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The map label for this gene is btuF [H]

Identifier: 186476958

GI number: 186476958

Start: 2481321

End: 2482232

Strand: Direct

Name: btuF [H]

Synonym: Bphy_2207

Alternate gene names: 186476958

Gene position: 2481321-2482232 (Clockwise)

Preceding gene: 186476957

Following gene: 186476965

Centisome position: 71.32

GC content: 66.01

Gene sequence:

>912_bases
ATGCGACTCTGCCGATCACTTCGCGCGCTCGCCACGGCATGCGCCCTCATCATCGCGATGCACGCTCATGCGGCCGTCAC
CGTGGTCGACGACACGGGCGCGCAGGTCATACTCGCGCAGCCCGCGCAACGCGTGATCAGTGTCGCGCCACACGTGACGG
AAATGATCTACGCGGCAGGCGGCGGTTCGAAGCTTGTCGGCGCCGTCACGTACAGCGACTATCCGCCTGAAGCGAAACAG
CTGCCGCGCGTCGGCGACAACAAGGCGCTCGACCTCGAACGCATCGTCGCGTTGAAGCCCGACCTGATCGTCGTGTGGCT
GCATGGCAATGCGCAGCGGCAAACCGACCGCTTGCGCGAACTGCACATTCCCCTCTACTTCAGCGAACCGCATCGCCTCG
ACGACATCGCCGTGTCGCTGACGAAGCTCGGCACGCTGCTCGGCACGACTTCGACGGCCGATGCAGCGGCGCGCAACTTC
CGCCAGCAGATAGCCTGGCTGCGTGAGCGTTACGCGAAGCAACCGGTGGTCAGCGTGTTCTATCAGGTGTGGGACCAGCC
GCTGATGACGCTCAACGGCGAGCACATGATCAGCGATGTGATCGCGCTGTGCGGCGGCCGCAACGTATTCGCGAAGCTCG
AACCGCTGGTGCCGACGGTATCGACGGAAGCCGTGCTGGCTGCGAATCCAGAGGCCATCGTCACGGCCGCGCCCGGCGCC
ACGCAACCGGACCGCCCGTTGCCGTCGCTCGATAAATGGAAGGCGTGGCCCGCGATGACGGCCGTGGCGCGCAACAACCT
GTTCGCGATCGACGGCGACCTGATCAGTCGCCCTGCGCCGCGTATCGCGCAAGGCGCGTCGCAACTGTGCGAAGACCTCG
ATACTGCGCGCGCACGCCGCGCAGGTCGCTAG

Upstream 100 bases:

>100_bases
CCCGTCCCGCGAGCGTGCGGTTCGGCCTGCCCGCAACCGACAGCGAGTGGGACCGCCTCGAAGCCGCGCTGCATCGCACG
ATGACAACGCTCTAGAAACG

Downstream 100 bases:

>100_bases
AACATCACGCGTTCCAGCGCACGATCGACCATTCCTGGCTCGCGTCGTCGCGCCGCAACCAGACGCAACCCGCCATATCG
ATCGACCATTTCAGACAACG

Product: periplasmic binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MRLCRSLRALATACALIIAMHAHAAVTVVDDTGAQVILAQPAQRVISVAPHVTEMIYAAGGGSKLVGAVTYSDYPPEAKQ
LPRVGDNKALDLERIVALKPDLIVVWLHGNAQRQTDRLRELHIPLYFSEPHRLDDIAVSLTKLGTLLGTTSTADAAARNF
RQQIAWLRERYAKQPVVSVFYQVWDQPLMTLNGEHMISDVIALCGGRNVFAKLEPLVPTVSTEAVLAANPEAIVTAAPGA
TQPDRPLPSLDKWKAWPAMTAVARNNLFAIDGDLISRPAPRIAQGASQLCEDLDTARARRAGR

Sequences:

>Translated_303_residues
MRLCRSLRALATACALIIAMHAHAAVTVVDDTGAQVILAQPAQRVISVAPHVTEMIYAAGGGSKLVGAVTYSDYPPEAKQ
LPRVGDNKALDLERIVALKPDLIVVWLHGNAQRQTDRLRELHIPLYFSEPHRLDDIAVSLTKLGTLLGTTSTADAAARNF
RQQIAWLRERYAKQPVVSVFYQVWDQPLMTLNGEHMISDVIALCGGRNVFAKLEPLVPTVSTEAVLAANPEAIVTAAPGA
TQPDRPLPSLDKWKAWPAMTAVARNNLFAIDGDLISRPAPRIAQGASQLCEDLDTARARRAGR
>Mature_303_residues
MRLCRSLRALATACALIIAMHAHAAVTVVDDTGAQVILAQPAQRVISVAPHVTEMIYAAGGGSKLVGAVTYSDYPPEAKQ
LPRVGDNKALDLERIVALKPDLIVVWLHGNAQRQTDRLRELHIPLYFSEPHRLDDIAVSLTKLGTLLGTTSTADAAARNF
RQQIAWLRERYAKQPVVSVFYQVWDQPLMTLNGEHMISDVIALCGGRNVFAKLEPLVPTVSTEAVLAANPEAIVTAAPGA
TQPDRPLPSLDKWKAWPAMTAVARNNLFAIDGDLISRPAPRIAQGASQLCEDLDTARARRAGR

Specific function: Part of the ABC transporter complex BtuCDF involved in vitamin B12 import. Binds vitamin B12 and delivers it to the periplasmic surface of BtuC [H]

COG id: COG0614

COG function: function code P; ABC-type Fe3+-hydroxamate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe/B12 periplasmic-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786353, Length=252, Percent_Identity=36.1111111111111, Blast_Score=145, Evalue=4e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002491 [H]

Pfam domain/function: PF01497 Peripla_BP_2 [H]

EC number: NA

Molecular weight: Translated: 32868; Mature: 32868

Theoretical pI: Translated: 8.62; Mature: 8.62

Prosite motif: PS50983 FE_B12_PBP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLCRSLRALATACALIIAMHAHAAVTVVDDTGAQVILAQPAQRVISVAPHVTEMIYAAG
CHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCEEEEECHHHHHHHHCHHHHHHHHHCC
GGSKLVGAVTYSDYPPEAKQLPRVGDNKALDLERIVALKPDLIVVWLHGNAQRQTDRLRE
CCCEEEEEEEECCCCCCHHHCCCCCCCCCCCHHHHHEECCCEEEEEEECCCHHHHHHHHH
LHIPLYFSEPHRLDDIAVSLTKLGTLLGTTSTADAAARNFRQQIAWLRERYAKQPVVSVF
HCCEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
YQVWDQPLMTLNGEHMISDVIALCGGRNVFAKLEPLVPTVSTEAVLAANPEAIVTAAPGA
HHHHCCHHEEECCHHHHHHHHHHHCCCCHHHECCCCCCCCCCCEEEECCCCEEEEECCCC
TQPDRPLPSLDKWKAWPAMTAVARNNLFAIDGDLISRPAPRIAQGASQLCEDLDTARARR
CCCCCCCCCCHHHHCCHHHHHHHCCCEEEECCHHHCCCCCHHHHHHHHHHHHHHHHHHHH
AGR
CCC
>Mature Secondary Structure
MRLCRSLRALATACALIIAMHAHAAVTVVDDTGAQVILAQPAQRVISVAPHVTEMIYAAG
CHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCEEEEECHHHHHHHHCHHHHHHHHHCC
GGSKLVGAVTYSDYPPEAKQLPRVGDNKALDLERIVALKPDLIVVWLHGNAQRQTDRLRE
CCCEEEEEEEECCCCCCHHHCCCCCCCCCCCHHHHHEECCCEEEEEEECCCHHHHHHHHH
LHIPLYFSEPHRLDDIAVSLTKLGTLLGTTSTADAAARNFRQQIAWLRERYAKQPVVSVF
HCCEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
YQVWDQPLMTLNGEHMISDVIALCGGRNVFAKLEPLVPTVSTEAVLAANPEAIVTAAPGA
HHHHCCHHEEECCHHHHHHHHHHHCCCCHHHECCCCCCCCCCCEEEECCCCEEEEECCCC
TQPDRPLPSLDKWKAWPAMTAVARNNLFAIDGDLISRPAPRIAQGASQLCEDLDTARARR
CCCCCCCCCCHHHHCCHHHHHHHCCCEEEECCHHHCCCCCHHHHHHHHHHHHHHHHHHHH
AGR
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA