTCTGCTGCCGTTGGGCGGGCAGAATCT
GATCGAGGCATGCGGTGTGGGCGTGCCCGTGCTGATCGGCCCGCATGTGTTCAATTTCACGCAGGCCACGGCCGATGCGG
TTGCTGCGGGCGCGTGCGTGCAGGTGAAAGATCCCGCCGATCTGGCGCGCGTGCTGCGGGAGTTGTTCGAGGACAAGCCT
CGACGCGTTGCAATGGGCGGGGCGGCTTCTGCGTTCGCCGCGCGGCACAGAGGCGCTACCGCGCGAACCGTGGATGTGCT
GACTACGCTGTTGCCGTTGCCAGGCTGA

Upstream 100 bases:

>100_bases
GCGCACTGCCTGTTCCGTCCAATGCATCGCGAGACGCCGCGGCAGCTGCGGCGTCGTCGCGTGTCATCGTGCGCTTCGTG
TCGTCATGCATGGAGGCCCG

Downstream 100 bases:

>100_bases
GCGCTGGCAGTGCTGTTTTTGCCTGCTGCAGGCAAAAAACGACGACCGCGTCGCAGACAAAAAACCTCACCCCACCAGCA
ACCCCTTGCGTTCAATAAAC

Product: 3-deoxy-D-manno-octulosonic-acid transferase

Products: KDO2-lipid IVA; CMP; KDO1-lipid IVA [C]

Alternate protein names: KDO transferase [H]

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MLRGVYNALWWLVAPLAVLRLLIRSRKERGYREHIGERFGFGRGRVPDDDTPLIWVHAVSVGETRAAQPLVEALIKARPD
ARILLTHMTPSGRATGVQIFGERVLRCYLPYDMPRAVRRFLKAWRPSVGLVMETEVWPTLIDECRRADVPLVLTNARMSE
RSYRRAAKFGTATRGVFGGFARVLAQSPSDATRLSSLGARNVAVLGNLKFDMNTPPELAARGHAWRAAIGPRPVWVAAST
REGEEELVLQALAALGIDDALLVLVPRHPQRFNEVAALVEKKGLRGVRRSAWAPTGAVAAQDSAVPELPRDVKVLLGDSM
GELGAYYAASDVAFIGGSLLPLGGQNLIEACGVGVPVLIGPHVFNFTQATADAVAAGACVQVKDPADLARVLRELFEDKP
RRVAMGGAASAFAARHRGATARTVDVLTTLLPLPG

Sequences:

>Translated_435_residues
MLRGVYNALWWLVAPLAVLRLLIRSRKERGYREHIGERFGFGRGRVPDDDTPLIWVHAVSVGETRAAQPLVEALIKARPD
ARILLTHMTPSGRATGVQIFGERVLRCYLPYDMPRAVRRFLKAWRPSVGLVMETEVWPTLIDECRRADVPLVLTNARMSE
RSYRRAAKFGTATRGVFGGFARVLAQSPSDATRLSSLGARNVAVLGNLKFDMNTPPELAARGHAWRAAIGPRPVWVAAST
REGEEELVLQALAALGIDDALLVLVPRHPQRFNEVAALVEKKGLRGVRRSAWAPTGAVAAQDSAVPELPRDVKVLLGDSM
GELGAYYAASDVAFIGGSLLPLGGQNLIEACGVGVPVLIGPHVFNFTQATADAVAAGACVQVKDPADLARVLRELFEDKP
RRVAMGGAASAFAARHRGATARTVDVLTTLLPLPG
>Mature_435_residues
MLRGVYNALWWLVAPLAVLRLLIRSRKERGYREHIGERFGFGRGRVPDDDTPLIWVHAVSVGETRAAQPLVEALIKARPD
ARILLTHMTPSGRATGVQIFGERVLRCYLPYDMPRAVRRFLKAWRPSVGLVMETEVWPTLIDECRRADVPLVLTNARMSE
RSYRRAAKFGTATRGVFGGFARVLAQSPSDATRLSSLGARNVAVLGNLKFDMNTPPELAARGHAWRAAIGPRPVWVAAST
REGEEELVLQALAALGIDDALLVLVPRHPQRFNEVAALVEKKGLRGVRRSAWAPTGAVAAQDSAVPELPRDVKVLLGDSM
GELGAYYAASDVAFIGGSLLPLGGQNLIEACGVGVPVLIGPHVFNFTQATADAVAAGACVQVKDPADLARVLRELFEDKP
RRVAMGGAASAFAARHRGATARTVDVLTTLLPLPG

Specific function: Essential step in lipopolysaccharides biosynthesis. Acts at transfer of 3-deoxy-D-mono octulonic acid (KDO) from CMP-KDO to a tetraacyldisaccharide 1,4'-bisphosphate precursor of lipid A (lipid IVA). Transfers two molecules of KDO to lipid IVA [H]

COG id: COG1519

COG function: function code M; 3-deoxy-D-manno-octulosonic-acid transferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790064, Length=437, Percent_Identity=41.1899313501144, Blast_Score=299, Evalue=3e-82,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296
- InterPro:   IPR007507 [H]

Pfam domain/function: PF00534 Glycos_transf_1; PF04413 Glycos_transf_N [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 47039; Mature: 47039

Theoretical pI: Translated: 10.53; Mature: 10.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRGVYNALWWLVAPLAVLRLLIRSRKERGYREHIGERFGFGRGRVPDDDTPLIWVHAVS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCEEEEEEEE
VGETRAAQPLVEALIKARPDARILLTHMTPSGRATGVQIFGERVLRCYLPYDMPRAVRRF
CCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHH
LKAWRPSVGLVMETEVWPTLIDECRRADVPLVLTNARMSERSYRRAAKFGTATRGVFGGF
HHHHCCCCCEEEECCHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHCCHHHHHHHHH
ARVLAQSPSDATRLSSLGARNVAVLGNLKFDMNTPPELAARGHAWRAAIGPRPVWVAAST
HHHHHCCCCHHHHHHHCCCCCEEEEEEEEECCCCCHHHHHCCCCEEECCCCCCEEEEECC
REGEEELVLQALAALGIDDALLVLVPRHPQRFNEVAALVEKKGLRGVRRSAWAPTGAVAA
CCCHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCC
QDSAVPELPRDVKVLLGDSMGELGAYYAASDVAFIGGSLLPLGGQNLIEACGVGVPVLIG
CCCCCCCCCHHHHHHHCCCHHHHHHHHHHCCHHHHCCCCCCCCCHHHHHHHCCCCCEEEC
PHVFNFTQATADAVAAGACVQVKDPADLARVLRELFEDKPRRVAMGGAASAFAARHRGAT
CCHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHCCCC
ARTVDVLTTLLPLPG
HHHHHHHHHHHCCCC
>Mature Secondary Structure
MLRGVYNALWWLVAPLAVLRLLIRSRKERGYREHIGERFGFGRGRVPDDDTPLIWVHAVS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCEEEEEEEE
VGETRAAQPLVEALIKARPDARILLTHMTPSGRATGVQIFGERVLRCYLPYDMPRAVRRF
CCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHH
LKAWRPSVGLVMETEVWPTLIDECRRADVPLVLTNARMSERSYRRAAKFGTATRGVFGGF
HHHHCCCCCEEEECCHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHCCHHHHHHHHH
ARVLAQSPSDATRLSSLGARNVAVLGNLKFDMNTPPELAARGHAWRAAIGPRPVWVAAST
HHHHHCCCCHHHHHHHCCCCCEEEEEEEEECCCCCHHHHHCCCCEEECCCCCCEEEEECC
REGEEELVLQALAALGIDDALLVLVPRHPQRFNEVAALVEKKGLRGVRRSAWAPTGAVAA
CCCHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCC
QDSAVPELPRDVKVLLGDSMGELGAYYAASDVAFIGGSLLPLGGQNLIEACGVGVPVLIG
CCCCCCCCCHHHHHHHCCCHHHHHHHHHHCCHHHHCCCCCCCCCHHHHHHHCCCCCEEEC
PHVFNFTQATADAVAAGACVQVKDPADLARVLRELFEDKPRRVAMGGAASAFAARHRGAT
CCHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHCCCC
ARTVDVLTTLLPLPG
HHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: KDO1-lipid IVA; CMP-3-deoxy-D-manno-octulosonate; lipid IVA [C]

Specific reaction: KDO1-lipid IVA + CMP-3-deoxy-D-manno-octulosonate = KDO2-lipid IVA + CMP CMP-3-deoxy-D-manno-octulosonate + lipid IVA = KDO1-lipid IVA + CMP [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]