| Definition | Burkholderia phymatum STM815 chromosome chromosome 1, complete sequence. |
|---|---|
| Accession | NC_010622 |
| Length | 3,479,187 |
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The map label for this gene is nadE [H]
Identifier: 186476920
GI number: 186476920
Start: 2430833
End: 2432890
Strand: Reverse
Name: nadE [H]
Synonym: Bphy_2169
Alternate gene names: 186476920
Gene position: 2432890-2430833 (Counterclockwise)
Preceding gene: 186476924
Following gene: 186476919
Centisome position: 69.93
GC content: 64.48
Gene sequence:
>2058_bases ATGAAAGGCAATTTCTTCAACCTGTATTCGCATCGTTTCGCGCGCGTCGCGGTGGGCGTGCCGCGCTGCCGTGTCGCGGA CCCGGCCTTCAACGCCGACGAAACGCTCAGGCTCGCACACGAGGCGGCCGCGCAAGGCGCGGCGCTCGTCGCGTTTCCCG AACTCGGCTTGCCCGCCTACACATGCGACGACCTCTTTCATCAGCGCGCGCTGCTCGATGCATGCAAGACAGCGCTGGCA AAGATCGTCGACGCATCGAAGAAGCTGCCCGTCGCACTGATCGTCGGCATGCCGCTGCAGGTGGATCACAGCCTGTACAA CTGCGCTATCGTCGTCGCGCGCGGCGCCGTGCTGGGCGCCGTGCCGAAGACCTATCTGCCGAACTACGGCGAGTTCTACG AAGCGCGTCAGTACGCGCCTGCCGATTGCGCCGTCGCGCGCGACATCGATCTGCTCGGCCAGCGCGTGCCGTTCGGTGCG TCGCTGCTGTTTGAACTGACCGACGTGCCCGATTTCCGCTTTCACGTCGAGATCTGCGAGGACGTGTGGGTGCCCATTCC GCCGTCGTCGTTCGCGTCGCTCGCGGGCGCAACGGTGCTCGTGAATCTGTCGGCGTCGAACGTGGTGGTCGGCAAGTCGG GCTACCGGCATCAGCTGGTGGGGCAGCAGTCGGCGCGCTGCCTGGCGGCGTATCTGTACACGTCGGCGGGACGCGGCGAG TCGTCGACGGACATGGCGTGGGACGGCCAGGCGTTGATCTATGAAAACGGCGAGATGCTCGCCGAGTCGGAGCGTTTTCT CGACGACTCGCACATGATCTTCGCTGACATCGATCTCGAACGGCTGTCGCACGAACGCATGCGCCAGACCACGTTCGGCA TGTCGGTGCAGCGTCACAAGGACGAGGCAGCGAAGTTCCAGGTCGTGCGCGTCGCGATTGGCCTCGACGCTTCGCAGGCG CTGCCGCTCGCGCGCAAGGTCGAGCGCTTCCCATACGTGCCCGCCGACTCGCGCCGCCGCGACGAGCGCTGCAACGAGGT CTACAACATCCAGGTGCAGGCGCTCGTGCAACGGCTGTCGGCGTCGAATATCCAGAAGGTCGTGATCGGCGTGTCGGGCG GGCTGGATTCGACGCATGCGCTGCTCGTTTGTGCGAAGGCGATGGACCGGCTCGGGCTGCCGCGCACCCACATCGTCGGC GTGACGATGCCGGGTTTCGCGACCAGCGACCGCACGCTCAAGCAGGCGCGCGAACTGATGGACGTGGTCGGCTGCACGCC GATGGAAATCGATATCCGGCCAAGCTGCGAACAGATGCTGAAAGATCTCGGCCATCCCTATTCGGCGGGCGAGGCGAAGT ACGACATTACGTTCGAAAACGTGCAGGCGGGCGAGCGCACGAACCATCTGTTCCGTCTCGCCAACTTCCATAACGGCATC GTGATCGGCACGGGCGATCTGAGCGAACTCGCACTGGGCTGGTGCACGTACGGTGTCGGCGATCACATGTCGCACTACAG CGTGAACGCAAGCGTGCCGAAGACGCTGATCACACATCTGGTGCGCTGGGTCGCGGAGACGGGTGCCGTCGGCAGGAGCG GCTCGGATACGCTGCTTAACGTCCTCGCGACGGAGATCAGCCCCGAACTGGTGCCGGGCAAATCGGCGGGCGCGATCGAG CAGAAGACGGAGTCGTTCATCGGCCCGTATGAACTGCAGGACTTCAATCTGTTCTACATCCTGCGCTACGGCTTTGCGCC GTCCAAGGTCGCATTCCTCGCGCTGCATGCATGGCGCGACAAGGAGCAGGGCGGCTGGCCGGAAGGCGCACACGTCACGC GCAACGAATACGATCTCGCCGCGATCAAGCGCAATCTCGGCATCTTTTTGTGGCGCTTTTTCAAGACTAGCCAGTTCAAG CGCACCTGCGTGCCGAATGCGCCGAAGGTCGGCTCGGGCGGCTCGCTGTCGCCGCGCGGCGACTGGCGCGCACCGAGCGA TTCGGAAGCGGCCGTGTGGACCGATGATCTTGCAACGGTTCCGGACAAAGCCGCCTGA
Upstream 100 bases:
>100_bases GAGACCTTGAATGCGCTTACGGCTTATCATTTACGCTGTTCCACAGCCGCCGTTTCGCGCGCGGCCGTCTGCAGGCGTGC GCGCATGAGGAATCTCTGAC
Downstream 100 bases:
>100_bases AACCAGTCGTTATCTGCCGTTGCGCACGTGTGCGCACGCATTGTCATGCCCGAGGCTGGCAACGTGTGCGGTGCGGCGTA GAATCAACGCATCTGTACCT
Product: NAD synthetase
Products: NA
Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]
Number of amino acids: Translated: 685; Mature: 685
Protein sequence:
>685_residues MKGNFFNLYSHRFARVAVGVPRCRVADPAFNADETLRLAHEAAAQGAALVAFPELGLPAYTCDDLFHQRALLDACKTALA KIVDASKKLPVALIVGMPLQVDHSLYNCAIVVARGAVLGAVPKTYLPNYGEFYEARQYAPADCAVARDIDLLGQRVPFGA SLLFELTDVPDFRFHVEICEDVWVPIPPSSFASLAGATVLVNLSASNVVVGKSGYRHQLVGQQSARCLAAYLYTSAGRGE SSTDMAWDGQALIYENGEMLAESERFLDDSHMIFADIDLERLSHERMRQTTFGMSVQRHKDEAAKFQVVRVAIGLDASQA LPLARKVERFPYVPADSRRRDERCNEVYNIQVQALVQRLSASNIQKVVIGVSGGLDSTHALLVCAKAMDRLGLPRTHIVG VTMPGFATSDRTLKQARELMDVVGCTPMEIDIRPSCEQMLKDLGHPYSAGEAKYDITFENVQAGERTNHLFRLANFHNGI VIGTGDLSELALGWCTYGVGDHMSHYSVNASVPKTLITHLVRWVAETGAVGRSGSDTLLNVLATEISPELVPGKSAGAIE QKTESFIGPYELQDFNLFYILRYGFAPSKVAFLALHAWRDKEQGGWPEGAHVTRNEYDLAAIKRNLGIFLWRFFKTSQFK RTCVPNAPKVGSGGSLSPRGDWRAPSDSEAAVWTDDLATVPDKAA
Sequences:
>Translated_685_residues MKGNFFNLYSHRFARVAVGVPRCRVADPAFNADETLRLAHEAAAQGAALVAFPELGLPAYTCDDLFHQRALLDACKTALA KIVDASKKLPVALIVGMPLQVDHSLYNCAIVVARGAVLGAVPKTYLPNYGEFYEARQYAPADCAVARDIDLLGQRVPFGA SLLFELTDVPDFRFHVEICEDVWVPIPPSSFASLAGATVLVNLSASNVVVGKSGYRHQLVGQQSARCLAAYLYTSAGRGE SSTDMAWDGQALIYENGEMLAESERFLDDSHMIFADIDLERLSHERMRQTTFGMSVQRHKDEAAKFQVVRVAIGLDASQA LPLARKVERFPYVPADSRRRDERCNEVYNIQVQALVQRLSASNIQKVVIGVSGGLDSTHALLVCAKAMDRLGLPRTHIVG VTMPGFATSDRTLKQARELMDVVGCTPMEIDIRPSCEQMLKDLGHPYSAGEAKYDITFENVQAGERTNHLFRLANFHNGI VIGTGDLSELALGWCTYGVGDHMSHYSVNASVPKTLITHLVRWVAETGAVGRSGSDTLLNVLATEISPELVPGKSAGAIE QKTESFIGPYELQDFNLFYILRYGFAPSKVAFLALHAWRDKEQGGWPEGAHVTRNEYDLAAIKRNLGIFLWRFFKTSQFK RTCVPNAPKVGSGGSLSPRGDWRAPSDSEAAVWTDDLATVPDKAA >Mature_685_residues MKGNFFNLYSHRFARVAVGVPRCRVADPAFNADETLRLAHEAAAQGAALVAFPELGLPAYTCDDLFHQRALLDACKTALA KIVDASKKLPVALIVGMPLQVDHSLYNCAIVVARGAVLGAVPKTYLPNYGEFYEARQYAPADCAVARDIDLLGQRVPFGA SLLFELTDVPDFRFHVEICEDVWVPIPPSSFASLAGATVLVNLSASNVVVGKSGYRHQLVGQQSARCLAAYLYTSAGRGE SSTDMAWDGQALIYENGEMLAESERFLDDSHMIFADIDLERLSHERMRQTTFGMSVQRHKDEAAKFQVVRVAIGLDASQA LPLARKVERFPYVPADSRRRDERCNEVYNIQVQALVQRLSASNIQKVVIGVSGGLDSTHALLVCAKAMDRLGLPRTHIVG VTMPGFATSDRTLKQARELMDVVGCTPMEIDIRPSCEQMLKDLGHPYSAGEAKYDITFENVQAGERTNHLFRLANFHNGI VIGTGDLSELALGWCTYGVGDHMSHYSVNASVPKTLITHLVRWVAETGAVGRSGSDTLLNVLATEISPELVPGKSAGAIE QKTESFIGPYELQDFNLFYILRYGFAPSKVAFLALHAWRDKEQGGWPEGAHVTRNEYDLAAIKRNLGIFLWRFFKTSQFK RTCVPNAPKVGSGGSLSPRGDWRAPSDSEAAVWTDDLATVPDKAA
Specific function: Can use both glutamine or ammonia as a nitrogen source [H]
COG id: COG0171
COG function: function code H; NAD synthase
Gene ontology:
Cell location: Cytoplasmic
Metaboloic importance: NA
Operon status: Not Known
Operon components: None
Similarity: Contains 1 CN hydrolase domain [H]
Homologues:
Organism=Homo sapiens, GI41393551, Length=524, Percent_Identity=24.4274809160305, Blast_Score=108, Evalue=2e-23, Organism=Caenorhabditis elegans, GI17538756, Length=277, Percent_Identity=31.4079422382672, Blast_Score=114, Evalue=1e-25, Organism=Saccharomyces cerevisiae, GI6321865, Length=274, Percent_Identity=30.6569343065693, Blast_Score=109, Evalue=1e-24, Organism=Drosophila melanogaster, GI24641841, Length=286, Percent_Identity=27.2727272727273, Blast_Score=85, Evalue=2e-16, Organism=Drosophila melanogaster, GI24641843, Length=286, Percent_Identity=27.2727272727273, Blast_Score=85, Evalue=2e-16,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR014445 - InterPro: IPR022310 - InterPro: IPR003694 - InterPro: IPR003010 - InterPro: IPR014729 [H]
Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]
EC number: =6.3.5.1 [H]
Molecular weight: Translated: 75056; Mature: 75056
Theoretical pI: Translated: 6.64; Mature: 6.64
Prosite motif: PS50263 CN_HYDROLASE
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.9 %Cys (Translated Protein) 1.9 %Met (Translated Protein) 3.8 %Cys+Met (Translated Protein) 1.9 %Cys (Mature Protein) 1.9 %Met (Mature Protein) 3.8 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MKGNFFNLYSHRFARVAVGVPRCRVADPAFNADETLRLAHEAAAQGAALVAFPELGLPAY CCCCEEEHHHHHHHHHHCCCCCCEECCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCC TCDDLFHQRALLDACKTALAKIVDASKKLPVALIVGMPLQVDHSLYNCAIVVARGAVLGA HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEECCHHHHEEEEEECCHHHHC VPKTYLPNYGEFYEARQYAPADCAVARDIDLLGQRVPFGASLLFELTDVPDFRFHVEICE CCHHHCCCCHHHHHHHCCCCCCHHHHHCHHHHCCCCCCCHHHHEEECCCCCCEEEEEEHH DVWVPIPPSSFASLAGATVLVNLSASNVVVGKSGYRHQLVGQQSARCLAAYLYTSAGRGE CCCCCCCCHHHHHHCCCEEEEEECCCEEEECCCCCCCHHCCCHHHHHHHHHHHHCCCCCC SSTDMAWDGQALIYENGEMLAESERFLDDSHMIFADIDLERLSHERMRQTTFGMSVQRHK CCCCCEECCCEEEEECCCHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHCCHHHHHH DEAAKFQVVRVAIGLDASQALPLARKVERFPYVPADSRRRDERCNEVYNIQVQALVQRLS HHHHHEEEEEEEECCCHHHCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH ASNIQKVVIGVSGGLDSTHALLVCAKAMDRLGLPRTHIVGVTMPGFATSDRTLKQARELM HCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHH DVVGCTPMEIDIRPSCEQMLKDLGHPYSAGEAKYDITFENVQAGERTNHLFRLANFHNGI HHHCCCCEEEECCCCHHHHHHHCCCCCCCCCEEEEEEECCCCCCCHHHHHEEEECCCCCE VIGTGDLSELALGWCTYGVGDHMSHYSVNASVPKTLITHLVRWVAETGAVGRSGSDTLLN EEECCCHHHHHHHHHHCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHH VLATEISPELVPGKSAGAIEQKTESFIGPYELQDFNLFYILRYGFAPSKVAFLALHAWRD HHHHHCCCCCCCCCCCCCHHHHHHHCCCCEEECCCCEEEEEEECCCCHHHHHHHHHHHCC KEQGGWPEGAHVTRNEYDLAAIKRNLGIFLWRFFKTSQFKRTCVPNAPKVGSGGSLSPRG CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCCCCCCC DWRAPSDSEAAVWTDDLATVPDKAA CCCCCCCCCCEEEECCHHCCCCCCC >Mature Secondary Structure MKGNFFNLYSHRFARVAVGVPRCRVADPAFNADETLRLAHEAAAQGAALVAFPELGLPAY CCCCEEEHHHHHHHHHHCCCCCCEECCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCC TCDDLFHQRALLDACKTALAKIVDASKKLPVALIVGMPLQVDHSLYNCAIVVARGAVLGA HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEECCHHHHEEEEEECCHHHHC VPKTYLPNYGEFYEARQYAPADCAVARDIDLLGQRVPFGASLLFELTDVPDFRFHVEICE CCHHHCCCCHHHHHHHCCCCCCHHHHHCHHHHCCCCCCCHHHHEEECCCCCCEEEEEEHH DVWVPIPPSSFASLAGATVLVNLSASNVVVGKSGYRHQLVGQQSARCLAAYLYTSAGRGE CCCCCCCCHHHHHHCCCEEEEEECCCEEEECCCCCCCHHCCCHHHHHHHHHHHHCCCCCC SSTDMAWDGQALIYENGEMLAESERFLDDSHMIFADIDLERLSHERMRQTTFGMSVQRHK CCCCCEECCCEEEEECCCHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHCCHHHHHH DEAAKFQVVRVAIGLDASQALPLARKVERFPYVPADSRRRDERCNEVYNIQVQALVQRLS HHHHHEEEEEEEECCCHHHCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH ASNIQKVVIGVSGGLDSTHALLVCAKAMDRLGLPRTHIVGVTMPGFATSDRTLKQARELM HCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHH DVVGCTPMEIDIRPSCEQMLKDLGHPYSAGEAKYDITFENVQAGERTNHLFRLANFHNGI HHHCCCCEEEECCCCHHHHHHHCCCCCCCCCEEEEEEECCCCCCCHHHHHEEEECCCCCE VIGTGDLSELALGWCTYGVGDHMSHYSVNASVPKTLITHLVRWVAETGAVGRSGSDTLLN EEECCCHHHHHHHHHHCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHH VLATEISPELVPGKSAGAIEQKTESFIGPYELQDFNLFYILRYGFAPSKVAFLALHAWRD HHHHHCCCCCCCCCCCCCHHHHHHHCCCCEEECCCCEEEEEEECCCCHHHHHHHHHHHCC KEQGGWPEGAHVTRNEYDLAAIKRNLGIFLWRFFKTSQFKRTCVPNAPKVGSGGSLSPRG CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCCCCCCC DWRAPSDSEAAVWTDDLATVPDKAA CCCCCCCCCCEEEECCHHCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 12788972 [H]