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The map label for this gene is cysB [H]

Identifier: 186476987

GI number: 186476987

Start: 2509137

End: 2510078

Strand: Direct

Name: cysB [H]

Synonym: Bphy_2236

Alternate gene names: 186476987

Gene position: 2509137-2510078 (Clockwise)

Preceding gene: 186476979

Following gene: 186476988

Centisome position: 72.12

GC content: 61.89

Gene sequence:

>942_bases
ATGAACCTGCATCAGTTCCGCTTCGTGCGCGAGGCCGTGCGACAGAATTTCAACCTGACCGAGGCCGCGAAAGCGCTGTA
TACAAGCCAGCCTGGCGTCTCCAAGGCGATCATCGAGCTGGAAGACGAGCTGGGCGTCGAAATCTTCACACGGCACGGCA
AGCGCGTGCGCTCCCTGACGGAGCCGGGGCGGATCATTCTGGCGTCCGTCGAACGGATCCTGCAGGAAGTGGAGAGCCTC
AAGCGGGTCGGTAAAGATTACGCGGCGCAGGACCAGGGCAATCTGGTGATCGCTGCAACCCACACGCAGGCGCGCTATTC
GCTGCCCGCCGCGATCGCCGAGTTCAAGAAGCGCTTTCCCAAGGTGCATCTGTCGATTCTGCAAGGCAGCCCGACCCAGG
TCGCCGAACTGGTGTTGCACGATCAGGCGGACGTGGCGATCGCGACGGAAGCGATCTCGACCTATAAGGAACTGGTGTCG
TTGCCGTGCTTCACCTGGCACCATCTGGCCGTGATGCCCGCCGATCACCCCTTGCTGGAGCGCAAACAGCTGTCGCTCGA
CGATCTGACCCAATACCCGCTGATTACTTACGATAACGCGTTCGCCGGCCGCACCAAGATCAATGACGCGTTCCGCCTGC
GCGGGCTGCATCCCGACATCGTGCTCGAAGCGATTGACGCGGACGTGATCAAGACCTACGTGGAACTGGGGCTGGGCGTC
GGCATCATGGCCGACATCGCGTTCAACGCCGAGCGCGACCGCCATCTGCGCGCGATGCCCGTCGGGCACCTGTTCGGCAG
CAACGTGACACGCGTCGCGCTCAAGCATGGCGCGTATCTGCGCAGCTATGTGTATACGCTCGTCGAACTGCTGTCGCCGA
ACCTGAACCGTCGATTGATCGAGCAGGCGCTCAAGGGCGAGCACGAAACGTATGAGCTTTGA

Upstream 100 bases:

>100_bases
AAAACAGATATCCATATTGGAAAAACTGGACAAATCGTAATAAACTCGCCTTATATTTCAAACGACTAAAAAATTCTTTT
GATATGCGGCGAGGTTATAT

Downstream 100 bases:

>100_bases
GTTTTTTGATTCGCTATAACAACAGCCACCACCGCTGCGTCGGCCGAACTGCTCTAGCCGAACCGCTTTGGCCAAACCGT
TACCGCCAGGAGATCGATCG

Product: transcriptional regulator CysB-like protein

Products: NA

Alternate protein names: Cys regulon transcriptional activator [H]

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MNLHQFRFVREAVRQNFNLTEAAKALYTSQPGVSKAIIELEDELGVEIFTRHGKRVRSLTEPGRIILASVERILQEVESL
KRVGKDYAAQDQGNLVIAATHTQARYSLPAAIAEFKKRFPKVHLSILQGSPTQVAELVLHDQADVAIATEAISTYKELVS
LPCFTWHHLAVMPADHPLLERKQLSLDDLTQYPLITYDNAFAGRTKINDAFRLRGLHPDIVLEAIDADVIKTYVELGLGV
GIMADIAFNAERDRHLRAMPVGHLFGSNVTRVALKHGAYLRSYVYTLVELLSPNLNRRLIEQALKGEHETYEL

Sequences:

>Translated_313_residues
MNLHQFRFVREAVRQNFNLTEAAKALYTSQPGVSKAIIELEDELGVEIFTRHGKRVRSLTEPGRIILASVERILQEVESL
KRVGKDYAAQDQGNLVIAATHTQARYSLPAAIAEFKKRFPKVHLSILQGSPTQVAELVLHDQADVAIATEAISTYKELVS
LPCFTWHHLAVMPADHPLLERKQLSLDDLTQYPLITYDNAFAGRTKINDAFRLRGLHPDIVLEAIDADVIKTYVELGLGV
GIMADIAFNAERDRHLRAMPVGHLFGSNVTRVALKHGAYLRSYVYTLVELLSPNLNRRLIEQALKGEHETYEL
>Mature_313_residues
MNLHQFRFVREAVRQNFNLTEAAKALYTSQPGVSKAIIELEDELGVEIFTRHGKRVRSLTEPGRIILASVERILQEVESL
KRVGKDYAAQDQGNLVIAATHTQARYSLPAAIAEFKKRFPKVHLSILQGSPTQVAELVLHDQADVAIATEAISTYKELVS
LPCFTWHHLAVMPADHPLLERKQLSLDDLTQYPLITYDNAFAGRTKINDAFRLRGLHPDIVLEAIDADVIKTYVELGLGV
GIMADIAFNAERDRHLRAMPVGHLFGSNVTRVALKHGAYLRSYVYTLVELLSPNLNRRLIEQALKGEHETYEL

Specific function: This protein is a positive regulator of gene expression for the cysteine regulon. The inducer for cysB is N-acetylserine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788296, Length=296, Percent_Identity=46.2837837837838, Blast_Score=286, Evalue=1e-78,
Organism=Escherichia coli, GI1787530, Length=304, Percent_Identity=43.75, Blast_Score=282, Evalue=2e-77,
Organism=Escherichia coli, GI157672245, Length=213, Percent_Identity=34.2723004694836, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1788887, Length=245, Percent_Identity=27.7551020408163, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI145693105, Length=245, Percent_Identity=24.8979591836735, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788481, Length=248, Percent_Identity=28.6290322580645, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1790399, Length=254, Percent_Identity=24.0157480314961, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1789204, Length=242, Percent_Identity=26.0330578512397, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI2367136, Length=238, Percent_Identity=23.5294117647059, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1787601, Length=149, Percent_Identity=28.8590604026846, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI87082132, Length=147, Percent_Identity=27.2108843537415, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35180; Mature: 35180

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLHQFRFVREAVRQNFNLTEAAKALYTSQPGVSKAIIELEDELGVEIFTRHGKRVRSLT
CCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCHHHHHHCCHHHHHHC
EPGRIILASVERILQEVESLKRVGKDYAAQDQGNLVIAATHTQARYSLPAAIAEFKKRFP
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCC
KVHLSILQGSPTQVAELVLHDQADVAIATEAISTYKELVSLPCFTWHHLAVMPADHPLLE
CEEEHEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHEEEECCCCCHHH
RKQLSLDDLTQYPLITYDNAFAGRTKINDAFRLRGLHPDIVLEAIDADVIKTYVELGLGV
HHCCCHHHHCCCCEEEECCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCC
GIMADIAFNAERDRHLRAMPVGHLFGSNVTRVALKHGAYLRSYVYTLVELLSPNLNRRLI
HHHHHHHCCCCCCCCEECCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
EQALKGEHETYEL
HHHHCCCCCCCCC
>Mature Secondary Structure
MNLHQFRFVREAVRQNFNLTEAAKALYTSQPGVSKAIIELEDELGVEIFTRHGKRVRSLT
CCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCHHHHHHCCHHHHHHC
EPGRIILASVERILQEVESLKRVGKDYAAQDQGNLVIAATHTQARYSLPAAIAEFKKRFP
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCC
KVHLSILQGSPTQVAELVLHDQADVAIATEAISTYKELVSLPCFTWHHLAVMPADHPLLE
CEEEHEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHEEEECCCCCHHH
RKQLSLDDLTQYPLITYDNAFAGRTKINDAFRLRGLHPDIVLEAIDADVIKTYVELGLGV
HHCCCHHHHCCCCEEEECCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCC
GIMADIAFNAERDRHLRAMPVGHLFGSNVTRVALKHGAYLRSYVYTLVELLSPNLNRRLI
HHHHHHHCCCCCCCCEECCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
EQALKGEHETYEL
HHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]